摘要:
Compositions comprising granulocyte-colony stimulating factor (GCSF) produced in a strain of Pichia pastoris glycoengineered to produce a GCSF wherein greater than 18% of the molecules comprise an 0-glycan with one mannose per (0-glycan is described. In particular aspects, the GCSF is PEGylated at the JV-terminus.
摘要:
Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.
摘要:
Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.
摘要:
Methods for producing proteins and glycoproteins in Pichia pastoris that lack detectable cross binding activity to antibodies made against host cell antigens are described. In particular, methods are described wherein recombinant Pichia pastoris strains that do not display a β-mannosyltransferase 2 activity with respect to an N-glycan or O-glycan and do not display at least one activity selected from a β-mannosyltransferase 1, 3, and 4 activity to produce recombinant proteins and glycoproteins. These recombinant Pichia pastoris strains can produce proteins and glycoproteins that lack detectable α-mannosidase resistant β-mannose residues thereon and thus, lack cross binding activity to antibodies against host cell antigens. Further described are methods for producing bi-sialylated human erythropoietin in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens.
摘要:
The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additionally, the present invention relates to pharmaceutical compositions comprising an antibody having a particular enriched N-glycan structure, wherein said N-glycan structure is GlcNAcMan5GlcNAc2.
摘要:
The disclosure discloses a method for querying group's dispatching area information, which comprises: a user sending a request for querying group's dispatching area information to a base station through a user terminal; and the base station querying the group's dispatching area information according to request for querying the group's dispatching area information, and returning a response to the group's dispatching area information query to the user terminal. The disclosure also discloses a query system for the querying group's dispatching area information correspondingly. The present invention can improve the validity, the dispatching reliability and accuracy of the group call.
摘要:
In a method and system for sensing current in a switching regulator (SWR) operating in a current mode, a power switch is coupled to receive the current from a switching element, the power switch being controlled by a gate signal. An inrush of the current causes an initial transient spike (ITS). A buffer having a buffer input and a buffer output is coupled to receive the gate signal and provide a buffered gate signal. The buffer output is disabled during the ITS. A sense switch (SW) is coupled to receive a portion of the current from the switching element, the SW being turned on by the buffered gate signal after the initial transient spike. A sense resistor (SR) is coupled to receive the portion of the current from the SW. An amplifier converts the portion of the current through the SR to a voltage signal for controlling the SWR.
摘要:
Compositions and methods for producing compositions comprising immunoglobulins or immunoglobulin fragments having an N-linked glycosylation pattern consisting predominantly of the GlCNAcMan3GlcNAc2 N-glycan structure are disclosed. The GlCNAcMan3GlcNAc2 N-glycan structure effects an increase in binding to the FcγRiπ receptors and a decrease in binding to the FcγRH receptors.
摘要:
The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additionally, the present invention relates to pharmaceutical compositions comprising an antibody having a particular enriched N-glycan structure, wherein said N-glycan structure is GalGlcNAcMan5GlcNAc2.
摘要:
In a method and system for sensing current in a switching regulator (SWR) operating in a current mode, a power switch is coupled to receive the current from a switching element, the power switch being controlled by a gate signal. An inrush of the current causes an initial transient spike (ITS). A buffer having a buffer input and a buffer output is coupled to receive the gate signal and provide a buffered gate signal. The buffer output is disabled during the ITS. A sense switch (SW) is coupled to receive a portion of the current from the switching element, the SW being turned on by the buffered gate signal after the initial transient spike. A sense resistor (SR) is coupled to receive the portion of the current from the SW. An amplifier converts the portion of the current through the SR to a voltage signal for controlling the SWR.