公开(公告)号：US20170065695A1

公开(公告)日：2017-03-09

申请号：US15257607

申请日：2016-09-06

申请人： Attila Karsi ,  Mark L. Lawrence ,  Hossam Abdelhamed

发明人： Attila Karsi ,  Mark L. Lawrence ,  Hossam Abdelhamed

IPC分类号： A61K39/02 ,  C12N1/20

CPC分类号： A61K39/025 ,  A61K2039/522 ,  A61K2039/54 ,  A61K2039/542 ,  A61K2039/552 ,  A61K2039/57 ,  C07K14/195 ,  C12N1/36

摘要： A live attenuated Edwardsiella ictaluri bacterium lacking a viable gene encoding a functional evpB protein and a method of using the same to protect fish against infection from virulent Edwardsiella ictaluri. The methods and compositions for protecting fish against infection from virulent Edwardsiella ictaluri comprising administering to a fish a therapeutically effective amount of an attenuated Edwardsiella ictaluri bacterium lacking a viable gene encoding a functional EvpB protein. The bacterium may include an insertion and/or deletion mutation in the evpB gene. The fish include catfish, preferably catfish fingerling or a catfish fry. The composition may be delivered via immersion delivery, an injection delivery, an oral delivery, or combinations thereof.

摘要翻译： 缺乏编码功能性evpB蛋白的活基因的活的减毒爱德华肝炎细菌，以及使用该活性基因的方法来保护鱼免受毒性爱德华氏菌感染。 用于保护鱼免受毒性爱德华氏菌感染的方法和组合物，其包括向鱼施用治疗有效量的缺乏编码功能性EvpB蛋白的活的基因的减毒爱德华肝炎细菌。 细菌可能包括evpB基因中的插入和/或缺失突变。 鱼类包括鲶鱼，优选鲶鱼鱼苗或鲶鱼鱼苗。 组合物可以通过浸没递送，注射递送，口服递送或其组合递送。

公开(公告)号：US09375467B2

公开(公告)日：2016-06-28

申请号：US13940125

申请日：2013-07-11

申请人： Mark L. Lawrence ,  Attila Karsi

发明人： Mark L. Lawrence ,  Attila Karsi

IPC分类号： C12N15/10 ,  A61K39/02 ,  A61K39/00

CPC分类号： A61K39/025 ,  A61K2039/522 ,  A61K2039/552 ,  C12N15/102

摘要： A high throughput bioluminescence mutant screening procedure is disclosed. This procedure utilizes robotics, and bacterial luciferase to allow real-time monitoring of mutant viability. The procedure was used to decelop a live attenuated vaccine for a catfish against E. ictaluri, which is further claimed herein. Additionally, genes from other bacterial species are disclosed which may also be used to create vaccines.

摘要翻译： 公开了高通量生物发光突变体筛选方法。 该过程利用机器人技术和细菌荧光素酶来实时监测突变体的活力。 该方法被用于对用于鳗鱼的鲶鱼的减毒活疫苗进行破坏，这在本文进一步要求保护。 此外，公开了其它细菌物种的基因，其也可用于产生疫苗。

公开(公告)号：US20100040652A1

公开(公告)日：2010-02-18

申请号：US12490207

申请日：2009-06-23

申请人： Mark L. Lawrence ,  Attila Karsi

发明人： Mark L. Lawrence ,  Attila Karsi

IPC分类号： A61K39/02 ,  C12Q1/68 ,  C12N1/21 ,  A61K35/74 ,  A61P37/04

CPC分类号： A61K39/025 ,  A61K2039/522 ,  A61K2039/552 ,  C12N15/102

摘要： A high throughput bioluminescence mutant screening procedure is disclosed. This procedure utilizes robotics, and bacterial luciferase to allow real-time monitoring of mutant viability. The procedure was used to decelop a live attenuated vaccine for a catfish against E. ictaluri, which is further claimed herein. Additionally, genes from other bacterial species are disclosed which may also be used to create vaccines.

摘要翻译： 公开了高通量生物发光突变体筛选方法。 该过程利用机器人技术和细菌荧光素酶来实时监测突变体的活力。 该方法被用于对用于鳗鱼的鲶鱼的减毒活疫苗进行破坏，这在本文进一步要求保护。 此外，公开了其它细菌物种的基因，其也可用于产生疫苗。

公开(公告)号：US10232027B2

公开(公告)日：2019-03-19

申请号：US15257607

申请日：2016-09-06

申请人： Attila Karsi ,  Mark L. Lawrence ,  Hossam Abdelhamed

发明人： Attila Karsi ,  Mark L. Lawrence ,  Hossam Abdelhamed

IPC分类号： A61K39/02 ,  A61K49/00 ,  A61K39/00 ,  C07K14/195 ,  C12N1/36

摘要： A live attenuated Edwardsiella ictaluri bacterium lacking a viable gene encoding a functional evpB protein and a method of using the same to protect fish against infection from virulent Edwardsiella ictaluri. The methods and compositions for protecting fish against infection from virulent Edwardsiella ictaluri comprising administering to a fish a therapeutically effective amount of an attenuated Edwardsiella ictaluri bacterium lacking a viable gene encoding a functional EvpB protein. The bacterium may include an insertion and/or deletion mutation in the evpB gene. The fish include catfish, preferably catfish fingerling or a catfish fry. The composition may be delivered via immersion delivery, an injection delivery, an oral delivery, or combinations thereof.

公开(公告)号：US20130295136A1

公开(公告)日：2013-11-07

申请号：US13940125

申请日：2013-07-11

申请人： Mark L. Lawrence ,  Attila Karsi

发明人： Mark L. Lawrence ,  Attila Karsi

IPC分类号： A61K39/02

CPC分类号： A61K39/025 ,  A61K2039/522 ,  A61K2039/552 ,  C12N15/102

摘要： A high throughput bioluminescence mutant screening procedure is disclosed. This procedure utilizes robotics, and bacterial luciferase to allow real-time monitoring of mutant viability. The procedure was used to decelop a live attenuated vaccine for a catfish against E. ictaluri, which is further claimed herein. Additionally, genes from other bacterial species are disclosed which may also be used to create vaccines.

摘要翻译： 公开了高通量生物发光突变体筛选方法。 该过程利用机器人技术和细菌荧光素酶来实时监测突变体的活力。 该方法被用于对用于鳗鱼的鲶鱼的减毒活疫苗进行破坏，这在本文进一步要求保护。 此外，公开了其它细菌物种的基因，其也可用于产生疫苗。

公开(公告)号：US08507278B2

公开(公告)日：2013-08-13

申请号：US12490207

申请日：2009-06-23

申请人： Mark L. Lawrence ,  Attila Karsi

发明人： Mark L. Lawrence ,  Attila Karsi

IPC分类号： C12N15/63

CPC分类号： A61K39/025 ,  A61K2039/522 ,  A61K2039/552 ,  C12N15/102

摘要： A high throughput bioluminescence mutant screening procedure is disclosed. This procedure utilizes robotics, and bacterial luciferase to allow real-time monitoring of mutant viability. The procedure was used to develop a live attenuated vaccine for a catfish against E. ictaluri, which is further claimed herein. Additionally, genes from other bacterial species are disclosed which may also be used to create vaccines.

摘要翻译： 公开了高通量生物发光突变体筛选方法。 该过程利用机器人技术和细菌荧光素酶来实时监测突变体的活力。 该方法用于开发针对鳗鱼的鲶鱼的活减毒疫苗，其进一步要求保护。 此外，公开了其它细菌物种的基因，其也可用于产生疫苗。

公开(公告)号：US08183026B2

公开(公告)日：2012-05-22

申请号：US10541246

申请日：2004-01-14

申请人： Mark L. Lawrence ,  Daniel B. Paulsen ,  Daniel W. Scruggs

发明人： Mark L. Lawrence ,  Daniel B. Paulsen ,  Daniel W. Scruggs

IPC分类号： A01N63/00 ,  C12N1/00 ,  C12N1/12 ,  C12N1/36

CPC分类号： C12N9/1007 ,  A61K2039/522 ,  Y02A50/484

摘要： Live attenuated bacteria vaccines are provided. Also provided are methods by which such vaccines can be obtained, including: a method by which a copy of the dam gene from a pathogenic bacteria is cloned into a plasmid capable of replication in the same bacteria species such that it is overexpressed from either a lac promoter, tac promoter, araBAD promoter, trc promoter, trp promoter, T7, SP6, or T5 bacteriophage promoters, a native promoter from that species, or other appropriate promoter. The plasmid containing the dam gene is then transferred into the pathogens to cause increased expression of Dam resulting in the formation of a live attenuated bacterial vaccines. Alternative methods for producing the vaccine are also provided including altering or replacing the chromosomal promoter for the native dam gene so as to alter Dam expression or mutating or replacing the native dam gene so as to alter the expression of Dam in a pathogenic bacteria.

摘要翻译： 提供活的减毒细菌疫苗。 还提供了可以获得这样的疫苗的方法，包括：将来自病原菌的dam基因的拷贝克隆到能够在相同细菌种中复制的质粒的方法，使得其从lac 启动子，tac启动子，araBAD启动子，trc启动子，trp启动子，T7，SP6或T5噬菌体启动子，来自该物种的天然启动子或其他合适的启动子。 然后将含有该基因的质粒转移到病原体中以导致Dam的表达增加，导致减毒细菌疫苗的形成。 还提供了用于生产疫苗的替代方法，包括改变或替代天然阻断基因的染色体启动子，以便改变大坝表达或突变或替代天然阻断基因，从而改变病原菌中的表达。

公开(公告)号：US07368547B2

公开(公告)日：2008-05-06

申请号：US10767441

申请日：2004-01-30

申请人： Mark L. Lawrence ,  Dongyou Liu ,  A. Jerald Ainsworth ,  Frank W. Austin

发明人： Mark L. Lawrence ,  Dongyou Liu ,  A. Jerald Ainsworth ,  Frank W. Austin

IPC分类号： C07H21/02 ,  C07H21/00 ,  C07H21/04 ,  C12P21/04 ,  C12N1/20 ,  C12N15/00 ,  C12N15/63

CPC分类号： C12Q1/689 ,  C07H21/04 ,  C12Q2600/16

摘要： A method for identifying virulent strains of L. monocytogenes that includes the use of primers or probes in a PCR assay or hybridization technique that employs primers or probes, which are specific for virulence-specific genes of L. monocytogenes. Also provided is a method of control of L. monocytogenes strains that have been identified using the method of the present invention.

摘要翻译： 一种用于鉴定单核细胞增生李斯特菌的毒性菌株的方法，其包括在PCR测定中使用引物或探针或使用引物或探针的杂交技术，所述引物或探针对单核细胞增生李斯特菌的毒力特异性基因是特异性的。 还提供了使用本发明的方法鉴定的单核细胞增生李斯特氏菌菌株的控制方法。