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公开(公告)号：US08895298B2
公开(公告)日：2014-11-25
申请号：US10529453
申请日：2003-09-29
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
IPC分类号： A01N1/02 , C07K1/00 , C12N5/00 , C12N5/02 , C12N5/071 , G01N33/569 , G01N33/50 , B82Y30/00 , G01N33/574 , B01L3/00 , B01L9/00 , G01N33/543 , G01N1/40
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于从样品中分离细胞的方法（例如，将胎儿红细胞与母体血液分离）。 该方法开始于将包含细胞的样品引入一个或多个微流体通道。 在一个实施例中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞以产生进一步富集所需类型的细胞群体。 细胞的选择基于混合物中细胞的性质，例如，大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和膜通透性）或细胞内特性（例如，特定的表达 酶）。
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公开(公告)号：US20070231851A1
公开(公告)日：2007-10-04
申请号：US11726276
申请日：2007-03-21
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L1/52 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于从样品中分离细胞的方法（例如，将胎儿红细胞与母体血液分离）。 该方法开始于将包含细胞的样品引入一个或多个微流体通道。 在一个实施例中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞以产生进一步富集所需类型的细胞群体。 细胞的选择基于混合物中细胞的性质，例如，大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和膜通透性）或细胞内特性（例如，特定的表达 酶）。
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公开(公告)号：US08304230B2
公开(公告)日：2012-11-06
申请号：US11726230
申请日：2007-03-21
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于从样品中分离细胞的方法（例如，将胎儿红细胞与母体血液分离）。 该方法开始于将包含细胞的样品引入一个或多个微流体通道。 在一个实施例中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞以产生进一步富集所需类型的细胞群体。 细胞的选择基于混合物中细胞的性质，例如，大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和膜通透性）或细胞内特性（例如，特定的表达 酶）。
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公开(公告)号：US20060134599A1
公开(公告)日：2006-06-22
申请号：US10529453
申请日：2003-09-29
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于用于从样品中分离细胞的方法（例如，从母体分离胎儿红细胞开始于将包含细胞的样品引入一个或多个微流体通道。在一个实施方案中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞的选择基于混合物中细胞的性质，例如大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和 膜渗透性）或细胞内特性（例如特定酶的表达）。
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公开(公告)号：US08986966B2
公开(公告)日：2015-03-24
申请号：US11726231
申请日：2007-03-21
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
IPC分类号： C12M1/00 , C12N5/00 , C12N5/02 , C12N11/00 , C12N11/16 , B01L3/00 , B01L9/00 , B82Y30/00 , G01N33/50 , G01N33/543 , G01N33/569 , G01N33/574 , G01N1/40
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于从样品中分离细胞的方法（例如，将胎儿红细胞与母体血液分离）。 该方法开始于将包含细胞的样品引入一个或多个微流体通道。 在一个实施例中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞以产生进一步富集所需类型的细胞群体。 细胞的选择基于混合物中细胞的性质，例如，大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和膜通透性）或细胞内特性（例如，特定的表达 酶）。
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公开(公告)号：US20070259424A1
公开(公告)日：2007-11-08
申请号：US11726231
申请日：2007-03-21
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
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公开(公告)号：US08372579B2
公开(公告)日：2013-02-12
申请号：US11800940
申请日：2007-05-08
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
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公开(公告)号：US20120006760A1
公开(公告)日：2012-01-12
申请号：US13086370
申请日：2011-04-13
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L1/52 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
摘要翻译： 本发明的特征在于从样品中分离细胞的方法（例如，将胎儿红细胞与母体血液分离）。 该方法开始于将包含细胞的样品引入一个或多个微流体通道。 在一个实施例中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞以产生进一步富集所需类型的细胞群体。 细胞的选择基于混合物中细胞的性质，例如，大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和膜通透性）或细胞内特性（例如，特定的表达 酶）。
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公开(公告)号：US20070264675A1
公开(公告)日：2007-11-15
申请号：US11800940
申请日：2007-05-08
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
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公开(公告)号：US20070172903A1
公开(公告)日：2007-07-26
申请号：US11726230
申请日：2007-03-21
申请人： Mehmet Toner , George Truskey , Ravi Kapur
发明人： Mehmet Toner , George Truskey , Ravi Kapur
IPC分类号： G01N33/574 , C12M3/00
CPC分类号： B01L3/502753 , B01L3/502746 , B01L3/502761 , B01L9/54 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2200/12 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0883 , B01L2300/12 , B01L2400/0487 , B01L2400/086 , B33Y70/00 , B33Y80/00 , B82Y30/00 , C12N5/0087 , G01N1/40 , G01N1/405 , G01N33/5091 , G01N33/54366 , G01N33/54386 , G01N33/56966 , G01N33/574 , G01N2015/1006 , G01N2015/1081 , G01N2333/70582 , G01N2333/70589 , G01N2333/70596 , Y10T29/49982 , Y10T428/24744
摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
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