公开(公告)号：US07901558B2

公开(公告)日：2011-03-08

申请号：US11278975

申请日：2006-04-07

申请人： Mario Curcio ,  Martin Kopp

发明人： Mario Curcio ,  Martin Kopp

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44773 ,  G01N27/44795

摘要： Described is a process for the separation of a sample mixture for analytical reason based on two-dimensional gel electrophoresis. The method is involving a first separation in a first gel strip on the basis of isoelectric points and a second separation in a second gel on the basis of molecular size. When starting the separation in the second dimension the buffer solution for transferring the compounds separated in the first dimension into the second dimension gel is containing sodium dodecyl-sulfate (SDS) and by applying an electric field the SDS migrates electrokinetically into the first gel strip, and the compounds are being complexed simultaneously with SDS.

摘要翻译： 描述了基于二维凝胶电泳的用于分析原因的样品混合物的分离方法。 该方法涉及基于等电点在第一凝胶条中的第一分离，并且基于分子大小在第二凝胶中进行第二次分离。 当在第二维开始分离时，用于将在第一维中分离的化合物转移到第二维凝胶中的缓冲溶液含有十二烷基硫酸钠（SDS），并且通过施加电场，SDS电动迁移到第一凝胶条中， 并且化合物与SDS同时复合。

公开(公告)号：US20060226010A1

公开(公告)日：2006-10-12

申请号：US11278975

申请日：2006-04-07

申请人： Mario Curcio ,  Martin Kopp

发明人： Mario Curcio ,  Martin Kopp

IPC分类号： B01D61/42 ,  B01D57/02 ,  G01N27/00

CPC分类号： G01N27/44773 ,  G01N27/44795

摘要： Described is a process for the separation of a sample mixture for analytical reason based on two-dimensional gel electrophoresis. The method is involving a first separation in a first gel strip on the basis of isoelectric points and a second separation in a second gel on the basis of molecular size. When starting the separation in the second dimension the buffer solution for transferring the compounds separated in the first dimension into the second dimension gel is containing sodium dodecyl-sulfate (SDS) and by applying an electric field the SDS migrates electrokinetically into the first gel strip, and the compounds are being complexed simultaneously with SDS.

摘要翻译： 描述了基于二维凝胶电泳的用于分析原因的样品混合物的分离方法。 该方法涉及基于等电点在第一凝胶条中的第一分离，并且基于分子大小在第二凝胶中进行第二次分离。 当在第二维开始分离时，用于将在第一维中分离的化合物转移到第二维凝胶中的缓冲溶液含有十二烷基硫酸钠（SDS），并且通过施加电场，SDS电动迁移到第一凝胶条中， 并且化合物与SDS同时复合。

公开(公告)号：US20070144907A1

公开(公告)日：2007-06-28

申请号：US11643729

申请日：2006-12-21

申请人： Martin Kopp ,  Roger Sandoz ,  Mario Curcio ,  Michael Glauser

发明人： Martin Kopp ,  Roger Sandoz ,  Mario Curcio ,  Michael Glauser

IPC分类号： C07K1/26 ,  G01N27/00

CPC分类号： G01N27/44773 ,  C07K1/26 ,  C07K1/285

摘要： A disposable electrophoresis device for separation of a complex protein sample, using two-dimensional gel electrophoresis and method thereof are disclosed. The device comprises a carrier with a first area for a first dimension gel strip and a second area for a second dimension gel, where the two areas are directly in contact with each other. The device further comprises a body made of a hard component and an elastic component layer at a variable distance from the carrier. The body further comprises at least one cavity for external actuation of at least one valve, wherein the valve is represented by the elastic component layer.

摘要翻译： 公开了使用二维凝胶电泳分离复合蛋白质样品的一次性电泳装置及其方法。 该装置包括具有用于第一尺寸凝胶带的第一区域和用于第二维度凝胶的第二区域的载体，其中两个区域彼此直接接触。 该装置还包括一个由硬质部件制成的主体和一个距离载体可变距离的弹性部件层。 该主体还包括用于至少一个阀的外部致动的至少一个空腔，其中该阀由弹性部件层表示。

公开(公告)号：US09085167B2

公开(公告)日：2015-07-21

申请号：US14499491

申请日：2014-09-29

申请人： Martin Kopp ,  Stefan Adelsperger ,  Thomas Szusdziara ,  Alexander Tobian ,  Andre Schwarzkopf ,  Murat Kader

发明人： Martin Kopp ,  Stefan Adelsperger ,  Thomas Szusdziara ,  Alexander Tobian ,  Andre Schwarzkopf ,  Murat Kader

IPC分类号： B41J2/19 ,  B41J2/18 ,  B41J2/175

CPC分类号： B41J2/19 ,  B41J2/175 ,  B41J2/17596

摘要： In an arrangement to supply ink to a print head unit having a print head, an input, and an output, a back-pressure tank having ink is provided and connected with the input of the print head unit. A degassing unit for ink and having an input and an output is also provided. A first selector valve is connected to the degassing unit input, the first selector valve in a first position establishing a connection of the degassing unit with the output of the print head unit. A second selector valve is connected to the degassing unit output, the second selector valve in a first position establishing a connection of the degassing unit with the back-pressure tank.

摘要翻译： 在向具有打印头，输入和输出的打印头单元供应墨水的设置中，提供具有墨水的背压罐并与打印头单元的输入连接。 还提供一种用于油墨并具有输入和输出的脱气单元。 第一选择阀连接到除气单元输入，第一选择阀处于第一位置，建立脱气单元与打印头单元的输出的连接。 第二选择阀连接到脱气单元输出，第二选择阀处于第一位置，建立脱气单元与背压箱的连接。

公开(公告)号：US20110127292A1

公开(公告)日：2011-06-02

申请号：US12786709

申请日：2010-05-25

申请人： Emad Sarofim ,  Martin Kopp ,  Claudio Cherubini

发明人： Emad Sarofim ,  Martin Kopp ,  Claudio Cherubini

IPC分类号： B67D7/06 ,  B01L3/02 ,  B01L9/00

CPC分类号： G01N35/1083 ,  B01L9/52 ,  B01L9/523 ,  B01L2300/0829 ,  G01N35/1065

摘要： A system for dispensing one or more fluids into wells of a target multi-well plate, a holder and a method thereof are disclosed. The system provides the holder which holds the multi-well plate in a predefined holding position, the plate having a well region provided with wells for accommodating the fluids and an edge region surrounding the well region. The holder includes: a contact area which contacts the edge region to form a sealing zone which air-tightly seals a void formed between the holder and the plate; a supporting face which supports the well region in a planar condition; and a duct which connects to a pump to generate a negative pressure in the void so as to draw the well region onto the supporting face.

摘要翻译： 公开了一种用于将一种或多种流体分配到目标多孔板的孔中的系统，保持器及其方法。 该系统提供将多孔板保持在预定的保持位置的保持器，该板具有设置有用于容纳流体的井的井区域和围绕井区域的边缘区域。 保持器包括：接触区域，其接触边缘区域以形成气密密封形成在保持器和板之间的空隙的密封区域; 在平坦状态下支撑井区的支撑面; 以及连接到泵以在空隙中产生负压以将阱区域拉到支撑面上的管道。

公开(公告)号：US20100064781A1

公开(公告)日：2010-03-18

申请号：US12556925

申请日：2009-09-10

申请人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

发明人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

IPC分类号： G01N21/00 ,  B01L3/00 ,  G01N1/44

CPC分类号： B01L3/50851 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858

摘要： Systems, methods and multi-well plates comprising an array of wells for thermal treatment of chemical or biological samples are disclosed. Each of the wells comprises a bottom opening, an upper opening, inner side walls extending from the bottom opening to the upper opening, a protrusion extending from the inner side walls into the well with a first cross-sectional area and located at a height from the bottom opening which is smaller than the height from the upper opening. A sample chamber with a second cross-sectional area is formed between the bottom opening and the protrusion. An upper chamber with a third cross-sectional area is formed between the protrusion and the upper opening, and in which the first cross-sectional area is smaller than the third cross-sectional area and smaller than or equal to the second cross-sectional area.

摘要翻译： 公开了包括用于化学或生物样品的热处理的孔阵列的系统，方法和多孔板。 每个孔包括底部开口，上部开口，从底部开口延伸到上部开口的内侧壁，从内侧壁延伸进入井的突起，其具有第一横截面积并且位于与 底部开口小于从上部开口的高度。 具有第二横截面积的样品室形成在底部开口和突起之间。 在所述突起与所述上部开口之间形成具有第三横截面积的上部腔室，其中，所述第一横截面积小于所述第三横截面积，并且小于或等于所述第二横截面积 。

公开(公告)号：US08808647B2

公开(公告)日：2014-08-19

申请号：US12556925

申请日：2009-09-10

申请人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

发明人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

IPC分类号： B01L3/00

CPC分类号： B01L3/50851 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858

摘要： Systems, methods and multi-well plates comprising an array of wells for thermal treatment of chemical or biological samples are disclosed. Each of the wells comprises a bottom opening, an upper opening, inner side walls extending from the bottom opening to the upper opening, a protrusion extending from the inner side walls into the well with a first cross-sectional area and located at a height from the bottom opening which is smaller than the height from the upper opening. A sample chamber with a second cross-sectional area is formed between the bottom opening and the protrusion. An upper chamber with a third cross-sectional area is formed between the protrusion and the upper opening, and in which the first cross-sectional area is smaller than the third cross-sectional area and smaller than or equal to the second cross-sectional area.

摘要翻译： 公开了包括用于化学或生物样品的热处理的孔阵列的系统，方法和多孔板。 每个孔包括底部开口，上部开口，从底部开口延伸到上部开口的内侧壁，从内侧壁延伸进入井的突起，其具有第一横截面积并且位于与 底部开口小于从上部开口的高度。 具有第二横截面积的样品室形成在底部开口和突起之间。 在所述突起与所述上部开口之间形成具有第三横截面积的上部腔室，其中，所述第一横截面积小于所述第三横截面积，并且小于或等于所述第二横截面积 。

公开(公告)号：US20060110763A1

公开(公告)日：2006-05-25

申请号：US11280571

申请日：2005-11-16

申请人： Martin Kopp

发明人： Martin Kopp

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/686 ,  B01L7/52

摘要： The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two step amplification method speeds up overall reaction time without affecting the limit of detection.

摘要翻译： 本发明涉及通过使样品经受热循环从模板核酸制备核酸的方法，装置和计算机程序。 在第一数量的热循环之后，部分量的反应混合物经受第二数量的热循环。 这两步扩增方法加快了整个反应时间，而不影响检测限。

公开(公告)号：US20060081600A1

公开(公告)日：2006-04-20

申请号：US11226637

申请日：2005-09-13

申请人： Martin Kopp ,  Emad Sarofim

发明人： Martin Kopp ,  Emad Sarofim

IPC分类号： F27D11/04

CPC分类号： G05D23/19 ,  B01L7/52 ,  B01L2300/1822 ,  B01L2300/1833

摘要： A method to adjust a temperature of a fluid is disclosed, the method comprising the steps of providing a heat sink taking effect on the fluid, heating the fluid by Joule heating, and adjusting the heat provided by Joule heating in order to reach a desired temperature of the fluid. Furthermore, an apparatus to carry out the method is also disclosed. Therewith, a very efficient temperature change of the fluid can very quickly be obtained since the temperature of the surrounding is not substantially changed by heat diffusion. While a temperature increase is carried out by Joule heating, a temperature decrease is carried out by simply deactivating Joule heating. As soon as Joule heating is deactivated, the heat is extracted from the fluid by the heat sink, thereby initiating a high speed temperature change towards lower temperatures.

摘要翻译： 公开了一种调节流体温度的方法，该方法包括以下步骤：提供散热器对流体的作用，通过焦耳加热加热流体，并调节由焦耳加热提供的热量以达到所需温度 的流体。 此外，还公开了一种执行该方法的装置。 因此，由于通过热扩散基本上不改变周围的温度，所以可以非常快速地获得流体的非常有效的温度变化。 当通过焦耳加热进行温度升高时，通过简单地停用焦耳加热来进行温度降低。 一旦焦耳加热被停用，热量通过散热器从流体中提取，从而开始向较低温度的高速温度变化。

公开(公告)号：US5842013A

公开(公告)日：1998-11-24

申请号：US596127

申请日：1996-02-08

申请人： Martin Kopp

发明人： Martin Kopp

IPC分类号： G06F13/10 ,  G06F9/50 ,  G06F13/42 ,  G06F15/16 ,  G06F15/177 ,  G06F15/80 ,  G06F9/00

CPC分类号： G06F15/8015

摘要： Very large amounts of data may be handled by the user interface of computers. The main processor is thus permanently overloaded, so that the computing power available for the actual application is sharply reduced in a manner that cannot be predicted without limiting the user requests. A new computer architecture is disclosed which allows a higher and more exactly predictable computing power to be achieved. In order to make the rest of the system independent from the user interface, the computer is subdivided in such a way that a first module (part 1) takes over the display and operation tasks of the user interface, a second module (part 2) takes over the processing of the application programs without their user interface, and a third module (part 3) takes over the remaining functional units. All modules have their own internal data paths. An interface between the first and second modules support the exchange of messages and data without affecting the independent processes in the first and second modules. The first and second modules have their own access paths to common devices located in the third module.

摘要翻译： PCT No.PCT / DE94 / 00848 Sec。 371日期1996年2月8日 102（e）日期1996年2月8日PCT提交1994年7月22日PCT公布。 公开号WO95 / 04973 日期2005年02月16日计算机的用户界面可能会处理大量数据。 主处理器因此永久地过载，使得可用于实际应用的计算能力以不能在不限制用户请求的情况下预测的方式急剧减少。 公开了一种新的计算机架构，其允许实现更高和更精确可预测的计算能力。 为了使系统的其余部分独立于用户界面，计算机被细分为第一模块（第1部分）接管用户界面的显示和操作任务，第二模块（第2部分） 在没有用户界面的情况下接管应用程序的处理，第三模块（第3部分）接管剩余的功能单元。 所有模块都有自己的内部数据路径。 第一和第二模块之间的接口支持消息和数据的交换，而不影响第一和第二模块中的独立进程。 第一和第二模块具有到位于第三模块中的通用设备的自己的访问路径。