公开(公告)号：US06810286B2

公开(公告)日：2004-10-26

申请号：US09799304

申请日：2001-03-05

申请人： Maura G. Donovan ,  Orhan Soykan ,  D. Curtis Deno ,  Lawrence J. Mulligan ,  Brian C. A. Fernandes

发明人： Maura G. Donovan ,  Orhan Soykan ,  D. Curtis Deno ,  Lawrence J. Mulligan ,  Brian C. A. Fernandes

IPC分类号： A61N100

CPC分类号： A61N1/326

摘要： Provided are novel stimulatory device for the controlled production of angiogenic growth factors. More specifically, a subthreshold pulse generator is used for the local production of vascular endothelial growth factor.

摘要翻译： 提供了用于受控制造血管生成生长因子的新型刺激装置。 更具体地，使用亚阈值脉冲发生器用于血管内皮生长因子的局部产生。

公开(公告)号：US08003373B2

公开(公告)日：2011-08-23

申请号：US12127908

申请日：2008-05-28

申请人： Orhan Soykan ,  Maura G. Donovan

发明人： Orhan Soykan ,  Maura G. Donovan

IPC分类号： C12M1/34

CPC分类号： A61B5/14546 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4839 ,  A61B5/7203 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/6428 ,  G01N21/8507 ,  G01N33/86 ,  G01N2021/6415 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2021/8528

摘要： Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy, at the second and third wavelengths.

摘要翻译： 基于酶的底物的切割量检测体液中酶的活化。 用两种荧光染料 - 供体和受体标记底物。 标记的基质被呈现给体液。 器件将第一波长的能量发射到体液中，并响应于第一波长的能量，检测由染料发射的第二和第三波长处的能量。 在衬底的酶切割之前，受体以响应于通过来自供体的荧光共振能量转移（FRET）接收的第二波长的能量而在第三波长处发射能量。 在酶切割底物之后，供体在第二波长处发射能量。 该装置可以基于在第二和第三波长处的能量的相对强度来确定体液中活化酶的浓度。

公开(公告)号：US07473548B2

公开(公告)日：2009-01-06

申请号：US10423112

申请日：2003-04-25

申请人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

发明人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

IPC分类号： C12M1/34

CPC分类号： A61B5/14546 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4839 ,  A61B5/7203 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/6428 ,  G01N21/8507 ,  G01N33/86 ,  G01N2021/6415 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2021/8528

摘要： Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy at the second and third wavelengths.

摘要翻译： 基于酶的底物的切割量检测体液中酶的活化。 用两种荧光染料 - 供体和受体标记底物。 标记的基质被呈现给体液。 器件将第一波长的能量发射到体液中，并响应于第一波长的能量，检测由染料发射的第二和第三波长处的能量。 在衬底的酶切割之前，受体以响应于通过来自供体的荧光共振能量转移（FRET）接收的第二波长的能量而在第三波长处发射能量。 在酶切割底物之后，供体在第二波长处发射能量。 该装置可以基于在第二和第三波长处的能量的相对强度来确定体液内活化酶的浓度。

公开(公告)号：US07155288B2

公开(公告)日：2006-12-26

申请号：US11300176

申请日：2005-12-14

申请人： Orhan Soykan ,  Maura G. Donovan

发明人： Orhan Soykan ,  Maura G. Donovan

IPC分类号： A61N1/08 ,  A61N63/00 ,  A61N1/18

CPC分类号： C12N5/0657 ,  A61N1/327 ,  A61N1/36042 ,  A61N1/362 ,  A61N1/37205 ,  A61N1/3756

摘要： An implantable system is provided that includes: a cell repopulation source comprising genetic material, undifferentiated and/or differentiated contractile cells, or a combination thereof capable of forming new contractile tissue in and/or near an infarct zone of a patient's myocardium; and an electrical stimulation device for electrically stimulating the new contractile tissue in and/or near the infarct zone of the patient's myocardium or otherwise damaged or diseased myocardial tissue.

公开(公告)号：US06824561B2

公开(公告)日：2004-11-30

申请号：US09745144

申请日：2000-12-20

申请人： Orhan Soykan ,  Maura G. Donovan

发明人： Orhan Soykan ,  Maura G. Donovan

IPC分类号： A61F206

CPC分类号： A61F2/88 ,  A61F2/02 ,  A61F2/06 ,  A61F2/82 ,  A61F2250/0001 ,  A61F2250/0067 ,  A61K9/0009 ,  A61K9/0024 ,  A61K38/4866 ,  A61K38/49 ,  A61K48/00 ,  A61K2035/126 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3843 ,  A61L27/54 ,  A61L29/005 ,  A61L29/16 ,  A61L31/005 ,  A61L31/10 ,  A61L31/16 ,  A61L2300/00 ,  A61L2300/22 ,  A61L2300/252 ,  A61L2300/254 ,  A61L2300/414 ,  A61L2300/416 ,  A61L2300/42 ,  A61L2300/602 ,  A61L2300/64 ,  A61N1/36542 ,  C12N5/069

摘要： An implantable system that includes a carrier and eukaryotic cells, which produce and release a therapeutic agent, and a stimulating element for stimulating the release of the therapeutic agent. The system can also include a sensing element for monitoring a physiological condition and triggering the stimulating element to stimulate the delivery device to release the therapeutic agent. Alternatively, the patient in which the system is implanted can activate the stimulating element to release the therapeutic agent.

摘要翻译： 包括产生和释放治疗剂的载体和真核细胞的可植入系统和用于刺激治疗剂释放的刺激元件。 该系统还可以包括用于监测生理状况并触发刺激元件以刺激输送装置释放治疗剂的感测元件。 或者，其中植入系统的患者可以激活刺激元件以释放治疗剂。

公开(公告)号：US20080227083A1

公开(公告)日：2008-09-18

申请号：US12127908

申请日：2008-05-28

申请人： Orhan Soykan ,  Maura G. Donovan

发明人： Orhan Soykan ,  Maura G. Donovan

IPC分类号： C12Q1/00 ,  A61B5/00

CPC分类号： A61B5/14546 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4839 ,  A61B5/7203 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/6428 ,  G01N21/8507 ,  G01N33/86 ,  G01N2021/6415 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2021/8528

摘要： Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy, at the second and third wavelengths.

摘要翻译： 基于酶的底物的切割量检测体液中酶的活化。 用两种荧光染料 - 供体和受体标记底物。 标记的基质被呈现给体液。 器件将第一波长的能量发射到体液中，并响应于第一波长的能量，检测由染料发射的第二和第三波长处的能量。 在衬底的酶切割之前，受体以响应于通过来自供体的荧光共振能量转移（FRET）接收的第二波长的能量而在第三波长处发射能量。 在酶切割底物之后，供体在第二波长处发射能量。 该装置可以基于在第二和第三波长处的能量的相对强度来确定体液中活化酶的浓度。

公开(公告)号：US06671558B1

公开(公告)日：2003-12-30

申请号：US09706531

申请日：2000-11-03

申请人： Orhan Soykan ,  Maura G. Donovan

发明人： Orhan Soykan ,  Maura G. Donovan

IPC分类号： A61N108

CPC分类号： A61N1/36042

摘要： An implantable system is provided that includes: a cell repopulation source comprising genetic material, undifferentiated and/or differentiated contractile cells, or a combination thereof capable of forming new contractile tissue in and/or near an infarct zone of a patient's myocardium; and an electrical stimulation device for electrically stimulating the new contractile tissue in and/or near the infarct zone of the patient's myocardium or otherwise damaged or diseased myocardial tissue.

摘要翻译： 提供了一种可植入系统，其包括：包含遗传材料，未分化和/或分化的收缩细胞或能够在患者心肌的梗塞区域内和/或附近形成新的收缩组织的组合的细胞增殖源; 以及电刺激装置，用于电刺激患者心肌梗死区或其它受损或患病心肌组织中和/或附近的新收缩组织。

公开(公告)号：US6151525A

公开(公告)日：2000-11-21

申请号：US145743

申请日：1998-09-02

申请人： Orhan Soykan ,  Maura G. Donovan

发明人： Orhan Soykan ,  Maura G. Donovan

IPC分类号： A61N1/36 ,  A61N1/372 ,  A61N1/08

CPC分类号： A61N1/36042 ,  A61N1/37205

摘要： An implantable system is provided that includes: a cell repopulation source comprising genetic material, undifferentiated contractile cells, or a combination thereof capable of forming new contractile tissue in and/or near an infarct zone of a patient's myocardium; and an electrical stimulation device for electrically stimulating the new contractile tissue in and/or near the infarct zone of the patient's myocardium.

摘要翻译： 提供了一种可植入系统，其包括：包含遗传物质，未分化的收缩细胞或能够在患者心肌的梗塞区域内和/或附近形成新的收缩组织的组合的细胞增殖源; 以及用于电刺激患者心肌梗死区域内和/或附近的新的收缩组织的电刺激装置。

公开(公告)号：US08940522B2

公开(公告)日：2015-01-27

申请号：US13214860

申请日：2011-08-22

申请人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

发明人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

IPC分类号： C12M3/00 ,  A61B5/145 ,  A61B5/00 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/64 ,  G01N21/85 ,  G01N33/86

CPC分类号： A61B5/14546 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4839 ,  A61B5/7203 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/6428 ,  G01N21/8507 ,  G01N33/86 ,  G01N2021/6415 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2021/8528

摘要： Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy at the second and third wavelengths.

摘要翻译： 基于酶的底物的切割量检测体液中酶的活化。 用两种荧光染料 - 供体和受体标记底物。 标记的基质被呈现给体液。 器件将第一波长的能量发射到体液中，并响应于第一波长的能量，检测由染料发射的第二和第三波长处的能量。 在衬底的酶切割之前，受体以响应于通过来自供体的荧光共振能量转移（FRET）接收的第二波长的能量而在第三波长处发射能量。 在酶切割底物之后，供体在第二波长处发射能量。 该装置可以基于在第二和第三波长处的能量的相对强度来确定体液内活化酶的浓度。

公开(公告)号：US20120046642A1

公开(公告)日：2012-02-23

申请号：US13214860

申请日：2011-08-22

申请人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

发明人： Orhan Soykan ,  Maura G. Donovan ,  Amy E. Thompson

IPC分类号： A61M5/168

CPC分类号： A61B5/14546 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4839 ,  A61B5/7203 ,  C12Q1/37 ,  C12Q1/56 ,  G01N21/6428 ,  G01N21/8507 ,  G01N33/86 ,  G01N2021/6415 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2021/8528

摘要： Activation of an enzyme in a bodily fluid is detected based on the amount of cleavage of a substrate for the enzyme. The substrate is tagged with two fluorescent dyes—a donor and an acceptor. The tagged substrate is presented to the bodily fluid. A device emits energy at a first wavelength into the bodily fluid, and detects energy at second and third wavelengths emitted by the dyes in response to the energy at the first wavelength. Prior to enzymatic cleavage of the substrate, the acceptor emits energy at the third wavelength in response to energy at the second wavelength received through fluorescent resonant energy transfer (FRET) from the donor. After enzymatic cleavage of the substrate, the donor emits energy at the second wavelength. The device can determine the concentration of activated enzyme within the bodily fluid based on the relative intensities of energy at the second and third wavelengths.

摘要翻译： 基于酶的底物的切割量检测体液中酶的活化。 用两种荧光染料 - 供体和受体标记底物。 标记的基质被呈现给体液。 器件将第一波长的能量发射到体液中，并响应于第一波长的能量，检测由染料发射的第二和第三波长处的能量。 在衬底的酶切割之前，受体以响应于通过来自供体的荧光共振能量转移（FRET）接收的第二波长的能量而在第三波长处发射能量。 在酶切割底物之后，供体在第二波长处发射能量。 该装置可以基于在第二和第三波长处的能量的相对强度来确定体液内活化酶的浓度。