公开(公告)号：US09164037B2

公开(公告)日：2015-10-20

申请号：US11698338

申请日：2007-01-26

申请人： Michael Bassler ,  Peter Kiesel ,  Oliver Schmidt

发明人： Michael Bassler ,  Peter Kiesel ,  Oliver Schmidt

IPC分类号： G01N33/48 ,  G01N21/64 ,  G06F19/00 ,  G06F19/12 ,  G01N31/00 ,  G06G7/58 ,  G01N15/10 ,  G01N15/14

CPC分类号： G01N21/6408 ,  G01N21/6458 ,  G01N2015/1075 ,  G01N2015/1486 ,  G06F19/12 ,  G06F2217/16 ,  G16H50/20

摘要： A method is provided for extracting the position of a particle, e.g., a moving or stationary particle that is excited or is emitting light. The method includes, among other steps, detecting and recording a signal based on, for example, the movement of the particle, a correlation step to eliminate noise and to create a transformed signal, a matching or fitting step to match the transformed signal to a fit function and an extracting or determining step to determine the position of the particle from the fit function. In one form, at least two particle positions are detected so that the distance between the subject particles can be determined.

摘要翻译： 提供了用于提取颗粒的位置的方法，例如被激发或正在发光的移动或静止的颗粒。 除了其他步骤之外，该方法包括基于例如粒子的移动来检测和记录信号，消除噪声并产生变换信号的相关步骤，匹配或拟合步骤，以将变换后的信号与 拟合函数和提取或确定步骤以从拟合函数确定粒子的位置。 在一种形式中，检测至少两个颗粒位置，使得可以确定主体颗粒之间的距离。

公开(公告)号：US08120782B2

公开(公告)日：2012-02-21

申请号：US12603627

申请日：2009-10-22

申请人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler ,  Uma Srinivasan

发明人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler ,  Uma Srinivasan

IPC分类号： G01B9/02

CPC分类号： G01J3/26 ,  G01N21/45 ,  G01N2021/451 ,  G02B26/001

摘要： A tunable optical cavity can be tuned by relative movement between two reflection surfaces, such as by deforming elastomer spacers connected between mirrors or other light-reflective components that include the reflection surfaces. The optical cavity structure includes an analyte region in its light-transmissive region, and presence of analyte in the analyte region affects output light when the optical cavity is tuned to a set of positions. Electrodes that cause deformation of the spacers can also be used to capacitively sense the distance between them. Control circuitry that provides tuning signals can cause continuous movement across a range of positions, allowing continuous photosensing of analyte-affected output light by a detector.

摘要翻译： 可调光学腔可以通过两个反射表面之间的相对运动进行调节，例如通过变形连接在反射镜之间的弹性体隔离物或包括反射表面的其它光反射部件。 光腔结构包括其透光区域中的分析物区域，并且当光学腔被调谐到一组位置时，分析物区域中的分析物的存在影响输出光。 导致间隔物变形的电极也可用于电容地感测它们之间的距离。 提供调谐信号的控制电路可以在一定范围的位置上引起连续的运动，从而允许由检测器对被分析物影响的输出光进行持续的光敏。

公开(公告)号：US07936463B2

公开(公告)日：2011-05-03

申请号：US11702325

申请日：2007-02-05

申请人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler

发明人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler

IPC分类号： G01B9/02

CPC分类号： G01N21/0303 ,  G01N21/05 ,  G01N21/253 ,  G01N2021/0346

摘要： A device can include both a photosensing component and an optical cavity structure, with the optical cavity structure including a part that can operate as an optical cavity in response to input light, providing laterally varying output light. For example, the optical cavity can be a graded linearly varying filter (LVF) or other inhomogeneous optical cavity, and the photosensing component can have a photosensitive surface that receives its output light without it passing through another optical component, thus avoiding loss of information. The optical cavity part can include a region that can contain analyte. Presence of the analyte affects the optical cavity part's output light, and the photosensing component can respond to the output light, providing sensing results indicating the analyte's optical characteristics.

摘要翻译： 设备可以包括光敏元件和光学腔结构，其中光学腔结构包括响应于输入光可以作为光腔工作的部件，提供横向变化的输出光。 例如，光学腔可以是渐变的线性变化的滤光器（LVF）或其他不均匀的光学腔，并且光敏元件可以具有接收其输出光而不通过另一个光学部件的感光表面，从而避免信息的丢失。 光腔部分可以包括可以含有分析物的区域。 分析物的存在影响光腔部分的输出光，并且光敏元件可以对输出光进行响应，提供指示分析物的光学特性的感测结果。

公开(公告)号：US07817281B2

公开(公告)日：2010-10-19

申请号：US11702320

申请日：2007-02-05

申请人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler ,  Uma Srinivasan

发明人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler ,  Uma Srinivasan

IPC分类号： G01B9/02

CPC分类号： H01S3/08081 ,  G01J3/26 ,  H01S3/105

摘要： An inhomogeneous optical cavity is tuned by changing its shape, such as by changing reflection surface positions to change tilt angle, thickness, or both. Deformable components such as elastomer spacers can be connected so that, when deformed, they change relative positions of structures with light-reflective components such as mirrors, changing cavity shape. Electrodes can cause deformation, such as electrostatically, electromagnetically, or piezoelectrically, and can also be used to measure thicknesses of the cavity. The cavity can be tuned, for example, across a continuous spectrum, to a specific wavelength band, to a shape that increases or decreases the number of modes it has, to a series of transmission ranges each suitable for a respective light source, with a modulation that allows lock-in with photosensing for greater sensitivity, and so forth. The optical cavity can be a linear variable filter fabricated on the photosensitive surface of a photosensing component such as a photosensor array or a position-sensitive detector.

摘要翻译： 通过改变其形状来调整不均匀的光学腔，例如通过改变反射面位置来改变倾斜角，厚度或两者。 变形部件如弹性体间隔件可以连接，使得当变形时，它们改变结构与诸如反射镜的光反射部件的相对位置，改变腔体形状。 电极可能导致变形，例如静电，电磁或压电，也可用于测量腔的厚度。 空腔可以例如通过连续光谱被调谐到特定波长带，以使其具有的模式数量增加或减少的形状调整到各自适用于相应光源的一系列传输范围，其中 允许通过光敏锁定来实现更高灵敏度的调制，等等。 光腔可以是制造在诸如光电传感器阵列或位置敏感检测器的感光部件的感光表面上的线性可变滤波器。

公开(公告)号：US07554673B2

公开(公告)日：2009-06-30

申请号：US11702470

申请日：2007-02-05

申请人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler

发明人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler

IPC分类号： G01B9/02

CPC分类号： G01N21/031 ,  B01L3/5085 ,  B01L2200/10 ,  B01L2300/168 ,  G01J3/26 ,  G01N2021/391

摘要： While two or more analytes within an optical cavity move relative to an array of photosensing elements, the cavity provides output light that has a position/time varying intensity function that depends on optical characteristics of the analytes and on the relative movement. The output light is photosensed to obtain sensing results that depend on the position/time varying intensity function. The sensing results are used to obtain information about at least one of the analytes. The relative movement can, for example, be caused by moving analytes within channels within the cavity, such as by causing flow of a medium that carries the analytes through the channels. Or the analytes can be in wells of a biochip, with the cavity defined by reflective slides on opposite surfaces of the biochip, and the slides and biochip can be caused to move together relative to the array.

摘要翻译： 虽然光学腔内的两个或更多个分析物相对于光敏元件阵列移动，但腔提供具有取决于分析物的光学特性和相对运动的位置/时间变化强度函数的输出光。 输出光被光照以获得取决于位置/时变强度函数的感测结果。 感测结果用于获得关于至少一种分析物的信息。 例如，相对运动可以通过在腔内的通道内移动分析物，例如通过使携带分析物的介质流过通道而引起。 或者分析物可以在生物芯片的孔中，其中空腔由生物芯片的相对表面上的反射片限定，并且可以使载片和生物芯片相对于阵列一起移动。

公开(公告)号：US20080186500A1

公开(公告)日：2008-08-07

申请号：US11702249

申请日：2007-02-05

申请人： Oliver Schmidt ,  Peter Kiesel ,  Michael Bassler

发明人： Oliver Schmidt ,  Peter Kiesel ,  Michael Bassler

IPC分类号： G01B9/02

CPC分类号： G01N21/39 ,  G01N21/0303 ,  G01N21/253

摘要： Output light from an optical cavity includes, for each of a set of modes, an intensity function, and a mode's intensity function includes information, such as about an optical characteristic of an analyte or of a region. For example, the intensity function can include a peak, and its central energy, maximum intensity, contrast, or intermediate intensity width (e.g. FWHM) can indicate the optical characteristic. The output light can be photosensed, providing electrical signals that depend on the optical characteristic. Information about the analyte or region can then be obtained using the electrical signals. For example, the information can be about both refractive index and absorption of an analyte. Cavity-only absorption values, independent, for example, of absorption outside the cavity and of inhomogeneous illumination, can be obtained based on contrast or intermediate intensity width. For detection of glucose in bodily fluid, derivatives of absorption can be obtained.

摘要翻译： 来自光学腔的输出光对于一组模式中的每一个包括强度函数，并且模式的强度函数包括诸如关于分析物或区域的光学特性的信息。 例如，强度函数可以包括峰值，并且其中心能量，最大强度，对比度或中间强度宽度（例如，FWHM）可以指示光学特性。 输出光可以被光照，提供取决于光学特性的电信号。 然后可以使用电信号获得关于分析物或区域的信息。 例如，该信息可以关于分析物的折射率和吸收。 基于对比度或中等强度宽度，可以获得独立于例如空腔外的吸收和不均匀照射的仅腔吸收值。 为了检测体液中的葡萄糖，可以得到吸收的衍生物。

公开(公告)号：US20080186494A1

公开(公告)日：2008-08-07

申请号：US11702325

申请日：2007-02-05

申请人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler

发明人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler

IPC分类号： G01N21/00

CPC分类号： G01N21/0303 ,  G01N21/05 ,  G01N21/253 ,  G01N2021/0346

摘要： A device can include both a photosensing component and an optical cavity structure, with the optical cavity structure including a part that can operate as an optical cavity in response to input light, providing laterally varying output light. For example, the optical cavity can be a graded linearly varying filter (LVF) or other inhomogeneous optical cavity, and the photosensing component can have a photosensitive surface that receives its output light without it passing through another optical component, thus avoiding loss of information. The optical cavity part can include a region that can contain analyte. Presence of the analyte affects the optical cavity part's output light, and the photosensing component can respond to the output light, providing sensing results indicating the analyte's optical characteristics.

摘要翻译： 设备可以包括光敏元件和光学腔结构，其中光学腔结构包括响应于输入光可以作为光腔工作的部件，提供横向变化的输出光。 例如，光学腔可以是渐变的线性变化的滤光器（LVF）或其他不均匀的光学腔，并且光敏元件可以具有接收其输出光而不通过另一个光学部件的感光表面，从而避免信息的丢失。 光腔部分可以包括可以含有分析物的区域。 分析物的存在影响光腔部分的输出光，并且光敏元件可以对输出光进行响应，提供指示分析物的光学特性的感测结果。

公开(公告)号：US20100040981A1

公开(公告)日：2010-02-18

申请号：US12603627

申请日：2009-10-22

申请人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler ,  Uma Srinivasan

发明人： Peter Kiesel ,  Oliver Schmidt ,  Michael Bassler ,  Uma Srinivasan

IPC分类号： G03F7/20 ,  B29D11/00 ,  B05D5/06

CPC分类号： G01J3/26 ,  G01N21/45 ,  G01N2021/451 ,  G02B26/001

摘要： A tunable optical cavity can be tuned by relative movement between two reflection surfaces, such as by deforming elastomer spacers connected between mirrors or other light-reflective components that include the reflection surfaces. The optical cavity structure includes an analyte region in its light-transmissive region, and presence of analyte in the analyte region affects output light when the optical cavity is tuned to a set of positions. Electrodes that cause deformation of the spacers can also be used to capacitively sense the distance between them. Control circuitry that provides tuning signals can cause continuous movement across a range of positions, allowing continuous photosensing of analyte-affected output light by a detector.

摘要翻译： 可调光学腔可以通过两个反射表面之间的相对运动进行调节，例如通过变形连接在反射镜之间的弹性体隔离物或包括反射表面的其它光反射部件。 光腔结构包括其透光区域中的分析物区域，并且当光学腔被调谐到一组位置时，分析物区域中的分析物的存在影响输出光。 导致间隔物变形的电极也可用于电容地感测它们之间的距离。 提供调谐信号的控制电路可以在一定范围的位置上引起连续的运动，从而允许由检测器对被分析物影响的输出光进行持续的光敏。

公开(公告)号：US20090016690A1

公开(公告)日：2009-01-15

申请号：US11777661

申请日：2007-07-13

申请人： Oliver Schmidt ,  Michael Bassler ,  Peter Kiesel

发明人： Oliver Schmidt ,  Michael Bassler ,  Peter Kiesel

IPC分类号： G02B6/10

CPC分类号： G02B6/10 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0256 ,  G01J3/0259 ,  G01N21/0303 ,  G01N21/645 ,  G01N21/76 ,  G01N2021/0346 ,  G02B6/032

摘要： Complementary surface fabrication processes such as molding, casting, embossing, and so forth, are used to produce articles, structures, or components structured to operate as sandwich waveguides. Resulting complementary surface artifacts include, for example, optical quality surfaces on wall parts, other exposed artifacts that occur where a complementary solid surface contacts non-solid material during fabrication, and sub-surface artifacts such as integrally formed connections between wall parts and base parts. A body whose surface includes a waveguide's inward surfaces, outward surfaces, and light interface surfaces to receive incident light can be formed in a single step, leaving a partially bounded fluidic region that can then be covered to provide a channel that is bounded along a length yet open at its ends; other fluidic, electrical, and optical components can also be attached.

摘要翻译： 使用诸如模制，铸造，压花等的互补表面制造工艺来生产构造成作为夹层波导操作的制品，结构或部件。 所产生的互补表面伪影包括例如壁部件上的光学质量表面，在制造期间互补的固体表面与非固体材料接触时发生的其他暴露的假象以及诸如壁部件和基部之间的一体形成的连接的子表面伪影 。 可以在单个步骤中形成表面包括波导的内表面，外表面和光界面的表面的主体，留下部分界定的流体区域，然后可以将其覆盖以提供沿着长度限定的通道 尚未开放; 也可以连接其他流体，电和光学部件。

公开(公告)号：US20090016672A1

公开(公告)日：2009-01-15

申请号：US11777712

申请日：2007-07-13

申请人： Oliver Schmidt ,  Michael Bassler ,  Peter Kiesel

发明人： Oliver Schmidt ,  Michael Bassler ,  Peter Kiesel

IPC分类号： G02B6/10 ,  G02B6/12

CPC分类号： G02B6/10 ,  G01N21/0303 ,  G01N2021/6482

摘要： Fluidic waveguides have inward surfaces or areas that face each other, separated by a channel region that can be covered. For example, an integrally formed channel component can include two walls parts and a connecting part, with inward surfaces on the wall parts and, extending between them, a base surface; a covering component's lower surface can also extend between the inward surfaces, bounding the channel region; other fluidic, electrical, and optical components can also be attached. In a stack, the covering component can cover the first channel component, and the lower base surface of each preceding channel component can cover the following channel component. An integrally formed body of light-transmissive material can have a surface that includes a waveguide's inward areas and a base area between them; a covering component can be mounted on areas adjacent the inward areas, providing an enclosed channel region.

摘要翻译： 流体波导具有彼此面对的内表面或区域，由可被覆盖的通道区域分开。 例如，整体形成的通道部件可以包括两个壁部分和连接部分，在壁部分上具有向内的表面，并且在它们之间延伸有一个基面; 覆盖部件的下表面也可以在限定通道区域的内表面之间延伸; 也可以连接其他流体，电和光学部件。 在堆叠中，覆盖部件可以覆盖第一通道部件，并且每个先前的通道部件的下基座表面可以覆盖随后的通道部件。 一体形成的透光材料体可以具有包括波导的向内区域和它们之间的基底区域的表面; 覆盖部件可以安装在邻近内部区域的区域上，从而提供封闭的通道区域。