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1.
公开(公告)号:US08039648B2
公开(公告)日:2011-10-18
申请号:US12485439
申请日:2009-06-16
申请人: Peter T. Czerney , Surbhi Desai , Matthias S. Wenzel , Boguslawa Dworecki , Frank G. Lehmann , Bernd G. Schweder , Wilhelm G. Frank
发明人: Peter T. Czerney , Surbhi Desai , Matthias S. Wenzel , Boguslawa Dworecki , Frank G. Lehmann , Bernd G. Schweder , Wilhelm G. Frank
IPC分类号: C07D311/08
CPC分类号: C07D405/12 , C07D311/94 , G01N33/533
摘要: Compounds used as dyes comparable to Alexa Fluor 350 dyes. The inventive compounds have high fluorescence quantum yield and high photostability. The dyes facilitate analysis of biological structures with enhanced sensitivity and selectivity.
摘要翻译: 用作染料的化合物与Alexa Fluor 350染料相当。 本发明化合物具有高荧光量子产率和高光稳定性。 染料促进生物结构的分析,增强灵敏度和选择性。
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公开(公告)号:US07745640B2
公开(公告)日:2010-06-29
申请号:US11566699
申请日:2006-12-05
申请人: Peter T. Czerney , Wilhelm G. Frank , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
发明人: Peter T. Czerney , Wilhelm G. Frank , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
IPC分类号: C07D209/02 , A61K31/40
CPC分类号: G01N33/582 , G01N33/533
摘要: Compounds, compositions, and methods for optical, including fluorescence optical, determinations useful in labelling biomolecules such as protein and deoxyribonucleic acid for their detection and quantitation. The compounds are diasteromeric cyanines with high hydrophilicity and other desirable properties.
摘要翻译: 用于光学的化合物,组合物和方法,包括用于标记生物分子如蛋白质和脱氧核糖核酸的荧光光学测定用于其检测和定量。 该化合物是具有高亲水性和其它所需性质的非对映体花青。
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3.
公开(公告)号:US20100317045A1
公开(公告)日:2010-12-16
申请号:US12485439
申请日:2009-06-16
申请人: Surbhi Desai , Boguslawa Dworecki , Matthias S. Wenzel , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Wilhelm G. Frank
发明人: Surbhi Desai , Boguslawa Dworecki , Matthias S. Wenzel , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Wilhelm G. Frank
IPC分类号: C12Q1/02 , C07D311/94
CPC分类号: C07D405/12 , C07D311/94 , G01N33/533
摘要: Compounds used as dyes comperable to Alexa Fluor 350 dyes. The inventive compounds have high fluorescence quantum yield and high photostability. The dyes facilitate analysis of biological structures with enhanced sensitivity and selectivity.
摘要翻译: 用作Alexa Fluor 350染料的染料的化合物。 本发明化合物具有高荧光量子产率和高光稳定性。 染料促进生物结构的分析,增强灵敏度和选择性。
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公开(公告)号:US07947506B2
公开(公告)日:2011-05-24
申请号:US11421883
申请日:2006-06-02
申请人: Brian David Wolf , Surbhi Desai , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
发明人: Brian David Wolf , Surbhi Desai , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
CPC分类号: G01N33/6839
摘要: A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.
摘要翻译: 配制为用于蛋白质检测,测定,定量等的荧光染料的羟基喹诺酮化合物。羟基喹诺酮化合物可以通过例如加入或除去硫酸根,改变烃链长度等来改变以产生更理想的性质,例如 增强的与碱性蛋白质的结合,增加的溶解度等。染料比商业上可获得的蛋白质污渍具有更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物上的蛋白质等。使用染料的方法 也被披露。
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公开(公告)号:US07553869B2
公开(公告)日:2009-06-30
申请号:US11460700
申请日:2006-07-28
申请人: Peter T. Czerney , Surbhi Desai , Frank G. Lehmann , Zakir S. Murtaza , Bernd G. Schweder , Matthias S. Wenzel , Brian David Wolf
发明人: Peter T. Czerney , Surbhi Desai , Frank G. Lehmann , Zakir S. Murtaza , Bernd G. Schweder , Matthias S. Wenzel , Brian David Wolf
IPC分类号: A01N43/16 , A61K31/35 , C07D311/02
CPC分类号: C07D491/052 , C07D311/56 , C07D493/04 , G01N27/44726 , G01N33/6827
摘要: Coumarine/chinolone and Martina-type compounds, compositions, and method of using the compounds and compositions for protein detection, assay, quantitation, etc. are disclosed. The composition, also referred to as a dye, has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc.
摘要翻译: 披露了使用该化合物和组合物进行蛋白质检测,测定,定量等的香豆素/氯诺酮和马丁纳型化合物,组合物和方法。 该组合物,也称为染料,具有比商业上可获得的蛋白质污渍更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物的蛋白质等。
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公开(公告)号:US20070281360A1
公开(公告)日:2007-12-06
申请号:US11421883
申请日:2006-06-02
申请人: Brian David Wolf , Surbhi Desai , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
发明人: Brian David Wolf , Surbhi Desai , Peter T. Czerney , Frank G. Lehmann , Bernd G. Schweder , Matthias S. Wenzel
IPC分类号: G01N33/00
CPC分类号: G01N33/6839
摘要: A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.
摘要翻译: 配制为用于蛋白质检测,测定,定量等的荧光染料的羟基喹诺酮化合物。羟基喹诺酮化合物可以通过例如加入或除去硫酸根,改变烃链长度等来改变以产生更理想的性质,例如 增强的与碱性蛋白质的结合,增加的溶解度等。染料比商业上可获得的蛋白质污渍具有更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物上的蛋白质等。使用染料的方法 也被披露。
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公开(公告)号:US20080026478A1
公开(公告)日:2008-01-31
申请号:US11460700
申请日:2006-07-28
申请人: Peter T. Czerney , Surbhi Desai , Frank G. Lehmann , Zakir S. Murtaza , Bernd G. Schweder , Matthias S. Wenzel , Brian David Wolf
发明人: Peter T. Czerney , Surbhi Desai , Frank G. Lehmann , Zakir S. Murtaza , Bernd G. Schweder , Matthias S. Wenzel , Brian David Wolf
IPC分类号: G01N33/50 , C07D409/02 , C07D405/02
CPC分类号: C07D491/052 , C07D311/56 , C07D493/04 , G01N27/44726 , G01N33/6827
摘要: Coumarine/chinolone and Martina-type compounds, compositions, and method of using the compounds and compositions for protein detection, assay, quantitation, etc. are disclosed. The composition, also referred to as a dye, has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc.
摘要翻译: 披露了使用该化合物和组合物进行蛋白质检测,测定,定量等的香豆素/氯诺酮和马丁纳型化合物,组合物和方法。 该组合物,也称为染料,具有比商业上可获得的蛋白质污渍更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物的蛋白质等。
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公开(公告)号:US09676787B2
公开(公告)日:2017-06-13
申请号:US14411447
申请日:2013-08-20
申请人: Greg Hermanson , Peter T. Czerney , Surbhi Desai , Matthias S. Wenzel , Frank G. Lehmann , Marie Christine Nlend
发明人: Greg Hermanson , Peter T. Czerney , Surbhi Desai , Matthias S. Wenzel , Frank G. Lehmann , Marie Christine Nlend
IPC分类号: A61K49/00 , C07D491/147 , G01N33/58 , C07D405/14 , C07D405/06 , C07D491/052 , C07D491/16 , C09B23/06 , C09B23/04 , C09B23/08 , C09B69/00
CPC分类号: C07D491/147 , A61K49/0021 , A61K49/0032 , A61K49/0054 , A61K49/0058 , C07D405/06 , C07D405/14 , C07D491/052 , C07D491/16 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/086 , C09B69/00 , G01N33/582
摘要: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds are conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
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公开(公告)号:US20170152267A9
公开(公告)日:2017-06-01
申请号:US14411447
申请日:2013-08-20
申请人: Greg Hermanson , Peter T. Czerney , Surbhi Desai , Matthias S. Wenzel , Frank G. Lehmann , Marie Christine Nlend
发明人: Greg Hermanson , Peter T. Czerney , Surbhi Desai , Matthias S. Wenzel , Frank G. Lehmann , Marie Christine Nlend
IPC分类号: C07D491/147 , G01N33/58 , C07D491/052 , A61K49/00 , C07D405/06 , C07D405/14
CPC分类号: C07D491/147 , A61K49/0021 , A61K49/0032 , A61K49/0054 , A61K49/0058 , C07D405/06 , C07D405/14 , C07D491/052 , C07D491/16 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/086 , C09B69/00 , G01N33/582
摘要: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds are conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
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公开(公告)号:US09365598B2
公开(公告)日:2016-06-14
申请号:US14526901
申请日:2014-10-29
申请人: Greg Hermanson , Peter T. Czerney , Surbhi Desai , Suk J. Hong , Matthias S. Wenzel , Boguslawa R. Dworecki , Frank G. Lehmann
发明人: Greg Hermanson , Peter T. Czerney , Surbhi Desai , Suk J. Hong , Matthias S. Wenzel , Boguslawa R. Dworecki , Frank G. Lehmann
IPC分类号: C07K16/00 , C07K14/00 , C07F9/535 , C07F9/6561 , C07F9/6558 , C07K1/13 , G01N33/58
CPC分类号: C07F9/6561 , C07F9/535 , C07F9/65583 , C07K1/13 , C07K14/00 , C07K16/00 , C07K2317/20 , G01N33/582
摘要: A phosphine derivative of DyLight dyes modified with ethylene glycol or (poly)ethylene glycol groups. In one embodiment, the compounds are useful in chemoselective ligation reactions.
摘要翻译: 用乙二醇或(聚)乙二醇基改性的DyLight染料的膦衍生物。 在一个实施方案中,所述化合物可用于化学选择性连接反应。
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