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1.发明申请System and method for providing dynamic network firewall with default deny 审中-公开提供动态网络防火墙与默认拒绝的系统和方法公开(公告)号：US20070255861A1
公开(公告)日：2007-11-01
申请号：US11498624
申请日：2006-08-03
IPC分类号： G06F3/00
CPC分类号： H04L63/029 , H04L63/0218 , H04L63/0263
摘要： A computing system having host computer and an I/O processor (IOP) provides firewall services to the host computer. When the host computer and the IOP are initialized, all of the communication ports are reset to a closed state. Application programs are loaded into memory of the host computer for execution and provide the identity of communication ports to be used by the application. The identity of the requested communication ports are used to instruct the IOP to open the communication port to accept network data packets that use the particular port. When the application terminates operation, the communication ports used by the application are closed to provide dynamic control over communication ports. This process ensures that only ports currently used by applications currently executing within the host computer are open without administrator action.
摘要翻译： 具有主计算机和I / O处理器（IOP）的计算系统向主计算机提供防火墙服务。 当主计算机和IOP被初始化时，所有通信端口都被复位到关闭状态。 将应用程序加载到主计算机的存储器中以供执行，并提供要由应用程序使用的通信端口的标识。 所请求的通信端口的身份用于指示IOP打开通信端口以接受使用特定端口的网络数据包。 当应用程序终止操作时，应用程序使用的通信端口关闭，以提供对通信端口的动态控制。 此过程确保当前正在主机中执行的应用程序当前使用的端口在没有管理员操作的情况下打开。
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2.发明授权Reduced-footprint illumination system using highly-efficient optical collection and reflection surface(s) and method for manufacturing optical elements used therein 失效使用高效光学收集和反射表面的减少照明照明系统和用于制造其中使用的光学元件的方法公开(公告)号：US07764435B2
公开(公告)日：2010-07-27
申请号：US11675252
申请日：2007-02-15
IPC分类号： G02B27/30
CPC分类号： G02B27/0927 , G02B5/02 , G02B27/0994
摘要： An improved optical collection and transmission surface includes a plastic mold injection surface including staggered multiple transmission sites each with a recessed portion in which incident light is not lost during reflection towards the propagation surface. A device implementing the surfaces is plastic-mold injected of high quality acrylic at in a preferred embodiment and can be economically optionally polished or finished to achieve desired quality surfaces.
摘要翻译： 改进的光学收集和透射表面包括塑料模具注射表面，该塑料模具注射表面包括交错的多个传输部位，每个传输部位具有凹入部分，入射光在向着传播表面反射期间不会丢失。 实施这些表面的装置是在优选实施例中由高质量丙烯酸注塑的塑料模具，并且可经济地任选地抛光或精加工以实现所需的质量表面。
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公开(公告)号：US08334272B2
公开(公告)日：2012-12-18
申请号：US12902240
申请日：2010-10-12
CPC分类号： A61K31/7088 , A61K48/0008 , C12N15/1137 , C12N15/64 , C12N2310/14
摘要： The present invention provides methods for increasing the transfer of nucleic acids into cells. In particular, the present invention provides for the use of inhibitors of HDAC6, a cytoplasmic histone deacetylase present in mammalian cells by, for example, small molecules or siRNA treatment, in increasing gene transfer and/or expression in cells in vitro and in vivo for research and gene therapy applications.
摘要翻译： 本发明提供了增加核酸向细胞转移的方法。 特别地，本发明提供了通过例如小分子或siRNA处理在哺乳动物细胞中存在的HDAC6的抑制剂，其在体外和体内增加细胞中的基因转移和/或表达的用途， 研究和基因治疗应用。
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公开(公告)号：US20110021608A1
公开(公告)日：2011-01-27
申请号：US12902240
申请日：2010-10-12
IPC分类号： A61K48/00 , C12N15/87 , A61K31/713 , A61P43/00
CPC分类号： A61K31/7088 , A61K48/0008 , C12N15/1137 , C12N15/64 , C12N2310/14
摘要： The present invention provides methods for increasing the transfer of nucleic acids into cells. In particular, the present invention provides for the use of inhibitors of HDAC6, a cytoplasmic histone deacetylase present in mammalian cells by, for example, small molecules or siRNA treatment, in increasing gene transfer and/or expression in cells in vitro and in vivo for research and gene therapy applications.
摘要翻译： 本发明提供了增加核酸向细胞转移的方法。 特别地，本发明提供了通过例如小分子或siRNA处理在哺乳动物细胞中存在的HDAC6的抑制剂，其在体外和体内增加细胞中的基因转移和/或表达的用途， 研究和基因治疗应用。
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5.发明申请METHOD AND COMPOSITION FOR ALVEOLAR EPITHELIAL CELL-SPECIFIC NUCLEIC ACID NUCLEAR IMPORT 审中-公开上皮细胞特异性核酸核进口的方法和组合公开(公告)号：US20130197064A1
公开(公告)日：2013-08-01
申请号：US13634422
申请日：2011-03-14
申请人： David A. Dean
发明人： David A. Dean
IPC分类号： C12N15/113 , C12N15/85 , A61K48/00
CPC分类号： C12N15/113 , A61K48/00 , A61K48/0075 , C07K14/47 , C12N15/85 , C12N2800/107
摘要： One aspect of the invention relates to an isolated nuclear targeting molecule that includes a fragment of a mammalian glycoprotein 36 (gp36, also known as T1-α or podoplanin) gene expressed in type I alveolar epithelial cells. Plasmids containing the isolated nuclear targeting molecule which are useful for affording nuclear uptake of the plasmid DNA in type I alveolar epithelial cells but not type II alveolar epithelial cells, and compositions and host cells containing such plasmids are also disclosed. Use of the plasmids for targeting an exogenous DNA into nuclei of type I alveolar epithelial cells is described herein.
摘要翻译： 本发明的一个方面涉及分离的核靶向分子，其包括在I型肺泡上皮细胞中表达的哺乳动物糖蛋白36（gp36，也称为T1-α或podoplanin）基因的片段。 还公开了含有分离的核靶向分子的质粒，其可用于提供I型肺泡上皮细胞中但不是II型肺泡上皮细胞的质粒DNA的核摄取，以及含有这种质粒的组合物和宿主细胞。 本文描述了将外源DNA靶向到I型肺泡上皮细胞核的用途。
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6.发明授权Fuel injection sytem employing solid-state injectors for liquid fueled combustion engines 失效燃油喷射系统采用液体燃料内燃机的固态喷射器
公开(公告)号：US5437255A
公开(公告)日：1995-08-01
申请号：US212989
申请日：1994-03-15
申请人： Mark L. Sadley , David A. Dean
发明人： Mark L. Sadley , David A. Dean
IPC分类号： F02M31/125 , F02M51/06 , F02M53/02 , F02M61/00 , F02M61/02 , F02M69/04 , F02M37/04 , F02B3/00
CPC分类号： F02M69/041 , F02M31/125 , F02M51/06 , F02M51/0603 , F02M53/02 , F02M61/00 , F02M61/02 , F02M69/04 , F02B2275/14 , Y02T10/123 , Y02T10/126
摘要： A surface array of electrically actuated pulsed microscopic fuel jets arranged on the interior surface of a mixing chamber injects fuel into air to form a combustible air-fuel mixture. The jets are solid state units with no moving parts, which can be actuated individually or in groups, and the volume of fuel injected per each jet's firing pulse can be varied by changing the characteristics of the electrical actuating pulse. Having no moving parts and being electrically activated, the jets are intrinsically suited to direct computer control. By providing a large number of such jets and a means of their direct electronic control via negative feedback, fine control of the operating parameters of the combustion engine and a high degree of redundancy to compensate for the failure of individual jets is made possible. The lack of moving parts and the absence of a high pressure fuel supply system provide significant advantages in performance, cost, reliability, and safety over the prior art in fuel injection.
摘要翻译： 布置在混合室的内表面上的电致动的脉冲显微燃料喷嘴的表面阵列将燃料喷射到空气中以形成可燃空气 - 燃料混合物。 射流是没有移动部件的固态单元，其可以单独地或分组地启动，并且每个喷射器的点火脉冲喷射的燃料的体积可以通过改变电致动脉冲的特性来改变。 没有移动部件并被电激活，喷射器本质上适合于直接计算机控制。 通过提供大量这样的射流和通过负反馈进行直接电子控制的手段，可以精确控制内燃机的操作参数和高度的冗余度来补偿单个喷气机的故障。 缺少运动部件和没有高压燃料供应系统在燃料喷射方面在现有技术中的性能，成本，可靠性和安全性方面提供了显着的优点。
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公开(公告)号：US20090069266A1
公开(公告)日：2009-03-12
申请号：US12163152
申请日：2008-06-27
IPC分类号： A61K31/7088 , C12N15/09
CPC分类号： A61K31/7088 , A61K48/0008 , C12N15/1137 , C12N15/64 , C12N2310/14
摘要： The present invention provides compositions and methods for increasing the transfer of nucleic acids into cells. In particular, the present invention provides for the use of inhibitors of HDAC6, a cytoplasmic histone deacetylase present in mammalian cells by, for example, small molecules or siRNA treatment, in increasing gene transfer and/or expression in cells in vitro and in vivo for research and gene therapy applications.
摘要翻译： 本发明提供了增加核酸向细胞转移的组合物和方法。 特别地，本发明提供了通过例如小分子或siRNA处理在哺乳动物细胞中存在的HDAC6的抑制剂，其在体外和体内增加细胞中的基因转移和/或表达的用途， 研究和基因治疗应用。
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公开(公告)号：US5827705A
公开(公告)日：1998-10-27
申请号：US822982
申请日：1997-03-21
申请人： David A. Dean
发明人： David A. Dean
CPC分类号： C12N15/85 , C12N15/79 , C12N15/90 , C12N2800/108 , C12N2830/42
摘要： The invention provides a nuclear targeting molecule having a nucleic acid sequence which consists of SEQ ID NO:1 or a nuclear targeting portion of SEQ ID NO:1. The invention further provides a plasmid for targeting a DNA molecule into the nucleus of a host cell. The plasmid comprises the nuclear targeting molecule (having SEQ ID NO:1 or a nuclear targeting portion of SEQ ID NO:1) and a DNA molecule to be targeted to a nucleus. This plasmid of the subject invention can be introduced into various host cells, and the nuclear targeting molecule will target the DNA molecule to the nucleus of the host cell. Thus, the invention further provides a method of targeting a DNA molecule into the nucleus of a host cell. The method comprises providing a plasmid (the plasmid comprising the nuclear targeting molecule and the DNA molecule to be targeted) and introducing the plasmid into the cytoplasm of the host cell. In this method, the nuclear targeting molecule targets the DNA molecule into the nucleus of the host cell.
摘要翻译： 本发明提供了具有由SEQ ID NO：1或SEQ ID NO：1的核靶向部分组成的核酸序列的核靶向分子。 本发明还提供了将DNA分子靶向宿主细胞核的质粒。 质粒包含核靶向分子（具有SEQ ID NO：1或SEQ ID NO：1的核靶向部分）和靶向细胞核的DNA分子。 本发明的该质粒可以被引入到各种宿主细胞中，核靶向分子将DNA分子靶向宿主细胞的核。 因此，本发明还提供了将DNA分子靶向宿主细胞核的方法。 该方法包括提供质粒（包含核靶向分子的质粒和待靶向的DNA分子），并将质粒导入宿主细胞的细胞质中。 在该方法中，核靶向分子将DNA分子靶向宿主细胞的核。
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9.发明授权Cell-specific molecule and method for importing DNA into osteoblast nuclei 有权细胞特异性分子和将DNA导入成骨细胞核的方法公开(公告)号：US07741113B2
公开(公告)日：2010-06-22
申请号：US11410579
申请日：2006-04-24
CPC分类号： A61K48/0058 , C07K14/4702 , C07K2319/09 , C12N15/85 , C12N2800/30 , C12N2830/001 , C12N2830/008 , C12N2830/15 , C12N2830/42 , C12N2830/85 , C12N2840/20
摘要： A plasmid, viral or linear DNA molecule containing a nucleic acid sequence derived from the promoter region of the hCol1α2 gene, which is selectively transported into the nuclei of cells in the osteoblast lineage. The sequence can be used independently as a nuclear entry sequence only, and/or as a nuclear entry sequence without regard to position, in a vector or linear DNA that directs gene expression and nuclear entry. The disclosure further includes a chimeric DNA sequence derived by the addition of osteoblast-specific enhancer sequences to the nuclear entry sequence/promoter sequence, to increase osteoblast-specific expression while retaining osteoblast-specific nuclear import. An enhancer sequence is derived from the promoter region of the human Core Binding Factor alpha 1 (Cbfa1/Runx2) gene. The Cbfa1/Runx2 promoter can be added to the sequence derived from, or alternatively, comprising the promoter region of the hCol1α2 gene. Also provided are methods of use of the novel sequences.
摘要翻译： 含有衍生自hCol1α2基因的启动子区域的核酸序列的质粒，病毒或线性DNA分子，其被选择性地转运到成骨细胞谱系中的细胞核中。 该序列可以独立地用作核进入序列，和/或作为指导基因表达和核进入的载体或线性DNA中不考虑位置的核进入序列。 本公开还包括通过向核进入序列/启动子序列添加成骨细胞特异性增强子序列而衍生的嵌合DNA序列，以增加成骨细胞特异性表达，同时保留成骨细胞特异性核进口。 增强子序列衍生自人核心结合因子α1（Cbfa1 / Runx2）基因的启动子区。 可以将Cbfa1 / Runx2启动子加入到由hCol1α2基因的启动子区域衍生或替代地包含hCol1α2基因的启动子区域的序列中。 还提供了使用新序列的方法。
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公开(公告)号：US07604798B2
公开(公告)日：2009-10-20
申请号：US11182933
申请日：2005-07-15
申请人： David A. Dean
发明人： David A. Dean
CPC分类号： C12N15/87 , A61K48/00 , C12N2830/008
摘要： The present invention relates to methods and compositions for importing DNA into the nuclei of a specific cell type. In particular, the present invention provides methods and compositions for specifically targeting the nuclei of pulmonary cells.
摘要翻译： 本发明涉及将DNA导入特定细胞型细胞核的方法和组合物。 特别地，本发明提供了用于特异性靶向肺细胞核的方法和组合物。
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