公开(公告)号：US20100137568A1

公开(公告)日：2010-06-03

申请号：US12450443

申请日：2008-03-26

申请人： Oded Shoseyov ,  Ziv Shani ,  Miron Abramson ,  Noga Barimboim ,  Mara Dekel ,  Shaul Lapidot

发明人： Oded Shoseyov ,  Ziv Shani ,  Miron Abramson ,  Noga Barimboim ,  Mara Dekel ,  Shaul Lapidot

IPC分类号： C12N15/82 ,  C07C27/04 ,  C07G1/00

CPC分类号： C12N15/8246 ,  C12N9/0006 ,  C12N9/1055 ,  C12N9/14 ,  C12P7/10 ,  C12Y101/99018 ,  Y02E50/16

摘要： The present invention provides a bio-fuel, wood or other product, such as a paper, textile or yarn product. The product can contain material from a transgenic plant over-expressing a nucleic acid molecule encoding an enzyme that causes the plant cell wall to be more water soluble than the wild type.

摘要翻译： 本发明提供了生物燃料，木材或其他产品，例如纸，纺织品或纱线产品。 产物可以含有来自转基因植物的材料，其过表达编码使得植物细胞壁比野生型更可溶于水的酶的核酸分子。

公开(公告)号：US10011846B2

公开(公告)日：2018-07-03

申请号：US12450443

申请日：2008-03-26

申请人： Oded Shoseyov ,  Ziv Shani ,  Miron Abramson

发明人： Oded Shoseyov ,  Ziv Shani ,  Miron Abramson

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N9/04 ,  C12N9/10 ,  C12N9/14 ,  C12P7/10

CPC分类号： C12N15/8246 ,  C12N9/0006 ,  C12N9/1055 ,  C12N9/14 ,  C12P7/10 ,  C12Y101/99018 ,  Y02E50/16

摘要： The present invention provides a bio-fuel, wood or other product, such as a paper, textile or yarn product. The product can contain material from a transgenic plant over-expressing a nucleic acid molecule encoding an enzyme that causes the plant cell wall to be more water soluble than the wild type.

公开(公告)号：US20100212050A1

公开(公告)日：2010-08-19

申请号：US12593620

申请日：2008-03-26

申请人： Oded Shoseyov ,  Uzi Kafkafi ,  Miron Abramson ,  Ziv Shani

发明人： Oded Shoseyov ,  Uzi Kafkafi ,  Miron Abramson ,  Ziv Shani

IPC分类号： A01H5/12 ,  A01H5/00

CPC分类号： C12N15/8273 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention relates to compositions and methods for over-expressing pectin methylesterases (PMEs), particularly type 1 PMEs (PME1s), in plants while maintaining growth as compated to the wild type. The plants of the invention have improved properties compared to the wild type, particularly improved resistance to salinity.

摘要翻译： 本发明涉及在植物中过度表达果胶甲酯酶（PME），特别是1型PME（PME1）的组合物和方法，同时保持与野生型相符的生长。 与野生型相比，本发明的植物具有改善的特性，特别是改善的耐盐性。

公开(公告)号：US20130227724A1

公开(公告)日：2013-08-29

申请号：US13883046

申请日：2011-11-03

申请人： Miron Abramson ,  Ziv Shani ,  Oded Shoseyov

发明人： Miron Abramson ,  Ziv Shani ,  Oded Shoseyov

IPC分类号： C12N15/82

CPC分类号： C12N15/8255 ,  C12N9/18 ,  C12N15/8243 ,  C12N15/8246 ,  C12Y301/01072

摘要： A method of engineering a plant having reduced acetylation in a cell wall is disclosed. The method comprising expressing in the plant cell wall at least one isolated heterologous polynucleotide encoding an acetylxylan esterase (AXE) enzyme under the transcriptional control of a developmentally regulated promoter specifically active in the plant cell wall upon secondary cell wall deposit, thereby engineering the plant having reduced acetylation in the cell wall.

摘要翻译： 公开了一种在细胞壁中工程化乙酰化减少的植物的方法。 所述方法包括在植物细胞壁上表达至少一种编码乙酰木聚糖酯酶（AX）酶的分离的异源多核苷酸，其在二次细胞壁沉积物上在植物细胞壁中特异性活化的发育调节启动子的转录控制下，由此工程化具有 减少细胞壁中的乙酰化。

公开(公告)号：US06323023B1

公开(公告)日：2001-11-27

申请号：US09325274

申请日：1999-06-03

申请人： Oded Shoseyov ,  Ziv Shani

发明人： Oded Shoseyov ,  Ziv Shani

IPC分类号： C12N1529

CPC分类号： C12N9/2437 ,  A01K2217/05 ,  C07K14/33 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8229 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8261 ,  C12N15/8271 ,  C12P19/04 ,  C12Y302/01004 ,  Y02A40/146

摘要： The present invention discloses genetically engineered plants which display altered structure or morphology. The transgenic plants express a cell wall modulation transgene or gene construct that results in the altered structure or morphology. The altered structure or morphology can be associated with, for example, altered biomass, growth, yield, greater or less resistance to biodegradation, more or less digestible to ruminants, altered cellulose content, larger leaves/normal hypocotyls or smaller leaves/longer hypocotyls, etc. compared to a non-transgenic plant of the same species. The cell wall modulation transgene can be any cellulose binding domain, a cellulose binding protein, or a cell wall modifying protein or enzyme such as endoxyloglucan transferase, xyloglucan endo-transglycosylase, an expansin, cellulose synthase, or a novel isolated endo-1,4-&bgr;-glucanase of Arabidopsis thaliana. The invention also discloses transgenic plants containing a gene construct comprising a promoter operably linked to the cell wall modulation protein or polypeptide gene and may further comprise a sequence encoding a secretion signal peptide. In particular, the invention discloses transgenic plants containing a gene construct comprising the cel1 promoter, operably linked to the cel1 signal peptide and any cellulose binding domain. Methods for modulating plant growth by transgenic expression of a cell wall modulating protein or polypeptide are also disclosed. The present invention also discloses a novel, isolated Arabidopsis thaliana endo-1,4-&bgr;-glucanase gene (cel1), its promoter (cel1 promoter) and polypeptide (Cel1) and recombinant nucleic acid vectors containing the cel1 gene with or without a secretion signal peptide sequence and/or the cel1 promoter.

摘要翻译： 本发明公开了显示改变的结构或形态的遗传工程植物。 转基因植物表达导致改变的结构或形态的细胞壁调节转基因或基因构建体。 改变的结构或形态可以与例如改变的生物量，生长，产量，更大或更小的抗生物降解性，或多或少可消化的反刍动物，纤维素含量变化，较大的叶/正常的下胚轴或更小的叶/更长的下胚轴， 与相同物种的非转基因植物相比。 细胞壁调节转基因可以是任何纤维素结合结构域，纤维素结合蛋白或细胞壁修饰蛋白或酶如内切葡聚糖转移酶，木葡聚糖内转葡糖基酶，扩展蛋白，纤维素合成酶或新型分离的内-1,4- 拟南芥的β-葡聚糖酶。 本发明还公开了含有可操作地连接于细胞壁调节蛋白或多肽基因的启动子的基因构建体的转基因植物，并且还可以包含编码分泌信号肽的序列。 特别地，本发明公开了含有可操作地连接到cel1信号肽和任何纤维素结合结构域的包含cel1启动子的基因构建体的转基因植物。 还公开了通过细胞壁调节蛋白或多肽的转基因表达调节植物生长的方法。 本发明还公开了一种新颖的分离拟南芥内切-1,4-β-葡聚糖酶基因（cel1），其启动子（cel1启动子）和多肽（Cel1）以及含有或不具有分泌物的cel1基因的重组核酸载体 信号肽序列和/或cel1启动子。

公开(公告)号：US6005092A

公开(公告)日：1999-12-21

申请号：US6636

申请日：1998-01-13

申请人： Oded Shoseyov ,  Ziv Shani

发明人： Oded Shoseyov ,  Ziv Shani

IPC分类号： A01H5/00 ,  A01H5/10 ,  C07K14/33 ,  C12N20060101 ,  C12N5/04 ,  C12N5/10 ,  C12N5/14 ,  C12N9/00 ,  C12N9/36 ,  C12N9/42 ,  C12N15/09 ,  C12N15/14 ,  C12N15/29 ,  C12N15/31 ,  C12N15/63 ,  C12N15/82 ,  C12P19/04 ,  C12N15/56

CPC分类号： C12N9/2437 ,  C07K14/33 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8229 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8261 ,  C12N15/8271 ,  C12P19/04 ,  C12Y302/01004 ,  A01K2217/05

摘要： The present invention discloses genetically engineered plants which display altered structure or morphology. The transgenic plants express a cell wall modulation transgene or gene construct that results in the altered structure or morphology. The altered structure or morphology can be associated with, for example, altered biomass, growth, yield, greater or less resistance to biodegradation, more or less digestible to ruminants, altered cellulose content, larger leaves/normal hypocotyls or smaller leaves/longer hypocotyls, etc. compared to a non-transgenic plant of the same species. The cell wall modulation transgene can be any cellulose binding domain, a cellulose binding protein, or a cell wall modifying protein or enzyme such as endoxyloglucan transferase, xyloglucan endo-transglycosylase, an expansin, cellulose synthase, or a novel isolated endo-1,4-.beta.-glucanase of Arabidopsis thaliana. The invention also discloses transgenic plants containing a gene construct comprising a promoter operably linked to the cell wall modulation protein or polypeptide gene and may further comprise a sequence encoding a secretion signal peptide. In particular, the invention discloses transgenic plants containing a gene construct comprising the cel1 promoter, operably linked to the cel1 signal peptide and any cellulose binding domain. Methods for modulating plant growth by transgenic expression of a cell wall modulating protein or polypeptide are also disclosed. The present invention also discloses a novel, isolated Arabidopsis thaliana endo-1,4-.beta.-glucanase gene (cel1), its promoter (cel1 promoter) and polypeptide (Cel1) and recombinant nucleic acid vectors containing the cel1 gene with or without a secretion signal peptide sequence and/or the cel1 promoter.

摘要翻译： 本发明公开了显示改变的结构或形态的遗传工程植物。 转基因植物表达导致改变的结构或形态的细胞壁调节转基因或基因构建体。 改变的结构或形态可以与例如改变的生物量，生长，产量，更大或更小的抗生物降解性，或多或少可消化的反刍动物，纤维素含量变化，较大的叶/正常的下胚轴或更小的叶/更长的下胚轴， 与相同物种的非转基因植物相比。 细胞壁调节转基因可以是任何纤维素结合结构域，纤维素结合蛋白或细胞壁修饰蛋白或酶如内切葡聚糖转移酶，木葡聚糖内转葡糖基酶，扩展蛋白，纤维素合成酶或新型分离的内-1,4- - 拟南芥的β-葡聚糖酶。 本发明还公开了含有可操作地连接于细胞壁调节蛋白或多肽基因的启动子的基因构建体的转基因植物，并且还可以包含编码分泌信号肽的序列。 特别地，本发明公开了含有可操作地连接到cel1信号肽和任何纤维素结合结构域的包含cel1启动子的基因构建体的转基因植物。 还公开了通过细胞壁调节蛋白或多肽的转基因表达调节植物生长的方法。 本发明还公开了一种新颖的分离的拟南芥内-4,4-β-葡聚糖酶基因（cel1），其启动子（cel1启动子）和多肽（Cel1）和含有或不具有分泌物的cel1基因的重组核酸载体 信号肽序列和/或cel1启动子。

公开(公告)号：US06331416B1

公开(公告)日：2001-12-18

申请号：US09329234

申请日：1999-06-10

申请人： Ziv Shani ,  Oded Shoseyov

发明人： Ziv Shani ,  Oded Shoseyov

IPC分类号： C07K1900

CPC分类号： C07K14/315 ,  C07K2319/035 ,  C07K2319/04 ,  C07K2319/50 ,  C07K2319/705 ,  C12N15/62 ,  C12N15/8257

摘要： A process of expressing a recombinant protein in a plant and of isolating the recombinant protein from the plant, the process is effected by (a) providing a plant, a plant derived tissue or cultured plant cells expressing a fusion protein including the recombinant protein and a cellulose binding peptide being fused thereto, the fusion protein being compartmentalized within cells of the plant, plant derived tissue or cultured plant cells, so as to be sequestered from cell walls of the cells of the plant, plant derived tissue or cultured plant cells; (b) homogenizing the plant, plant derived tissue or cultured plant cells, so as to bring into contact the fusion protein with a cellulosic matter of the plant, plant derived tissue or cultured plant cells, to thereby effect affinity binding of the fusion protein via the cellulose binding peptide to the cellulosic matter, thereby obtaining a fusion protein cellulosic matter complex; and (c) isolating the fusion protein cellulosic matter complex.

摘要翻译： 一种在植物中表达重组蛋白质并从植物中分离重组蛋白质的方法，该方法通过（a）提供植物，植物衍生的组织或表达包含重组蛋白质的融合蛋白的培养的植物细胞，以及 融合蛋白被分隔在植物的细胞内，植物衍生的组织或培养的植物细胞中，以便从植物，植物衍生的组织或培养的植物细胞的细胞壁隔离; （b）使植物，植物衍生的组织或培养的植物细胞均质化，以使融合蛋白与植物的纤维素物质，植物衍生的组织或培养的植物细胞接触，从而通过融合蛋白经由 纤维素结合肽与纤维素物质结合，从而获得融合蛋白纤维素复合物; 和（c）分离融合蛋白纤维素复合物。

公开(公告)号：US06184440B2

公开(公告)日：2001-02-06

申请号：US09006632

申请日：1998-01-13

申请人： Oded Shoseyov ,  Ziv Shani ,  Etai Shpigel

发明人： Oded Shoseyov ,  Ziv Shani ,  Etai Shpigel

IPC分类号： C12N504

CPC分类号： C12N9/2437 ,  A01K2217/05 ,  C07K14/33 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8229 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8261 ,  C12N15/8271 ,  C12P19/04 ,  C12Y302/01004 ,  Y02A40/146

摘要： The present invention discloses genetically engineered plants which display altered structure or morphology. The transgenic plants express a cell wall modulation transgene or gene construct that results in the altered structure or morphology. The altered structure or Morphology can be associated with, for example, altered biomass, growth, yield, greater or less resistance to biodegradation, more or less digestible to ruminants, altered cellulose content, larger leaves/normal hypocotyls or smaller leaves/longer hypocotyls, etc. compared to a non-transgenic plant of the same species. The cell wall modulation transgene can be any cellulose binding domain, a cellulose binding protein, or a cell wall modifying protein or enzyme such as endoxyloglucan transferase, xyloglucan endo-transglycosylase, an expansin, cellulose synthase, or a novel isolated endo-1,4-&bgr;-glucanase of Arabidopsis thaliana. The invention also discloses transgenic plants containing a gene construct comprising a promoter operably linked to the cell wall modulation protein or polypeptide gene and may further comprise a sequence encoding a secretion signal peptide. In particular, the invention discloses transgenic plants containing a gene construct comprising the cel1 promoter, operably linked to the cel1 signal peptide and any cellulose binding domain. Methods for modulating plant growth by transgenic expression of a cell wall modulating protein or polypeptide are also disclosed. The present invention also discloses a novel, isolated Arabidopsis thaliana endo-1,4-&bgr;-glucanase gene (cel1), its promoter (cel1 promoter) and polypeptide (Cel1) and recombinant nucleic acid vectors containing the cel1 gene with or without a secretion signal peptide sequence and/or the cel1 promoter.

摘要翻译： 本发明公开了显示改变的结构或形态的遗传工程植物。 转基因植物表达导致改变的结构或形态的细胞壁调节转基因或基因构建体。 改变的结构或形态可以与例如改变的生物量，生长，产量，更大或更小的抗生物降解性，或多或少可消化的反刍动物，改变的纤维素含量，更大的叶/正常下胚轴或更小的叶/更长的下胚轴， 与相同物种的非转基因植物相比。 细胞壁调节转基因可以是任何纤维素结合结构域，纤维素结合蛋白或细胞壁修饰蛋白或酶如内切葡聚糖转移酶，木葡聚糖内转葡糖基酶，扩展蛋白，纤维素合成酶或新型分离的内-1,4- 拟南芥的β-葡聚糖酶。 本发明还公开了含有可操作地连接于细胞壁调节蛋白或多肽基因的启动子的基因构建体的转基因植物，并且还可以包含编码分泌信号肽的序列。 特别地，本发明公开了含有可操作地连接到cel1信号肽和任何纤维素结合结构域的包含cel1启动子的基因构建体的转基因植物。 还公开了通过细胞壁调节蛋白或多肽的转基因表达调节植物生长的方法。 本发明还公开了一种新颖的分离拟南芥内切-1,4-β-葡聚糖酶基因（cel1），其启动子（cel1启动子）和多肽（Cel1）以及含有或不具有分泌物的cel1基因的重组核酸载体 信号肽序列和/或cel1启动子。

公开(公告)号：US08013211B2

公开(公告)日：2011-09-06

申请号：US11994949

申请日：2006-07-05

申请人： Ziv Shani

发明人： Ziv Shani

IPC分类号： C12N15/82 ,  C12N15/13 ,  C12N5/06 ,  C12N5/10 ,  A01N63/00 ,  A01N65/00 ,  A61K48/00

CPC分类号： C12N15/8207 ,  C12N15/8202

摘要： The invention relates to stinging cells or isolated capsules and to their use in compositions and methods for efficient delivery of biologically active agents into a plant cell or plant tissue. The biologically active agent to be delivered by the methods of the present invention is selected from a nucleic acid, a peptide, a polypeptide, a plant hormone, an enzyme, an herbicidal agent, an anti-viral agent, an anti-bacterial agent and an anti-fungal agent. Particularly, the invention is related to compositions and methods for the efficient transformation of polynucleotide construct into a plant cell or tissue, to obtain transgenic plants.

摘要翻译： 本发明涉及刺激细胞或分离的胶囊，以及它们在将生物活性剂有效递送到植物细胞或植物组织的组合物和方法中的用途。 通过本发明的方法递送的生物活性剂选自核酸，肽，多肽，植物激素，酶，除草剂，抗病毒剂，抗菌剂和 一种抗真菌剂。 特别地，本发明涉及将多核苷酸构建体有效转化为植物细胞或组织以获得转基因植物的组合物和方法。

公开(公告)号：US20090144855A1

公开(公告)日：2009-06-04

申请号：US11994949

申请日：2006-07-05

申请人： Ziv Shani

发明人： Ziv Shani

IPC分类号： C12N15/11 ,  C12N5/04 ,  C12N5/10 ,  C12Q1/02 ,  C12Q1/68

CPC分类号： C12N15/8207 ,  C12N15/8202

摘要： The invention relates to stinging cells or isolated capsules and to their use in compositions and methods for efficient delivery of biologically active agents into a plant cell or plant tissue. The biologically active agent to be delivered by the methods of the present invention is selected from a nucleic acid, a peptide, a polypeptide, a plant hormone, an enzyme, an herbicidal agent, an anti-viral agent, an anti-bacterial agent and an anti-fungal agent. Particularly, the invention is related to compositions and methods for the efficient transformation of polynucleotide construct into a plant cell or tissue, to obtain transgenic plants.

摘要翻译： 本发明涉及刺激细胞或分离的胶囊，以及它们在将生物活性剂有效递送到植物细胞或植物组织的组合物和方法中的用途。 通过本发明的方法递送的生物活性剂选自核酸，肽，多肽，植物激素，酶，除草剂，抗病毒剂，抗菌剂和 一种抗真菌剂。 特别地，本发明涉及将多核苷酸构建体有效转化为植物细胞或组织以获得转基因植物的组合物和方法。