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    Fermentative production of organic compounds
    4.
    发明授权
    Fermentative production of organic compounds 失效
    发酵生产有机化合物

    公开(公告)号：US08741599B2

    公开(公告)日：2014-06-03

    申请号：US12094626

    申请日：2006-11-27

    申请人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    发明人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    IPC分类号： C12P23/00 ,  C12P19/14 ,  C12P19/02 ,  C12P13/04 ,  C12P13/08 ,  C12P13/00 ,  C12P7/66 ,  C12P1/00 ,  C12P19/38 ,  C12P7/14 ,  C12N1/00 ,  C12N1/20

    CPC分类号： C12P13/08 ,  C12N1/20 ,  C12N1/22

    摘要： A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproducing the organic compound under fermentation conditions.

    摘要翻译： 一种用于发酵生产至少一种具有至少3个C原子或具有至少2个C原子和至少一个1 N原子的有机化合物的方法，包括以下步骤：a1）研磨淀粉原料，从而获得一种 包括淀粉原料的至少部分非淀粉样固体成分; a2）将研磨料悬浮在含水液体中，并通过酶液化和适当的后续糖化水解淀粉部分，由此获得包含单糖或寡糖的第一液体（1）; 和b）添加包含单糖或寡糖以及可代谢的单糖，二糖或寡糖的液体（1），或与包含至少50％浓度的可代谢的单糖，二糖或寡糖的组合物一起加入 并且其基本上不含不溶于包含微生物的发酵培养基的固体，所述微生物能够在发酵条件下过量产生有机化合物。

    Fermentative production of fine chemicals
    7.
    发明申请
    Fermentative production of fine chemicals 有权
    发酵精细化学品生产

    公开(公告)号：US20090162892A1

    公开(公告)日：2009-06-25

    申请号：US11597782

    申请日：2005-05-27

    申请人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    发明人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    IPC分类号： C12P23/00 ,  C12P1/00 ,  C12P21/00 ,  C12P19/38 ,  C12P19/30 ,  C12P19/12 ,  C12P19/04 ,  C12P13/04 ,  C12P17/18 ,  C12P17/12 ,  C12P7/64 ,  C12P7/40 ,  C12P7/48 ,  C12P7/46 ,  C12P7/42 ,  C12P7/26 ,  C12P7/02

    CPC分类号： C12P25/00 ,  C12P7/46 ,  C12P13/02 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/24 ,  C12P19/02 ,  C12P19/14 ,  C12P19/20 ,  Y02P20/582

    摘要： The invention relates to a process for the production of at least one microbial metabolite having at least 3 carbon atoms or at least 2 carbon atoms and at least 1 nitrogen atom by means of sugar-based microbial fermentation, comprising: a) the preparation of a sugar-containing liquid medium with a monosaccharide content of more than 20% by weight from a starch feedstock, the sugar-containing liquid medium also comprising non-starchy solid constituents of the starch feedstock; b) the fermentation of the sugar-containing liquid medium for the production of the metabolite(s); and c) depletion or isolation of at least one metabolite from the fermentation liquor, wherein a microorganism strain which produces the desired metabolite(s) is cultivated with the sugar-containing liquid medium, said liquid medium being obtained by: a1) milling the starch feedstock; and a2) liquefying the millbase in an aqueous liquid in the presence of at least one starch-liquefying enzyme, followed by saccharification using at least one saccharifying enzyme, where at least some of the millbase is liquefied by continuous or batchwise addition to the aqueous liquid.

    摘要翻译： 本发明涉及通过糖基微生物发酵生产具有至少3个碳原子或至少2个碳原子和至少1个氮原子的至少一种微生物代谢物的方法，包括：a）制备 含糖液体介质，其单糖含量大于淀粉原料的20重量％以上，所述含糖液体介质还包含淀粉原料的非淀粉状固体成分; b）用于生产代谢物的含糖液体培养基的发酵; 和c）从所述发酵液中消耗或分离至少一种代谢物，其中产生所需代谢物的微生物菌株与所述含糖液体培养基一起培养，所述液体培养基通过以下步骤获得：a1）研磨所述淀粉 原料; 和a2）在至少一种淀粉 - 液化酶的存在下在水性液体中液化所述研磨料，然后使用至少一种糖化酶进行糖化，其中至少一些所述絮凝液通过连续或分批添加至所述水性液体而液化 。

    Fermentative Production of Organic Compounds
    8.
    发明申请
    Fermentative Production of Organic Compounds 失效
    有机化合物的发酵生产

    公开(公告)号：US20080299606A1

    公开(公告)日：2008-12-04

    申请号：US12094626

    申请日：2006-11-27

    申请人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    发明人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    IPC分类号： C12P23/00 ,  C12P1/00 ,  C12P21/04 ,  C12P13/00 ,  C12P7/64 ,  C12P7/18 ,  C12P7/40 ,  C12P19/38 ,  C12P19/40 ,  C12P19/30

    CPC分类号： C12P13/08 ,  C12N1/20 ,  C12N1/22

    摘要： A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproducing the organic compound under fermentation conditions.

    摘要翻译： 一种用于发酵生产至少一种具有至少3个C原子或具有至少2个C原子和至少一个1 N原子的有机化合物的方法，包括以下步骤：a1）研磨淀粉原料，从而获得一种 包括淀粉原料的至少部分非淀粉样固体成分; a2）将研磨料悬浮在含水液体中，并通过酶液化和适当的后续糖化水解淀粉部分，由此获得包含单糖或寡糖的第一液体（1）; 和b）添加包含单糖或寡糖以及可代谢的单糖，二糖或寡糖的液体（1），或与包含至少50％浓度的可代谢的单糖，二糖或寡糖的组合物一起加入 并且其基本上不含不溶于包含微生物的发酵培养基的固体，所述微生物能够在发酵条件下过量产生有机化合物。

    Fermentative production of organic compounds
    9.
    发明授权
    Fermentative production of organic compounds 有权
    发酵生产有机化合物

    公开(公告)号：US08728762B2

    公开(公告)日：2014-05-20

    申请号：US12095130

    申请日：2006-11-27

    申请人： Matthias Boy ,  Stephan Freyer

    发明人： Matthias Boy ,  Stephan Freyer

    IPC分类号： C12P21/04 ,  C12P19/14 ,  C12P19/02 ,  C12P13/04 ,  C12P13/08 ,  C12P13/00 ,  C12P7/64 ,  C12P7/56 ,  C12P7/48 ,  C12P7/14 ,  C12N1/00 ,  C12N1/20

    CPC分类号： C12P19/14 ,  C12P13/08 ,  C12P13/14

    摘要： The invention relates to a method for producing at least one organic compound comprising at least 3 C-atoms or at least 2 C-atoms and at least 1 N-atom by fermentation. Said method comprises the following steps: i) a starch source is ground in order to obtain a grinding material which contains at least one part of the non-starch containing solid components of the starch source; ii) the grinding material is suspended in an aqueous liquid in an amount such that the dry mass content in the suspension is at least 45 wt. %, iii) the starch component is hydrolysed in the grinding material by liquefying and optionally, subsequently, sweetening, in order to obtain an aqueous medium M, which contains the hydrolysed starch components and at least one part of the non-starch containing solid components of the starch source, is obtained; and iv) the aqueous substance M obtained in step iii) is used for fermentation in order to cultivate a micro-organism which is capable of over-producing the organic compound; the suspension obtained in step ii) is heated in step iii) by introducing vapor into the suspension, in order to obtain temperatures greater than the pasting temperature of the starch obtained in the grinding materials.

    摘要翻译： 本发明涉及通过发酵生产至少一种含有至少3个C原子，至少2个C原子和至少1个N原子的有机化合物的方法。 所述方法包括以下步骤：i）研磨淀粉源以获得含有至少一部分淀粉源的非淀粉固体组分的研磨材料; ii）研磨材料悬浮在水性液体中，其量使得悬浮液中的干质量含量至少为45重量％。 ％，iii）淀粉组分在研磨材料中通过液化和任选地随后进行甜化而水解，以获得含有水解淀粉组分的水性介质M和至少一部分含淀粉的固体组分 的淀粉来源; 和iv）将步骤iii）中获得的水性物质M用于发酵，以培养能够过度生产有机化合物的微生物; 将步骤ii）中获得的悬浮液在步骤iii）中通过将蒸气引入悬浮液中来加热，以获得大于在研磨材料中获得的淀粉的糊化温度的温度。

    Method for the production of methionine
    10.
    发明授权
    Method for the production of methionine 有权
    生产甲硫氨酸的方法

    公开(公告)号：US07785846B2

    公开(公告)日：2010-08-31

    申请号：US10583304

    申请日：2004-12-17

    申请人： Matthias Boy ,  Daniela Klein ,  Hartwig Schröder

    发明人： Matthias Boy ,  Daniela Klein ,  Hartwig Schröder

    IPC分类号： C12P13/12

    CPC分类号： C07C319/28 ,  C12P13/12 ,  C07C323/58

    摘要： The present invention relates to a process for producing methionine by fermentation, a process for isolating the methionine formed, the methionine-containing biomass produced in isolation, use thereof for producing a feedstuff or feed supplement, and also to the use of the isolated methionine for producing foodstuffs or feedstuffs or food supplements or feed supplements.

    摘要翻译： 本发明涉及通过发酵生产甲硫氨酸的方法，分离形成的甲硫氨酸的方法，分离产生的含有甲硫氨酸的生物质，用于生产饲料或饲料添加剂的用途，以及将分离的甲硫氨酸用于 生产食品或饲料或食品补充剂或饲料添加剂。