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公开(公告)号:US20060030038A1
公开(公告)日:2006-02-09
申请号:US11234656
申请日:2005-09-22
申请人: Michael Taylor , Phillip Belgrader , Farzad Pourahmadi , William McMillan , Ronald Chang , Jesus Ching
发明人: Michael Taylor , Phillip Belgrader , Farzad Pourahmadi , William McMillan , Ronald Chang , Jesus Ching
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/087 , B01L2300/14 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12M47/06
摘要: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
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公开(公告)号:US20080057572A1
公开(公告)日:2008-03-06
申请号:US11977697
申请日:2007-10-24
申请人: Kurt Petersen , Michael Taylor , Farzad Pourahmadi , William McMillan , Ronald Chang , Stanley Sakai , Jesus Ching , Douglas Dority , Phillip Belgrader , M. Northrup
发明人: Kurt Petersen , Michael Taylor , Farzad Pourahmadi , William McMillan , Ronald Chang , Stanley Sakai , Jesus Ching , Douglas Dority , Phillip Belgrader , M. Northrup
IPC分类号: C12M3/00
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译: 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体,裂解细胞或病毒以从其中分离分析物(例如核酸),以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断,生命科学研究,环境或法医应用,用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号:US20060027686A1
公开(公告)日:2006-02-09
申请号:US11233629
申请日:2005-09-22
申请人: Michael Taylor , Phillip Belgrader , Farzad Pourahmadi , William McMillan , Ronald Chang , Stanley Sakai , Jesus Ching
发明人: Michael Taylor , Phillip Belgrader , Farzad Pourahmadi , William McMillan , Ronald Chang , Stanley Sakai , Jesus Ching
CPC分类号: B01L3/502 , B01L2200/10 , B01L2300/0681 , B01L2400/0487 , B01L2400/0644 , B01L2400/0655 , C12M47/06
摘要: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
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公开(公告)号:US09073053B2
公开(公告)日:2015-07-07
申请号:US11233629
申请日:2005-09-22
申请人: Michael T. Taylor , Phillip Belgrader , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching
发明人: Michael T. Taylor , Phillip Belgrader , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching
CPC分类号: B01L3/502 , B01L2200/10 , B01L2300/0681 , B01L2400/0487 , B01L2400/0644 , B01L2400/0655 , C12M47/06
摘要: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
摘要翻译: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器,以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子,用于破裂细胞或病毒。
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公开(公告)号:US08268603B2
公开(公告)日:2012-09-18
申请号:US11234656
申请日:2005-09-22
申请人: Michael T. Taylor , Phillip Belgrader , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Jesus Ching
发明人: Michael T. Taylor , Phillip Belgrader , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Jesus Ching
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/087 , B01L2300/14 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12M47/06
摘要: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
摘要翻译: 用于破坏细胞或病毒的装置包括具有用于保持细胞或病毒的室的容器。 容器包括限定腔室的至少一个柔性壁。 该装置还包括用于冲击柔性壁的外表面的换能器,以在腔室中产生压力波。 该装置还包括用于增加腔室中的压力的压力源。 室的加压确保换能器与柔性壁之间的有效耦合。 该装置还可以包括室中的珠子,用于破裂细胞或病毒。
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公开(公告)号:US08580559B2
公开(公告)日:2013-11-12
申请号:US11977615
申请日:2007-10-24
申请人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
IPC分类号: C12M1/34
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译: 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体,裂解细胞或病毒以从其中分离分析物(例如核酸),以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断,生命科学研究,环境或法医应用,用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号:US06881541B2
公开(公告)日:2005-04-19
申请号:US09800590
申请日:2001-03-06
申请人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
IPC分类号: C12M1/33 , B01L3/00 , B01L7/00 , C12M1/26 , C12N1/00 , G01N1/04 , G01N1/10 , G01N33/48 , C12Q1/68
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: A method for extracting nucleic acid from a fluid sample comprises the steps of introducing the sample into a cartridge having a sample flow path and a lysing chamber in the sample flow path. The lysing chamber contains at least one filter for separating cells or viruses from the sample. The sample is forced to flow through the lysing chamber to capture the cells or viruses with the filter, while used sample fluid flows to waste. The captured cells or viruses are disrupted to release their nucleic acid, the nucleic acid is eluted from the lysing chamber, and optionally the nucleic acid is amplified and detected in a reaction chamber of the cartridge.
摘要翻译: 从流体样品中提取核酸的方法包括以下步骤:将样品引入具有样品流动路径中的样品流动路径和裂解室的盒中。 裂解室含有至少一个用于从样品中分离细胞或病毒的过滤器。 样品被迫流过裂解室以用过滤器捕获细胞或病毒,而使用的样品流体流入废物。 捕获的细胞或病毒被破坏以释放其核酸,核酸从裂解室洗脱,并且任选地在核心的反应室中扩增和检测核酸。
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公开(公告)号:US20120171758A1
公开(公告)日:2012-07-05
申请号:US11977615
申请日:2007-10-24
申请人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译: 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体,裂解细胞或病毒以从其中分离分析物(例如核酸),以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断,生命科学研究,环境或法医应用,用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号:US20120009667A1
公开(公告)日:2012-01-12
申请号:US13081060
申请日:2011-04-06
申请人: KURT E. PETERSON , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人: KURT E. PETERSON , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译: 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体,裂解细胞或病毒以从其中分离分析物(例如核酸),以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断,生命科学研究,环境或法医应用,用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号:US09789481B2
公开(公告)日:2017-10-17
申请号:US11977697
申请日:2007-10-24
申请人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人: Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
CPC分类号: B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
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