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公开(公告)号：US5196317A
公开(公告)日：1993-03-23
申请号：US608443
申请日：1990-11-02
IPC分类号： C12N9/00 , C12N1/21 , C12N9/04 , C12N15/09 , C12N15/53 , C12N15/67 , C12N15/73 , C12P21/00 , C12P21/02 , C12R1/19
CPC分类号： C12N9/0006 , C12N15/67 , C12N15/73
摘要： The invention relates to a process for achieving high, thermally-induced expression rates, which last for a relatively long period of time, of proteins in recombinant cells, preferably in E. coli cells, by optimizing cell density, induction temperature and supplemented amino-acid-containing substrates.
摘要翻译： 本发明涉及通过优化细胞密度，诱导温度和补充的氨基酸序列，实现重组细胞中，优选在大肠杆菌细胞中蛋白质持续相当长的时间的高的热诱导表达率的方法， 含酸底物。
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公开(公告)号：US3964974A
公开(公告)日：1976-06-22
申请号：US395547
申请日：1973-09-10
申请人： Dieter Banauch , Wolfgang Brummer , Wolfgang Ebeling , Roland Helger , Norbert Hennrich , Hermann Lang
发明人： Dieter Banauch , Wolfgang Brummer , Wolfgang Ebeling , Roland Helger , Norbert Hennrich , Hermann Lang
CPC分类号： C12Q1/54
摘要： Glucose is rapidly ad quantitatively determined with an analytical agent containing glucose dehydrogenase having an activity of at least 2 .mu./mg and NADH.sub.2 oxidase activity less than 0.1%, a pyridine coenzyme, a buffer and mutarotase to increase spontaneous mutarotation of alpha-glucose to beta-glucose. There may be optionally present inhibitors for reduced pyridine coenzyme oxidases and a thermal stabilizing amount of alkali metal chloride.
摘要翻译： 使用含有至少2μM/ mg活性和NADH2氧化酶活性小于0.1％的葡萄糖脱氢酶，吡啶辅酶，缓冲液和变性酶的分析试剂快速定量测定葡萄糖，以增加α-葡萄糖自发变性至β -葡萄糖。 可以任选地存在还原吡啶辅酶氧化酶和热稳定量的碱金属氯化物的抑制剂。
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公开(公告)号：US4800161A
公开(公告)日：1989-01-24
申请号：US772979
申请日：1985-09-05
CPC分类号： C12Q1/54 , C12N9/90 , Y10S435/814 , Y10S435/822
摘要： The invention relates to a process for the microbiological preparation of aldose-1-epimerase by cultivating microorganisms in a nutrient medium and release of the enzyme from the cells, which process is characterized in that microorganisms of the genus Acinetobacter are used.
摘要翻译： 本发明涉及通过在营养培养基中培养微生物并从细胞中释放酶来微生物制备醛糖-1-差向异构酶的方法，该方法的特征在于使用不动杆菌属的微生物。
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4.发明授权Drilling machine for drilling large drilling holes in rock, particularly underground 失效用于在岩石，特别是地下钻大钻孔的钻孔机
公开(公告)号：US5029655A
公开(公告)日：1991-07-09
申请号：US437456
申请日：1989-11-15
申请人： Wolfgang Ebeling
发明人： Wolfgang Ebeling
CPC分类号： E21B4/006 , E21B3/02 , E21B4/16 , Y10T74/19056
摘要： A drilling machine for drilling large drill holes in rock, especially underground, comprises a support, a rotary drilling drive longitudinally displaceable on the support in a drilling direction and provided with a reducing transmission and drive motors. The reducing transmission including a planetary transmission which is transmissively and selectively connectable with two of the drive motors as a superposed transmission.
摘要翻译： 用于在岩石，特别是地下钻大钻孔的钻孔机包括支撑件，在钻孔方向上在支撑件上纵向移位的旋转钻孔驱动器，并具有减速传动和驱动马达。 减速传动装置包括行星传动装置，该行星传动装置作为叠加的传动装置可与两个驱动电动机进行透射和选择性连接。
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5.发明授权Process for the preparation of glucose dehydrogenase from Bacillus megaterium 失效从BACILLUS MEGATERIUM制备葡萄糖脱氢酶的方法
公开(公告)号：US5126256A
公开(公告)日：1992-06-30
申请号：US179359
申请日：1988-04-08
IPC分类号： C12N1/20 , C12N1/21 , C12N9/04 , C12N15/00 , C12N15/09 , C12N15/53 , C12Q1/32 , C12R1/11 , C12R1/19
CPC分类号： C12N9/0006
摘要： A process for the preparation of glucose dehydrogenase from Bacillus megaterium. This invention relates to the preparation of a genetically engineered polypeptide with the biological activity of the enzyme glucose dehydrogenase, and a new DNA sequence, recombinant DNA vector, and transformed microorganism therefor.
摘要翻译： 从巨大芽孢杆菌制备葡萄糖脱氢酶的方法。 本发明涉及具有酶葡萄糖脱氢酶生物活性的遗传工程多肽的制备，以及新的DNA序列，重组DNA载体及其转化的微生物。
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公开(公告)号：US4705120A
公开(公告)日：1987-11-10
申请号：US903118
申请日：1986-09-02
申请人： Wolfgang Ebeling , Helmut Kolditz
发明人： Wolfgang Ebeling , Helmut Kolditz
摘要： A drilling machine for drilling descending large boreholes in earth or rocktructures, comprises an elongated drill pipe driven by a drive machine positioned outside a borehole being drilled, a drill head connected to said pipe and a support device for a drill head. A fluid-supplying device having a descending conduit, supplying fluid from the fluid supplying device to the drill head, is further provided in the drilling machine. A sluice charged with the mixture of the material drilled and the fluid is arranged in the drill head and driven by the drillpipe. The support device is secured in the machine against rotation.
摘要翻译： 一种用于在地球或岩石结构中下沉大钻孔的钻孔钻机，包括由位于被钻孔的外部的驱动机驱动的细长钻杆，连接到所述管的钻头和用于钻头的支撑装置。 具有下降管道的流体供应装置还设置在钻孔机中，该下游导管将流体从流体供应装置供应到钻头。 装载钻井液和流体的混合物的闸门布置在钻头中并由钻杆驱动。 支撑装置固定在机器中以避免旋转。
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7.发明授权
公开(公告)号：US5250415A
公开(公告)日：1993-10-05
申请号：US873146
申请日：1992-04-24
CPC分类号： C12N9/0006
摘要： A process for the preparation of glucose dehydrogenase from Bacillus megaterium. This invention relates to the preparation of a genetically engineered polypeptide with the biological activity of the enzyme glucose dehydrogenase and a new DNA sequence, recombinant DNA vector, and transformed microrganism therefor.
摘要翻译： 从巨大芽孢杆菌制备葡萄糖脱氢酶的方法。 本发明涉及具有酶葡萄糖脱氢酶的生物活性的遗传工程改造的多肽的制备和新的DNA序列，重组DNA载体及其转化的微生物。
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公开(公告)号：US4892161A
公开(公告)日：1990-01-09
申请号：US286385
申请日：1988-12-19
申请人： Wolfgang Ebeling
发明人： Wolfgang Ebeling
IPC分类号： E21B17/046 , E21B19/20 , E21B19/24 , F01N13/18
CPC分类号： F01N13/1816 , E21B17/046 , E21B19/20 , E21B19/24 , Y10T403/592 , Y10T403/604
摘要： A rotary rock drilling machine comprises a carriage having two ends, a rotary drilling drive arranged longitudinally displaceable on the carriage, a guiding head arranged on one end of the carriage, the guiding head and the rotary drilling drive defining a longitudinal axis of the machine, a drilling rod composed of a plurality of drilling rod parts which are releasably connected with one another, the drilling rod parts having a plurality of screw convolutions, the drilling rod parts having one end having a coupling pin with a snap pin and another end having a coupling bush with an engaging opening for the snap pin, a positioning device in the guiding head arranged to arrest a rotary position of the drilling rod, and an unlocking device for the snap pin, the positioning device including a locking bar which extends substantially parallel to the longitudinal axis and displaceable radially to the drilling rod, and which is insertable in at least one radial slot provided in the screw convolution of the drilling rod.
摘要翻译： 旋转式凿岩机包括具有两端的滑架，在滑架上纵向移动的旋转钻孔驱动装置，布置在滑架一端的导向头，导向头和旋转钻孔驱动器限定机器的纵向轴线， 由多个可释放地彼此连接的钻杆部件组成的钻杆，所述钻杆部件具有多个螺旋旋转，所述钻杆部件具有一个具有卡扣销的联接销，另一端具有 连接衬套，其具有用于卡扣销的接合开口;布置成阻止钻杆的旋转位置的引导头中的定位装置，以及用于卡销的解锁装置，所述定位装置包括基本上平行于 纵向轴线并且可径向移动到钻杆，并且其可插入设置在螺杆convo中的至少一个径向槽中 凿钻杆。
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公开(公告)号：US4877094A
公开(公告)日：1989-10-31
申请号：US202872
申请日：1988-06-03
申请人： Wolfgang Ebeling
发明人： Wolfgang Ebeling
CPC分类号： E21B3/02 , E21B19/16 , Y10T279/1241
摘要： A rock drilling machine, particularly for underground excavations comprises a drilling carriage, and a rotary drilling drive arranged in a longitudinally displaceable manner on the drilling carriage, the rotary drilling drive having a drive shaft, an adaptor connected with the drive shaft for joint rotation therewith and having a threaded end for form-locking receipt of drilling rods with a respective counter thread, and a clamping head co-rotating with the drive shaft for force-transmitting receipt of outwardly smooth drilling rods, the adaptor being arranged forwardly of the clamping head as considered in a drilling direction, the threaded end of the adaptor extending outwardly beyond the clamping head for free observation and being releasably mounted on a part of the drive shaft independently of the clamping head.
摘要翻译： 一种特别用于地下挖掘的凿岩机包括一个钻孔支架和一个以纵向可移动的方式布置在钻机上的旋转式钻井驱动装置，该旋转钻井驱动装置具有一个驱动轴，一个与该驱动轴连接的适配器，用于与其连接地旋转 并且具有螺纹端，用于形成锁定接收具有相应的反向螺纹的钻杆，以及与驱动轴同向旋转的夹紧头，用于向外平滑的钻杆的力传递接收，适配器布置在夹紧头的前方 如在钻井方向上所考虑的那样，适配器的螺纹端向外延伸超过夹紧头，用于自由观察，并且可独立于夹紧头可释放地安装在驱动轴的一部分上。
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公开(公告)号：US4732226A
公开(公告)日：1988-03-22
申请号：US026525
申请日：1987-03-16
申请人： Wolfgang Ebeling , Helmut Kolditz
发明人： Wolfgang Ebeling , Helmut Kolditz
摘要： A shaft drilling machine comprises a drill head carrying drill tools thereon, a device for guiding the drill head in a shaft, a device for advancing the drill head in the shaft, and drive aggregates which are lowered into the shaft together with the drill head and driving the latter. A sluice for providing a rinsing fluid is arranged in a pilot drill. A rinsing conduit and an ascending conduit for extracting from the shaft a drilled material-rinsing fluid mixture generated during the drilling operation are connected to the sluice which is formed as a bucket wheel.
摘要翻译： 一种钻井机包括在其上承载钻具的钻头，用于将钻头引导在轴中的装置，用于将钻头推进到轴中的装置以及与钻头一起下降到轴中的驱动集合体;以及 推动后者。 用于提供冲洗液的闸门布置在导向钻中。 冲洗管道和上升管道，用于从钻井中提取在钻井操作期间产生的钻井材料冲洗流体混合物连接到形成为斗轮的闸门。
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