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1.发明申请Method and Means for Enrichment, Removal and Detection of Gram-Positive Bacteria 有权富集，去除和检测革兰氏阳性细菌的方法和手段公开(公告)号：US20100092968A1
公开(公告)日：2010-04-15
申请号：US12520731
申请日：2007-12-21
CPC分类号： C12N9/88 , C07K14/005 , C07K14/195 , C07K14/32 , C07K2319/035 , C07K2319/70 , C12N2795/10022 , G01N33/56911 , G01N2469/10
摘要： The present invention relates to polypeptides comprising a enzymatically non-active cell wall binding domain of an endolysin or another cell wall lysing enzyme, and a sequence according to SEQ ID NO: 1 or derivatives thereof, wherein the polypeptide besides the cell wall binding domain comprises no further domains of an endolysin, as well as means for their preparation. The present invention further relates to methods for binding, enriching, removing from a sample, capturing and/or detecting bacteria, particularly gram positive bacteria.
摘要翻译： 本发明涉及包含内溶素或另一细胞壁裂解酶的酶促非活性细胞壁结合结构域的多肽，以及根据SEQ ID NO：1或其衍生物的序列，其中所述多肽除了细胞壁结合结构域包括 没有其他的内溶素域，以及它们的制备方法。 本发明还涉及用于结合，富集，从样品中除去，捕获和/或检测细菌，特别是革兰氏阳性细菌的方法。
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2.发明授权Method and means for enrichment, removal and detection of gram-positive bacteria 有权富集，去除和检测革兰氏阳性菌的方法和手段公开(公告)号：US09394534B2
公开(公告)日：2016-07-19
申请号：US12520731
申请日：2007-12-21
IPC分类号： C12Q1/68 , C12N9/00 , G01N33/554 , G01N33/569 , C12N9/96 , C12N1/20 , C12N15/00 , C07H21/04 , C12N9/88 , C07K14/005 , C07K14/195 , C07K14/32
CPC分类号： C12N9/88 , C07K14/005 , C07K14/195 , C07K14/32 , C07K2319/035 , C07K2319/70 , C12N2795/10022 , G01N33/56911 , G01N2469/10
摘要： The present invention relates to polypeptides comprising a enzymatically non-active cell wall binding domain of an endolysin or another cell wall lysing enzyme, and a sequence according to SEQ ID NO: 1 or derivatives thereof, wherein the polypeptide besides the cell wall binding domain comprises no further domains of an endolysin, as well as means for their preparation. The present invention further relates to methods for binding, enriching, removing from a sample, capturing and/or detecting bacteria, particularly gram positive bacteria.
摘要翻译： 本发明涉及包含内溶素或另一细胞壁裂解酶的酶促非活性细胞壁结合结构域的多肽，以及根据SEQ ID NO：1或其衍生物的序列，其中所述多肽除了细胞壁结合结构域包括 没有其他的内溶素域，以及它们的制备方法。 本发明还涉及用于结合，富集，从样品中除去，捕获和/或检测细菌，特别是革兰氏阳性细菌的方法。
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公开(公告)号：US20110243916A1
公开(公告)日：2011-10-06
申请号：US12992224
申请日：2009-05-14
CPC分类号： C12N9/503 , A61K38/00 , C12N2795/10111 , G01N33/56911
摘要： The present invention relates to polypeptides with a changed amino acid sequence on at least one amino acid position compared to the amino acid sequence according to SEQ ID NO: 1. The present invention further relates to the nucleotide sequences encoding the polypeptide, vectors, comprising the nucleotide sequences and host cells for expression of the polypeptide. The present invention further relates to the use of the polypeptides as a human, veterinary medical or diagnostic substance, in food, in cosmetics, as disinfectant or in the environmental field.
摘要翻译： 本发明涉及与根据SEQ ID NO：1的氨基酸序列相比在至少一个氨基酸位置具有改变的氨基酸序列的多肽。本发明还涉及编码多肽的核苷酸序列，载体包含 核苷酸序列和用于表达多肽的宿主细胞。 本发明还涉及多肽在食品，化妆品中作为消毒剂或在环境领域中作为人类，兽医学或诊断物质的用途。
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