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    Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity
    1.
    发明授权
    Recombinant streptavidin-metallothionein chimeric protein having biological recognition specificity 失效
    具有生物识别特异性的重组链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白

    公开(公告)号：US06391590B1

    公开(公告)日：2002-05-21

    申请号：US07780717

    申请日：1991-10-21

    申请人： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    发明人： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    IPC分类号： C12N1562

    CPC分类号： C07H21/04 ,  C07K14/36 ,  C07K14/825 ,  C07K2319/00 ,  C07K2319/22

    摘要： Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

    摘要翻译： 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。

    Streptavidin proteins
    2.
    发明授权
    Streptavidin proteins 失效
    链霉亲和素蛋白

    公开(公告)号：US07179618B2

    公开(公告)日：2007-02-20

    申请号：US10285876

    申请日：2002-11-01

    申请人： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    发明人： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

    IPC分类号： C12N1/20 ,  C12N15/00 ,  C07K1/00 ,  C12P21/06

    CPC分类号： C07H21/04 ,  C07K14/36 ,  C07K14/825 ,  C07K2319/00 ,  C07K2319/22

    摘要： Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

    摘要翻译： 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。

    Fluorescent conjugates for analysis of molecules and cells
    5.
    发明授权
    Fluorescent conjugates for analysis of molecules and cells 失效
    用于分析和细胞分析的荧光偶联物

    公开(公告)号：US4859582A

    公开(公告)日：1989-08-22

    申请号：US701324

    申请日：1985-02-13

    申请人： Lubert Stryer ,  Alexander N. Glazer ,  Vernon T. Oi

    发明人： Lubert Stryer ,  Alexander N. Glazer ,  Vernon T. Oi

    IPC分类号： G01N33/533

    CPC分类号： G01N33/533 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S530/807

    摘要： Sensitive detection techniques and compositions for such techniques are provided by employing fluorescent proteins having bilin prosthetic groups as labels. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjunction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.

    摘要翻译： 通过使用具有胆汁假体基团的荧光蛋白作为标记来提供这种技术的敏感检测技术和组合物。 含有蛋白质的蛋白质可以与配体或受体结合，用于涉及配体 - 受体结合的系统，用于配体和受体的分析，检测或分离。 特别地，一种或多种含胆红蛋白的蛋白质可以用作彼此结合的标记或用于定义天然存在的聚集子集的其它荧光剂。 细胞。

    Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display
    6.
    发明授权
    Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display 有权
    多功能重组藻胆蛋白基荧光结构和phycobilisome显示

    公开(公告)号：US07176000B2

    公开(公告)日：2007-02-13

    申请号：US10617012

    申请日：2003-07-10

    申请人： Alexander N. Glazer ,  Yuping Cai

    发明人： Alexander N. Glazer ,  Yuping Cai

    IPC分类号： C12N15/09 ,  C12N1/20 ,  C07K14/00

    CPC分类号： C07K14/415 ,  C07K2319/00 ,  Y10S435/822

    摘要： The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein. including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.

    摘要翻译： 本发明提供了多功能融合构建体，其迅速并入大分子结构例如藻糖酵母中，使得融合蛋白彼此分离并且不能自相关。 本发明提供了在寡聚藻胆蛋白上显示功能性多肽结构域的方法和组合物。 包括包含功能性显示结构域的融合蛋白和掺入功能性寡聚藻胆蛋白的功能性藻胆蛋白结构域。 融合蛋白提供新的特异性标记试剂。

    Engineering of living cells for the expression of holo-phycobiliprotein-based constructs
    7.
    发明授权
    Engineering of living cells for the expression of holo-phycobiliprotein-based constructs 有权
    用于表达全脂蛋白脂蛋白的构建体的活细胞工程

    公开(公告)号：US06740507B2

    公开(公告)日：2004-05-25

    申请号：US09919486

    申请日：2001-07-31

    申请人： Alexander N. Glazer ,  Aaron J. Tooley ,  Yuping Cai

    发明人： Alexander N. Glazer ,  Aaron J. Tooley ,  Yuping Cai

    IPC分类号： C12P2104

    CPC分类号： C12N9/0095 ,  C07K14/43595 ,  C07K2319/21 ,  C07K2319/60 ,  C12N9/88 ,  C12N15/52 ,  C12N15/62

    摘要： Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.

    摘要翻译： 描述表达荧光全血蓝蛋白融合蛋白的重组细胞及其使用方法。 所述细胞包含bilin，重组bi​​lin还原酶，包含相应的载脂蛋白结构域的融合蛋白的载脂蛋白 - 磷脂蛋白融合蛋白前体，以及组分反应形成全血蓝蛋白融合蛋白的重组体藻胆蛋白结构域 - 胆固醇裂解酶 。 还描述了基于全藻蛋白脂蛋白的转录报道细胞和测定法，其中细胞条件地表达异源细胞，荧光，第一全息型蛋白脂蛋白结构域。

    Determination of nucleotide sequence variations through limited primer extension
    10.
    发明授权
    Determination of nucleotide sequence variations through limited primer extension 失效
    通过有限的引物延伸测定核苷酸序列变异

    公开(公告)号：US06355433B1

    公开(公告)日：2002-03-12

    申请号：US09586125

    申请日：2000-06-02

    申请人： Hua Xu ,  Alexander N. Glazer

    发明人： Hua Xu ,  Alexander N. Glazer

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6858 ,  C12Q2537/157 ,  C12Q2535/125

    摘要： The present invention provides methods and kits for determining the identity of a nucleotide at a variant site in a target nucleic acid of interest, including, for example, point mutations and single nucleotide polymorphisms. The methods involve conducting template-dependent extension reactions in the presence of a mixture of nucleotides that include at least one labeled extendible nucleotide and at least one labeled non-extendible nucleotide that are selected to be complementary to the nucleotides that potentially occupy the variant site. The particular labeled nucleotide incorporated into the extension products is characteristic of the nucleotide at the variant site. In addition to their utility in analyzing point mutations and single nucleotide polymorphisms, the methods and kits of the invention have utility in a variety of other applications in which specific nucleotide sequence information is of value, including, for example, paternity disputes, prenatal testing, forensic analysis and detection of pathogens.

    摘要翻译： 本发明提供用于确定感兴趣的靶核酸中的变体位点处的核苷酸的身份的方法和试剂盒，包括例如点突变和单核苷酸多态性。 所述方法包括在核苷酸混合物存在下进行模板依赖性延伸反应，所述核苷酸混合物包括至少一个标记的可延伸核苷酸和至少一个标记的不可延伸核苷酸，其被选择为与可能占据该变体位点的核苷酸互补。 掺入延伸产物中的特定标记核苷酸是变体位点核苷酸的特征。 除了其在分析点突变和单核苷酸多态性中的用途之外，本发明的方法和试剂盒可用于其中特异性核苷酸序列信息有价值的多种其它应用，包括例如亲子争议，产前检验， 法医分析和病原体检测。