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公开(公告)号：US08633002B2
公开(公告)日：2014-01-21
申请号：US12854848
申请日：2010-08-11
申请人： Paul G. Roessler , Kevin Watts , Bo Liu
发明人： Paul G. Roessler , Kevin Watts , Bo Liu
CPC分类号： C12P7/04 , C12N9/0008 , C12N9/001
摘要： Genes and strains of recombinant microorganisms are provided that are engineered to produce fatty alcohols and fatty alcohol derivatives. The organisms can include one, two, three or more transgenes that direct the biosynthesis of one or more fatty alcohols or derivatives. Methods of producing fatty alcohols using transgenic microorganisms are also provided.
摘要翻译： 提供重组微生物的基因和菌株，其被工程化以产生脂肪醇和脂肪醇衍生物。 生物体可以包括一种，两种，三种或更多种转导子，其指导一种或多种脂肪醇或衍生物的生物合成。 还提供了使用转基因微生物生产脂肪醇的方法。
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公开(公告)号：US08975061B2
公开(公告)日：2015-03-10
申请号：US13536345
申请日：2012-06-28
申请人： Vincent A. Bielinski , Nicholas Bauman , Kate Lyons Boyd , Srividya Akella , Stanley Bower , Paul G. Roessler
发明人： Vincent A. Bielinski , Nicholas Bauman , Kate Lyons Boyd , Srividya Akella , Stanley Bower , Paul G. Roessler
摘要： The present invention relates to the regulation of a toxin and/or antitoxin genes in a genetically engineered microorganism, such as cyanobacterial or eukaryotic algal strains, in particular for preventing unintentional and/or uncontrolled spread of the microorganisms. The present invention also includes methods of controlling the growth and/or survival of the engineered microorganism
摘要翻译： 本发明涉及在遗传工程微生物如蓝藻或真核藻类菌株中调节毒素和/或抗毒素基因，特别是用于防止微生物的无意和/或不受控制的扩散。 本发明还包括控制工程微生物生长和/或存活的方法
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3.发明授权公开(公告)号：US07001772B2
公开(公告)日：2006-02-21
申请号：US10124807
申请日：2002-04-16
CPC分类号： C12N15/82 , C12N9/88 , C12N15/79 , C12P21/02 , C12Y202/01006
摘要： Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.
摘要翻译： 公开了乙酰乳酸合酶，乙酰乳酸合成酶调节区，α-微管蛋白启动子，破囊壶菌聚酮合成酶（PKS）系统的启动子和来自破囊壶菌菌体的脂肪酸去饱和酶启动子的核酸和氨基酸序列。 还公开了可用于转录破囊索杆菌微生物的重组载体，以及转变破囊壶菌微生物的方法。 本发明的重组核酸分子可用于在破囊壶菌属微生物中表达外源核酸，以及用于在裂囊酵素微生物中的基因的缺失，突变或失活。
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4.发明授权公开(公告)号：US08206984B2
公开(公告)日：2012-06-26
申请号：US11021712
申请日：2004-12-22
CPC分类号： C12N15/82 , C12N9/88 , C12N15/79 , C12P21/02 , C12Y202/01006
摘要： Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.
摘要翻译： 公开了乙酰乳酸合酶，乙酰乳酸合成酶调节区，α-微管蛋白启动子，来自破囊壶菌聚酮合成酶（PKS）系统的启动子和来自破囊壶菌属微生物的脂肪酸去饱和酶启动子的核酸和氨基酸序列。 还公开了可用于转录破囊索杆菌微生物的重组载体，以及转变破囊壶菌微生物的方法。 本发明的重组核酸分子可用于在破囊壶菌属微生物中表达外源核酸，以及用于在裂囊酵素微生物中的基因的缺失，突变或失活。
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5.发明授权Isolated gene encoding an enzyme with UDP-glucose pyrophosphorylase and phosphoglucomutase activities from Cyclotella cryptica 失效分离基因编码具有UDP-葡萄糖焦磷酸化酶的酶和来自Cyclotella cryptica的磷酸葡萄糖变位酶活性
公开(公告)号：US5928932A
公开(公告)日：1999-07-27
申请号：US627873
申请日：1996-04-03
申请人： Eric E. Jarvis , Paul G. Roessler
发明人： Eric E. Jarvis , Paul G. Roessler
CPC分类号： C12N9/1241 , C12N9/92
摘要： The present invention relates to a cloned gene which encodes an enzyme, the purified enzyme, and the applications and products resulting from the use of the gene and enzyme. The gene, isolated from Cyclotella cryptica, encodes a multifunctional enzyme that has both UDP-glucose pyrophosphorylase and phosphoglucomutase activities.
摘要翻译： 本发明涉及编码酶，纯化酶以及由使用该基因和酶产生的应用和产品的克隆基因。 从Cyclotella cryptica分离的基因编码具有UDP-葡萄糖焦磷酸化酶和磷酸果糖转移酶活性的多功能酶。
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6.发明授权
公开(公告)号：US5661017A
公开(公告)日：1997-08-26
申请号：US404732
申请日：1995-03-15
CPC分类号： C12N15/8241 , C12N9/93
摘要： Disclosed is a method to transform chlorophyll C-containing algae which includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae.
摘要翻译： 公开了一种转化含叶绿素C的藻类的方法，其包括将包含编码与藻类调节控制序列有效连接的显性选择标记的核酸分子的重组分子引入含叶绿素C的藻类中，使得标记为 由藻类生产。 在优选的实施方案中，藻类调节控制序列衍生自硅藻，优选来自Cyclotella cryptica。 还公开了具有一种或多种调节控制序列的嵌合分子，所述调节控制序列衍生自与可选择标记，RNA分子和/或蛋白质编码的核酸分子有效连接的一种或多种含有叶绿素C的藻类，其中所述核酸分子 通常不会发生一个或多个调控序列。 进一步具体公开的是分子pACCNPT10，pACCNPT4.8和pACCNPT5.1。 本发明的方法和材料提供了完成叶绿素C的藻类的稳定遗传转化的能力。
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公开(公告)号：US5559220A
公开(公告)日：1996-09-24
申请号：US418893
申请日：1995-04-07
申请人： Paul G. Roessler , John B. Ohlrogge
发明人： Paul G. Roessler , John B. Ohlrogge
CPC分类号： C12N9/93 , C12N15/8241
摘要： A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives thereof which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides.
摘要翻译： 编码来自光合生物的乙酰辅酶A羧化酶（ACCase）的DNA，其功能衍生物对某些除草剂具有抑制作用。 该基因可以放置在生物体中以增加其脂肪酸含量或使其对某些除草剂具有抗性。
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公开(公告)号：US20130023035A1
公开(公告)日：2013-01-24
申请号：US13536345
申请日：2012-06-28
申请人： Vincent A. Bielinski , Nicholas Bauman , Kate Lyons Boyd , Srividya Akella , Stanley Bower , Paul G. Roessler
发明人： Vincent A. Bielinski , Nicholas Bauman , Kate Lyons Boyd , Srividya Akella , Stanley Bower , Paul G. Roessler
摘要： The present invention relates to the regulation of a toxin and/or antitoxin genes in a genetically engineered microorganism, such as cyanobacterial or eukaryotic algal strains, in particular for preventing unintentional and/or uncontrolled spread of the microorganisms. The present invention also includes methods of controlling the growth and/or survival of the engineered microorganism
摘要翻译： 本发明涉及在遗传工程微生物如蓝藻或真核藻类菌株中调节毒素和/或抗毒素基因，特别是用于防止微生物的无意和/或不受控制的扩散。 本发明还包括控制工程微生物生长和/或存活的方法
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公开(公告)号：US20090158473A1
公开(公告)日：2009-06-18
申请号：US12327738
申请日：2008-12-03
申请人： Paul G. Roessler , Lada Rasochova , Vincent D. Lee
发明人： Paul G. Roessler , Lada Rasochova , Vincent D. Lee
CPC分类号： C12N15/8234 , C12N15/8247
摘要： A nucleotide sequence comprising a nucleotide sequence having at least about 90% homology to the sequence of SEQ ID NO:9 or SEQ ID NO:10. Nucleotide sequences interest operatively linked to nucleotide sequence having at least about 90% homology to the sequence of SEQ ID NO:9 or SEQ ID NO:10 are disclosed. Vectors, methods of regulating target expression, methods of providing a cells, plants, and seeds comprising the nucleotide sequence are also disclosed.
摘要翻译： 包含与SEQ ID NO：9或SEQ ID NO：10的序列具有至少约90％同源性的核苷酸序列的核苷酸序列。 公开了与SEQ ID NO：9或SEQ ID NO：10的序列具有至少约90％同源性的核苷酸序列可操作地连接的核苷酸序列。 还公开了载体，调节靶标表达的方法，提供包含该核苷酸序列的细胞，植物和种子的方法。
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