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28 条记录 (耗时 0.04 秒)

    Production of Oligosaccharides By Microorganisms
    5.
    发明申请
    Production of Oligosaccharides By Microorganisms 审中-公开
    微生物生产寡糖

    公开(公告)号：US20080145899A1

    公开(公告)日：2008-06-19

    申请号：US11661621

    申请日：2005-09-19

    申请人： Karl Johnson ,  Noel J. Byrne ,  Shawn DeFrees

    发明人： Karl Johnson ,  Noel J. Byrne ,  Shawn DeFrees

    IPC分类号： C12P19/18 ,  C12P19/12

    CPC分类号： C12P19/02 ,  C12P13/02 ,  C12P19/04

    摘要： The present invention relates to the enzymatic synthesis of oligosaccharides, including sialylated product saccharides. In particular, it relates to the use of recombinant cells to take up low cost precursors such as glucose, pyruvate and N-actyl-glucosamine, and to synthesize activated sugar moieties that are used in oligosaccharide synthesis. The methods make possible the synthesis of many oligosaccharides using microorganisms and readily available, relatively inexpensive starting materials.

    摘要翻译： 本发明涉及寡糖的酶促合成，包括唾液酸化产物糖。 特别地，本发明涉及使用重组细胞来摄取低成本前体如葡萄糖，丙酮酸和N-酰胺基葡糖胺，以及合成用于寡糖合成的活性糖部分。 该方法使得可以使用微生物和易于获得的相对便宜的起始材料合成许多寡糖。

    Methods of Refolding Mammalian Glycosyltranferases
    7.
    发明申请
    Methods of Refolding Mammalian Glycosyltranferases 有权
    复制哺乳动物糖基转移酶的方法

    公开(公告)号：US20070269879A1

    公开(公告)日：2007-11-22

    申请号：US10587769

    申请日：2005-02-04

    申请人： Sami Saribas ,  David Hakes ,  Scott Willett ,  Karl Johnson ,  Daniel Bezila ,  Shawn Defrees

    发明人： Sami Saribas ,  David Hakes ,  Scott Willett ,  Karl Johnson ,  Daniel Bezila ,  Shawn Defrees

    IPC分类号： C12P21/06 ,  C12N9/10

    CPC分类号： C12N9/1048

    摘要： The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases.

    摘要翻译： 本发明提供了在细菌细胞中产生的哺乳动物糖基转移酶重折叠的方法，以及使用这种重折叠的糖基转移酶（包括具有增强的重折叠能力的糖基转移酶突变体）的方法。 本发明还提供了在单个容器中重折叠多于一种糖基转移酶的方法，使用这种重折叠的糖基转移酶的方法和包含重折叠的糖基转移酶的反应混合物。

    BATTERY PACK WITH OPTIMIZED MECHANICAL, ELECTRICAL, AND THERMAL MANAGEMENT
    9.
    发明申请
    BATTERY PACK WITH OPTIMIZED MECHANICAL, ELECTRICAL, AND THERMAL MANAGEMENT 审中-公开
    具有优化机械，电气和热管理的电池组

    公开(公告)号：US20100136405A1

    公开(公告)日：2010-06-03

    申请号：US12623346

    申请日：2009-11-20

    申请人： Karl Johnson ,  Paul Durkee

    发明人： Karl Johnson ,  Paul Durkee

    IPC分类号： H01M10/50 ,  H01M6/00

    CPC分类号： H01M2/105 ,  H01M10/0525 ,  H01M10/4207 ,  H01M10/613 ,  H01M10/6557 ,  H01M10/656 ,  H01M10/6567 ,  Y10T29/49108

    摘要： Disclosed is a multi-cell battery pack system with integrated thermal and electrical management that includes a plurality of cells, a plurality of cradles that each define a channel that extends through the length of the cradle and having an external surface that mechanically positions each of the cells radially around and parallel to the channel, that exchanges heat with the cells and that electrically couples with each of the cells, and an endplate that, when the cradle is mechanically coupled to the endplate, the channel of the cradle is connected to a fluid circuit that routes thermally conductive fluid through the channel to exchange heat with the channel and the cells are connected to an electrical circuit.

    摘要翻译： 公开了一种具有集成的热和电管理的多单元电池组系统，其包括多个单元，多个支架各自限定延伸穿过支架的长度的通道，并具有外表面， 电池在通道周围并且平行于通道，与电池交换热量并与每个电池电耦合;以及端板，当支架机械地联接到端板时，支架的通道连接到流体 将导热流体通过通道传导以与通道交换热量的电路和电池连接到电路。

    Cyclodextrin affinity purification
    10.
    发明申请
    Cyclodextrin affinity purification 审中-公开
    环糊精亲和纯化

    公开(公告)号：US20070105192A1

    公开(公告)日：2007-05-10

    申请号：US10555123

    申请日：2004-05-05

    申请人： Joseph Villafranca ,  David Hakes ,  Karl Johnson ,  Walter Willett ,  Chester Meyers

    发明人： Joseph Villafranca ,  David Hakes ,  Karl Johnson ,  Walter Willett ,  Chester Meyers

    IPC分类号： C12P21/06 ,  C12P19/62 ,  C12P19/28 ,  C12P19/04 ,  C12N9/10

    CPC分类号： C07H21/04 ,  C12N11/10 ,  C12P19/04 ,  C12P21/005

    摘要： A method of immobilizing a molecular species that include a starch-binding domain is provided. There also is provided a material upon which the molecular species is immobilized, and a material that is capable of immobilizing the species The method includes binding the species to a solid support, e.g., membranes, chromatographic supports and the like. The immobilized species is optionally purified by the method of the invention. Alternatively, the immobilized species is use in another method, such as in a synthesis as a synthetic reagent, or to purify another species that has an affinity for the immobilized species. Exemplary immobilized molecular species include bioactive agents, and biomolecules.

    摘要翻译： 提供了固定包含淀粉结合结构域的分子种的方法。 还提供了固定有分子种类的材料和能够固定物种的材料。该方法包括将物质结合到固体支持物，例如膜，色谱载体等。 任选地通过本发明的方法纯化固定化的物质。 或者，固定化物质用于另一种方法，例如在作为合成试剂的合成中，或用于纯化对固定化物质具有亲和性的另一物质。 示例性的固定化分子种类包括生物活性剂和生物分子。