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    Methods and DNA Constructs for Autoregulating Transgene Silencing
    1.
    发明申请
    Methods and DNA Constructs for Autoregulating Transgene Silencing 有权

    公开(公告)号：US20210207155A1

    公开(公告)日：2021-07-08

    申请号：US17206113

    申请日：2021-03-18

    Applicant: Monsanto Technology LLC

    Inventor： Edwards Allen ,  Larry A. Gilbertson ,  Sergey I. Ivashuta ,  James K. Roberts

    IPC: C12N15/82

    Abstract: This invention provides a method to autoregulate expression of a transgene susceptible to sRNA silencing by concomitantly transcribing RNA from DNA of a transgene and RNA from DNA from at least one sRNA silencing pathway gene. An aspect of the invention provides use of a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator. Also disclosed are transgenic cells and organisms having in their genome a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator.

    Methods and DNA Constructs for Autoregulating Transgene Silencing
    3.
    发明申请
    Methods and DNA Constructs for Autoregulating Transgene Silencing 审中-公开
    用于自动调节转基因沉默的方法和DNA构建体

    公开(公告)号：US20160068852A1

    公开(公告)日：2016-03-10

    申请号：US14945091

    申请日：2015-11-18

    Applicant: Monsanto Technology LLC

    Inventor： Edwards Allen ,  Larry A. Gilbertson ,  Sergey I. Ivashuta ,  James K. Roberts

    IPC: C12N15/82

    CPC classification number: C12N15/8218 ,  C12N15/8216 ,  C12N15/8274 ,  C12N15/8285 ,  C12N15/8286

    Abstract: This invention provides a method to autoregulate expression of a transgene susceptible to sRNA silencing by concomitantly transcribing RNA from DNA of a transgene and RNA from DNA from at least one sRNA silencing pathway gene. An aspect of the invention provides use of a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator. Also disclosed are transgenic cells and organisms having in their genome a recombinant DNA construct that includes DNA of a transgene and DNA of an sRNA silencing regulator.

    Abstract translation: 本发明提供了一种通过从来自至少一个sRNA沉默途径基因的DNA的转基因和RNA的DNA同时转录RNA来自动调节对sRNA沉默敏感的转基因的表达的方法。 本发明的一个方面提供了包含sRNA沉默调节子的转基因DNA和DNA的重组DNA构建体的用途。 还公开了在其基因组中具有重组DNA构建体的转基因细胞和生物体，其包含转基因的DNA和sRNA沉默调节子的DNA。

    Recombinant DNA Constructs and Methods for Modulating Expression of a Target Gene
    4.
    发明申请
    Recombinant DNA Constructs and Methods for Modulating Expression of a Target Gene 审中-公开
    重组DNA构建体和调控靶基因表达的方法

    公开(公告)号：US20150247154A1

    公开(公告)日：2015-09-03

    申请号：US14691281

    申请日：2015-04-20

    Applicant: Monsanto Technology LLC

    Inventor： Sergey I. Ivashuta ,  Barbara E. Wiggins ,  Yuanji Zhang

    IPC: C12N15/82

    CPC classification number: C12N15/8218 ,  C12N15/111 ,  C12N15/8216 ,  C12N15/8261 ,  C12N2310/141 ,  Y02A40/146

    Abstract: This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

    Abstract translation: 本发明通过干扰小RNA与其靶基因的结合来提供重组DNA构建体和操纵由小RNA调节的靶基因表达的方法。 更具体地，本发明公开了编码切割阻断剂的重组DNA构建体，5-修饰的切割阻断剂和用于调节靶基因表达的翻译抑制剂及其使用方法。 进一步公开的是可用于设计重组DNA构建体的miRNA靶标，包括miRNA-无反应转基因，miRNA诱饵，切割阻断剂，5-修饰的切割阻断剂和翻译抑制剂，以及其使用方法，以及含有这种构建体的转基因真核细胞和生物 。

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