公开(公告)号：US20240075473A1

公开(公告)日：2024-03-07

申请号：US17929350

申请日：2022-09-02

申请人： Mesa Biotech, Inc.

发明人： Mark Nowakowski ,  Michael Wang ,  Robert B Cary ,  Hong Cai ,  Conrad Lindberg ,  Martin Bouliane ,  Donald J Thomas

IPC分类号： B01L3/00

CPC分类号： B01L3/502746 ,  B01L3/502761 ,  B01L3/502769 ,  B01L2300/048 ,  B01L2300/0822 ,  B01L2400/0457

摘要： A lateral flow assay device of the present disclosure has numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. The vent pockets have protrusions to help prevent accidental resealing. The device also can have a gasket to ensure free air movement between open vent pockets. Flow control features that protrude horizontally into a chamber proximal to an outlet act to deflect the flow to the side of the chamber opposite from the outlet, prior to the flow entering the outlet. Such features can reduce the flow velocity of the sample fluid and increase the effective fluid flow path length, enhance mixing of reagents with a sample, and enabling more accurate control of fluid flow in the device. A flexible circuit with patterned metallic electrical components disposed on a heat stable material can be in direct contact with fluid in the chambers and has resistive heating elements aligned with the vent pockets and the chambers.

公开(公告)号：US12023672B2

公开(公告)日：2024-07-02

申请号：US17929350

申请日：2022-09-02

申请人： Mesa Biotech, Inc.

发明人： Mark Nowakowski ,  Michael Wang ,  Robert B Cary ,  Hong Cai ,  Conrad Lindberg ,  Martin Bouliane ,  Donald J Thomas

IPC分类号： B01L3/00

CPC分类号： B01L3/502746 ,  B01L3/502761 ,  B01L3/502769 ,  B01L2300/048 ,  B01L2300/0822 ,  B01L2400/0457

摘要： A lateral flow assay device of the present disclosure has numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. The vent pockets have protrusions to help prevent accidental resealing. The device also can have a gasket to ensure free air movement between open vent pockets. Flow control features that protrude horizontally into a chamber proximal to an outlet act to deflect the flow to the side of the chamber opposite from the outlet, prior to the flow entering the outlet. Such features can reduce the flow velocity of the sample fluid and increase the effective fluid flow path length, enhance mixing of reagents with a sample, and enabling more accurate control of fluid flow in the device. A flexible circuit with patterned metallic electrical components disposed on a heat stable material can be in direct contact with fluid in the chambers and has resistive heating elements aligned with the vent pockets and the chambers.

公开(公告)号：US12128414B2

公开(公告)日：2024-10-29

申请号：US17929369

申请日：2022-09-02

申请人： Mesa Biotech, Inc.

发明人： Mark Nowakowski ,  Michael Wang ,  Robert B Cary ,  Hong Cai ,  Conrad Lindberg ,  Martin Bouliane ,  Donald J Thomas

IPC分类号： B01L7/00 ,  B01L3/00 ,  C12Q1/04 ,  C12Q1/6816 ,  C12Q1/6844

CPC分类号： B01L7/52 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502769 ,  C12Q1/04 ,  C12Q1/6816 ,  C12Q1/6844 ,  B01L3/502715 ,  B01L2200/0621 ,  B01L2200/0689 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/048 ,  B01L2300/0822 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0445 ,  B01L2400/0457 ,  B01L2400/0638 ,  B01L2400/0677 ,  B01L2400/0694 ,  C12Q2531/113 ,  C12Q2560/00 ,  C12Q1/6816 ,  C12Q2565/629

摘要： An automated method for performing an assay of the present disclosure can be performed in a microfluidic device that is a lateral flow device having numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. Each chamber can have a reagent recess proximal to an inlet end of the chamber. A reagent pellet formed in a reagent recess can be effectively mixed with a sample as the sample flows into the chamber. A flexible circuit with patterned metallic electrical components disposed on a heat stable material can be in direct contact with fluid in the chambers and has resistive heating elements aligned with, for example, a chamber for performing an amplification reaction. A lateral flow detection chamber can include a capillary pool proximal to a sample receiving end of a lateral flow strip, providing effective mixing and dispersion of a sample with detection particles, as well as enhancing, uniformity of particle migration on the detection strip. The microfluidic device can be configured to be hermetically sealed, thereby preventing contamination of a testing environment.

公开(公告)号：US20240076724A1

公开(公告)日：2024-03-07

申请号：US17929369

申请日：2022-09-02

申请人： Mesa Biotech, Inc.

发明人： Mark Nowakowski ,  Michael Wang ,  Robert B Cary ,  Hong Cai ,  Conrad Lindberg ,  Martin Bouliane ,  Donald J Thomas

IPC分类号： C12Q1/6844 ,  B01L3/00 ,  C12Q1/04

CPC分类号： C12Q1/6844 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502761 ,  C12Q1/04 ,  B01L2400/0445 ,  B01L2400/0638 ,  C12Q2531/113 ,  C12Q2560/00

摘要： An automated method for performing an assay of the present disclosure can be performed in a microfluidic device that is a lateral flow device having numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. Each chamber can have a reagent recess proximal to an inlet end of the chamber. A reagent pellet formed in a reagent recess can be effectively mixed with a sample as the sample flows into the chamber. A flexible circuit with patterned metallic electrical components disposed on a heat stable material can be in direct contact with fluid in the chambers and has resistive heating elements aligned with, for example, a chamber for performing an amplification reaction. A lateral flow detection chamber can include a capillary pool proximal to a sample receiving end of a lateral flow strip, providing effective mixing and dispersion of a sample with detection particles, as well as enhancing, uniformity of particle migration on the detection strip. The microfluidic device can be configured to be hermetically sealed, thereby preventing contamination of a testing environment.

公开(公告)号：US20160310948A1

公开(公告)日：2016-10-27

申请号：US15137983

申请日：2016-04-25

申请人： Mesa Biotech, Inc.

发明人： Mark Nowakowski ,  Michael Wang ,  Robert B. Cary ,  Hong Cai ,  Conrad Lindberg ,  Martin Bouliane ,  Donald J. Thomas

IPC分类号： B01L7/00 ,  C12Q1/68 ,  B01L3/00

CPC分类号： B01L7/52 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/0689 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0677 ,  B01L2400/0694 ,  C12Q1/6816 ,  C12Q2565/629

摘要： A disposable cassette for detecting nucleic acids or performing other assays. The cassette can be inserted into a base station during use. The cassette has numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. The vent pockets have protrusions to help prevent accidental resealing. The cassette also can have a gasket to ensure free air movement between open vent pockets. A flexible circuit with patterned metallic electrical components disposed on a heat stable material can be in direct contact with fluid in the chambers and has resistive heating elements aligned with the vent pockets and the chambers. The detection chamber, which houses a lateral flow detection strip can have a space below the strip that has sufficient capacity to accommodate an entire volume of the sample fluid entering the detection chamber at a height that enables the fluid to flow up the detection strip by capillary action without flooding or otherwise bypassing regions of the detection strip. The space can also contain detection particles. Recesses in in the cassette channels or chambers can have structures such as ridges or grooves to direct fluid flow to enhance rehydration of lyophilized reagents disposed in the recess. Flow diverters in the chambers can reduce the flow velocity of the sample fluid and increase the effective fluid flow path length, enabling more accurate control of fluid flow in the cassette.

摘要翻译： 用于检测核酸或执行其他测定的一次性盒。 在使用过程中，盒可插入基站。 该盒具有许多特征，以确保装置在重力作用下的正确操作，例如当排气口未密封时能使样品流体从一个室流到下一个室的通气口。 排气口具有突起，有助于防止意外重新密封。 盒式磁带还可以具有垫圈，以确保敞开的排气口之间的自由空气运动。 具有设置在热稳定材料上的图案化金属电气部件的柔性电路可以与腔室中的流体直接接触并且具有与排气口和腔室对准的电阻加热元件。 容纳侧向流动检测条的检测室可以具有在条带下方的空间，其具有足够的容量以容纳进入检测室的整个体积的样品流体，使得流体能够通过毛细管向上流过检测条 动作不会溢出或以其他方式绕过检测条的区域。 该空间还可以包含检测颗粒。 盒式通道或腔室中的凹陷可以具有诸如脊或凹槽的结构，以引导流体流动以增强设置在凹部中的冻干试剂的再水化。 腔室中的分流器可以降低样品流体的流速并增加有效的流体流动路径长度，使得能够更准确地控制盒中的流体流动。