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    β1,3-galactosyltransferase and DNA encoding the same
    2.
    发明授权
    β1,3-galactosyltransferase and DNA encoding the same 有权
    β1,3-半乳糖基转移酶和编码相同的DNA

    公开(公告)号：US07189550B2

    公开(公告)日：2007-03-13

    申请号：US11227177

    申请日：2005-09-16

    申请人： Tetsuo Endo ,  Satoshi Koizumi

    发明人： Tetsuo Endo ,  Satoshi Koizumi

    IPC分类号： C12N15/54 ,  C12N9/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21

    CPC分类号： C12N9/1051

    摘要： The present invention provides a protein having β1,3-galactosyltransferase activity, a DNA encoding the protein, a transformant comprising the DNA, a process for producing the protein using the transformant, and a process for producing a galactose-containing complex carbohydrate using the transformant.

    摘要翻译： 本发明提供了具有β1,3-半乳糖基转移酶活性的蛋白质，编码该蛋白质的DNA，包含该DNA的转化体，使用该转化体产生该蛋白质的方法，以及使用该转化体产生含半乳糖的复合碳水化合物的方法 。

    Process for producing GDP-fucose
    4.
    发明授权
    Process for producing GDP-fucose 失效
    生产GDP-岩藻糖的方法

    公开(公告)号：US06875591B1

    公开(公告)日：2005-04-05

    申请号：US09631709

    申请日：2000-08-03

    申请人： Satoshi Koizumi ,  Hiroshi Nagano ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

    发明人： Satoshi Koizumi ,  Hiroshi Nagano ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

    IPC分类号： C12P19/32 ,  C12P19/30 ,  C12P19/02

    CPC分类号： C12P19/32

    摘要： Processes for producing GDP-fucose, comprising allowing GKDM and a culture broth of a microorganism capable of converting GKDM into GDP-fucose to be present in an aqueous medium, forming and accumulating GDP-fucose therein, and recovering the GDP-fucose therefrom; or comprising allowing a GTP precursor, a saccharide, a culture broth of a microorganism capable of forming GTP from a GTP precursor, and a culture broth of a microorganism capable of forming GKDM from a saccharide and GTP to be present in an aqueous medium, forming and accumulating GKDM therein, converting the accumulated GKDM into GDP-fucose using as a culture broth of a microorganism capable of converting GKDM into GDP-fucose to form and accumulate GDP-fucose therein, and recovering the GDP-fucose therefrom; and a process for producing GKDM, comprising allowing a GTP precursor, a saccharide, a culture broth of a microorganism capable of forming GTP from a GTP precursor, and a culture broth of a microorganism capable forming GKDM from a saccharide and GTP to be present in an aqueous medium, forming and accumulating GKDM therein, and recovering the GKDM therefrom.

    摘要翻译： 生产GDP-岩藻糖的方法，包括使GKDM和能够将GKDM转化为GDP-岩藻糖的微生物的培养液存在于水性介质中，形成并积累其中的GDP-岩藻糖，并从其中回收GDP-岩藻糖; 或者包括使GTP前体，糖类，能够从GTP前体形成GTP的微生物的培养液和能够从糖和GTP形成GKDM的微生物的培养液存在于水性介质中，形成 并在其中积累GKDM，使用能够将GKDM转化为GDP-岩藻糖的微生物的培养液将积累的GKDM转化为GDP-岩藻糖，以形成和积累GDP-岩藻糖，并从其中回收GDP-岩藻糖; 以及生产GKDM的方法，包括使来自GTP前体的能够形成GTP的微生物的GTP前体，糖类，培养液以及能够从糖和GTP形成GKDM的微生物的培养液存在于 水介质，在其中形成和积聚GKDM，并从中回收GKDM。

    Processes for producing sugar nucleotides and complex carbohydrates
    5.
    发明申请
    Processes for producing sugar nucleotides and complex carbohydrates 有权

    公开(公告)号：US20050019874A1

    公开(公告)日：2005-01-27

    申请号：US10940026

    申请日：2004-09-14

    申请人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

    发明人： Satoshi Koizumi ,  Katsutoshi Sasaki ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

    IPC分类号： C12P19/30 ,  C12N9/10

    CPC分类号： C12P19/30

    摘要： This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.

    Glycosyltransferase and DNA encoding the same
    6.
    发明授权
    Glycosyltransferase and DNA encoding the same 有权
    糖基转移酶和编码相同的DNA

    公开(公告)号：US06830908B2

    公开(公告)日：2004-12-14

    申请号：US10218381

    申请日：2002-08-15

    申请人： Satoshi Koizumi ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

    发明人： Satoshi Koizumi ,  Tetsuo Endo ,  Kazuhiko Tabata ,  Akio Ozaki

    IPC分类号： C12N910

    CPC分类号： C12N9/1051 ,  C12N9/1048 ,  C12P19/18 ,  Y10S435/822

    摘要： The present invention provides a protein having &bgr;1,4-galactosyltransferase activity, DNA encoding the protein, a recombinant DNA comprising the DNA, a transformant carrying the recombinant DNA, a process for producing &bgr;1,4-galactosyltransferase by using the transformant, and a process for producing a galactose-containing carbohydrate by using the transformant.

    摘要翻译： 本发明提供了具有β1,4-半乳糖基转移酶活性的蛋白质，编码该蛋白质的DNA，包含DNA的重组DNA，携带重组DNA的转化体，使用该转化体产生β1,4-半乳糖基转移酶的方法， 用于通过使用转化体产生含半乳糖的碳水化合物。

    Mannose isomerase and DNA encoding the enzyme
    7.
    发明授权
    Mannose isomerase and DNA encoding the enzyme 失效
    甘露糖异构酶和编码酶的DNA

    公开(公告)号：US06673581B1

    公开(公告)日：2004-01-06

    申请号：US09958548

    申请日：2001-10-11

    申请人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

    发明人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

    IPC分类号： C12P1924

    CPC分类号： C12P19/24 ,  C12N9/90 ,  C12Y503/01007

    摘要： The present invention provides a novel protein having mannose isomerase activity, DNA encoding the protein, a recombinant vector comprising the DNA, a transformant obtained by introducing the recombinant vector into a host cell, and processes for producing the above protein and mannose, fructose, xylulose and lyxose by using the transformant.

    摘要翻译： 本发明提供了具有甘露糖异构酶活性的新型蛋白质，编码该蛋白质的DNA，包含DNA的重组载体，将重组载体导入宿主细胞获得的转化体，以及产生上述蛋白质和甘露糖，果糖，木酮糖的方法 并通过使用转化体来研究。

    Process for producing N-acetylneuraminic acid
    8.
    发明授权
    Process for producing N-acetylneuraminic acid 有权
    N-乙酰神经氨酸生产方法

    公开(公告)号：US07579175B2

    公开(公告)日：2009-08-25

    申请号：US10338872

    申请日：2003-01-09

    申请人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

    发明人： Satoshi Koizumi ,  Kazuhiko Tabata ,  Tetsuo Endo ,  Akio Ozaki

    IPC分类号： C12P7/58 ,  C12P1/00 ,  C12Q1/00 ,  C12N9/24 ,  C12N1/20 ,  C02F3/34 ,  C07K14/00 ,  C07H21/04

    CPC分类号： C12P19/26

    摘要： The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.

    摘要翻译： 本发明提供了经济地生产N-乙酰神经氨酸的方法，而不使用昂贵的材料如丙酮酸和磷酸烯醇丙酮酸。 该方法包括：（i）具有N-乙酰神经氨酸醛缩酶活性或N-乙酰神经氨酸合成酶活性的微生物的培养物或培养物的处理物质，（ii）能够产生丙酮酸的微生物培养物或 培养物的处理物质，或能够产生磷酸烯醇丙酮酸或培养物处理物质的微生物的培养物，（iii）N-乙酰甘露糖胺，和（iv）形成丙酮酸所必需的能量源或 磷酸烯醇式丙酮酸存在于水性介质中以在水性介质中形成并积聚N-乙酰神经氨酸; 并从水性介质中回收N-乙酰神经氨酸。

    Process for producing alpha1,4-galactosyltransferase and galactose-containing complex carbohydrate
    9.
    发明授权
    Process for producing alpha1,4-galactosyltransferase and galactose-containing complex carbohydrate 失效
    制备α1,4-半乳糖基转移酶和含半乳糖的复合碳水化合物的方法

    公开(公告)号：US07517673B2

    公开(公告)日：2009-04-14

    申请号：US11734869

    申请日：2007-04-13

    申请人： Tetsuo Endo ,  Satoshi Koizumi

    发明人： Tetsuo Endo ,  Satoshi Koizumi

    IPC分类号： C12P19/00 ,  C12P19/02 ,  C12N9/10 ,  C12N1/21 ,  C07H21/04

    CPC分类号： C12P19/18 ,  C12N9/1051 ,  C12P19/26

    摘要： The present invention can provide a process for producing a protein having α1.4-galactosyltransferase activity using a transformant comprising a DNA encoding a protein having α1.4-galactosyltransferase activity derived from a microorganism belonging to the genus Pasteurella and a process for producing a galactose-containing complex carbohydrate using a transformant capable of producing a protein having α1.4-galactosyltransferase activity derived from a microorganism.

    摘要翻译： 本发明可以提供使用包含编码来源于巴斯德氏菌属的微生物的具有α1.4-半乳糖基转移酶活性的蛋白质的DNA的转化体和产生半乳糖的方法来生产具有α1.4-半乳糖基转移酶活性的蛋白质的方法 使用能够产生衍生自微生物的具有α1.4-半乳糖基转移酶活性的蛋白质的转化体的复合碳水化合物。

    Process for producing α1,4-galactosyltransferase and galactose-containing complex sugar
    10.
    发明授权
    Process for producing α1,4-galactosyltransferase and galactose-containing complex sugar 失效
    制备α1,4-半乳糖基转移酶和含半乳糖的复合糖的方法

    公开(公告)号：US07232674B2

    公开(公告)日：2007-06-19

    申请号：US10490879

    申请日：2002-09-26

    申请人： Tetsuo Endo ,  Satoshi Koizumi

    发明人： Tetsuo Endo ,  Satoshi Koizumi

    IPC分类号： C12N9/10 ,  C12N1/19 ,  C12Q1/68 ,  C12P21/06 ,  C12P13/04 ,  C07H21/04

    CPC分类号： C12P19/18 ,  C12N9/1051 ,  C12P19/26

    摘要： The present invention can provide a process for producing a protein having α1.4-galactosyltransferase activity using a transformant comprising a DNA encoding a protein having α1.4-galactosyltransferase activity derived from a microorganism belonging to the genus Pasteurella and a process for producing a galactose-containing complex carbohydrate using a transformant capable of producing a protein having α1,4-galactosyltransferase activity derived from a microorganism.

    摘要翻译： 本发明可以提供使用包含编码来源于巴斯德氏菌属的微生物的具有α1.4-半乳糖基转移酶活性的蛋白质的DNA的转化体和产生半乳糖的方法来生产具有α1.4-半乳糖基转移酶活性的蛋白质的方法 使用能够产生衍生自微生物的具有α1,4-半乳糖基转移酶活性的蛋白质的转化体的复合碳水化合物。