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13 条记录 (耗时 0.036 秒)

    Target nucleic acid amplification/detection systems and methods for the use thereof
    2.
    发明授权
    Target nucleic acid amplification/detection systems and methods for the use thereof 失效
    靶核酸扩增/检测系统及其使用方法

    公开(公告)号：US5631129A

    公开(公告)日：1997-05-20

    申请号：US441784

    申请日：1995-05-16

    申请人： Barbara C. Chu ,  Gerald F. Joyce ,  Leslie E. Orgel

    发明人： Barbara C. Chu ,  Gerald F. Joyce ,  Leslie E. Orgel

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6867 ,  Y10S435/81

    摘要： This invention relates to the use of functional reporter molecules in the detection and measurement of RNA sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of nucleotide sequences, one having a probe sequence linked to a sequence capable of initiating replication by an RNA-dependent RNA polymerase. The other is capable of hybridizing to a strand separated from the extension product of the first nucleotide sequence after hybridization to a specific target sequence. The extension product of the second hybridized nucleotide sequence serves as a template source for autocatalytic replication by the RNA-dependent RNA polymerase. The replication products are detected as a means for detection of nucleic acid sequences.

    摘要翻译： 本发明涉及功能性报道分子在检测和测量样品中的RNA序列中的用途，作为例如病原性疾病存在或潜在的决定。 本发明基于核苷酸序列的利用，一个具有与能够通过RNA依赖性RNA聚合酶启动复制的序列连接的探针序列。 另一种能够在与特定靶序列杂交后与从第一核苷酸序列的延伸产物分离的链杂交。 第二杂交核苷酸序列的延伸产物作为RNA依赖性RNA聚合酶自动复制的模板来源。 检测复制产物作为检测核酸序列的手段。

    Replicative RNA-based amplification/detection systems
    3.
    发明授权
    Replicative RNA-based amplification/detection systems 失效
    基于RNA的复制/检测系统

    公开(公告)号：US5532126A

    公开(公告)日：1996-07-02

    申请号：US127986

    申请日：1993-09-27

    申请人： Barbara C. Chu ,  Gerald F. Joyce ,  Leslie E. Orgel

    发明人： Barbara C. Chu ,  Gerald F. Joyce ,  Leslie E. Orgel

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C07H21/00 ,  C12Q1/6867 ,  C12Q1/701

    摘要： This invention relates to the use of functional reporter molecules in the detection and measurement of nucleic acid sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of a transcription step between the production of an appropriate reporter molecule and replication based amplification in order to increase the number of detectable species as an indirect reference to target nucleic acid sequence.

    摘要翻译： 本发明涉及功能报告分子在检测和测量样品中的核酸序列中的用途，作为例如致病性疾病存在或潜在的确定。 本发明基于在适当的报道分子的产生和基于复制的扩增之间的转录步骤的利用，以便增加作为靶核酸序列的间接参考的可检测物种的数量。

    Coupled isothermal polynucleotide amplification and translation system
    4.
    发明授权
    Coupled isothermal polynucleotide amplification and translation system 失效
    耦合等温多核苷酸扩增和翻译系统

    公开(公告)号：US5807717A

    公开(公告)日：1998-09-15

    申请号：US231587

    申请日：1994-04-20

    申请人： Gerald F. Joyce

    发明人： Gerald F. Joyce

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6865 ,  C12Q1/686

    摘要： A cell-free system for polynucleotide amplification and translation is disclosed. Also disclosed are methods for using the system and a composition which allows the various components of the system to function under a common set of reaction conditions.

    摘要翻译： 公开了一种用于多核苷酸扩增和翻译的无细胞系统。 还公开了使用该系统和允许系统的各种组分在一组共同的反应条件下起作用的组合物的方法。

    Catalytic RNA molecules
    8.
    发明授权
    Catalytic RNA molecules 失效
    催化RNA分子

    公开(公告)号：US6063566A

    公开(公告)日：2000-05-16

    申请号：US682423

    申请日：1996-07-17

    申请人： Gerald F. Joyce

    发明人： Gerald F. Joyce

    IPC分类号： C12N15/09 ,  A61K31/7105 ,  A61K38/00 ,  C07H21/02 ,  C07K14/00 ,  C12N9/00 ,  C12N15/113 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N15/113 ,  A61K38/00 ,  C12N2310/111 ,  C12N2310/124 ,  C12N2310/1241

    摘要： The present invention discloses nucleic acid enzymes capable of cleaving nucleic acid molecules, including single-stranded DNA, in a site-specific manner under physiologic conditions, as well as compositions including same. The present invention also discloses methods of making and using the disclosed enzymes and compositions.The present invention further discloses nucleic acid enzymes or catalytic (enzymatic) RNA molecules that are capable of cleaving a variety of bonds, including phosphodiester bonds and amide bonds, in a variety of substrates. Thus, various disclosed enzymatic RNA molecules are capable of functioning as nucleases, amidases, and/or peptidases. The present invention also relates to compositions containing the disclosed catalytic RNA molecules and to methods of making, selecting, and using such enzymes and compositions.

    摘要翻译： 本发明公开了能够在生理条件下以位点特异性方式切割核酸分子（包括单链DNA）的核酸酶，以及包含其的组合物。 本发明还公开了制备和使用所公开的酶和组合物的方法。 本发明还公开了能够在各种底物中切割多种键（包括磷酸二酯键和酰胺键）的核酸酶或催化（酶）RNA分子。 因此，各种公开的酶RNA分子能够用作核酸酶，酰胺酶和/或肽酶。 本发明还涉及包含所公开的催化性RNA分子的组合物以及制备，选择和使用这些酶和组合物的方法。

    Enzymatic nucleic acid molecules
    10.
    发明授权
    Enzymatic nucleic acid molecules 有权
    酶的核酸分子

    公开(公告)号：US07807817B2

    公开(公告)日：2010-10-05

    申请号：US11605177

    申请日：2006-11-28

    申请人： Gerald F. Joyce ,  Ronald R. Breaker

    发明人： Gerald F. Joyce ,  Ronald R. Breaker

    IPC分类号： C07H21/04 ,  A61K31/70

    CPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q2521/337

    摘要： The present invention discloses nucleic acid enzymes and deoxyribonucleic acid enzymes capable of cleaving nucleic acid sequences or molecules, particularly RNA, in a site-specific manner, as well as compositions including same. Methods of making and using the disclosed enzymes and compositions are also disclosed.

    摘要翻译： 本发明公开了能够以位点特异性方式切割核酸序列或分子，特别是RNA的核酸酶和脱氧核糖核酸酶，以及包含其的组合物。 还公开了制备和使用所公开的酶和组合物的方法。