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公开(公告)号：US08961904B2
公开(公告)日：2015-02-24
申请号：US13943322
申请日：2013-07-16
发明人： Zheng Xia , Yu Zhou , John Larsen , Guocheng Shao , Shane Peterson , Marjorie Faust
CPC分类号： G01N15/1404 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2200/10 , B01L2300/0627 , B01L2300/0816 , B01L2300/0864 , B01L2300/0874 , B01L2300/0887 , B01L2400/0439 , B01L2400/0475 , C12N5/0612 , F04B19/006 , F04B43/043 , F04B43/095 , G01N15/1459 , G01N15/1484 , G01N2015/1006 , G01N2015/1409 , Y10T436/25
摘要： A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.
摘要翻译： 微流体芯片通过两步聚焦来定向和分离样品流体混合物中的组分，其中鞘液在一个方向上压缩样品输入通道中的样品流体混合物，使得样品流体混合物变成由鞘液界限的较窄流 并且通过使鞘液在另外的下游的第二方向上压缩样品流体混合物，使得组分被压缩并沿着选定的方向定向以通过各种方法进行识别和分离的单个文件形成中的询问室。 隔离机构利用设置在微流体芯片保持器上的外部堆叠的压电致动器组件或芯片上的压电致动器组件，使得致动器组件由电子信号触发，以驱动样品输入通道两侧的喷射室， 将采样输入通道中的选定分量喷射到输出通道之一中。
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公开(公告)号：US20170167966A1
公开(公告)日：2017-06-15
申请号：US15387034
申请日：2016-12-21
发明人： David Appleyard , Jeff Betthauser , Marjorie Faust , John Larsen , Guocheng Shao , Zheng Xia , Yu Zhou
IPC分类号： G01N15/14 , G01N21/01 , G01N33/483 , G01N1/44
CPC分类号： G01N15/1484 , B01L3/502761 , B01L2200/0636 , B01L2200/0652 , B01L2300/0627 , C12N13/00 , G01N1/44 , G01N15/1404 , G01N21/01 , G01N21/6486 , G01N33/4833 , G01N2015/1006 , G01N2015/1409 , G01N2015/1413
摘要： An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the micro-fluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.
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公开(公告)号：US20170052105A1
公开(公告)日：2017-02-23
申请号：US15033001
申请日：2014-06-18
发明人： David Appleyard , Jeff Betthauser , Marjorie Faust , John Larsen , Guocheng Shao , Zheng Xia , Yu Zhou
CPC分类号： G01N15/1484 , B01L3/502761 , B01L2200/0636 , B01L2200/0652 , B01L2300/0627 , C12N13/00 , G01N1/44 , G01N15/1404 , G01N21/01 , G01N21/6486 , G01N33/4833 , G01N2015/1006 , G01N2015/1409 , G01N2015/1413
摘要： An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the microfluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.
摘要翻译： 识别物体的装置和方法包括：微流体芯片，其中布置有多个通道，所述微流体芯片包括：主流体通道，待识别的物体的样品流体混合物被引入到该主流体通道中; 多个护套流体通道，其中引入护套流体;护套流体，其沿着预定方向定向主流体通道中的物体，同时仍保持主流体通道中的层流; 询问装置，其检测并询问主流体通道中的定向物体; 以及对物体执行动作的聚焦能量装置。
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公开(公告)号：US20190187044A1
公开(公告)日：2019-06-20
申请号：US16279430
申请日：2019-02-19
发明人： David Appleyard , Jeff Betthauser , Marjorie Faust , John Larsen , Guocheng Shao , Zheng Xia , Yu Zhou
CPC分类号： G01N15/1484 , B01L3/502761 , B01L2200/0636 , B01L2200/0652 , B01L2300/0627 , C12N13/00 , G01N1/44 , G01N15/1404 , G01N21/01 , G01N21/6486 , G01N33/4833 , G01N2015/1006 , G01N2015/1409 , G01N2015/1413
摘要： A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
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公开(公告)号：US09588100B2
公开(公告)日：2017-03-07
申请号：US14308499
申请日：2014-06-18
发明人： David Appleyard , Jeff Betthauser , Marjorie Faust , John Larsen , Guocheng Shao , Zheng Xia , Yu Zhou
IPC分类号： G01N21/01 , G01N30/26 , G01N33/48 , G01N33/483 , G01N1/44 , G01N21/64 , G01N15/10 , G01N15/14
CPC分类号： G01N15/1484 , B01L3/502761 , B01L2200/0636 , B01L2200/0652 , B01L2300/0627 , C12N13/00 , G01N1/44 , G01N15/1404 , G01N21/01 , G01N21/6486 , G01N33/4833 , G01N2015/1006 , G01N2015/1409 , G01N2015/1413
摘要： An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the microfluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.
摘要翻译： 识别物体的装置和方法包括：微流体芯片，其中布置有多个通道，所述微流体芯片包括：主流体通道，待识别的物体的样品流体混合物被引入到该主流体通道中; 多个护套流体通道，其中引入护套流体;护套流体，其沿着预定方向定向主流体通道中的物体，同时仍保持主流体通道中的层流; 询问装置，其检测并询问主流体通道中的定向物体; 以及对物体执行动作的聚焦能量装置。
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公开(公告)号：US20180126376A1
公开(公告)日：2018-05-10
申请号：US15702452
申请日：2017-09-12
发明人： John Larsen , Yu Zhou , Zheng Xia
IPC分类号： B01L3/00 , C09D5/00 , C09D129/10
CPC分类号： B01L3/502707 , B01L3/0268 , B01L3/502715 , B01L2200/0636 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/089 , B01L2300/12 , B01L2300/163 , B01L2300/165 , C09D5/00 , C09D129/10
摘要： This disclosure concerns a method of providing a hydrophobic coating on a microfluidic chip that promotes the discrete flow of at least one liquid. It includes applying the hydrophobic coating onto an area of the microfluidic chip. The disclosure further includes a microfluidic chip that provides discrete flow of at least one liquid.
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