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    METHOD OF DETECTING METHYLATED CYTOSINE
    2.
    发明申请
    METHOD OF DETECTING METHYLATED CYTOSINE 有权
    检测甲基化胞嘧啶的方法

    公开(公告)号：US20100240042A1

    公开(公告)日：2010-09-23

    申请号：US12674648

    申请日：2008-08-20

    申请人： Masahiro Kajita ,  Noriko Oka ,  Noriaki Yamamoto ,  Ayako Sakai ,  Hideki Ishihara

    发明人： Masahiro Kajita ,  Noriko Oka ,  Noriaki Yamamoto ,  Ayako Sakai ,  Hideki Ishihara

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12M1/34

    CPC分类号： C12Q1/6827 ,  C12Q2537/164 ,  C12Q2525/119 ,  C12Q2523/115

    摘要： The method for detecting a methylated cytosine of the present invention comprises the steps of:hybridizing a sample DNA with an oligonucleotide which can hybridize with a region of the sample DNA containing a cytosine suspected of being methylated and has an a basic site at the position complementary to the cytosine;reacting the hybridized sample DNA obtained in the previous step with an oxidizing agent to oxidize the cytosine when it is methylated; anddetecting the oxidized methylated cytosine.

    摘要翻译： 检测本发明的甲基化胞嘧啶的方法包括以下步骤：将样品DNA与能够与疑似被甲基化的胞嘧啶的样品DNA的区域杂交的寡核苷酸杂交，并且在互补位置具有基本位点 到胞嘧啶; 使上一步获得的杂交样品DNA与氧化剂反应，以在甲基化时氧化胞嘧啶; 并检测氧化的甲基化胞嘧啶。

    Method for confirming conversion treatment and nucleic acid molecule used therefor
    5.
    发明申请
    Method for confirming conversion treatment and nucleic acid molecule used therefor 审中-公开
    用于确认转化处理的方法和用于其的核酸分子

    公开(公告)号：US20080096213A1

    公开(公告)日：2008-04-24

    申请号：US11902254

    申请日：2007-09-20

    申请人： Masahiro Kajita ,  Noriaki Yamamoto ,  Ayako Sakai ,  Hideki Ishihara

    发明人： Masahiro Kajita ,  Noriaki Yamamoto ,  Ayako Sakai ,  Hideki Ishihara

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6827 ,  C12Q2565/1015 ,  C12Q2545/114 ,  C12Q2545/101

    摘要： There is provided a method for confirming conversion treatment of non-methylated cytosine, including a step of mixing a DNA taken from a living body, with a nucleic acid for accuracy control having a sequence A which is not contained in a genome of the living body and contains cytosine, a step of converting non-methylated cytosine in the mixture into a base other than cytosine, a step of detecting the sequence A and/or a sequence A′ which has a sequence obtained by converting cytosine in the sequence A into the base other than cytosine, and a step of determining whether the conversion step has been properly performed or not based on the result of the detection step.

    摘要翻译： 提供了一种确认非甲基化胞嘧啶的转化处理的方法，包括将从活体取得的DNA混合的步骤与用于精确控制的核酸的步骤，所述序列A不包含在生物体的基因组中 并含有胞嘧啶，将混合物中的非甲基化胞嘧啶转化为除胞嘧啶以外的碱基的步骤，检测序列A和/或A序列的步骤，其具有通过将序列A中的胞嘧啶转化为 碱基以外的碱基，以及基于检测步骤的结果确定转化步骤是否已经被适当地进行的步骤。

    Kit and method for determining whether or not unmethylated cytosine conversion treatment is properly carried out and method for analyzing methylated DNA using the same
    6.
    发明授权
    Kit and method for determining whether or not unmethylated cytosine conversion treatment is properly carried out and method for analyzing methylated DNA using the same 有权
    用于确定是否正确进行非甲基化胞嘧啶转化处理的试剂盒和方法以及使用其分析甲基化DNA的方法

    公开(公告)号：US08906623B2

    公开(公告)日：2014-12-09

    申请号：US12935846

    申请日：2009-03-16

    申请人： Kaya Tai ,  Ayako Sakai ,  Noriaki Yamamoto ,  Noriko Oka ,  Masahiro Kajita

    发明人： Kaya Tai ,  Ayako Sakai ,  Noriaki Yamamoto ,  Noriko Oka ,  Masahiro Kajita

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  C12Q2523/125

    摘要： Success or failure of unmethylated cytosine conversion treatment is determined by using a first primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence not containing cytosine in the nucleotide sequence of biological DNA that is subject to an unmethylated cytosine conversion treatment and a second primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence in which cytosine in a nucleotide sequence containing cytosine and not containing a CpG site is converted into a base other than cytosine, in the nucleotide sequence of biological DNA.

    摘要翻译： 通过使用包含多个引物的第一引物组来确定未甲基化胞嘧啶转化处理的成功或失败，所述多个引物与包含未经甲基化胞嘧啶转化处理的生物DNA的核苷酸序列中不含胞嘧啶的核苷酸序列的核酸杂交， 第二引物组，其包含与包含核苷酸序列的核酸杂交的多个引物，所述核酸序列在生物DNA的核苷酸序列中将含有胞嘧啶并且不含有CpG位点的核苷酸序列中的胞嘧啶转化为除胞嘧啶以外的碱基。

    KIT AND METHOD FOR DETERMINING WHETHER OR NOT UNMETHYLATED CYTOSINE CONVERSION TREATMENT IS PROPERLY CARRIED OUT AND METHOD FOR ANALYZING METHYLATED DNA USING THE SAME
    7.
    发明申请
    KIT AND METHOD FOR DETERMINING WHETHER OR NOT UNMETHYLATED CYTOSINE CONVERSION TREATMENT IS PROPERLY CARRIED OUT AND METHOD FOR ANALYZING METHYLATED DNA USING THE SAME 有权
    用于确定是否合成非甲基化胞嘧啶转化酶处理的试剂盒和方法以及使用其分析甲基化DNA的方法

    公开(公告)号：US20110027799A1

    公开(公告)日：2011-02-03

    申请号：US12935846

    申请日：2009-03-16

    申请人： Kaya Tai ,  Ayako Sakai ,  Noriaki Yamamoto ,  Noriko Oka ,  Masahiro Kajita

    发明人： Kaya Tai ,  Ayako Sakai ,  Noriaki Yamamoto ,  Noriko Oka ,  Masahiro Kajita

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2523/125

    摘要： Success or failure of unmethylated cytosine conversion treatment is determined by using a first primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence not containing cytosine in the nucleotide sequence of biological DNA that is subject to an unmethylated cytosine conversion treatment and a second primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence in which cytosine in a nucleotide sequence containing cytosine and not containing a CpG site is converted into a base other than cytosine, in the nucleotide sequence of biological DNA.

    摘要翻译： 通过使用包含多个引物的第一引物组来确定未甲基化胞嘧啶转化处理的成功或失败，所述多个引物与包含未经甲基化胞嘧啶转化处理的生物DNA的核苷酸序列中不含胞嘧啶的核苷酸序列的核酸杂交， 第二引物组，其包含与包含核苷酸序列的核酸杂交的多个引物，所述核酸序列在生物DNA的核苷酸序列中将含有胞嘧啶并且不含有CpG位点的核苷酸序列中的胞嘧啶转化为除胞嘧啶以外的碱基。

    MICROCHIP SOLUTION SENDING SYSTEM
    9.
    发明申请
    MICROCHIP SOLUTION SENDING SYSTEM 有权
    MICROCHIP解决方案发送系统

    公开(公告)号：US20140099236A1

    公开(公告)日：2014-04-10

    申请号：US14116219

    申请日：2012-05-08

    申请人： Noriaki Yamamoto ,  Youichi Aoki

    发明人： Noriaki Yamamoto ,  Youichi Aoki

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54373 ,  B01L3/502715 ,  B01L2200/0605 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N2035/106

    摘要： A microchip solution sending system may include a flow passage assembly that is at least provided with a fine flow passage that is provided with a detection region including a formed reaction field to which an antibody that reacts with a specific antigen is fixed; and a micro pump that is connected to the flow passage assembly and that is configured to send a specimen material solution that includes the specific antigen in a reciprocating manner. The specimen material solution that has been sent may pass through the detection region in a repetitive manner in the case in which the micro pump sends the specimen material solution in a reciprocating manner,

    摘要翻译： 微芯片溶液传送系统可以包括至少具有细流路的流动通道组件，该流动通道设置有检测区域，该检测区域包括与特定抗原反应的抗体固定的形成的反应场; 以及微型泵，其与所述流路组件连接并且被配置为以往复方式发送包括所述特定抗原的样品材料溶液。 在微型泵以往复方式发送样品材料溶液的情况下，已经送出的样品材料溶液可以重复地通过检测区域，