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公开(公告)号：US20090148858A1
公开(公告)日：2009-06-11
申请号：US11719528
申请日：2006-08-10
申请人： Mehul Patel , Dawn M. Schmidt , Sara H. Thrall , David G. Tew
发明人： Mehul Patel , Dawn M. Schmidt , Sara H. Thrall , David G. Tew
IPC分类号： G01N33/53
CPC分类号： C12Q1/25 , B01L3/50273 , B01L2300/16 , B01L2300/1827 , B01L2400/0478 , G01N33/53 , G01N33/54393 , G01N2500/02
摘要： Methods for characterizing a biochemical reaction and analysis of reaction products by establishing continuously variable concentration gradients of one or more reagents of the biochemical reaction are provided. Methods for determining mechanism of inhibition or activation, potency of inhibition or activation, or both of an enzyme inhibitor or activator, respectively, are also provided. The continuously variable concentration gradients can be established in a microfluidic chip.
摘要翻译： 提供了通过建立生化反应的一种或多种试剂的连续可变浓度梯度来表征生物化学反应和反应产物分析的方法。 还提供了用于分别确定抑制或活化机制，抑制或活化能力或酶抑制剂或活化剂两者的方法。 可以在微流控芯片中建立连续可变的浓度梯度。
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公开(公告)号：US07897330B2
公开(公告)日：2011-03-01
申请号：US11719528
申请日：2006-08-10
申请人： Mehul Patel , Dawn M. Schmidt , Sara H. Thrall , David G. Tew
发明人： Mehul Patel , Dawn M. Schmidt , Sara H. Thrall , David G. Tew
IPC分类号： C12Q1/00
CPC分类号： C12Q1/25 , B01L3/50273 , B01L2300/16 , B01L2300/1827 , B01L2400/0478 , G01N33/53 , G01N33/54393 , G01N2500/02
摘要： Methods for characterizing a biochemical reaction and analysis of reaction products by establishing continuously variable concentration gradients of one or more reagents of the biochemical reaction are provided. Methods for determining mechanism of inhibition or activation, potency of inhibition or activation, or both of an enzyme inhibitor or activator, respectively, are also provided. The continuously variable concentration gradients can be established in a microfluidic chip.
摘要翻译： 提供了通过建立生化反应的一种或多种试剂的连续可变浓度梯度来表征生物化学反应和反应产物分析的方法。 还提供了用于分别确定抑制或活化机制，抑制或活化能力或酶抑制剂或活化剂两者的方法。 可以在微流控芯片中建立连续可变的浓度梯度。
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公开(公告)号：US20090053814A1
公开(公告)日：2009-02-26
申请号：US11719513
申请日：2006-08-10
申请人： Mehul Patel , Daniel M. Hartmann , Joshua T. Nevill , Katya M. Prince , David G. Tew , Sara H. Thrall , Gregory A. Votaw , Hugh C. Crenshaw
发明人： Mehul Patel , Daniel M. Hartmann , Joshua T. Nevill , Katya M. Prince , David G. Tew , Sara H. Thrall , Gregory A. Votaw , Hugh C. Crenshaw
CPC分类号： G05D11/136 , B01F5/0646 , B01F5/0647 , B01F13/0059 , B01F15/0404 , B01F15/042 , B01L3/50273 , B01L3/502738 , B01L3/565 , B01L7/52 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , F04B19/006 , G01N2035/00158 , G05D7/0694 , Y10T436/10
摘要： The pumps (Pn) are operated to transport individual reagent streams into the chip in a non-pulsatile, laminar flow regime at low flow rates permitting lows grading from 0 to as little as 5 nl/min with a precision of 0.1 nl/min. In the chip (MFC), the reagent streams are merged and the reagents mixed to form a reaction product. The reaction product can be measured at one or more detection points defined in the chip. Concentration gradients are continuously varied by continuously varying the flow rates respectively produced by the pumps according to predetermined flow velocity profiles.
摘要翻译： 操作泵（Pn）以低流速以非脉动，层流状态将各个试剂流输送到芯片中，允许以0.1nl / min的精度从0降至低至5nl / min。 在芯片（MFC）中，将试剂流合并，将试剂混合形成反应产物。 可以在芯片中定义的一个或多个检测点处测量反应产物。 通过根据预定的流速分布连续改变由泵产生的流量，浓度梯度连续变化。
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公开(公告)号：US20090145485A1
公开(公告)日：2009-06-11
申请号：US11719521
申请日：2006-08-10
申请人： Gregory Fenton Smith , Dawn M. Schmidt , Sara Thrall , David G. Tew , Gregory A. Votaw , Hugh C. Crenshaw , Michael G. Pollack , Christopher David Bevan , Kelly Junge , Mehul Patel
发明人： Gregory Fenton Smith , Dawn M. Schmidt , Sara Thrall , David G. Tew , Gregory A. Votaw , Hugh C. Crenshaw , Michael G. Pollack , Christopher David Bevan , Kelly Junge , Mehul Patel
CPC分类号： B01F15/0404 , B01F5/0646 , B01F5/0647 , B01F5/0653 , B01F13/0059 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/565 , B01L2200/027 , B01L2300/0816 , B01L2300/0867 , B01L2300/1894 , B01L2400/0487 , B01L2400/0677 , F16K99/0001 , F16K99/0032 , F16K99/0044 , F16K2099/0084 , G01N30/6095 , G01N35/1097 , Y10T137/0324 , Y10T137/0329 , Y10T137/0391 , Y10T137/218 , Y10T137/2196 , Y10T137/86131 , Y10T137/87652
摘要： According to one embodiment, an apparatus and method for delivering one or more fluids to a microfluidic channel is provided. A microfluidic channel is provided in communication with a first conduit for delivering fluids to the microfluidic channel. Further, the apparatus and method can include a first fluid freeze valve connected to the first conduit and operable to reduce the temperature of the first conduit for freezing fluid in the first conduit such that fluid is prevented from advancing through the first conduit.
摘要翻译： 根据一个实施例，提供了将一种或多种流体输送到微流体通道的装置和方法。 提供与第一导管连通以将流体输送到微流体通道的微流体通道。 此外，该装置和方法可以包括连接到第一管道的第一流体冷冻阀，并可操作以降低第一管道的温度，以冷冻第一管道中的流体，以防止流体通过第一管道前进。
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公开(公告)号：US07048926B2
公开(公告)日：2006-05-23
申请号：US10407028
申请日：2003-04-04
申请人： Martin Brandt , Walter E. DeWolf, Jr. , Paul M. Keller , Arunbhai H. Patel , David J. Payne , Shannon L. Reed , Mark A. Seefeld , David G. Tew , Nicola G. Wallis , Joshua M. West
发明人： Martin Brandt , Walter E. DeWolf, Jr. , Paul M. Keller , Arunbhai H. Patel , David J. Payne , Shannon L. Reed , Mark A. Seefeld , David G. Tew , Nicola G. Wallis , Joshua M. West
CPC分类号： A61K31/4375 , Y02A50/48
摘要： The invention provides methods for using agonists and antagonists of FabK polypeptides, particularly to modulate the metabolism of bacteria or to treat bacterial infection.
摘要翻译： 本发明提供使用FabK多肽的激动剂和拮抗剂，特别是调节细菌代谢或治疗细菌感染的方法。
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