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    Method of producing a nucleic acid encoding an antibody
    2.
    发明授权
    Method of producing a nucleic acid encoding an antibody 失效
    生产编码抗体的核酸的方法

    公开(公告)号：US08008076B2

    公开(公告)日：2011-08-30

    申请号：US11587701

    申请日：2005-04-27

    申请人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fujii ,  Miho Watanabe ,  Koichi Matsubara ,  Yu Jau Chen ,  Juliana Sim

    发明人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fujii ,  Miho Watanabe ,  Koichi Matsubara ,  Yu Jau Chen ,  Juliana Sim

    IPC分类号： C12N15/63 ,  C12N5/06 ,  C12N1/20 ,  C07H21/04

    CPC分类号： A01K67/027 ,  A01K2267/01 ,  A61K35/16 ,  C07K16/00 ,  C07K16/30

    摘要： An objective of the present invention is to facilitate the acquisition of antibody-producing cells that are infiltrating virus-infected cells, cancer cells, abnormal cells forming a benign hyperplasia, and the like, and to improve the efficiency of the production of antibodies as well as nucleic acids encoding them from the antibody-producing cells.The present inventors discovered that, when cancer tissues comprising infiltrating lymphocytes are transplanted into highly immunodeficient animals that do not have T cells, B cells, and NK cells and further exhibit a low IFN production ability, the differentiation and proliferation of infiltrating lymphocytes are unexpectedly promoted, and the number of plasma cells that produce antibodies recognizing cancer tissues increases dramatically, plasma cells can be separated easily, and antibodies or nucleic acids encoding them can be easily prepared from the plasma cells.

    摘要翻译： 本发明的目的是便于获得渗透病毒感染细胞的抗体产生细胞，癌细胞，形成良性增生的异常细胞等，以及提高抗体生产的效率 作为从抗体产生细胞编码它们的核酸。 本发明人发现，当将包含浸润性淋巴细胞的癌组织移植到不具有T细胞，B细胞和NK细胞的高度免疫缺陷的动物中并且进一步表现出低的IFN产生能力时，意外地促进了浸润性淋巴细胞的分化和增殖 并且产生识别癌组织的抗体的浆细胞的数量急剧增加，可以容易地分离浆细胞，并且可以容易地从浆细胞制备抗体或编码它们的核酸。

    Method of Proliferating Plasma Cells
    3.
    发明申请
    Method of Proliferating Plasma Cells 失效
    增殖血浆细胞的方法

    公开(公告)号：US20080187537A1

    公开(公告)日：2008-08-07

    申请号：US11587701

    申请日：2005-04-27

    申请人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fujii ,  Miho Watanabe ,  Koichi Matsubara ,  Yu Jau Chen ,  Juliana Sim

    发明人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fujii ,  Miho Watanabe ,  Koichi Matsubara ,  Yu Jau Chen ,  Juliana Sim

    IPC分类号： A61K39/395 ,  A61K35/12 ,  C12P21/04 ,  C07H21/00 ,  A61P43/00 ,  C07K16/18 ,  A61K31/70 ,  G01N33/574

    CPC分类号： A01K67/027 ,  A01K2267/01 ,  A61K35/16 ,  C07K16/00 ,  C07K16/30

    摘要： An objective of the present invention is to facilitate the acquisition of antibody-producing cells that are infiltrating virus-infected cells, cancer cells, abnormal cells forming a benign hyperplasia, and the like, and to improve the efficiency of the production of antibodies as well as nucleic acids encoding them from the antibody-producing cells.The present inventors discovered that, when cancer tissues comprising infiltrating lymphocytes are transplanted into highly immunodeficient animals that do not have T cells, B cells, and NK cells and further exhibit a low IFN production ability, the differentiation and proliferation of infiltrating lymphocytes are unexpectedly promoted, and the number of plasma cells that produce antibodies recognizing cancer tissues increases dramatically, plasma cells can be separated easily, and antibodies or nucleic acids encoding them can be easily prepared from the plasma cells.

    摘要翻译： 本发明的目的是便于获得渗透病毒感染细胞的抗体产生细胞，癌细胞，形成良性增生的异常细胞等，以及提高抗体生产的效率 作为从抗体产生细胞编码它们的核酸。 本发明人发现，当将包含浸润淋巴细胞的癌组织移植到不具有T细胞，B细胞和NK细胞的高度免疫缺陷的动物中并且进一步表现出低的IFN产生能力时，意外地促进了浸润性淋巴细胞的分化和增殖 并且产生识别癌组织的抗体的浆细胞的数量急剧增加，可以容易地分离浆细胞，并且可以容易地从浆细胞制备抗体或编码它们的核酸。

    Novel gene having reverse transcriptas motif
    4.
    发明申请
    Novel gene having reverse transcriptas motif 审中-公开
    具有逆转录本基序的新基因

    公开(公告)号：US20050272092A1

    公开(公告)日：2005-12-08

    申请号：US11199124

    申请日：2005-08-09

    申请人： Masayuki Tsuchiya ,  Kenji Yoshida

    发明人： Masayuki Tsuchiya ,  Kenji Yoshida

    IPC分类号： C07H21/04 ,  C12N9/12 ,  C12N9/22 ,  C12N15/12 ,  C12Q1/68

    CPC分类号： C12N9/1276 ,  C12Y207/07049

    摘要： The present invention aims to provide a novel gene having a reverse transcriptase motif. The invention isolates a novel gene having a reverse transcriptase motif, and gives its complete base sequence determined. The invention also provides a protein encoded by the gene, and an antibody against the protein. The use of them is useful in developing a method for detecting telomerase activity, a method for detecting a cancer cell, a telomerase activity inhibitor, and a method for screening a telomerase activity inhibitor.

    摘要翻译： 本发明旨在提供具有逆转录酶基序的新基因。 本发明分离具有逆转录酶基序的新基因，并确定其完整的碱基序列。 本发明还提供了由该基因编码的蛋白质和针对该蛋白质的抗体。 其使用可用于开发端粒酶活性检测方法，癌细胞检测方法，端粒酶活性抑制剂及筛选端粒酶活性抑制剂的方法。

    Method of Proliferating Plasma Cells
    5.
    发明申请
    Method of Proliferating Plasma Cells 失效
    增殖血浆细胞的方法

    公开(公告)号：US20120015402A1

    公开(公告)日：2012-01-19

    申请号：US13187677

    申请日：2011-07-21

    申请人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fujii ,  Miho Watanabe ,  Koichi Matsubara ,  Yu Jau Chen ,  Juliana Sim

    发明人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fujii ,  Miho Watanabe ,  Koichi Matsubara ,  Yu Jau Chen ,  Juliana Sim

    IPC分类号： C12P21/00 ,  C12N5/071

    CPC分类号： A01K67/027 ,  A01K2267/01 ,  A61K35/16 ,  C07K16/00 ,  C07K16/30

    摘要： An objective of the present invention is to facilitate the acquisition of antibody-producing cells that are infiltrating virus-infected cells, cancer cells, abnormal cells forming a benign hyperplasia, and the like, and to improve the efficiency of the production of antibodies as well as nucleic acids encoding them from the antibody-producing cells.The present inventors discovered that, when cancer tissues comprising infiltrating lymphocytes are transplanted into highly immunodeficient animals that do not have T cells, B cells, and NK cells and further exhibit a low IFN production ability, the differentiation and proliferation of infiltrating lymphocytes are unexpectedly promoted, and the number of plasma cells that produce antibodies recognizing cancer tissues increases dramatically, plasma cells can be separated easily, and antibodies or nucleic acids encoding them can be easily prepared from the plasma cells.

    摘要翻译： 本发明的目的是便于获得渗透病毒感染细胞的抗体产生细胞，癌细胞，形成良性增生的异常细胞等，以及提高抗体生产的效率 作为从抗体产生细胞编码它们的核酸。 本发明人发现，当将包含浸润性淋巴细胞的癌组织移植到不具有T细胞，B细胞和NK细胞的高度免疫缺陷的动物中并且进一步表现出低的IFN产生能力时，意外地促进了浸润性淋巴细胞的分化和增殖 并且产生识别癌组织的抗体的浆细胞的数量急剧增加，可以容易地分离浆细胞，并且可以容易地从浆细胞制备抗体或编码它们的核酸。

    Methods of generating and using antibody-producing cells
    6.
    发明授权
    Methods of generating and using antibody-producing cells 失效
    产生和使用抗体产生细胞的方法

    公开(公告)号：US08343761B2

    公开(公告)日：2013-01-01

    申请号：US13187677

    申请日：2011-07-21

    申请人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fujii ,  Miho Watanabe ,  Koichi Matsubara ,  Yu Jau Chen ,  Juliana Sim

    发明人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fujii ,  Miho Watanabe ,  Koichi Matsubara ,  Yu Jau Chen ,  Juliana Sim

    IPC分类号： C12N5/06 ,  A01K67/00 ,  C12N5/00

    CPC分类号： A01K67/027 ,  A01K2267/01 ,  A61K35/16 ,  C07K16/00 ,  C07K16/30

    摘要： An objective of the present invention is to facilitate the acquisition of antibody-producing cells that are infiltrating virus-infected cells, cancer cells, abnormal cells forming a benign hyperplasia, and the like, and to improve the efficiency of the production of antibodies as well as nucleic acids encoding them from the antibody-producing cells.The present inventors discovered that, when cancer tissues comprising infiltrating lymphocytes are transplanted into highly immunodeficient animals that do not have T cells, B cells, and NK cells and further exhibit a low IFN production ability, the differentiation and proliferation of infiltrating lymphocytes are unexpectedly promoted, and the number of plasma cells that produce antibodies recognizing cancer tissues increases dramatically, plasma cells can be separated easily, and antibodies or nucleic acids encoding them can be easily prepared from the plasma cells.

    摘要翻译： 本发明的目的是便于获得渗透病毒感染细胞的抗体产生细胞，癌细胞，形成良性增生的异常细胞等，以及提高抗体生产的效率 作为从抗体产生细胞编码它们的核酸。 本发明人发现，当将包含浸润性淋巴细胞的癌组织移植到不具有T细胞，B细胞和NK细胞的高度免疫缺陷的动物中并且进一步表现出低的IFN产生能力时，意外地促进了浸润性淋巴细胞的分化和增殖 并且产生识别癌组织的抗体的浆细胞的数量急剧增加，可以容易地分离浆细胞，并且可以容易地从浆细胞制备抗体或编码它们的核酸。

    Antibodies against lesion tissue
    7.
    发明申请
    Antibodies against lesion tissue 审中-公开
    针对病变组织的抗体

    公开(公告)号：US20060235207A1

    公开(公告)日：2006-10-19

    申请号：US10535764

    申请日：2003-11-21

    申请人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fuji ,  Kouichi Matsubara ,  Hiroyuki Tsunoda

    发明人： Masayuki Tsuchiya ,  Masami Suzuki ,  Kenji Yoshida ,  Etsuko Fuji ,  Kouichi Matsubara ,  Hiroyuki Tsunoda

    IPC分类号： C07K16/30 ,  G01N33/574 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06

    CPC分类号： C07K16/00 ,  C07K16/30 ,  C07K16/3015 ,  C07K2317/21 ,  C07K2317/622

    摘要： Methods for isolating polynucleotides encoding antibodies against lesional tissues are provided, wherein the methods comprise the steps of: (a) isolating a B cell(s) that infiltrates into a lesional tissue of interest; and (b) obtaining an antibody-encoding polynucleotide from the isolated B cell(s). The lesions may be a cancer tissue or such. Antibody genes can be obtained without depending on B cell cloning. Accordingly, it is also possible to obtain genes encoding human-derived antibodies which are difficult to clone. Genes that encode antibodies against cancer can be obtained using cancer tissues as the lesion.

    摘要翻译： 提供了分离编码针对损伤组织的抗体的多核苷酸的方法，其中所述方法包括以下步骤：（a）分离渗透到感兴趣的损伤组织中的B细胞; 和（b）从分离的B细胞获得抗体编码多核苷酸。 病变可以是癌组织等。 可以获得抗体基因而不依赖于B细胞克隆。 因此，也可以获得难以克隆的编码人源性抗体的基因。 编码针对癌症的抗体的基因可以使用癌组织作为病变获得。

    CRT-1 gene having reverse transcriptase motif
    8.
    发明授权
    CRT-1 gene having reverse transcriptase motif 失效
    具有逆转录酶基序的CRT-1基因

    公开(公告)号：US06608188B1

    公开(公告)日：2003-08-19

    申请号：US09582924

    申请日：2000-07-07

    申请人： Masayuki Tsuchiya ,  Kenji Yoshida

    发明人： Masayuki Tsuchiya ,  Kenji Yoshida

    IPC分类号： C12Q168

    CPC分类号： C12N9/1276 ,  C12Y207/07049

    摘要： The present invention aims to provide a novel gene having a reverse transcriptase motif. The invention isolates a novel gene having a reverse transcriptase motif, and gives its complete base sequence determined. The invention also provides a protein encoded by the gene, and an antibody against the protein. The use of them is useful in developing a method for detecting telomerase activity, a method for detecting a cancer cell, a telomerase activity inhibitor, and a method for screening a telomerase activity inhibitor.

    摘要翻译： 本发明旨在提供具有逆转录酶基序的新基因。 本发明分离具有逆转录酶基序的新基因，并确定其完整的碱基序列。 本发明还提供了由该基因编码的蛋白质和针对该蛋白质的抗体。 它们的用途可用于开发检测端粒酶活性的方法，检测癌细胞的方法，端粒酶活性抑制剂和筛选端粒酶活性抑制剂的方法。

    Proteins having a reverse transcriptase motif
    10.
    发明授权
    Proteins having a reverse transcriptase motif 失效
    具有逆转录酶基序的蛋白质

    公开(公告)号：US06949373B2

    公开(公告)日：2005-09-27

    申请号：US10294778

    申请日：2002-11-15

    申请人： Masayuki Tsuchiya ,  Kenji Yoshida

    发明人： Masayuki Tsuchiya ,  Kenji Yoshida

    IPC分类号： C07H21/04 ,  C12N9/12 ,  C12N9/22 ,  C12N15/12 ,  C12Q1/68 ,  C07H21/02 ,  C07K1/00 ,  C07K14/00

    CPC分类号： C12N9/1276 ,  C12Y207/07049

    摘要： The present invention aims to provide a novel gene having a reverse transcriptase motif. The invention isolates a novel gene having a reverse transcriptase motif, and gives its complete base sequence determined. The invention also provides a protein encoded by the gene, and an antibody against the protein. The use of them is useful in developing a method for detecting telomerase activity, a method for detecting a cancer cell, a telomerase activity inhibitor, and a method for screening a telomerase activity inhibitor.

    摘要翻译： 本发明旨在提供具有逆转录酶基序的新基因。 本发明分离具有逆转录酶基序的新基因，并确定其完整的碱基序列。 本发明还提供了由该基因编码的蛋白质和针对该蛋白质的抗体。 它们的用途可用于开发检测端粒酶活性的方法，检测癌细胞的方法，端粒酶活性抑制剂和筛选端粒酶活性抑制剂的方法。