公开(公告)号：US20110127292A1

公开(公告)日：2011-06-02

申请号：US12786709

申请日：2010-05-25

申请人： Emad Sarofim ,  Martin Kopp ,  Claudio Cherubini

发明人： Emad Sarofim ,  Martin Kopp ,  Claudio Cherubini

IPC分类号： B67D7/06 ,  B01L3/02 ,  B01L9/00

CPC分类号： G01N35/1083 ,  B01L9/52 ,  B01L9/523 ,  B01L2300/0829 ,  G01N35/1065

摘要： A system for dispensing one or more fluids into wells of a target multi-well plate, a holder and a method thereof are disclosed. The system provides the holder which holds the multi-well plate in a predefined holding position, the plate having a well region provided with wells for accommodating the fluids and an edge region surrounding the well region. The holder includes: a contact area which contacts the edge region to form a sealing zone which air-tightly seals a void formed between the holder and the plate; a supporting face which supports the well region in a planar condition; and a duct which connects to a pump to generate a negative pressure in the void so as to draw the well region onto the supporting face.

摘要翻译： 公开了一种用于将一种或多种流体分配到目标多孔板的孔中的系统，保持器及其方法。 该系统提供将多孔板保持在预定的保持位置的保持器，该板具有设置有用于容纳流体的井的井区域和围绕井区域的边缘区域。 保持器包括：接触区域，其接触边缘区域以形成气密密封形成在保持器和板之间的空隙的密封区域; 在平坦状态下支撑井区的支撑面; 以及连接到泵以在空隙中产生负压以将阱区域拉到支撑面上的管道。

公开(公告)号：US20100064781A1

公开(公告)日：2010-03-18

申请号：US12556925

申请日：2009-09-10

申请人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

发明人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

IPC分类号： G01N21/00 ,  B01L3/00 ,  G01N1/44

CPC分类号： B01L3/50851 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858

摘要： Systems, methods and multi-well plates comprising an array of wells for thermal treatment of chemical or biological samples are disclosed. Each of the wells comprises a bottom opening, an upper opening, inner side walls extending from the bottom opening to the upper opening, a protrusion extending from the inner side walls into the well with a first cross-sectional area and located at a height from the bottom opening which is smaller than the height from the upper opening. A sample chamber with a second cross-sectional area is formed between the bottom opening and the protrusion. An upper chamber with a third cross-sectional area is formed between the protrusion and the upper opening, and in which the first cross-sectional area is smaller than the third cross-sectional area and smaller than or equal to the second cross-sectional area.

摘要翻译： 公开了包括用于化学或生物样品的热处理的孔阵列的系统，方法和多孔板。 每个孔包括底部开口，上部开口，从底部开口延伸到上部开口的内侧壁，从内侧壁延伸进入井的突起，其具有第一横截面积并且位于与 底部开口小于从上部开口的高度。 具有第二横截面积的样品室形成在底部开口和突起之间。 在所述突起与所述上部开口之间形成具有第三横截面积的上部腔室，其中，所述第一横截面积小于所述第三横截面积，并且小于或等于所述第二横截面积 。

公开(公告)号：US08808647B2

公开(公告)日：2014-08-19

申请号：US12556925

申请日：2009-09-10

申请人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

发明人： Claudio Cherubini ,  Nicole Gwerder ,  Martin Kopp ,  Edwin Oosterbroek ,  Emad Sarofim

IPC分类号： B01L3/00

CPC分类号： B01L3/50851 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858

摘要： Systems, methods and multi-well plates comprising an array of wells for thermal treatment of chemical or biological samples are disclosed. Each of the wells comprises a bottom opening, an upper opening, inner side walls extending from the bottom opening to the upper opening, a protrusion extending from the inner side walls into the well with a first cross-sectional area and located at a height from the bottom opening which is smaller than the height from the upper opening. A sample chamber with a second cross-sectional area is formed between the bottom opening and the protrusion. An upper chamber with a third cross-sectional area is formed between the protrusion and the upper opening, and in which the first cross-sectional area is smaller than the third cross-sectional area and smaller than or equal to the second cross-sectional area.

摘要翻译： 公开了包括用于化学或生物样品的热处理的孔阵列的系统，方法和多孔板。 每个孔包括底部开口，上部开口，从底部开口延伸到上部开口的内侧壁，从内侧壁延伸进入井的突起，其具有第一横截面积并且位于与 底部开口小于从上部开口的高度。 具有第二横截面积的样品室形成在底部开口和突起之间。 在所述突起与所述上部开口之间形成具有第三横截面积的上部腔室，其中，所述第一横截面积小于所述第三横截面积，并且小于或等于所述第二横截面积 。

公开(公告)号：US20060081600A1

公开(公告)日：2006-04-20

申请号：US11226637

申请日：2005-09-13

申请人： Martin Kopp ,  Emad Sarofim

发明人： Martin Kopp ,  Emad Sarofim

IPC分类号： F27D11/04

CPC分类号： G05D23/19 ,  B01L7/52 ,  B01L2300/1822 ,  B01L2300/1833

摘要： A method to adjust a temperature of a fluid is disclosed, the method comprising the steps of providing a heat sink taking effect on the fluid, heating the fluid by Joule heating, and adjusting the heat provided by Joule heating in order to reach a desired temperature of the fluid. Furthermore, an apparatus to carry out the method is also disclosed. Therewith, a very efficient temperature change of the fluid can very quickly be obtained since the temperature of the surrounding is not substantially changed by heat diffusion. While a temperature increase is carried out by Joule heating, a temperature decrease is carried out by simply deactivating Joule heating. As soon as Joule heating is deactivated, the heat is extracted from the fluid by the heat sink, thereby initiating a high speed temperature change towards lower temperatures.

摘要翻译： 公开了一种调节流体温度的方法，该方法包括以下步骤：提供散热器对流体的作用，通过焦耳加热加热流体，并调节由焦耳加热提供的热量以达到所需温度 的流体。 此外，还公开了一种执行该方法的装置。 因此，由于通过热扩散基本上不改变周围的温度，所以可以非常快速地获得流体的非常有效的温度变化。 当通过焦耳加热进行温度升高时，通过简单地停用焦耳加热来进行温度降低。 一旦焦耳加热被停用，热量通过散热器从流体中提取，从而开始向较低温度的高速温度变化。

公开(公告)号：US08642293B2

公开(公告)日：2014-02-04

申请号：US12373517

申请日：2007-07-05

申请人： Emad Sarofim ,  Olivier Elsenhans ,  Martin Kopp ,  Hans-Peter Wahl

发明人： Emad Sarofim ,  Olivier Elsenhans ,  Martin Kopp ,  Hans-Peter Wahl

IPC分类号： C12P19/34 ,  C12M1/00 ,  C12Q1/68

CPC分类号： B01L3/502715 ,  B01L3/502707 ,  B01L2200/10 ,  B01L2300/0809

摘要： The invention is directed to a disposable sample holding and processing device (1) dimensioned for being operated in a nucleic acid amplification apparatus for analyzing a liquid sample containing a nucleic acid by a nucleic acid amplification technique. The device (1) comprises chambers (15, 16) and channels (44) designed to perform the steps of capturing, amplification and detection of the nucleic acid within the device (1), and optionally also a lysis chamber (3). The binding chamber (15) contains a solid phase (14) for immobilization of a component of the sample to be analyzed, and the amplification chamber (16) is connected to the binding chamber (15) by a fluid channel (44). The device (1) comprises a rigid body (42) and at least one channel (44), the binding chamber (15) and the amplification chamber (16) are situated on side-surfaces (50) of the body (42), each of those side-surfaces (50), on which a channel (44), the binding chamber (15) or the amplification chamber (16) is situated, is covered by at least one wall (41) and these side-surfaces (50) are substantially vertical planes when the device (1) is operated in the nucleic acid amplification apparatus.

摘要翻译： 本发明涉及一种一次性样品保持和处理装置（1），其尺寸适于在用于通过核酸扩增技术分析含有核酸的液体样品的核酸扩增装置中操作。 设备（1）包括设计成执行在装置（1）内捕获，扩增和检测核酸以及任选还有裂解室（3）的步骤的室（15,16）和通道（44）。 装订室（15）包含用于固定待分析样品的组分的固相（14），并且扩散室（16）通过流体通道（44）连接到装订室（15）。 装置（1）包括刚体（42）和至少一个通道（44），装订室（15）和放大室（16）位于主体（42）的侧表面（50）上， 其中通道（44），装订室（15）或放大室（16）所在的那些侧表面（50）中的每一个被至少一个壁（41）和这些侧表面 50）在装置（1）在核酸扩增装置中操作时是基本垂直的平面。

公开(公告)号：US20100003683A1

公开(公告)日：2010-01-07

申请号：US12373517

申请日：2007-07-05

申请人： Emad Sarofim ,  Olivier Elsenhans ,  Martin Kopp ,  Hans-Peter Wahl

发明人： Emad Sarofim ,  Olivier Elsenhans ,  Martin Kopp ,  Hans-Peter Wahl

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M1/34

CPC分类号： B01L3/502715 ,  B01L3/502707 ,  B01L2200/10 ,  B01L2300/0809

摘要： The invention is directed to a disposable sample holding and processing device (1) dimensioned for being operated in a nucleic acid amplification apparatus for analyzing a liquid sample containing a nucleic acid by a nucleic acid amplification technique. The device (1) comprises chambers (15, 16) and channels (44) designed to perform the steps of capturing, amplification and detection of the nucleic acid within the device (1), and optionally also a lysis chamber (3). The binding chamber (15) contains a solid phase (14) for immobilization of a component of the sample to be analyzed, and the amplification chamber (16) is connected to the binding chamber (15) by a fluid channel (44). The device (1) comprises a rigid body (42) and at least one channel (44), the binding chamber (15) and the amplification chamber (16) are situated on side-surfaces (50) of the body (42), each of those side-surfaces (50), on which a channel (44), the binding chamber (15) or the amplification chamber (16) is situated, is covered by at least one wall (41) and these side-surfaces (50) are substantially vertical planes when the device (1) is operated in the nucleic acid amplification apparatus.

摘要翻译： 本发明涉及一种一次性样品保持和处理装置（1），其尺寸适于在用于通过核酸扩增技术分析含有核酸的液体样品的核酸扩增装置中操作。 设备（1）包括设计成执行在装置（1）内捕获，扩增和检测核酸以及任选还有裂解室（3）的步骤的室（15,16）和通道（44）。 装订室（15）包含用于固定待分析样品的组分的固相（14），并且扩散室（16）通过流体通道（44）连接到装订室（15）。 装置（1）包括刚体（42）和至少一个通道（44），装订室（15）和放大室（16）位于主体（42）的侧表面（50）上， 其中通道（44），装订室（15）或放大室（16）所在的那些侧表面（50）中的每一个被至少一个壁（41）和这些侧表面 50）在装置（1）在核酸扩增装置中操作时是基本垂直的平面。

公开(公告)号：US20090227006A1

公开(公告)日：2009-09-10

申请号：US12373276

申请日：2007-07-05

申请人： Martin Kopp ,  Hans-Peter Wahl ,  Emad Sarofim ,  Oliver Elsenhans ,  Goran Savatic ,  Roland Christof Hutter

发明人： Martin Kopp ,  Hans-Peter Wahl ,  Emad Sarofim ,  Oliver Elsenhans ,  Goran Savatic ,  Roland Christof Hutter

IPC分类号： C12M1/00

CPC分类号： B01L3/502715 ,  G01N35/0099 ,  G01N2035/00346

摘要： The embodiments of the present invention are directed to an automatic apparatus (55), which is designed for performing nucleic acid analysis, optionally including sample preparation, with integrated disposable sample holding and processing devices (1), which devices (1) comprise a sample to be analyzed with the apparatus (55). The apparatus (55) comprises workstations (17, 40) for receiving, holding and processing such integrated disposable devices (1), a reagents station (56) for reagents (25), transfer means (36) for performing movements of an actuator, fluid actuation means for processing the sample (32) and reagents in said disposable devices (1), temperature control means (37) for heating or cooling, detection means for measuring a property of the processed samples (32), and control means for controlling the sample (32) preparation, the handling of the disposable devices (1) and the sample analysis performed by the apparatus (55).

摘要翻译： 本发明的实施例涉及一种自动装置（55），其被设计用于执行核酸分析，任选地包括样品制备，具有集成的一次性样品保持和处理装置（1），所述装置（1）包括样品 用装置（55）进行分析。 装置（55）包括用于接收，保持和处理这种集成的一次性装置（1），用于试剂（25）的试剂台（56），用于执行致动器的运动的传送装置（36） 用于处理所述一次性装置（1）中的样品（32）和试剂的流体致动装置，用于加热或冷却的温度控制装置（37），用于测量经处理样品（32）的性质的检测装置，以及用于控制 样品（32）制备，一次性装置（1）的处理和由装置（55）进行的样品分析。

公开(公告)号：US08814808B2

公开(公告)日：2014-08-26

申请号：US12879500

申请日：2010-09-10

申请人： Irio Guiseppe Calasso ,  Patrick Griss ,  Emad Sarofim ,  Rainer Jaeggi ,  Uwe Kraemer ,  Dave Hasker ,  Volker Zimmer ,  Wilifried Schmid ,  Otto Fuerst ,  Hans List ,  Hans-Peter Haar ,  Theo Arnitz ,  Steven N. Roe

发明人： Irio Guiseppe Calasso ,  Patrick Griss ,  Emad Sarofim ,  Rainer Jaeggi ,  Uwe Kraemer ,  Dave Hasker ,  Volker Zimmer ,  Wilifried Schmid ,  Otto Fuerst ,  Hans List ,  Hans-Peter Haar ,  Theo Arnitz ,  Steven N. Roe

IPC分类号： A61B5/151 ,  A61B5/15 ,  A61B5/145

CPC分类号： A61B5/1411 ,  A61B5/14532 ,  A61B5/1486 ,  A61B5/150022 ,  A61B5/150068 ,  A61B5/150282 ,  A61B5/150358 ,  A61B5/150396 ,  A61B5/150419 ,  A61B5/150427 ,  A61B5/15045 ,  A61B5/150503 ,  A61B5/15151 ,  A61B5/15163

摘要： Body fluid sampling device comprising a skin-piercing element having a collection zone for receiving body fluid. In one embodiment, the collection zone includes a plurality of holes, e.g., 3 or more holes, or 50 to 100 holes. The hole size may be small, e.g., having a diameter of 0.01 to 0.5 mm. The holes may or may not extend through the lancet. In some embodiments, the holes have a depth of 50 to 500 μm. In other embodiments, the collection zone is porous, or is a roughened area. The collection zone can take up a very small volume of body fluid, e.g., 3 to 10 nL, in a very short time period, e.g., less than 0.5 seconds. In other embodiments, the device further comprises a fluid receiving means spaced apart from the collection zone so that body fluid in the collection zone will not contact the fluid receiving means initially.

摘要翻译： 体液取样装置包括具有用于接收体液的收集区的皮肤刺穿元件。 在一个实施例中，收集区包括多个孔，例如3个或更多个孔，或50至100个孔。 孔尺寸可以较小，例如具有0.01至0.5mm的直径。 这些孔可以延伸穿过刺血针也可能不延伸穿过刺血针。 在一些实施例中，孔的深度为50至500μm。 在其它实施方案中，收集区是多孔的，或者是粗糙区域。 收集区可以在非常短的时间段（例如小于0.5秒）内占据非常小体积的体液，例如3至10nL。 在其他实施例中，该装置还包括与收集区间隔开的流体接收装置，使得收集区中的体液最初不会接触流体接收装置。

公开(公告)号：US08580567B2

公开(公告)日：2013-11-12

申请号：US12903492

申请日：2010-10-13

申请人： Emad Sarofim ,  Edwin Oosterbroek ,  Claudio Cherubini ,  Nenad Milicevic

发明人： Emad Sarofim ,  Edwin Oosterbroek ,  Claudio Cherubini ,  Nenad Milicevic

IPC分类号： G01N21/07

CPC分类号： G01N35/00029 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0677 ,  G01N21/07 ,  G01N2021/0325 ,  G01N2035/00099 ,  G01N2035/00495

摘要： A method for the automated analysis of liquid samples using at least one microfluidic structure is disclosed as well as microfluidic structures, a device having at least one of the microfluidic structures, a kit and a system including such microfluidic device. In one embodiment, the method may comprise: transferring a sample into a first fluid reservoir which is in fluid communication with a second fluid reservoir by a flow channel; spinning the microfluidic structure so as to transport the sample into one or more dead-end recesses or chambers; transferring a control fluid into the structure which generates a barrier to flow and diffusion of the sample contained in the one or more dead-end recesses or chambers; and analyzing the sample contained in the one or more dead-end recesses or chambers.

摘要翻译： 公开了使用至少一个微流体结构自动分析液体样品的方法，以及微流体结构，具有至少一个微流体结构的装置，试剂盒和包括这种微流体装置的系统。 在一个实施例中，该方法可以包括：将样品转移到通过流动通道与第二流体储存器流体连通的第一流体储存器中; 旋转微流体结构以将样品输送到一个或多个死端凹部或腔室中; 将控制流体转移到所述结构中，所述结构产生包含在所述一个或多个死端凹部或腔室中的样品的流动和扩散的阻挡层; 并分析包含在一个或多个死端凹部或腔室中的样品。

公开(公告)号：US20120295249A1

公开(公告)日：2012-11-22

申请号：US13295504

申请日：2011-11-14

申请人： Claudio Cherubini ,  Roger Iten ,  Emad Sarofim ,  Kurt Schildknecht

发明人： Claudio Cherubini ,  Roger Iten ,  Emad Sarofim ,  Kurt Schildknecht

IPC分类号： G01N21/75 ,  C12Q1/70 ,  C12Q1/06 ,  C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/50851 ,  B01L3/50853 ,  B01L7/52 ,  B01L2200/022 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1805 ,  C12Q1/686 ,  G01N21/0332 ,  G01N21/253 ,  G01N21/645 ,  G01N21/6452 ,  G01N35/028 ,  G01N2021/6419 ,  G01N2021/6484 ,  G01N2035/00346 ,  G01N2035/00376 ,  G01N2201/0627

摘要： An instrument and a method for the automated thermal treatment of liquid samples are disclosed. An inter-distance between a temperature-controlled receptacle for loading with a plurality of vessels for containing the samples and end portions of optical fibers can be varied, wherein the receptacle is configured to form a thermal communication with the loaded vessels and wherein the optical fibers have first and second end portions. The first end portion and the second end portion of each optical fiber is fixed with respect to each other for transmitting light, wherein the variation of the inter-distance allows the vessels to be loaded to or unloaded from the receptacle and to enable detection of light from the samples contained in the one or more receptacle-loaded vessels.

摘要翻译： 公开了用于液体样品的自动热处理的仪器和方法。 用于装载用于容纳样品和光纤的端部的多个容器的温度控制容器之间的距离可以变化，其中容器被配置为与加载的容器形成热连通，并且其中光纤 具有第一和第二端部。 每个光纤的第一端部和第二端部相对于彼此固定以透射光，其中间距的变化允许容器被装载或从插座卸载并且能够检测光 从包含在一个或多个容器加载的容器中的样品。