Protein detection and quantitation using hydroxyquinolone dyes
    8.
    发明授权
    Protein detection and quantitation using hydroxyquinolone dyes 有权
    蛋白质检测和定量使用羟基喹诺酮染料

    公开(公告)号:US07947506B2

    公开(公告)日:2011-05-24

    申请号:US11421883

    申请日:2006-06-02

    CPC分类号: G01N33/6839

    摘要: A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.

    摘要翻译: 配制为用于蛋白质检测,测定,定量等的荧光染料的羟基喹诺酮化合物。羟基喹诺酮化合物可以通过例如加入或除去硫酸根,改变烃链长度等来改变以产生更理想的性质,例如 增强的与碱性蛋白质的结合,增加的溶解度等。染料比商业上可获得的蛋白质污渍具有更高的灵敏度,并且可用于染色溶液中的蛋白质,凝胶上分离的蛋白质,转移到固体支持物上的蛋白质等。使用染料的方法 也被披露。