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1.发明申请Lactic Acid-Producing Yeast Cells Having Nonfunctional L- or D-Lactate:Ferricytochrome C Oxidoreductase Cells 有权具有非功能性L-或D-乳酸的乳酸生产酵母细胞：铁染色质C氧化还原酶细胞公开(公告)号：US20090253189A1
公开(公告)日：2009-10-08
申请号：US12093757
申请日：2006-11-17
申请人： Matthew Miller , Pirkko Suominen , Aristos Aristidou , Benjamin Matthew Hause , Pim Van Hoek , Catherine Asleson Dundon
发明人： Matthew Miller , Pirkko Suominen , Aristos Aristidou , Benjamin Matthew Hause , Pim Van Hoek , Catherine Asleson Dundon
CPC分类号： C12N9/0006 , C12P7/56 , C12Y101/02003
摘要： Yeast cells having an exogenous lactate dehydrogenase gene ae modified by reducing L- or D-lactate:ferricytochrome c oxidoreductase activity in the cell. This leads to reduced consumption of lactate by the cell and can increase overall lactate yields in a fermentation process. Cells having the reduced I, or D-lactate:ferricytochrome c oxidoreductase activity can be screened for by resistance to organic acids such as lactic or glycolic acid.
摘要翻译： 酵母细胞具有通过减少细胞中的L-或D-乳酸：铁染色体氧化还原酶活性而修饰的外源性乳酸脱氢酶基因。 这导致细胞对乳酸盐的消耗降低，并且可以增加发酵过程中的总体乳酸盐产量。 具有降低的I或D-乳酸：铁染色质c氧化还原酶活性的细胞可通过耐有机酸如乳酸或乙醇酸进行筛选。
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2.发明授权Lactic acid-producing yeast cells having nonfunctional L- or D-lactate:ferricytochrome C oxidoreductase cells 有权具有非功能性L-或D-乳酸的产酸酵母细胞：铁染色质C氧化还原酶细胞公开(公告)号：US08137953B2
公开(公告)日：2012-03-20
申请号：US12093757
申请日：2006-11-17
申请人： Matthew Miller , Pirkko Suominen , Aristos Aristidou , Benjamin Matthew Hause , Pim Van Hoek , Catherine Asleson Dundon
发明人： Matthew Miller , Pirkko Suominen , Aristos Aristidou , Benjamin Matthew Hause , Pim Van Hoek , Catherine Asleson Dundon
CPC分类号： C12N9/0006 , C12P7/56 , C12Y101/02003
摘要： Yeast cells having an exogenous lactate dehydrogenase gene ae modified by reducing L- or D-lactate:ferricytochrome c oxidoreductase activity in the cell. This leads to reduced consumption of lactate by the cell and can increase overall lactate yields in a fermentation process. Cells having the reduced L- or D-lactate:ferricytochrome c oxidoreductase activity can be screened for by resistance to organic acids such as lactic or glycolic acid.
摘要翻译： 酵母细胞具有通过减少细胞中的L-或D-乳酸：铁染色体氧化还原酶活性而修饰的外源性乳酸脱氢酶基因。 这导致细胞对乳酸盐的消耗降低，并且可以增加发酵过程中的总体乳酸盐产量。 具有降低的L-乳酸或D-乳酸：细胞色素C氧化还原酶活性的细胞可通过耐有机酸如乳酸或乙醇酸进行筛选。
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3.发明申请YEAST CELLS HAVING DISRUPTED PATHWAY FROM DIHYDROXYACETONE PHOSPHATE TO GLYCEROL 审中-公开具有从二羟基乙酸酯磷酸盐到甘油的破坏途径的YEAST细胞公开(公告)号：US20090053782A1
公开(公告)日：2009-02-26
申请号：US12281286
申请日：2007-03-13
申请人： Catherine Asleson Dundon , Pirkko Suominen , Aristos Aristidou , Brian J. Rush , Kari Koivuranta , Benjamin Matthew Hause , Thomas William McMullin , Kevin Roberg-Perez
发明人： Catherine Asleson Dundon , Pirkko Suominen , Aristos Aristidou , Brian J. Rush , Kari Koivuranta , Benjamin Matthew Hause , Thomas William McMullin , Kevin Roberg-Perez
CPC分类号： B65G15/12 , B65G17/12 , C12N15/52 , C12P7/06 , C12P7/40 , C12P7/56 , E01B29/32 , Y02E50/17 , Y10T137/85986
摘要： Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyueromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.
摘要翻译： 酵母细胞被遗传修饰以破坏从二羟基丙酮到甘油的天然代谢途径。 在某些方面，酵母细胞是克鲁维酵母属，假丝酵母属或发chen类。 在其它方面，酵母细胞能够产生至少一种有机酸，如乳酸盐。 酵母细胞比野生型菌株产生明显较少的甘油，并且通常产生更高产量的所需发酵产物。 本发明的酵母细胞在培养时经常生长良好，尽管它们的甘油产量减少。
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公开(公告)号：US20120028323A1
公开(公告)日：2012-02-02
申请号：US13267771
申请日：2011-10-06
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/88 , C12N9/90 , C12N15/81 , C12Y101/01001 , C12Y101/01008 , C12Y101/01086 , C12Y101/05003 , C12Y202/01006 , C12Y401/01001 , C12Y401/01074 , C12Y402/01009 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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5.发明申请Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids 审中-公开酵母微生物减少副产物积累，以改善燃料，化学品和氨基酸的生产公开(公告)号：US20110201090A1
公开(公告)日：2011-08-18
申请号：US13025801
申请日：2011-02-11
申请人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Kent Evans , Julie Kelly , Ruth Berry
发明人： Thomas Buelter , Andrew Hawkins , Stephanie Porter-Scheinman , Peter Meinhold , Catherine Asleson Dundon , Aristos Aristidou , Jun Urano , Doug Lies , Matthew Peters , Melissa Dey , Justas Jancauskas , Kent Evans , Julie Kelly , Ruth Berry
IPC分类号： C12N1/00
CPC分类号： C12N15/81 , C12N9/0006 , C12P7/16 , C12Y101/01 , C12Y102/01005 , Y02E50/10 , Y02P20/52
摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含生物合成途径的重组微生物和使用所述重组微生物产生各种有益代谢物的方法。 在本发明的各个方面，重组微生物可以进一步包含一种或多种修饰，导致还原或消除3个酮酸（例如乙酰乳酸和2-乙酰-2-羟基丁酸酯）和/或醛衍生的副产物 。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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公开(公告)号：US20110076733A1
公开(公告)日：2011-03-31
申请号：US12855276
申请日：2010-08-12
申请人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Ruth Berry , Ishmeet Kalra
发明人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Ruth Berry , Ishmeet Kalra
CPC分类号： C12N15/81 , C12N9/0006 , C12N9/1022 , C12N9/88 , C12P7/16 , Y02E50/10
摘要： The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
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公开(公告)号：US20110020889A1
公开(公告)日：2011-01-27
申请号：US12820505
申请日：2010-06-22
申请人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
发明人： Reid M. Renny Feldman , Uvini Gunawardena , Jun Urano , Peter Meinhold , Aristos Aristidou , Catherine Asleson Dundon , Christopher Smith
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/88 , C12N9/90 , C12N15/81 , C12Y101/01001 , C12Y101/01008 , C12Y101/01086 , C12Y101/05003 , C12Y202/01006 , C12Y401/01001 , C12Y401/01074 , C12Y402/01009 , Y02E50/10
摘要： The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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8.发明申请METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS 审中-公开增加二羟酸脱水酶活性的方法，以提高燃料，化学品和氨基酸的生产公开(公告)号：US20120288910A1
公开(公告)日：2012-11-15
申请号：US13528106
申请日：2012-06-20
申请人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Lynne H. Albert
发明人： Jun Urano , Catherine Asleson Dundon , Peter Meinhold , Reid M. Renny Feldman , Aristos Aristidou , Andrew Hawkins , Thomas Buelter , Matthew Peters , Doug Lies , Stephanie Porter-Scheinman , Christopher Smith , Lynne H. Albert
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。 在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。 在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。 在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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9.发明申请METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS 有权增加二羟酸脱水酶活性的方法，以提高燃料，化学品和氨基酸的生产公开(公告)号：US20120028322A1
公开(公告)日：2012-02-02
申请号：US13246693
申请日：2011-09-27
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。 在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。 在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。 在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。 在本文所述的各种实施方案中，重组微生物可以是葡萄糖酵母的微生物，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，前WGD（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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10.发明授权Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids 有权增加二羟基酸脱水酶活性以改善燃料，化学品和氨基酸生产的方法公开(公告)号：US08071358B1
公开(公告)日：2011-12-06
申请号：US13228342
申请日：2011-09-08
CPC分类号： C12P7/16 , C07K14/395 , C12N9/88 , C12P13/02 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
摘要翻译： 本发明涉及包含一种或多种二羟基酸脱水酶（DHAD） - 生物合成途径的重组微生物，以及使用所述重组微生物产生源自所述DHAD需要生物合成途径的有益代谢物的方法。 在本发明的各个方面，重组微生物可被工程化以过表达一种或多种编码一种或多种Aft蛋白或其同系物的多核苷酸。 在一些实施方案中，重组微生物可以包含细胞溶质定位的DHAD酶。 在另外的实施方案中，重组微生物可以包含线粒体定位的DHAD酶。 在本文所述的各种实施方案中，重组微生物可以是葡萄糖酵母的微生物，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，前WGD（全基因组复制）酵母微生物 ，和非发酵酵母微生物。
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