利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

17 条记录 (耗时 0.032 秒)

    Methods and devices for characterizing duplex nucleic acid molecules
    4.
    发明授权
    Methods and devices for characterizing duplex nucleic acid molecules 有权
    表征双相核酸分子的方法和装置

    公开(公告)号：US06936433B2

    公开(公告)日：2005-08-30

    申请号：US09990102

    申请日：2001-11-21

    申请人： Mark Akeson ,  Wenonah Vercoutere ,  David Haussler ,  Stephen Winters-Hilt

    发明人： Mark Akeson ,  Wenonah Vercoutere ,  David Haussler ,  Stephen Winters-Hilt

    IPC分类号： C12Q1/68 ,  G01N27/04 ,  C12Q1/37 ,  C12Q1/00 ,  G01N33/53

    CPC分类号： G01N33/48721 ,  C12Q1/6825 ,  C12Q1/6827 ,  Y10S435/975 ,  C12Q2565/631 ,  C12Q2565/607

    摘要： Methods and devices are provided for characterizing a duplex nucleic acid, e.g., a duplex DNA molecule. In the subject methods, a fluid conducting medium that includes a duplex nucleic acid molecule is contacted with a nanopore under the influence of an applied electric field and the resulting changes in current through the nanopore caused by the duplex nucleic acid molecule are monitored. The observed changes in current through the nanopore are then employed as a set of data values to characterize the duplex nucleic acid, where the set of data values may be employed in raw form or manipulated, e.g., into a current blockade profile. Also provided are nanopore devices for practicing the subject methods, where the subject nanopore devices are characterized by the presence of an algorithm which directs a processing means to employ monitored changes in current through a nanopore to characterize a duplex nucleic acid molecule responsible for the current changes. The subject methods and devices find use in a variety of applications, including, among other applications, the identification of an analyte duplex DNA molecule in a sample, the specific base sequence at a single nulceotide polymorphism (SNP), and the sequencing of duplex DNA molecules.

    摘要翻译： 提供用于表征双链体核酸例如双链体DNA分子的方法和装置。 在本发明方法中，包含双链体核酸分子的流体传导介质在施加的电场的影响下与纳米孔接触，并且监测由双链体核酸分子引起的通过纳米孔的电流的变化。 然后将观察到的通过纳米孔的电流变化用作一组数据值以表征双链体核酸，其中该组数据值可以以原始形式使用或被操纵，例如进入当前的封锁分布。 还提供了用于实施本发明方法的纳米孔装置，其中本发明的纳米孔装置的特征在于存在一种算法，其指导处理装置利用监测的通过纳米孔的电流的变化来表征负责当前变化的双链体核酸分子 。 主题方法和设备可用于各种应用，包括其他应用中样品中分析物双链DNA分子的鉴定，单核苷酸多态性（SNP）的特异性碱基序列和双链体DNA测序 分子。