Product and process for transformation of Thraustochytriales microorganisms
    3.
    发明授权
    Product and process for transformation of Thraustochytriales microorganisms 有权
    破囊壶菌微生物转化的产物和方法

    公开(公告)号:US07001772B2

    公开(公告)日:2006-02-21

    申请号:US10124807

    申请日:2002-04-16

    IPC分类号: C07H21/04 C12N15/74 C12N1/12

    摘要: Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.

    摘要翻译: 公开了乙酰乳酸合酶,乙酰乳酸合成酶调节区,α-微管蛋白启动子,破囊壶菌聚酮合成酶(PKS)系统的启动子和来自破囊壶菌菌体的脂肪酸去饱和酶启动子的核酸和氨基酸序列。 还公开了可用于转录破囊索杆菌微生物的重组载体,以及转变破囊壶菌微生物的方法。 本发明的重组核酸分子可用于在破囊壶菌属微生物中表达外源核酸,以及用于在裂囊酵素微生物中的基因的缺失,突变或失活。

    Microbial production of fatty alcohols
    4.
    发明授权
    Microbial production of fatty alcohols 有权
    微生物生产脂肪醇

    公开(公告)号:US08633002B2

    公开(公告)日:2014-01-21

    申请号:US12854848

    申请日:2010-08-11

    IPC分类号: C12P7/02 C12P7/00

    CPC分类号: C12P7/04 C12N9/0008 C12N9/001

    摘要: Genes and strains of recombinant microorganisms are provided that are engineered to produce fatty alcohols and fatty alcohol derivatives. The organisms can include one, two, three or more transgenes that direct the biosynthesis of one or more fatty alcohols or derivatives. Methods of producing fatty alcohols using transgenic microorganisms are also provided.

    摘要翻译: 提供重组微生物的基因和菌株,其被工程化以产生脂肪醇和脂肪醇衍生物。 生物体可以包括一种,两种,三种或更多种转导子,其指导一种或多种脂肪醇或衍生物的生物合成。 还提供了使用转基因微生物生产脂肪醇的方法。

    Product and process for transformation of thraustochytriales microorganisms
    5.
    发明授权
    Product and process for transformation of thraustochytriales microorganisms 有权
    破囊壶菌微生物转化的产物和方法

    公开(公告)号:US08206984B2

    公开(公告)日:2012-06-26

    申请号:US11021712

    申请日:2004-12-22

    摘要: Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, α-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.

    摘要翻译: 公开了乙酰乳酸合酶,乙酰乳酸合成酶调节区,α-微管蛋白启动子,来自破囊壶菌聚酮合成酶(PKS)系统的启动子和来自破囊壶菌属微生物的脂肪酸去饱和酶启动子的核酸和氨基酸序列。 还公开了可用于转录破囊索杆菌微生物的重组载体,以及转变破囊壶菌微生物的方法。 本发明的重组核酸分子可用于在破囊壶菌属微生物中表达外源核酸,以及用于在裂囊酵素微生物中的基因的缺失,突变或失活。

    Method to transform algae, materials therefor, and products produced
thereby
    7.
    发明授权
    Method to transform algae, materials therefor, and products produced thereby 失效
    转化藻类,其材料的方法和由此生产的产品

    公开(公告)号:US5661017A

    公开(公告)日:1997-08-26

    申请号:US404732

    申请日:1995-03-15

    CPC分类号: C12N15/8241 C12N9/93

    摘要: Disclosed is a method to transform chlorophyll C-containing algae which includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae.

    摘要翻译: 公开了一种转化含叶绿素C的藻类的方法,其包括将包含编码与藻类调节控制序列有效连接的显性选择标记的核酸分子的重组分子引入含叶绿素C的藻类中,使得标记为 由藻类生产。 在优选的实施方案中,藻类调节控制序列衍生自硅藻,优选来自Cyclotella cryptica。 还公开了具有一种或多种调节控制序列的嵌合分子,所述调节控制序列衍生自与可选择标记,RNA分子和/或蛋白质编码的核酸分子有效连接的一种或多种含有叶绿素C的藻类,其中所述核酸分子 通常不会发生一个或多个调控序列。 进一步具体公开的是分子pACCNPT10,pACCNPT4.8和pACCNPT5.1。 本发明的方法和材料提供了完成叶绿素C的藻类的稳定遗传转化的能力。

    SEED-PREFERRED GENE PROMOTERS FROM THE CASTOR PLANT
    9.
    发明申请
    SEED-PREFERRED GENE PROMOTERS FROM THE CASTOR PLANT 审中-公开
    种子植物种子优先基因促进剂

    公开(公告)号:US20090158473A1

    公开(公告)日:2009-06-18

    申请号:US12327738

    申请日:2008-12-03

    CPC分类号: C12N15/8234 C12N15/8247

    摘要: A nucleotide sequence comprising a nucleotide sequence having at least about 90% homology to the sequence of SEQ ID NO:9 or SEQ ID NO:10. Nucleotide sequences interest operatively linked to nucleotide sequence having at least about 90% homology to the sequence of SEQ ID NO:9 or SEQ ID NO:10 are disclosed. Vectors, methods of regulating target expression, methods of providing a cells, plants, and seeds comprising the nucleotide sequence are also disclosed.

    摘要翻译: 包含与SEQ ID NO:9或SEQ ID NO:10的序列具有至少约90%同源性的核苷酸序列的核苷酸序列。 公开了与SEQ ID NO:9或SEQ ID NO:10的序列具有至少约90%同源性的核苷酸序列可操作地连接的核苷酸序列。 还公开了载体,调节靶标表达的方法,提供包含该核苷酸序列的细胞,植物和种子的方法。