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    Method of degrading plastic and process for producing useful substance using the same
    1.
    发明申请
    Method of degrading plastic and process for producing useful substance using the same 审中-公开
    降解塑料的方法和使用其制造有用物质的方法

    公开(公告)号：US20060106120A1

    公开(公告)日：2006-05-18

    申请号：US10532423

    申请日：2003-09-17

    申请人： Keietsu Abe ,  Katsuya Gomi ,  Yohei Yamagata ,  Fumihiko Hasegawa ,  Hiroshi Maeda ,  Tasuku Nakajima ,  Masayuki Machida

    发明人： Keietsu Abe ,  Katsuya Gomi ,  Yohei Yamagata ,  Fumihiko Hasegawa ,  Hiroshi Maeda ,  Tasuku Nakajima ,  Masayuki Machida

    IPC分类号： C08J11/04

    CPC分类号： C12N9/242 ,  B29B17/00 ,  C07K14/375 ,  C08J11/105 ,  C08J2367/02 ,  C08J2367/04 ,  C12N9/18 ,  Y02W30/62 ,  Y02W30/702

    摘要： A method of degrading a plastic in the presence of a biosurfactant; a method of degrading a plastic by contacting the plastic with a microorganism; a process for producing a useful substance from a plastic which comprises degrading the plastic by contacting the plastic with a microorganism and further converting the components of the thus degraded plastic with the use of a microorganism; a method of degrading a plastic by contacting the plastic with a microorganism in the coexistence of a biosurfactant and/or a plastic-degrading enzyme and thus degrading the plastic under the action of the microorganism; a transformant microorganism having been recombined with at least one DNA selected from among a DNA containing a gene encoding a surface active substance, a DNA containing a gene encoding a plastic-degrading enzyme and a DNA containing a gene encoding a useful substance; novel genes as described above; and proteins encoded thereby.

    摘要翻译： 在生物表面活性剂存在下降解塑料的方法; 通过使塑料与微生物接触来降解塑料的方法; 一种从塑料制造有用物质的方法，包括通过使塑料与微生物接触来降解塑料，并且通过使用微生物进一步转化由此降解的塑料的组分; 通过在生物表面活性剂和/或塑性降解酶的共存下使塑料与微生物接触从而在微生物的作用下降解塑料来降解塑料的方法; 已经与选自包含编码表面活性物质的DNA的DNA，含有编码塑性降解酶的基因的DNA和含有编码有用物质的基因的DNA重组的转化体微生物; 如上所述的新基因; 和由此编码的蛋白质。

    OxlT sequence and its use
    10.
    发明授权
    OxlT sequence and its use 失效
    OxIT序列及其用途

    公开(公告)号：US6162632A

    公开(公告)日：2000-12-19

    申请号：US30267

    申请日：1998-02-25

    申请人： Peter C. Maloney ,  Keietsu Abe ,  Zhong-Shi Ruan

    发明人： Peter C. Maloney ,  Keietsu Abe ,  Zhong-Shi Ruan

    IPC分类号： C07K14/195 ,  C12N1/20

    CPC分类号： C07K14/195

    摘要： A DNA sequence encoding a transport protein responsible for the physical movement of oxalate and formate across the plasma membrane is provided. The protein, OxlT, can be isolated from the gram negative anaerobe, Oxalobacter formigenes. The protein is part of the three protein oxalate/formate antiport exchange system that allows for conversion of extracellular oxalate to formate+CO.sub.2.The OxlT coding sequence can be used to provide a metabolic energy system to the cell. The system comprises the OxlT transport protein in combination with oxalylCoA decarboxylase and formyl-CoA transferase. Expression cassettes can be designed to express all or part of the system in a target host. Such energy generating systems can be used in industrial settings that utilize fermentation. The energy generating systems of the invention extend culture longevity and increase product yield.In another aspect of the invention, the OxlT coding sequence can be used in pharmaceutical compositions for manipulating serum oxalic acid levels in patients in need thereof. In this manner, liposomes comprising the energy generating system can be developed and administered to humans and other animals.

    摘要翻译： 提供编码负责草酸盐和甲酸盐穿过质膜的物理运动的转运蛋白的DNA序列。 蛋白质OxlT可以从革兰氏阴性厌氧菌（Oxalobacter formigenes）中分离出来。 蛋白质是三种蛋白质草酸盐/甲酸盐逆转录酶交换体系的一部分，允许将细胞外草酸转化为甲酸+二氧化碳。 OxlT编码序列可用于向细胞提供代谢能系统。 该系统包含OxlT转运蛋白与草酰CoA脱羧酶和甲酰辅酶A转移酶的组合。 表达盒可以设计成在目标主机中表达系统的全部或部分。 这种能量产生系统可用于利用发酵的工业环境中。 本发明的能量产生系统延长了养殖寿命，提高了产量。 在本发明的另一方面，OxlT编码序列可用于在有需要的患者中操纵血清草酸水平的药物组合物中。 以这种方式，可以开发包含能量产生系统的脂质体并将其施用于人和其他动物。