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    MONITORING OF FRYING OIL QUALITY USING COMBINED OPTICAL INTERROGATION METHODS AND DEVICES
    6.
    发明申请
    MONITORING OF FRYING OIL QUALITY USING COMBINED OPTICAL INTERROGATION METHODS AND DEVICES 有权
    使用组合光学方法和装置监测油脂质量

    公开(公告)号：US20110059532A1

    公开(公告)日：2011-03-10

    申请号：US12920514

    申请日：2009-03-02

    申请人： Ai-Ping Wei ,  Milind Balwant Sabade ,  Abolghasssen B. Mahmoodi

    发明人： Ai-Ping Wei ,  Milind Balwant Sabade ,  Abolghasssen B. Mahmoodi

    IPC分类号： G01N33/26 ,  G01N33/03 ,  G01N30/00

    CPC分类号： G01N33/03 ,  G01N21/643 ,  G01N21/78 ,  G01N31/22

    摘要： Herein are disclosed methods and devices for optically monitoring multiple parameters of an oil sample. In one embodiment, the methods and devices can be used for determining the quality of cooking or flying oil in terms of the free fatty acid content and total polar compound content of the oil. The methods use an optical absorbtive/reflective property in evaluating the free fatty acid content, and use optical fluorescence in evaluating the total polar compound content, with both measurements using a single sampling substrate and a single measuring device.

    摘要翻译： 本文公开了用于光学监测油样品的多个参数的方法和装置。 在一个实施方案中，方法和装置可以用于根据游离脂肪酸含量和油的总极性化合物含量来确定烹饪或飞行油的质量。 该方法在评估游离脂肪酸含量时使用光学吸收/反射性质，并且使用光学荧光来评估总极性化合物含量，两次测量使用单个取样基板和单个测量装置。

    Assay procedure using fluorogenic tracers
    8.
    发明授权
    Assay procedure using fluorogenic tracers 失效

    公开(公告)号：US07153654B2

    公开(公告)日：2006-12-26

    申请号：US10286600

    申请日：2002-10-31

    申请人： Ai-Ping Wei ,  James N. Herron

    发明人： Ai-Ping Wei ,  James N. Herron

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

    CPC分类号： G01N33/582 ,  C12Q1/6818

    摘要： Fluorescent energy transfer dyes capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope or nucleic acid for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.

    Fluorogenic protease substrates based on dye-dimerization
    10.
    发明申请
    Fluorogenic protease substrates based on dye-dimerization 失效
    基于染料二聚化的荧光蛋白酶底物

    公开(公告)号：US20050089946A1

    公开(公告)日：2005-04-28

    申请号：US10930522

    申请日：2004-08-31

    申请人： Ai-Ping Wei ,  Michael Williams

    发明人： Ai-Ping Wei ,  Michael Williams

    IPC分类号： C07K7/06 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/68

    CPC分类号： C07K7/06 ,  C12Q1/37 ,  C12Q2337/40 ,  Y02A50/451

    摘要： A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a flexible peptide, the dye groups being of proximity sufficiently close so as to allow free energy attractions to draw the dyes together to essentially self-quench fluorescence of the dye groups, wherein self quenching of fluorescence of the dye groups is effected by dye dimerization or stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye dimerization or stacking, thereby producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.

    摘要翻译： 一种生物测定方法包括以下步骤：提供包含与柔性肽结合的两个荧光染料基团的酶底物，所述染料组的接近度足够接近，以便使自由能吸引力将染料一起吸收到基本上自熄灭荧光 的染料基团，其中染料基团的荧光自发淬灭通过染料二聚化或堆叠进行，并且酶切割肽以使荧光染料基团从染料二聚化或堆叠释放，从而产生荧光强度的增加。 还公开了用于本发明方法的蛋白酶底物。 本发明用于微生物的检测和鉴定，灭菌保证，药物发现，酶测定，免疫测定和其他生物测定。