公开(公告)号：US5198335A

公开(公告)日：1993-03-30

申请号：US608014

申请日：1990-10-31

申请人： Nobuyoshi Sekikawa ,  Harumi Katsuyama ,  Asaji Kondo

发明人： Nobuyoshi Sekikawa ,  Harumi Katsuyama ,  Asaji Kondo

IPC分类号： C12Q1/32 ,  C12Q1/58 ,  G01N31/22 ,  G01N33/84

CPC分类号： C12Q1/58 ,  C12Q1/32 ,  G01N31/223 ,  G01N33/84 ,  Y10T436/177692 ,  Y10T436/25125

摘要： An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising:(I) a light-transmissive, liquid-impermeable support:(II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia;(III) a liquid barrier layer which allows gaseous ammonia passing therethrough;(IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia;(V) an intrinsic ammonia trapping layer containing a reagent which acts on intrinsic ammonia to convert it into a form which can not reach said reaction layer; and(IV) a porous spreading layer, in a laminate form.

摘要翻译： 一种用于分析氨形成基材的整体多层分析元件，包括：（I）透光液体不可渗透的载体：（II）含有能够通过气态氨进行可检测变化的试剂的氨指示剂层; （III）允许气态氨通过其中的液体阻挡层; （IV）氨形成底物反应层，其含有可与氨形成基质反应形成气态氨的试剂; （V）含有作用于本征氨的试剂以将其转化为不能到达所述反应层的形式的试剂的本征氨捕集层; 和（IV）层叠形式的多孔铺展层。

公开(公告)号：US4548906A

公开(公告)日：1985-10-22

申请号：US609291

申请日：1984-05-11

申请人： Nobuyoshi Sekikawa ,  Harumi Katsuyama ,  Asaji Kondo

发明人： Nobuyoshi Sekikawa ,  Harumi Katsuyama ,  Asaji Kondo

IPC分类号： C12Q1/34 ,  C12Q1/58 ,  G01N31/22 ,  G01N33/52 ,  G01N33/62 ,  G01N33/70

CPC分类号： C12Q1/34 ,  C12Q1/58 ,  G01N33/526 ,  G01N33/62 ,  G01N2333/978 ,  Y10S435/805 ,  Y10S435/807 ,  Y10T436/175383

摘要： An integral multilayer analytical element for the analysis of ammonia or an ammonia forming substrate in a liquid sample is disclosed. The element comprises a transparent support on which an indicator layer for ammonia, a liquid blocking layer, a reagent layer incorporated with a reagent which may react with the substrate to form ammonia, and a spreading layer, are integrally superposed in this order. The improvement is that the liquid blocking layer is made of a porous material which is permeable to ammonia but substantially impermeable to liquids.

摘要翻译： 公开了一种用于液体样品中氨或氨形成基质分析的整体多层分析元件。 该元件包括透明支撑体，其上依次叠置有用于氨的指示剂层，液体阻挡层，与可与基底反应形成氨的试剂的试剂层和扩散层。 改进之处在于，液体阻挡层由可渗透氨但基本上不透液体的多孔材料制成。

公开(公告)号：US4503145A

公开(公告)日：1985-03-05

申请号：US352970

申请日：1982-02-26

申请人： Harumi Katsuyama ,  Yoshikazu Amano ,  Asaji Kondo

发明人： Harumi Katsuyama ,  Yoshikazu Amano ,  Asaji Kondo

IPC分类号： G01N33/66 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/48 ,  C12Q1/52 ,  G01N31/22 ,  G01N33/92 ,  G01N33/52

CPC分类号： C12Q1/52 ,  C12Q1/26 ,  Y10S435/805

摘要： An analysis film for assaying transaminase activity comprises a reagent layer containing pyruvic acid oxidase, a combination of a hydrogen donor and a coupler--which is capable of forming a cationic dye--and an anionic polymer. The cationic dye formed by the reaction with a color indicator composition for detecting hydrogen peroxide is fixed with the anionic polymer, thereby mobilization and diffusion of the cationic dye being prevented so that the analysis film achieves high detection sensitivity and high accuracy.

摘要翻译： 用于测定转氨酶活性的分析膜包括含有丙酮酸氧化酶，氢供体和能够形成阳离子染料的成色剂的组合的试剂层和阴离子聚合物。 通过与用于检测过氧化氢的颜色指示剂组合物的反应形成的阳离子染料用阴离子聚合物固定，从而阻止阳离子染料的移动和扩散，使得分析膜实现高检测灵敏度和高精度。

公开(公告)号：US4478942A

公开(公告)日：1984-10-23

申请号：US352969

申请日：1982-02-26

申请人： Harumi Katsuyama ,  Yoshikazu Amano ,  Asaji Kondo

发明人： Harumi Katsuyama ,  Yoshikazu Amano ,  Asaji Kondo

IPC分类号： G01N33/50 ,  C12Q1/28 ,  G01N31/00 ,  G01N31/22 ,  G01N33/52 ,  G01N33/70 ,  G01N33/92

CPC分类号： G01N33/523 ,  C12Q1/28 ,  C12Q2326/00 ,  C12Q2326/96 ,  Y10S435/805 ,  Y10S436/904 ,  Y10T436/206664

摘要： A quantitative analysis film comprising a reagent layer comprising a cationic dye-forming color indicator for detecting hydrogen peroxide and an anionic polymer. By the use of such a color indicator and an anionic polymer in combination, a cationic dye is formed; the formed cationic dye is then bound and fixed to the anionic polymer to provide an intensified color. A method for the colorimetric assay of hydrogen peroxide or a precursor thereof with high sensitivity and high efficiency is also disclosed.

摘要翻译： 一种定量分析膜，其包含试剂层，其包含用于检测过氧化氢的阳离子染料形成颜色指示剂和阴离子聚合物。 通过使用这种颜色指示剂和阴离子聚合物组合，形成阳离子染料; 然后将形成的阳离子染料结合并固定到阴离子聚合物以提供增强的颜色。 还公开了高灵敏度和高效率的过氧化氢或其前体的比色测定方法。

公开(公告)号：US4753531A

公开(公告)日：1988-06-28

申请号：US873284

申请日：1986-06-04

申请人： Nobuo Hiratsuka ,  Asaji Kondo

发明人： Nobuo Hiratsuka ,  Asaji Kondo

IPC分类号： B01L3/00 ,  G01N21/78 ,  G01N21/01 ,  G01N1/10

CPC分类号： B01L3/5023 ,  G01N21/78 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0481

摘要： An integral flat container type analytical instrument for use in the quantitative analysis of an analyte present in a liquid sample which has a reaction chamber and an observation chamber associated with each other so as to allow a liquid to flow therebetween characterized in that: at least part of the wall of said reaction chamber is made of a flexible material; said observation chamber is provided with wall parts which are made of a transparent material and opposed to each other in such a manner that their relative positions are fixed to provide a given light path; and no partition wall preventing the free flow of the liquid substantially exists between said reaction chamber and said observation chamber. A method for optical measurement of an analyte in a liquid sample utilizing said analytical instrument is also disclosed.

摘要翻译： 一种用于定量分析存在于液体样品中的分析仪的一体式扁平容器型分析仪器，其具有彼此相关联的反应室和观察室，以使液体在其间流动，其特征在于：至少部分 所述反应室的壁由柔性材料制成; 所述观察室设置有由透明材料制成并且彼此相对的壁部分，使得它们的相对位置被固定以提供给定的光路; 并且在所述反应室和所述观察室之间基本上不存在防止液体自由流动的分隔壁。 还公开了利用所述分析仪器对液体样品中的分析物进行光学测量的方法。

公开(公告)号：US4270920A

公开(公告)日：1981-06-02

申请号：US71618

申请日：1979-08-31

申请人： Asaji Kondo ,  Fuminori Arai ,  Masao Kitajima

发明人： Asaji Kondo ,  Fuminori Arai ,  Masao Kitajima

IPC分类号： G01N31/22 ,  G01N33/52 ,  G01N33/62 ,  G01N33/66 ,  G01N33/72

CPC分类号： G01N31/22 ,  G01N33/525

摘要： An integrated material for chemical analysis comprising three or more reagent layer units on a support and a porous spreading layer, said units containing reagents for different chemical analyses and being proximately arranged on a support such that approximately equal portions thereof are within a spreading circle defined by the diffusion of a liquid sample as it passes through the porous spreading layer, and a method for analyzing blood using the same.

摘要翻译： 一种用于化学分析的综合材料，其包括在支撑体上的三个或更多个试剂层单元和多孔扩散层，所述单元包含用于不同化学分析的试剂，并且近似地布置在支撑体上，使得其大致相等的部分在由 液体样品在通过多孔扩散层时的扩散，以及使用其分析血液的方法。

公开(公告)号：US4256693A

公开(公告)日：1981-03-17

申请号：US46125

申请日：1979-06-06

申请人： Asaji Kondo ,  Masao Kitajima

发明人： Asaji Kondo ,  Masao Kitajima

IPC分类号： G01N21/78 ,  G01N31/22 ,  G01N33/52 ,  G01N33/48 ,  G01N33/06

CPC分类号： G01N33/525 ,  Y10S435/805

摘要： A multilayered integral chemical analysis element for the blood comprising a filter layer capable of removing formed components from the blood, a water-proof layer having at least one small opening therein, a porous spreading layer and a reagent layer laminated in this order.

摘要翻译： 一种用于血液的多层整体化学分析元件，包括能够从血液中除去形成的成分的过滤层，其中具有至少一个小开口的防水层，多孔扩散层和试剂层。

公开(公告)号：US4255384A

公开(公告)日：1981-03-10

申请号：US66363

申请日：1979-08-14

申请人： Masao Kitajima ,  Fuminori Arai ,  Asaji Kondo

发明人： Masao Kitajima ,  Fuminori Arai ,  Asaji Kondo

IPC分类号： G01N33/52 ,  G01N31/22

CPC分类号： G01N33/525

摘要： An integral multilayered element for the chemical analysis of the blood comprising a combination of a porous spreading layer, a reagent layer, and a radiation-blocking layer, said radiation-blocking layer being water-permeable and containing at least one elemental metal or an alloy thereof.

摘要翻译： 一种用于血液化学分析的整体多层元件，包括多孔扩散层，试剂层和辐射阻挡层的组合，所述辐射阻挡层是透水性的并且包含至少一种元素金属或合金 其中。

公开(公告)号：US5004685A

公开(公告)日：1991-04-02

申请号：US86514

申请日：1987-08-18

申请人： Fuminori Arai ,  Asaji Kondo

发明人： Fuminori Arai ,  Asaji Kondo

IPC分类号： G01N33/50 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/30 ,  G01N31/22 ,  G01N33/52

CPC分类号： G01N33/525 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/96 ,  Y10S435/805 ,  Y10S436/81 ,  Y10S436/904

摘要： A dry-type multilayer analytical element containing a color reagent composition comprising an oxidase specifically reaction with an analyte or derived therefrom to produce hydrogen peroxide, hydrogen peroxide-decomposing enzyme, an alkali metal salt of ferrocyanide and a chromogen which is separated from the hydrogen peroxide-decomposing enzyme, the chromogen being incorporated in a layer nearer to the transparent support of the analytical element than the layer containing the hydrogen peroxide-decomposing enzyme. The analytical element exhibits excellent coloration, color stability, and accuracy and the results are minimally influenced by the presence of hemoglobin in the sample.

摘要翻译： 一种干型多层分析元件，其含有显色试剂组合物，其包含氧化酶与分析物特异性反应或由其衍生产生过氧化氢，过氧化氢分解酶，亚铁氰化物碱金属盐和与过氧化氢分离的色原体 分解酶，色原子被结合在比含有过氧化氢分解酶的层更接近分析元件的透明载体的层中。 分析元素显示出优异的着色，颜色稳定性和准确性，并且结果最小程度地受样品中血红蛋白的存在的影响。

公开(公告)号：US4981805A

公开(公告)日：1991-01-01

申请号：US682478

申请日：1984-12-17

申请人： Kenichiro Yazawa ,  Masao Kitajima ,  Asaji Kondo

发明人： Kenichiro Yazawa ,  Masao Kitajima ,  Asaji Kondo

IPC分类号： G01N1/28 ,  B01L3/00 ,  G01N21/78 ,  G01N21/86 ,  G01N33/48 ,  G01N33/52 ,  G02B21/34

CPC分类号： B01L3/508 ,  G01N21/8483 ,  G01N33/525 ,  G02B21/34 ,  B01L2200/142 ,  B01L2300/041 ,  B01L2300/048 ,  B01L2300/069 ,  B01L2300/0822 ,  B01L2400/0406

摘要： A method of using a chemical analysis slide comprising a slide frame and a sheet-like or film-like chemical analysis element for colorimetric determination of a specific component in an aqueous liquid sample, which comprisesapplying an aqueous liquid sample to the chemical analysis element, andcovering the upper face of the side of the slide frame to which the sample is applied with a sheet-like, plate-like or block or brick-like cover to thereby ensure a space between the chemical analysis element and the cover and to prevent evaporation of water or to control evaporation rate of water.

摘要翻译： 一种使用包括滑动框架和片状或膜状化学分析元件的化学分析载玻片的方法，用于比色测定水性液体样品中的特定组分，其包括将水性液体样品施加到化学分析元件， 并且将覆盖样品的滑动框架的侧面的上表面覆盖有片状，板状或块状或砖状的盖，​​从而确保化学分析元件与盖之间的空间，并防止 蒸发水或控制水的蒸发速率。