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    Method of preparing fluoroaromatic compounds
    1.
    发明授权
    Method of preparing fluoroaromatic compounds 有权
    氟代芳族化合物的制备方法

    公开(公告)号：US6008407A

    公开(公告)日：1999-12-28

    申请号：US185171

    申请日：1998-11-03

    申请人： Oleg Matveyevich Nefedov ,  Nikolay Vasiljcvich Volchkov ,  Mariya Borisovna Lipkind ,  Ho Seong Lee ,  Young Jun Park ,  Min Hwan Kim

    发明人： Oleg Matveyevich Nefedov ,  Nikolay Vasiljcvich Volchkov ,  Mariya Borisovna Lipkind ,  Ho Seong Lee ,  Young Jun Park ,  Min Hwan Kim

    IPC分类号： C07C17/25 ,  C07C17/30 ,  C07C25/13 ,  C07C51/58 ,  C07C51/16

    CPC分类号： C07C17/30 ,  C07C17/25 ,  C07C25/13 ,  C07C51/093 ,  C07C51/58

    摘要： The present invention provides a method of preparing ortho-difluorobenzene derivatives, which comprises (a) providing a mixture of cyclohexenes by reacting chlorotrifluoroethylene (CTFE) and 1.3-diene in a flow reactor and distilling the resultant, and (b) dehydrohalogenating the mixture of cyclohexenes with a phase transition catalyst in :he presence of alkali metal hydroxide at temperature range of 40 to 150.degree. C. without using any organic solvent. The distillate having low boiling point, which is obtained during distillation of the resultant, is recycled into the flow reactor. The present invention also provides a method of preparing 2-chloro-4,5-difluorobenzoic acid, which comprises (a) providing a mixture of 4-chloro-1-methyl-4,5,5-trifluorocyclohexene and 5-chloro-1-methyl-4,4,5-trifluorocyclohexene by reacting chlorotrifluoroethylene (CTFE) and isoprene and distilling the resultant. (b) dehydrohalogenating said mixture in the presence of alkali metal hydroxide and a phase transition catalyst to form 3,4-difluorotoluene, to reacting said 3,4-difluorotoluene with chlorine gas without using any organic solvent to form 2-chloro-4,5-difluorotoluene, (d) photo-reacting said 2-chloro-4,5-difluorotoluene with chlorine gas under a lighting mercury lamp without using any organic solvent to form 2-chloro-4,5-difluorobenzotrichloride, and (e) reacting said 2-chloro-4,5-difluorobenzotrichloride with aqueous acid solution without using any organic solvent. The present invention also provides a method of preparing 2-chloro-4,5-difluorobenzoyl chloride by reacting the 2-chloro-4,5-difluorobenzotrichloride of step (e) above with zinc oxide.

    摘要翻译： 本发明提供了一种制备邻二氟苯衍生物的方法，其包括（a）通过在流动反应器中使氯三氟乙烯（CTFE）和1,3-二烯反应提供环己烯的混合物并蒸馏所得物，和（b）脱卤化氢 具有相变催化剂的环己烯：在不使用任何有机溶剂的情况下，在40至150℃的温度范围内存在碱金属氢氧化物。 在蒸馏过程中获得的具有低沸点的馏出物被循环到流动反应器中。 本发明还提供了制备2-氯-4,5-二氟苯甲酸的方法，其包括（a）提供4-氯-1-甲基-4,5,5-三氟环己烯和5-氯-1 - 甲基-4,4,5-三氟环己烯，通过氯三氟乙烯（CTFE）和异戊二烯反应并蒸馏所得物。 （b）在碱金属氢氧化物和相变催化剂的存在下将所述混合物脱卤化氢以形成3,4-二氟甲苯，使所述3,4-二氟甲苯与氯气反应，而不使用任何有机溶剂形成2-氯-4， （d）在照明汞灯下使所述2-氯-4,5-二氟甲苯与氯气进行光反应，而不使用任何有机溶剂形成2-氯-4,5-二氟三氯甲苯，和（e）使 所述2-氯-4,5-二氟三氯甲烷与含水酸溶液不使用任何有机溶剂。 本发明还提供通过使上述步骤（e）的2-氯-4,5-二氟三氯甲苯与氧化锌反应来制备2-氯-4,5-二氟苯甲酰氯的方法。

    METHOD FOR DETECTING NUCLEIC ACIDS BY SIMULTANEOUS ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND SIGNAL PROBE
    3.
    发明申请
    METHOD FOR DETECTING NUCLEIC ACIDS BY SIMULTANEOUS ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND SIGNAL PROBE 审中-公开
    通过同位素放射核酸和信号探针检测核酸的方法

    公开(公告)号：US20100311058A1

    公开(公告)日：2010-12-09

    申请号：US12745544

    申请日：2008-04-24

    申请人： Min Hwan Kim ,  Sook Lee ,  Un Ok Kim ,  Ji Won Jeong ,  Joo Hee Lee

    发明人： Min Hwan Kim ,  Sook Lee ,  Un Ok Kim ,  Ji Won Jeong ,  Joo Hee Lee

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6853 ,  C12Q2531/119 ,  C12Q2525/121 ,  C12Q2525/161

    摘要： The present invention relates to a method for detecting target nucleic acids by simultaneous isothermal amplification of the target nucleic acids and a signal probe 5 using an external primer set, a DNA-RNA-DNA hybrid primer set and a DNA-RNA-DNA hybrid signal probe. The method according to the present invention can be used to amplify target nucleic acids in a sample, rapid and exact manner without the risk of contamination compared to the conventional methods such as PCR, and it can simultaneously amplify target nucleic acid and a signal probe, so that it can 0 be applied to various genome projects, detection and identification of a pathogen, detection of gene modification producing a predetermined phenotype, detection of hereditary diseases or determination of sensibility to diseases, and estimation of gene expression. Thus, it is useful for molecular biological studies and disease diagnosis.

    摘要翻译： 本发明涉及通过使用外部引物组，DNA-RNA-DNA杂交引物组和DNA-RNA-DNA混合信号同时等温扩增靶核酸和信号探针5来检测靶核酸的方法 探测。 根据本发明的方法可以用于与常规方法如PCR相比以快速和准确的方式扩增靶核酸而没有污染的风险，并且可以同时扩增靶核酸和信号探针， 从而可以将其应用于各种基因组项目，病原体的检测和鉴定，产生预定表型的基因修饰的检测，遗传性疾病的检测或疾病的敏感性的测定以及基因表达的估计。 因此，它对分子生物学研究和疾病诊断有用。

    METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND METHOD FOR DETECTING NUCLEIC ACIDS USING SIMULTANEOUS ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND SIGNAL PROBE
    6.
    发明申请
    METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND METHOD FOR DETECTING NUCLEIC ACIDS USING SIMULTANEOUS ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND SIGNAL PROBE 审中-公开
    用于核酸的同位素放大的方法和使用核酸和信号探针的同时同位素放大来检测核酸的方法

    公开(公告)号：US20090130677A1

    公开(公告)日：2009-05-21

    申请号：US12090196

    申请日：2006-09-08

    申请人： Min Hwan Kim ,  Ji Won Jeong ,  Un Ok Kim

    发明人： Min Hwan Kim ,  Ji Won Jeong ,  Un Ok Kim

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6853 ,  C12Q1/6846 ,  C12Q1/6865 ,  C12Q2537/143 ,  C12Q2537/1376 ,  C12Q2521/101

    摘要： The present invention relates to a method for isothermal amplification of nucleic acids and a method for detecting nucleic acids, which comprises characterized in simultaneous isothermal amplification of nucleic acids and a signal probe to a method for isothermal amplification of target nucleic acids using an external primer set and RNA/DNA hybrid primer set, and a method for detecting amplification products by amplifying nucleic acids and a signal probe simultaneously using an external primer set, RNA-DNA hybrid primer set and DNA-RNA-DNA hybrid probe. The method according to the present invention is convenient compared with the conventional method, it is possible to amplify the target nucleic acids rapidly and exactly without a risk of contamination, and it can simultaneously amplify a signal probe, so that it can be applied to various genome project, such as detection and identification of a pathogen, detection of gene modification causing predetermined phenotype, detection of hereditary diseases or determination of sensibility to diseases, estimation of gene expression and apply to genome project, thus being useful for molecular biological studies and disease diagnosis.

    摘要翻译： 本发明涉及核酸的等温扩增方法和核酸检测方法，其特征在于将核酸同时等温扩增和信号探针用于使用外部引物组等位扩增靶核酸的方法 和RNA / DNA杂交引物组，以及通过使用外部引物组，RNA-DNA杂交引物组和DNA-RNA-DNA杂交探针同时扩增核酸和信号探针来检测扩增产物的方法。 根据本发明的方法与常规方法相比是方便的，可以快速准确地扩增靶核酸而没有污染的风险，并且可以同时放大信号探针，从而可以应用于各种 基因组计划，如病原体的检测和鉴定，检测导致预期表型的基因修饰，遗传性疾病的检测或疾病敏感性的测定，基因表达的估计和应用于基因组计划，因此可用于分子生物学研究和疾病 诊断。

    DMOS transistor having a high reliability and a method for fabricating the same
    7.
    发明授权
    DMOS transistor having a high reliability and a method for fabricating the same 有权
    具有高可靠性的DMOS晶体管及其制造方法

    公开(公告)号：US06252279B1

    公开(公告)日：2001-06-26

    申请号：US09376710

    申请日：1999-08-17

    申请人： Min Hwan Kim

    发明人： Min Hwan Kim

    IPC分类号： H01L2976

    CPC分类号： H01L29/7816 ,  H01L29/0619 ,  H01L29/0847 ,  H01L29/1095 ,  H01L29/402 ,  H01L29/41758

    摘要： A power DMOS transistor having an improved current driving capability and improved reliability is provided. A fabrication method thereof is also provided. The DMOS transistor includes a semiconductor substrate having a first conductivity type and a semiconductor region having a second conductivity type formed on the semiconductor substrate. A drain having a second conductivity type is formed on the semiconductor region. A high-concentration buried impurity layer having a second conductivity type is formed on the semiconductor region under the drain. A body region having a first conductivity type is formed in the semiconductor substrate spaced a predetermined distance from the semiconductor region. A source having a second conductivity type is formed on the body region. A gate electrode is formed on the semiconductor substrate having a gate insulative film formed thereon. A source electrode and a drain electrode coupled respectively to the source and the drain are formed on the resultant structure.

    摘要翻译： 提供了具有改进的电流驱动能力和改进的可靠性的功率DMOS晶体管。 还提供了其制造方法。 DMOS晶体管包括具有第一导电类型的半导体衬底和形成在半导体衬底上的具有第二导电类型的半导体区域。 在半导体区域上形成具有第二导电类型的漏极。 在漏极下面的半导体区域上形成具有第二导电类型的高浓度掩埋杂质层。 在与半导体区域隔开预定距离的半导体衬底中形成具有第一导电类型的体区。 在身体区域上形成具有第二导电类型的源。 在其上形成有栅极绝缘膜的半导体衬底上形成栅电极。 在所得结构上形成分别与源极和漏极耦合的源电极和漏电极。