-
公开(公告)号：US09708620B2
公开(公告)日：2017-07-18
申请号：US14582303
申请日：2014-12-24
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Larry A. Gilbertson , Sara E. Heisel , Shihshieh Huang , Elysia K. Krieger , Thomas M. Malvar
IPC: C12N15/82 , C12N15/113
CPC classification number: C12N15/8218 , C12N15/113 , C12N15/8216 , C12N15/8217 , C12N15/8286 , C12N2310/113 , Y02A40/162
Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
-
公开(公告)号：US09315819B2
公开(公告)日：2016-04-19
申请号：US14191825
申请日：2014-02-27
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Sara E. Heisel , Sergey Ivashuta , Elysia K. Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
CPC classification number: C12N15/8216 , C12N15/8217 , C12N15/8218 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
Abstract translation: 本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。 更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。 还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。
-
公开(公告)号：US10793869B2
公开(公告)日：2020-10-06
申请号：US15637859
申请日：2017-06-29
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Edwards M. Allen , Larry A Gilbertson , Sara E. Heisel , Shihshieh Huang , Elysia K. Krieger , Thomas M. Malvar
IPC: C12N15/82 , C12N15/113
Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
-
公开(公告)号：US11008580B2
公开(公告)日：2021-05-18
申请号：US15958026
申请日：2018-04-20
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Edwards M. Allen , Sara E. Heisel , Sergey Ivashuta , Elysia K. Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
IPC: C12N15/82
Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
-
公开(公告)号：US10301623B2
公开(公告)日：2019-05-28
申请号：US15052062
申请日：2016-02-24
Applicant: Monsanto Technology LLC
Inventor： Edwards Allen , Liang Guo , Sara E. Heisel , Sergey I. Ivashuta , Yuanji Zhang
IPC: C12N15/82 , C12N15/113
Abstract: This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.
-
Patent Agency Ranking
公开(公告)号：US20150166993A1
公开(公告)日：2015-06-18
申请号：US14567786
申请日：2014-12-11
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalic , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
IPC: C12N15/113 , C12N15/82
CPC classification number: C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
Abstract translation: 本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。 包括显示营养反应表达的新型miRNA和miRNA前体。 还公开了miRNA诱饵序列。 还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。
-
公开(公告)号：US20140317780A1
公开(公告)日：2014-10-23
申请号：US14191825
申请日：2014-02-27
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Sara E. Heisel , Sergey Ivashuta , Elysia K. Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
IPC: C12N15/82
CPC classification number: C12N15/8216 , C12N15/8217 , C12N15/8218 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
Abstract translation: 本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。 更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。 还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。
-
公开(公告)号：US10435686B2
公开(公告)日：2019-10-08
申请号：US14567786
申请日：2014-12-11
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalic , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
IPC: C12N15/113 , C12N15/82
Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
-
公开(公告)号：US09976152B2
公开(公告)日：2018-05-22
申请号：US15065416
申请日：2016-03-09
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Sara E. Heisel , Sergey Ivashuta , Elysia K. Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
CPC classification number: C12N15/8218 , C12N15/8216 , C12N15/8217 , C12N15/8225 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
-
公开(公告)号：US20160186194A1
公开(公告)日：2016-06-30
申请号：US15065416
申请日：2016-03-09
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Sara E. Heisel , Sergey Ivashuta , Elysia K. Krieger , Jennifer Lutke , Robert J. Meister , Yuanji Zhang
IPC: C12N15/82
CPC classification number: C12N15/8218 , C12N15/8216 , C12N15/8217 , C12N15/8225 , C12N15/8234 , C12N15/8238 , C12N15/8254 , C12N15/8265 , C12N15/827 , C12N15/8275 , C12N15/8279 , C12N15/8285 , C12N15/8286 , C12N15/8289 , Y02A40/162 , Y02A40/164
Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
Abstract translation: 本发明提供用于在植物或植物害虫或病原体中时间特异性控制基因表达的分子构建体和方法。 更具体地，本发明提供具有新颖的昼夜节律表达模式的植物miRNA基因，其可用于设计用于至少一种基因的时间特异性表达的重组DNA构建体。 还提供了在其基因组中含有本发明的重组DNA构建体的非天然转基因植物细胞，植物和种子。
-
-
-
-
-
-
-
-
-
Search Results
13
records
(took 0.024 seconds)
