公开(公告)号：US08071558B2

公开(公告)日：2011-12-06

申请号：US11909493

申请日：2006-03-22

申请人： Masaaki Tokuda ,  Ken Izumori ,  Tatsuhiro Matsuo ,  Kenji Morimoto ,  Kaoru Takekawa

发明人： Masaaki Tokuda ,  Ken Izumori ,  Tatsuhiro Matsuo ,  Kenji Morimoto ,  Kaoru Takekawa

IPC分类号： A61K31/7004 ,  A61P3/08

CPC分类号： A23K50/40 ,  A23K20/163 ,  A23L27/30 ,  A23L33/10 ,  A61K31/7004

摘要： A novel use of D-psicose (for suppressing the abnormal intra-day increase of blood glucose level) is provided. A composition containing D-psicose as the active component and for use in suppressing the abnormal intra-day increase of plasma glucose concentration. The composition is a composition in blend with D-psicose and/or a derivative thereof. The composition is in a form selected from sweeteners, seasonings, food additives, food materials, food and drink products, health food and drink products, pharmaceutical products, and feeds and blended with D-psicose, and/or a derivative thereof as the active component, for use in preventing and therapeutically treating diseases requiring the saving of insufficient insulin, the amelioration of insulin sensitivity and the amelioration of hyperglycemia, and/or diseases of which the symptoms can be ameliorated via the suppression of the abnormal intra-day increase of plasma glucose concentration or of which the onset can be prevented via the suppression thereof, such as diabetes mellitus, occult diabetic states, obesity, hyperglycemia, and/or arteriosclerosis. A method for using D-psicose in suppressing the abnormal intra-day increase of plasma glucose concentration, comprising giving D-psicose at given times to suppress the abnormal increase of plasma glucose concentration throughout the day.

摘要翻译： 提供了一种新的D-psicose的用途（用于抑制日内异常增加血糖水平）。 作为活性成分含有D-灵芝蛋白的组合物，用于抑制日内异常增加血浆葡萄糖浓度。 组合物是与D-灵芝蛋白和/或其衍生物共混的组合物。 该组合物为选自甜味剂，调味料，食品添加剂，食品材料，食品和饮料产品，保健食品和饮料产品，药品和饲料中的形式，并且与D-灵芝蛋白和/或其衍生物作为活性成分 组分，用于预防和治疗需要节省胰岛素不足的疾病，改善胰岛素敏感性和改善高血糖症，和/或通过抑制日内异常升高可以改善症状的疾病 血浆葡萄糖浓度或通过其抑制如糖尿病，隐匿性糖尿病状态，肥胖症，高血糖症和/或动脉硬化可以预防发作。 一种用于抑制血浆葡萄糖浓度异常日增加的方法，包括在给定时间给予D-psicose以抑制全天血浆葡萄糖浓度的异常增加。

公开(公告)号：US20090062215A1

公开(公告)日：2009-03-05

申请号：US11909493

申请日：2006-03-22

申请人： Masaaki Tokuda ,  Ken Izumori ,  Tatsuhiro Matsuo ,  Kenji Morimoto ,  Kaoru Takekawa

发明人： Masaaki Tokuda ,  Ken Izumori ,  Tatsuhiro Matsuo ,  Kenji Morimoto ,  Kaoru Takekawa

IPC分类号： A61K31/7004 ,  C07H1/00 ,  A61P3/08

CPC分类号： A23K50/40 ,  A23K20/163 ,  A23L27/30 ,  A23L33/10 ,  A61K31/7004

摘要： A novel use of D-psicose (for suppressing the abnormal intra-day increase of blood glucose level) is provided. A composition containing D-psicose as the active component and for use in suppressing the abnormal intra-day increase of plasma glucose concentration. The composition is a composition in blend with D-psicose and/or a derivative thereof. The composition is in a form selected from sweeteners, seasonings, food additives, food materials, food and drink products, health food and drink products, pharmaceutical products, and feeds and blended with D-psicose, and/or a derivative thereof as the active component, for use in preventing and therapeutically treating diseases requiring the saving of insufficient insulin, the amelioration of insulin sensitivity and the amelioration of hyperglycemia, and/or diseases of which the symptoms can be ameliorated via the suppression of the abnormal intra-day increase of plasma glucose concentration or of which the onset can be prevented via the suppression thereof, such as diabetes mellitus, occult diabetic states, obesity, hyperglycemia, and/or arteriosclerosis. A method for using D-psicose in suppressing the abnormal intra-day increase of plasma glucose concentration, comprising giving D-psicose at given times to suppress the abnormal increase of plasma glucose concentration throughout the day.

摘要翻译： 提供了一种新的D-psicose的用途（用于抑制日内异常增加血糖水平）。 作为活性成分含有D-灵芝蛋白的组合物，用于抑制日内异常增加血浆葡萄糖浓度。 组合物是与D-灵芝蛋白和/或其衍生物共混的组合物。 该组合物为选自甜味剂，调味料，食品添加剂，食品材料，食品和饮料产品，保健食品和饮料产品，药品和饲料中的形式，并且与D-灵芝蛋白和/或其衍生物作为活性成分 组分，用于预防和治疗需要节省胰岛素不足的疾病，改善胰岛素敏感性和改善高血糖症，和/或通过抑制日内异常升高可以改善症状的疾病 血浆葡萄糖浓度或通过其抑制如糖尿病，隐匿性糖尿病状态，肥胖症，高血糖症和/或动脉硬化可预防发作。 一种用于抑制血浆葡萄糖浓度异常日增加的方法，包括在给定时间给予D-psicose以抑制全天血浆葡萄糖浓度的异常增加。

公开(公告)号：US20090325231A1

公开(公告)日：2009-12-31

申请号：US12515596

申请日：2007-03-26

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12P21/00 ,  C12N9/90 ,  C12N1/21 ,  C12P19/24

CPC分类号： C12N9/90 ,  C12P19/24 ,  C12R1/01

摘要： Object: To provide a thermostable L-ribose isomerase.Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

摘要翻译： 目的：提供热稳定的L-核糖异构酶。 解决方法：具有MW的热稳定的L-核糖异构酶。 32,000（通过SDS-PAGE），最适温度为45℃，pH 9.0的最佳pH（甘氨酸 - NaOH缓冲液），以及稳定的物理化学性质，例如在pH 9.0的热处理期间温度稳定性高达45℃，为10 分钟，并且具有异构化L-核糖以产生L-核酮糖的作用或者与L-核酮糖异构化以产生L-核糖的作用。 醛糖和酮糖之间的转化方法，包括使热稳定的L-核糖异构酶作为源自（1）鸟氨酸解脂耶氏杆菌菌株MB426（NITE BP-277）的酶与选自L-核糖，D-赖索糖， D-山梨糖醇，D-甘露糖，L-丙氨酸和L-古洛糖醇以使醛糖异构化以产生选自单独对应的L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵芝蛋白和L- 山梨糖或与选自L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵敏糖和L-山梨糖的酮糖相互作用以使酮糖异构化以产生选自相应的L-核糖，D D-果糖，D-甘露糖，D-甘露糖，L-阿洛糖和L-古洛糖。

公开(公告)号：US08530222B2

公开(公告)日：2013-09-10

申请号：US12515602

申请日：2007-03-27

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12N1/20

CPC分类号： C12R1/01 ,  A61K8/60 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0006 ,  C12P19/02

摘要： Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase.Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

摘要翻译： 问题：提供具有生产脱氧多元醇脱氢酶能力的微生物。 解决方法：属于肠杆菌属的微生物，其在位置C2和C3处产生与核糖醇或L-阿托糖醇相同结构的脱氧多羟基化脱氢酶的能力。 肠杆菌属细菌细胞IK7（登录号NITE P-271）。 一种生产脱氧酮糖酮的方法，包括使含有通过培养本发明的微生物获得的脱氧多元醇脱氢酶的培养物或使脱氧多元醇脱氢酶与位置C2和C3处于相同结构的含有脱氧多羟基化合物的溶液反应，作为 核糖醇或L-天冬氨酸氧化脱氧多元醇以产生相应的脱氧酮糖，然后收集脱氧酮糖酮。 脱氧多元醇是1-脱氧-D-多糖，而相应的脱氧酮基是1-脱氧-L-精氨酸。 否则，脱氧多元醇为L-麦芽糖醇，而相应的脱氧酮糖为1-脱氧-L-果糖。

公开(公告)号：US08227232B2

公开(公告)日：2012-07-24

申请号：US12515596

申请日：2007-03-26

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12N9/90 ,  C12N1/00 ,  C12P19/24 ,  C07H1/00 ,  C07H1/06

CPC分类号： C12N9/90 ,  C12P19/24 ,  C12R1/01

摘要： Object: To provide a thermostable L-ribose isomerase.Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

摘要翻译： 目的：提供热稳定的L-核糖异构酶。 解决方法：具有MW的热稳定性L-核糖异构酶。 32,000（通过SDS-PAGE），最适温度为45℃，pH 9.0的最佳pH（甘氨酸 - NaOH缓冲液），以及稳定的物理化学性质，例如在pH 9.0的热处理期间温度稳定性高达45℃，为10 分钟，并且具有异构化L-核糖以产生L-核酮糖的作用或者与L-核酮糖异构化以产生L-核糖的作用。 醛糖和酮糖之间的转化方法，包括使热稳定的L-核糖异构酶作为源自（1）鸟氨酸解脂耶氏杆菌菌株MB426（NITE BP-277）的酶与选自L-核糖，D-赖索糖， D-山梨糖醇，D-甘露糖，L-丙氨酸和L-古洛糖醇以使醛糖异构化以产生选自单独对应的L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵芝蛋白和L- 山梨糖或与选自L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵敏糖和L-山梨糖的酮糖相互作用以使酮糖异构化以产生选自相应的L-核糖，D D-果糖，D-甘露糖，D-甘露糖，L-阿洛糖和L-古洛糖。

公开(公告)号：US20100167350A1

公开(公告)日：2010-07-01

申请号：US12515602

申请日：2007-03-27

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12P19/00 ,  C07H3/08 ,  C12N1/20

CPC分类号： C12R1/01 ,  A61K8/60 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0006 ,  C12P19/02

摘要： Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase.Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

摘要翻译： 问题：提供具有生产脱氧多元醇脱氢酶能力的微生物。 解决方法：属于肠杆菌属的微生物，其在位置C2和C3处产生与核糖醇或L-阿托糖醇相同结构的脱氧多羟基化脱氢酶的能力。 肠杆菌属细菌细胞IK7（登录号NITE P-271）。 一种生产脱氧酮糖酮的方法，包括使含有通过培养本发明的微生物获得的脱氧多元醇脱氢酶的培养物或使脱氧多元醇脱氢酶与位置C2和C3处于相同结构的含有脱氧多羟基化合物的溶液反应，作为 核糖醇或L-天冬氨酸氧化脱氧多元醇以产生相应的脱氧酮糖，然后收集脱氧酮糖酮。 脱氧多元醇是1-脱氧-D-多糖，而相应的脱氧酮基是1-脱氧-L-精氨酸。 否则，脱氧多元醇为L-麦芽糖醇，而相应的脱氧酮糖为1-脱氧-L-果糖。

公开(公告)号：US08748589B2

公开(公告)日：2014-06-10

申请号：US11817774

申请日：2006-03-03

申请人： Ken Izumori ,  Masaaki Tokuda ,  Goro Takata ,  Kenji Morimoto

发明人： Ken Izumori ,  Masaaki Tokuda ,  Goro Takata ,  Kenji Morimoto

IPC分类号： C07H1/00

CPC分类号： C12P19/02 ,  C12P19/24

摘要： A complex crystalline sugar comprising D-psicose and D-allose and a method for producing the same are disclosed. The compositional ratio between D-psicose and D-allose in the sugar is about 1:1 to 1:4. A process for producing a complex crystalline sugar comprising D-psicose and D-allose, the process comprising producing a complex crystalline sugar comprising D-psicose and D-allose from a sugar solution containing D-psicose and p-allose and collecting the complex crystalline sugar. The solvent of the sugar solution used in the production of the complex crystalline sugar is water or a mixture of water and ethanol. The sugar solution containing D-psicose and D-allose is produced by a process comprising reacting D-psicose with L-rhamnose isomerase to convert D-psicose into D-allose. The L-rhamnose isomerase is derived from a strain (IPOD FERM BP-08593) belonging to Pseudomonas stutzeri.

摘要翻译： 公开了一种复合结晶糖，其包含D-木糖和D-阿洛糖及其制备方法。 糖中D-灵糖和D-阿洛糖的组成比为约1:1至1:4。 一种制备复合结晶糖的方法，该复合结晶糖包括D-木糖和D-阿洛糖，该方法包括由含有D-木糖和对 - 阿洛糖的糖溶液制备包含D-灵敏蛋白和D-阿洛糖的复合结晶糖并收集复合结晶糖 糖。 用于生产复合结晶糖的糖溶液的溶剂是水或水和乙醇的混合物。 通过包括使D-灵芝糖与L-鼠李糖异构酶反应以将D-灵芝蛋白转化为D-丙氨酸的方法产生含有D-灵敏蛋白和D-阿洛糖的糖溶液。 L-鼠李糖异构酶衍生自属于假单胞菌（Strepomonas stutzeri）的菌株（IPOD FERM BP-08593）。

公开(公告)号：US20090068710A1

公开(公告)日：2009-03-12

申请号：US11817774

申请日：2006-03-03

申请人： Ken Izumori ,  Masaaki Tokuda ,  Goro Takata ,  Kenji Morimoto

发明人： Ken Izumori ,  Masaaki Tokuda ,  Goro Takata ,  Kenji Morimoto

IPC分类号： C12P19/24 ,  C07H1/00

CPC分类号： C12P19/02 ,  C12P19/24

摘要： Disclosed is a process for producing a crystalline sugar comprising D-psicose and D-allose. Also disclosed is a process for producing the sugar.A complex crystalline sugar comprising D-psicose and D-allose. The compositional ratio between D-psicose and D-allose in the sugar is about 1:1 to 1:4. A process for producing a complex crystalline sugar comprising D-psicose and D-allose, the process comprising producing a complex crystalline sugar comprising D-psicose and D-allose from a sugar solution containing D-psicose and p-allose and collecting the complex crystalline sugar. The solvent of the sugar solution used in the production of the complex crystalline sugar is water or a mixture of water and ethanol. The sugar solution containing D-psicose and D-allose is produced by a process comprising reacting D-psicose with L-rhamnose isomerase to convert D-psicose into D-allose. The L-rhamnose isomerase is derived from a strain (IPOD FERM BP-08593) belonging to Pseudomonas stutzeri.

摘要翻译： 公开了一种生产包含D-木糖和D-阿洛糖的结晶糖的方法。 还公开了生产糖的方法。 一种复合结晶糖，其包含D-木糖和D-丙氨酸。 糖中D-灵糖和D-阿洛糖的组成比为约1:1至1:4。 一种制备复合结晶糖的方法，该复合结晶糖包括D-木糖和D-阿洛糖，该方法包括由含有D-木糖和对 - 阿洛糖的糖溶液制备包含D-灵敏蛋白和D-阿洛糖的复合结晶糖并收集复合结晶糖 糖。 用于生产复合结晶糖的糖溶液的溶剂是水或水和乙醇的混合物。 通过包括使D-灵芝糖与L-鼠李糖异构酶反应以将D-灵芝蛋白转化为D-丙氨酸的方法产生含有D-灵敏蛋白和D-阿洛糖的糖溶液。 L-鼠李糖异构酶衍生自属于假单胞菌（Strepomonas stutzeri）的菌株（IPOD FERM BP-08593）。

公开(公告)号：US08440619B2

公开(公告)日：2013-05-14

申请号：US12065452

申请日：2006-08-31

申请人： Masaaki Tokuda ,  Shoji Kimura ,  Ken Izumori

发明人： Masaaki Tokuda ,  Shoji Kimura ,  Ken Izumori

IPC分类号： A61K38/00 ,  A61K9/12 ,  A61K31/70 ,  A61P9/00

CPC分类号： A23K20/163 ,  A23L33/10 ,  A61K31/7004

摘要： The object is to provide a novel application of D-allose (e.g., use for prevention of hypertension or hypercardia). Thus, disclosed is a composition for preventing the increase in blood pressure which comprises D-allose as an active ingredient; or a composition containing D-allose and/or a derivative thereof, preferably in an amount of 0.1 to 50% by weight. Preferably, the composition is in a form selected from the group consisting of a food additive, a food material, a beverage/food, a health beverage/food, a pharmaceutical and a feeding stuff which can be used for the prevention and treatment of a cardiovascular system disorder (e.g., hypertension, hypercardia). The increase in blood pressure may be caused by salt-sensitive hypertension. Also disclosed is use of D-allose for preventing the increase in blood pressure (excluding medical practices).

摘要翻译： 本发明的目的是提供D-丙氨酸的新应用（例如，用于预防高血压或高血压）。 因此，公开了用于防止血压升高的组合物，其包含D-阿洛糖作为活性成分; 或含有D-丙氨酸和/或其衍生物的组合物，优选为0.1〜50重量％。 优选地，组合物为选自食品添加剂，食品材料，饮料/食品，保健饮料/食品，药物和饲料的形式，其可用于预防和治疗 心血管系统疾病（如高血压，高血压）。 血压升高可能是盐敏感性高血压引起的。 还公开了使用D-丙氨酸来防止血压升高（不包括医疗实践）。

公开(公告)号：US07501267B2

公开(公告)日：2009-03-10

申请号：US11714887

申请日：2007-03-07

申请人： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

发明人： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N1/21 ,  C12N9/90

CPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01014

摘要： In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

摘要翻译： 在采食稀少糖的策略（图1）中，旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系，因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质（a）或（b）的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO：2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法，其特征在于，在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞，从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖，其特征在于，在单糖的总体图中，目标稀有糖的位置被理解，因此设计了允许上述蛋白质起作用的最佳生产途径。