公开(公告)号：US20070178497A1

公开(公告)日：2007-08-02

申请号：US11615046

申请日：2006-12-22

申请人： Peter Gill ,  Javaid Hussain ,  Adam Long ,  Gillian Tully

发明人： Peter Gill ,  Javaid Hussain ,  Adam Long ,  Gillian Tully

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： C12Q1/6881 ,  C12Q1/6858 ,  C12Q2600/156 ,  G16B30/00 ,  G16B40/00 ,  C12Q2537/143 ,  C12Q2535/125

摘要： The invention provides a method for obtaining additional information about DNA mixtures arising from a variety of sources and/or a variety of concentrations. In particular, the invention provides a method for indicating the likelihood that a DNA mixture arose from sources of a defined type where: the DNA mixture is formed by DNA samples from more than one source, the method involving the determination of the identity of the alleles present at a locus for the DNA in the mixture; determining a first probability function for the situation where the DNA mixture is formed from samples arising from the given person and from a first other person; determining a second probability function for the situation where the DNA mixture is formed from samples arising from a second other person and a first other person; using the first probability function as numerator and the second probability function as denominator in determining a likelihood ratio for the mixture having arisen from the defined type of sources considered in the first probability function; determining such likelihood ratios for a plurality of loci; and combining the likelihood ratios to give a combined likelihood ratio for the mixture having arisen from the defined type of sources considered in the first probability function.

摘要翻译： 本发明提供了一种获得关于由各种来源和/或多种浓度产生的DNA混合物的附加信息的方法。 特别地，本发明提供了一种用于指示DNA混合物从定义类型的来源产生的可能性的方法，其中：DNA混合物由来自多于一个来源的DNA样品形成，所述方法涉及确定等位基因的身份 存在于混合物中DNA的位点; 确定由给定人和第一另一个人产生的样品形成DNA混合物的情况的第一概率函数; 确定由第二另一个人和第一另一个人产生的样品形成DNA混合物的情况的第二概率函数; 使用第一概率函数作为分子，并且将第二概率函数作为分母来确定从在第一概率函数中考虑的限定类型的源产生的混合物的似然比; 确定多个基因座的似然比; 并且将似然比组合以给出从在第一概率函数中考虑的所定义类型的源产生的混合物的组合似然比。

公开(公告)号：US20050064500A1

公开(公告)日：2005-03-24

申请号：US10977698

申请日：2004-10-29

申请人： Peter Gill ,  Jonathon Whitaker ,  John Buckleton

发明人： Peter Gill ,  Jonathon Whitaker ,  John Buckleton

IPC分类号： C12Q1/68 ,  G06F19/18 ,  G06F19/22

CPC分类号： G16B30/00 ,  G16B20/00

摘要： The invention provides an improved method for obtaining information about DNA analysis of samples of uncertain origin by establishing the likelihood that they arose in certain manners compared with other possible manners. In this way all of the analysis information is taken into account and likelihood ratios are provided to express the results. The invention is particularly useful in analysing small DNA samples or DNA samples where the contribution from one or more sources is small.

摘要翻译： 本发明提供了一种改进的方法，用于通过确定与其他可能方式相比以某种方式产生的可能性来获得关于不确定来源的样品的DNA分析的信息。 以这种方式考虑所有的分析信息，并提供似然比来表达结果。 本发明特别可用于分析来自一个或多个来源的贡献较小的小DNA样品或DNA样品。

公开(公告)号：US20050014183A1

公开(公告)日：2005-01-20

申请号：US10892854

申请日：2004-07-15

申请人： Peter Gill ,  Javaid Hussain ,  Adam Long

发明人： Peter Gill ,  Javaid Hussain ,  Adam Long

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6876 ,  C12Q2600/156 ,  C12Q2600/16

摘要： Primer mixtures and methods of investigating single nucleotide polymorphisms in samples of DNA are provided having particular primer sequences. The primer mixtures and methods are aimed at achieving optimised performance in multiplexes.

摘要翻译： 提供引物混合物和研究DNA样品中单核苷酸多态性的方法，其具有特定的引物序列。 引物混合物和方法旨在实现多路复用中的优化性能。

公开(公告)号：US08032307B2

公开(公告)日：2011-10-04

申请号：US11937825

申请日：2007-11-09

申请人： Peter Gill ,  Lindsey Dixon

发明人： Peter Gill ,  Lindsey Dixon

IPC分类号： G01N33/48

CPC分类号： C12Q1/6827

摘要： A method for establishing the genotype of the locus is provided in which a series of calibration samples are analysed, the results being one of three indication types, a window being defined relative to the indication for each of the indication types, unknown samples being similarly analysed with the window that they fall within being taken to determine the indication type for the unknown sample and hence the genotype of the relevant locus. The technique provides a robust, reliable and accurate method of genotyping which is suited to automation.

摘要翻译： 提供了一种用于建立基因座基因型的方法，其中分析了一系列校准样本，结果是三种指示类型之一，相对于每种指示类型的指示定义了窗口，类似地分析了未知样本 随着它们落入的窗口被采取以确定未知样品的指示类型，并因此确定相关基因座的基因型。 该技术提供了一种强大，可靠和准确的基因分型方法，适用于自动化。

公开(公告)号：US07970551B2

公开(公告)日：2011-06-28

申请号：US12686174

申请日：2010-01-12

申请人： Peter Gill ,  Amanda Kirkham

发明人： Peter Gill ,  Amanda Kirkham

IPC分类号： G01N33/48

CPC分类号： C12Q1/6809

摘要： The invention provides methods for providing information on the probability of DNA samples being contaminated over days, weeks and months in relation to collection, processing and analysis of DNA samples. Methods are also provided for analyzing the likelihood of a result arising due to contamination and/or determining the analysis protocol to be applied to a DNA sample and/or methods of operating databases, particularly containing DNA profiles.Improvements in accounting for sporadic and undetected contamination and improvements in the operation of DNA sampling of analysis are provided by the invention.

摘要翻译： 本发明提供了关于DNA样品在DNA样品的收集，处理和分析方面在几天，几周和几个月内被污染的可能性的信息的方法。 还提供了用于分析由于污染引起的结果的可能性和/或确定要应用于DNA样品的分析方案和/或操作数据库的方法，特别是含有DNA谱的方法。 通过本发明提供了散发性和未检测到的污染物的改进，以及分析的DNA取样操作的改进。

公开(公告)号：US20090117579A1

公开(公告)日：2009-05-07

申请号：US12346052

申请日：2008-12-30

申请人： Adam Long ,  Peter Gill ,  Tim Cox

发明人： Adam Long ,  Peter Gill ,  Tim Cox

IPC分类号： C12Q1/68 ,  C07H1/06

CPC分类号： B01J20/10 ,  B01L3/502707 ,  B01L2200/0631 ,  B01L2300/0883 ,  C12N15/1006 ,  G01N1/40 ,  G01N1/405 ,  G01N30/00 ,  G01N2030/009 ,  G01N30/6095

摘要： A variety of methods are provided which use a silicon or silicon dioxide channel to extract DNA from a sample and then release it at a later point. The extraction channels are simple to manufacture and reliable in use. Prior art problems with entrainment of gas, liquid and solid material within channels are addressed. The techniques provide a convenient way of controlling the amount or concentration of DNA in the eluant.

摘要翻译： 提供了各种方法，其使用硅或二氧化硅通道从样品中提取DNA，然后在稍后将其释放。 提取通道制造简单，使用可靠。 解决了在通道内夹带气体，液体和固体材料的现有技术问题。 这些技术提供了一种方便的方式来控制洗脱液中DNA的量或浓度。

公开(公告)号：US5012225A

公开(公告)日：1991-04-30

申请号：US451294

申请日：1989-12-15

申请人： Peter Gill

发明人： Peter Gill

IPC分类号： G08B13/24

CPC分类号： G08B13/242 ,  G08B13/2437

摘要： A field-sensitive tag or label incorporates a layer for mechanically deactivating the tag resulting from subsequent use of the article to which the tag has been applied. To this end, a layered tag is provided which, in addition to the layers which are traditionally provided to achieve the function of a resonant circuit (e.g., substrate and etched outer layers), further incorporates a circuit-deactivating layer which preferably takes the form of an abrasive substance disposed in a suitable binder. The resulting layer can then operate to mechanically deactivate the tag by damaging at least a portion of the resonant circuit which comprises the tag due to movement of the article in subsequent use. The field-sensitive tag of the present invention is advantageously applied to footwear in regions where flexure of the tag can be anticipated, preferably in the sole, making the article well suited to placement in a self-service retail setting while maintaining an adequate degree of security.

摘要翻译： 场敏感标签或标签包含用于机械地去激活标签的层，该层由随后使用已经应用标签的制品而产生。 为此，提供了一种分层标签，除了传统上提供以实现谐振电路（例如，衬底和蚀刻的外层）的功能的层之外，还进一步包括电路失活层，其优选地采用形式 设置在合适的粘合剂中的研磨物质。 所得到的层然后可以通过损坏包括标签的谐振电路的至少一部分而由于随后的使用中的物品的移动而使标签机械地失活。 本发明的场敏感标签有利地应用于可以预期标签弯曲的区域中的鞋类，优选地在鞋底中，使得该制品非常适合放置在自助零售设置中，同时保持适当程度的 安全。

公开(公告)号：US07668660B2

公开(公告)日：2010-02-23

申请号：US10564682

申请日：2004-07-09

申请人： Peter Gill ,  Amanda Kirkham

发明人： Peter Gill ,  Amanda Kirkham

IPC分类号： G01N33/48

CPC分类号： C12Q1/6809

摘要： The invention provides methods for providing information on the probability of DNA samples being contaminated over days, weeks and months in relation to collection, processing and analysis of DNA samples. Methods are also provided for analysing the likelihood of a result arising due to contamination and/or determining the analysis protocol to be applied to a DNA sample and/or methods of operating databases, particularly containing DNA profiles.Improvements in accounting for sporadic and undetected contamination and improvements in the operation of DNA sampling of analysis are provided by the invention.

摘要翻译： 本发明提供了关于DNA样品在DNA样品的收集，处理和分析方面在几天，几周和几个月内被污染的可能性的信息的方法。 还提供了用于分析由于污染引起的结果的可能性和/或确定要应用于DNA样品的分析方案和/或操作数据库的方法，特别是含有DNA谱的方法。 通过本发明提供了散发性和未检测到的污染物的改进，以及分析的DNA取样操作的改进。

公开(公告)号：US20090234621A1

公开(公告)日：2009-09-17

申请号：US11720721

申请日：2005-12-05

申请人： Peter Gill ,  James Curran

发明人： Peter Gill ,  James Curran

IPC分类号： G06F17/10 ,  G06G7/48 ,  G06F19/00 ,  G01N33/48

CPC分类号： C12Q1/6851 ,  G16B40/00 ,  G16B45/00 ,  C12Q2537/165

摘要： A method of optimizing one or more parameters in a process for considering a DNA containing sample using a method of modeling and a method of modeling itself are provided. The method of modeling the process for considering a DNA containing sample uses a graphical model. The model seeks to provide one or more optimized parameters for the consideration process. The methods aim to consider the whole process, for instance, the number of cells required for the process and/or the extraction efficiency and/or the sub-sample volume relative to the sample volume and/or the amplification efficiency and/or the optimum number of amplification cycles and/or the effect of degradation on the amount of amplifiable DNA in the sample.

摘要翻译： 提供了一种在使用建模方法考虑含DNA样品的过程中优化一个或多个参数的方法和自身建模方法。 用于考虑含DNA样品的过程进行建模的方法使用图形模型。 该模型试图为考虑过程提供一个或多个优化参数。 该方法旨在考虑整个过程，例如，相对于样品体积和/或放大效率和/或最佳值，该方法所需的细胞数量和/或提取效率和/或亚样品体积 扩增循环数和/或降解对样品中可扩增DNA量的影响。

公开(公告)号：US20080161196A1

公开(公告)日：2008-07-03

申请号：US11937825

申请日：2007-11-09

申请人： Peter Gill ,  Lindsey Dixon

发明人： Peter Gill ,  Lindsey Dixon

IPC分类号： C40B30/00 ,  C12Q1/68

CPC分类号： C12Q1/6827

摘要： A method for establishing the genotype of the locus is provided in which a series of calibration samples are analysed, the results being one of three indication types, a window being defined relative to the indication for each of the indication types, unknown samples being similarly analysed with the window that they fall within being taken to determine the indication type for the unknown sample and hence the genotype of the relevant locus.The technique provides a robust, reliable and accurate method of genotyping which is suited to automation.

摘要翻译： 提供了一种用于建立基因座基因型的方法，其中分析了一系列校准样本，结果是三种指示类型之一，相对于每种指示类型的指示定义了窗口，类似地分析了未知样本 随着它们落入的窗口被采取以确定未知样品的指示类型，并因此确定相关基因座的基因型。 该技术提供了一种强大，可靠和准确的基因分型方法，适用于自动化。