公开(公告)号：US20070092964A1

公开(公告)日：2007-04-26

申请号：US10570716

申请日：2004-09-01

申请人： Peter Wagner ,  Mark Scalf ,  Frank Zaugg

发明人： Peter Wagner ,  Mark Scalf ,  Frank Zaugg

IPC分类号： C12M1/34

CPC分类号： H01J49/0418 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/069 ,  B01L2300/0819 ,  Y10T436/11 ,  Y10T436/114165 ,  Y10T436/143333

摘要： The present invention provides methods and assemblies for ion detection of samples using a chip with elevated sample zones. The elevated sample zones provide a number of ion detection advantages over chips with non-elevated sample zones. Embodiments of the invention have a number of applications in drug discovery, environmental analyses for tracking and the identification of contaminants, target discovery and/or validation as well as in diagnostics in a clinical setting for staging or disease progression. In addition, the invention may also be used with research and clinical microarray systems and devices.

摘要翻译： 本发明提供了使用具有升高的样品区的芯片进行离子检测的方法和组件。 提升的样品区域提供了超过具有非升高样品区域的芯片的许多离子检测优点。 本发明的实施方案在药物发现，用于跟踪的环境分析和污染物鉴定，靶发现和/或验证以及用于分期或疾病进展的临床环境中的诊断中具有许多应用。 此外，本发明还可以用于研究和临床微阵列系统和装置。

公开(公告)号：US08765391B2

公开(公告)日：2014-07-01

申请号：US12859973

申请日：2010-08-20

申请人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

发明人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

IPC分类号： G01N33/00 ,  G01N33/543 ,  G01N33/553 ,  G01N1/18

CPC分类号： G01N33/54326 ,  G01N33/491 ,  G01N33/538 ,  G01N33/54313 ,  G01N33/56972

摘要： A method and kit for assaying a cell sample for the presence of at least a threshold number of cells of a given type are disclosed. The kit includes an assay device having a sample chamber for receiving the cell sample and an elongate collection chamber containing a selected-density and/or viscosity medium and having along its length, a plurality of cell-collection regions, and particles which are capable of specific attachment to cells of the selected cell type, and which are effective, when attached to the cells, to increase the density or magnetic susceptibility of the cells. In operation, particle-bound cells and particles in the cell sample are drawn through the elongate collection chamber under the influence of a gravitational or selected centrifugal or magnetic-field force until the particle-bound cells and particles completely fill successive cell-collection regions in the collection chamber. Indicia associated with at least one collection regions indicates a concentration of cells of the selected type effective to at least partially fill that collection region.

摘要翻译： 公开了用于测定细胞样品中存在至少一个给定类型的至少一个阈值数目的细胞的方法和试剂盒。 该试剂盒包括具有用于接收细胞样品的样品室和包含选定密度和/或粘度介质并且沿其长度具有多个细胞收集区域的细长收集室的测定装置和能够 特异性附着于所选择的细胞类型的细胞，并且当附着于细胞时是有效的，以增加细胞的密度或磁化率。 在操作中，细胞样品中的颗粒结合的细胞和颗粒在重力或选定的离心或磁场力的影响下被拉伸通过细长收集室，直到颗粒结合的细胞和颗粒完全填充连续的细胞收集区域 收集室。 与至少一个收集区域相关联的标记表示所选择类型的细胞的浓度有效至少部分地填充该收集区域。

公开(公告)号：US20090087925A1

公开(公告)日：2009-04-02

申请号：US11865555

申请日：2007-10-01

申请人： Peter Wagner ,  Frank Zaugg ,  Mark Mitchnick

发明人： Peter Wagner ,  Frank Zaugg ,  Mark Mitchnick

IPC分类号： G01N33/543 ,  B01J19/00 ,  C12M1/00 ,  G01N21/00 ,  G01N33/00

CPC分类号： G01N33/54366 ,  B01F5/061 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0059 ,  B01F2005/0621 ,  B01F2005/0636 ,  B01J2219/00605 ,  B01L3/502761 ,  B01L2200/148 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0472 ,  B01L2400/086 ,  G01N33/56972 ,  G01N33/581 ,  G01N33/6869 ,  G01N2021/6439

摘要： A system and method for determining the presence and/or concentration of one or more analytes in a sample that comprises a fluid, the system comprising a solid substrate comprising a sample inlet or inlets and one or more analyte determination flow paths, each analyte determination flow path comprising a defined beginning and a defined terminus and comprising at least one capture zone containing a capture agent for an analyte, the capture agent or agents being immobilized along a portion of the flow path or paths, the flow path or paths being designed so that the one or more analytes are depleted from the sample and bound in a non-linear manner to the portion of the flow path or paths containing immobilized capture agent or agents, producing an analyte depletion end region for each analyte between the beginning and the terminus of the analyte determination flow path.

摘要翻译： 一种用于确定包含流体的样品中一种或多种分析物的存在和/或浓度的系统和方法，所述系统包括固体基质，其包含样品入口或入口以及一个或多个分析物确定流路径，每个分析物确定流程 路径，其包含限定的起始点和限定的末端，并且包含至少一个包含分析物的捕获剂的捕获区域，所述捕获剂或试剂沿所述流动路径或路径的一部分被固定，所述流动路径或路径被设计成使得 一个或多个分析物从样品中耗尽，并以非线性方式结合到包含固定化捕获剂或试剂的流动路径或路径的部分，从而在每个分析物的起始和终点之间产生分析物耗尽末端区域 分析物确定流路。

公开(公告)号：US08304203B2

公开(公告)日：2012-11-06

申请号：US12681601

申请日：2008-12-05

申请人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

发明人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

IPC分类号： G01N33/00 ,  G01N7/00 ,  G01N1/18 ,  F04B19/00 ,  B01L3/00

CPC分类号： G01N33/54326 ,  G01N33/491 ,  G01N33/538 ,  G01N33/54313 ,  G01N33/56972

摘要： A method and kit for assaying a cell sample for the presence of at least a threshold number of cells of a given type are disclosed. The kit includes an assay device having a sample chamber for receiving the cell sample and an elongate collection chamber containing a selected-density and/or viscosity medium and having along its length, a plurality of cell-collection regions, and particles which are capable of specific attachment to cells of the selected cell type, and which are effective, when attached to the cells, to increase the density or magnetic susceptibility of the cells. In operation, particle-bound cells and particles in the cell sample are drawn through the elongate collection chamber under the influence of a gravitational or selected centrifugal or magnetic-field force until the particle-bound cells and particles completely fill successive cell-collection regions in the collection chamber. Indicia associated with at least one collection regions indicates a concentration of cells of the selected type effective to at least partially fill that collection region.

摘要翻译： 公开了用于测定细胞样品中存在至少一个给定类型的至少一个阈值数目的细胞的方法和试剂盒。 该试剂盒包括具有用于接收细胞样品的样品室和包含选定密度和/或粘度介质并且沿其长度具有多个细胞收集区域的细长收集室的测定装置和能够 特异性附着于所选择的细胞类型的细胞，并且当附着于细胞时是有效的，以增加细胞的密度或磁化率。 在操作中，细胞样品中的颗粒结合的细胞和颗粒在重力或选定的离心或磁场力的影响下被拉伸通过细长收集室，直到颗粒结合的细胞和颗粒完全填充连续的细胞收集区域 收集室。 与至少一个收集区域相关联的标记表示所选择类型的细胞的浓度有效至少部分地填充该收集区域。

公开(公告)号：US07998696B2

公开(公告)日：2011-08-16

申请号：US12329380

申请日：2008-12-05

申请人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

发明人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

IPC分类号： G01N33/00 ,  G01N7/00 ,  G01N1/18 ,  F04B19/00 ,  B01L3/00

CPC分类号： G01N33/54326 ,  G01N33/491 ,  G01N33/538 ,  G01N33/54313 ,  G01N33/56972

摘要： A method and kit for assaying a cell sample for the presence of at least a threshold number of cells of a given type are disclosed. The kit includes an assay device having a sample chamber for receiving the cell sample and an elongate collection chamber containing a selected-density and/or viscosity medium and having along its length, a plurality of cell-collection regions, and particles which are capable of specific attachment to cells of the selected cell type, and which are effective, when attached to the cells, to increase the density or magnetic susceptibility of the cells. In operation, particle-bound cells and particles in the cell sample are drawn through the elongate collection chamber under the influence of a gravitational or selected centrifugal or magnetic-field force until the particle-bound cells and particles completely fill successive cell-collection regions in the collection chamber. Indicia associated with at least one collection regions indicates a concentration of cells of the selected type effective to at least partially fill that collection region.

摘要翻译： 公开了用于测定细胞样品中存在至少一个给定类型的至少一个阈值数目的细胞的方法和试剂盒。 该试剂盒包括具有用于接收细胞样品的样品室和包含选定密度和/或粘度介质并且沿其长度具有多个细胞收集区域的细长收集室的测定装置和能够 特异性附着于所选择的细胞类型的细胞，并且当附着于细胞时是有效的，以增加细胞的密度或磁化率。 在操作中，细胞样品中的颗粒结合的细胞和颗粒在重力或选定的离心或磁场力的影响下被拉伸通过细长收集室，直到颗粒结合的细胞和颗粒完全填充连续的细胞收集区域 收集室。 与至少一个收集区域相关联的标记表示所选择类型的细胞的浓度有效至少部分地填充该收集区域。

公开(公告)号：US20100311085A1

公开(公告)日：2010-12-09

申请号：US12859973

申请日：2010-08-20

申请人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

发明人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

IPC分类号： G01N33/50 ,  C12M1/34

CPC分类号： G01N33/54326 ,  G01N33/491 ,  G01N33/538 ,  G01N33/54313 ,  G01N33/56972

摘要： A method and kit for assaying a cell sample for the presence of at least a threshold number of cells of a given type are disclosed. The kit includes an assay device having a sample chamber for receiving the cell sample and an elongate collection chamber containing a selected-density and/or viscosity medium and having along its length, a plurality of cell-collection regions, and particles which are capable of specific attachment to cells of the selected cell type, and which are effective, when attached to the cells, to increase the density or magnetic susceptibility of the cells. In operation, particle-bound cells and particles in the cell sample are drawn through the elongate collection chamber under the influence of a gravitational or selected centrifugal or magnetic-field force until the particle-bound cells and particles completely fill successive cell-collection regions in the collection chamber. Indicia associated with at least one collection regions indicates a concentration of cells of the selected type effective to at least partially fill that collection region.

摘要翻译： 公开了用于测定细胞样品中存在至少一个给定类型的至少一个阈值数目的细胞的方法和试剂盒。 该试剂盒包括具有用于接收细胞样品的样品室和包含选定密度和/或粘度介质并且沿其长度具有多个细胞收集区域的细长收集室的测定装置和能够 特异性附着于所选择的细胞类型的细胞，并且当附着于细胞时是有效的，以增加细胞的密度或磁化率。 在操作中，细胞样品中的颗粒结合的细胞和颗粒在重力或选定的离心或磁场力的影响下被拉伸通过细长收集室，直到颗粒结合的细胞和颗粒完全填充连续的细胞收集区域 收集室。 与至少一个收集区域相关联的标记表示所选择类型的细胞的浓度有效至少部分地填充该收集区域。

公开(公告)号：US20110207150A1

公开(公告)日：2011-08-25

申请号：US12681601

申请日：2008-12-05

申请人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

发明人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus-Munoz ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

IPC分类号： G01N33/53 ,  G01N33/569 ,  C12M1/34

CPC分类号： G01N33/54326 ,  G01N33/491 ,  G01N33/538 ,  G01N33/54313 ,  G01N33/56972

摘要： A method and kit for assaying a cell sample for the presence of at least a threshold number of cells of a given type are disclosed. The kit includes an assay device having a sample chamber for receiving the cell sample and an elongate collection chamber containing a selected-density and/or viscosity medium and having along its length, a plurality of cell-collection regions, and particles which are capable of specific attachment to cells of the selected cell type, and which are effective, when attached to the cells, to increase the density or magnetic susceptibility of the cells. In operation, particle-bound cells and particles in the cell sample are drawn through the elongate collection chamber under the influence of a gravitational or selected centrifugal or magnetic-field force until the particle-bound cells and particles completely fill successive cell-collection regions in the collection chamber. Indicia associated with at least one collection regions indicates a concentration of cells of the selected type effective to at least partially fill that collection region.

摘要翻译： 公开了用于测定细胞样品中存在至少一个给定类型的至少一个阈值数目的细胞的方法和试剂盒。 该试剂盒包括具有用于接收细胞样品的样品室和包含选定密度和/或粘度介质并且沿其长度具有多个细胞收集区域的细长收集室的测定装置和能够 特异性附着于所选择的细胞类型的细胞，并且当附着于细胞时是有效的，以增加细胞的密度或磁化率。 在操作中，细胞样品中的颗粒结合的细胞和颗粒在重力或选定的离心或磁场力的影响下被拉伸通过细长收集室，直到颗粒结合的细胞和颗粒完全填充连续的细胞收集区域 收集室。 与至少一个收集区域相关联的标记表示所选择类型的细胞的浓度有效至少部分地填充该收集区域。

公开(公告)号：US20110124130A1

公开(公告)日：2011-05-26

申请号：US12936318

申请日：2008-04-03

申请人： Peter Wagner ,  Frank Zaugg ,  Mark Mitchnik

发明人： Peter Wagner ,  Frank Zaugg ,  Mark Mitchnik

IPC分类号： G01N33/543

CPC分类号： G01N33/558 ,  G01N33/54366

摘要： A system and method for determining the presence and/or concentration of one or more analytes in a sample that comprises a fluid, the system comprising a substrate comprising a sample inlet or inlets and one or more analyte determination flow paths, each analyte determination flow path comprising a defined beginning and a defined terminus and comprising at least one capture zone containing a capture agent for an analyte, the capture agent or agents being immobilized along a portion of the flow path or paths, the flow path or paths being designed so that the one or more analytes are depleted from the sample and bound to the portion of the flow path or paths containing immobilized capture agent or agents, producing an analyte depletion end region for each analyte between the beginning and the terminus of the analyte determination flow path.

摘要翻译： 一种用于确定样品中一种或多种分析物在含有流体的存在和/或浓度的系统和方法，所述系统包括含有样品入口或入口以及一个或多个分析物确定流路的基底，每个分析物确定流路 包括限定的起始点和限定的末端，并且包含至少一个含有分析物的捕获剂的捕获区，所述捕获剂或试剂沿所述流动路径或路径的一部分被固定，所述流路或路径被设计成使得 一个或多个分析物从样品中消耗并且结合到含有固定化捕获剂或试剂的流动路径或路径的部分，为分析物确定流路的开始和终点之间的每个分析物产生分析物耗尽端区。

公开(公告)号：US07834314B2

公开(公告)日：2010-11-16

申请号：US12082149

申请日：2008-04-08

申请人： Peter Wagner ,  Mark A. Scalf ,  Frank Zaugg

发明人： Peter Wagner ,  Mark A. Scalf ,  Frank Zaugg

IPC分类号： H01J49/00 ,  C02F1/40 ,  B01L3/00

CPC分类号： H01J49/0418 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/069 ,  B01L2300/0819 ,  Y10T436/11 ,  Y10T436/114165 ,  Y10T436/143333

摘要： Methods and assemblies for ion detection in samples using a chip with elevated sample zones, also known as a “pillar chip.” Methods include analyzing such a sample by desorbing a sample from a chip, producing a described ion sample and detecting the same. The chip comprises a base having a surface and one or more structures protruding above the surface of the base. Each structure comprises a pillar and a sample zone, the latter containing a support material and the sample to be analyzed. Assemblies include a chip such as that described above and a conductive element that comprises an aperture of sufficient proportion to allow passage of a molecular ion and that is adapted to be at a different electrical potential than the base of the chip.

摘要翻译： 使用具有升高的样品区的芯片的样品中的离子检测方法和组件，也称为“柱芯片”。方法包括通过从芯片解吸样品来分析这样的样品，产生所述离子样品并进行检测。 芯片包括具有突出在基底表面上方的表面和一个或多个结构的基底。 每个结构包括柱和样品区，后者包含支撑材料和要分析的样品。 组件包括如上所述的芯片和包括足够比例的孔以允许分子离子通过并且适于处于与芯片的基极不同的电位的导电元件。

公开(公告)号：US20090148869A1

公开(公告)日：2009-06-11

申请号：US12329380

申请日：2008-12-05

申请人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

发明人： Frank Zaugg ,  Renee Tobias ,  Silvia McManus ,  Peter Kernen ,  Laurence Ruiz-Taylor ,  Peter Wagner

IPC分类号： G01N33/53 ,  C12Q1/06 ,  G01N33/569 ,  C12M1/00

CPC分类号： G01N33/54326 ,  G01N33/491 ,  G01N33/538 ,  G01N33/54313 ,  G01N33/56972

摘要： A method and kit for assaying a cell sample for the presence of at least a threshold number of cells of a given type are disclosed. The kit includes an assay device having a sample chamber for receiving the cell sample and an elongate collection chamber containing a selected-density and/or viscosity medium and having along its length, a plurality of cell-collection regions, and particles which are capable of specific attachment to cells of the selected cell type, and which are effective, when attached to the cells, to increase the density or magnetic susceptibility of the cells. In operation, particle-bound cells and particles in the cell sample are drawn through the elongate collection chamber under the influence of a gravitational or selected centrifugal or magnetic-field force until the particle-bound cells and particles completely fill successive cell-collection regions in the collection chamber. Indicia associated with at least one collection regions indicates a concentration of cells of the selected type effective to at least partially fill that collection region.

摘要翻译： 公开了用于测定细胞样品中存在至少一个给定类型的至少一个阈值数目的细胞的方法和试剂盒。 该试剂盒包括具有用于接收细胞样品的样品室和包含选定密度和/或粘度介质并且沿其长度具有多个细胞收集区域的细长收集室的测定装置和能够 特异性附着于所选择的细胞类型的细胞，并且当附着于细胞时是有效的，以增加细胞的密度或磁化率。 在操作中，细胞样品中的颗粒结合的细胞和颗粒在重力或选定的离心或磁场力的影响下被拉伸通过细长收集室，直到颗粒结合的细胞和颗粒完全填充连续的细胞收集区域 收集室。 与至少一个收集区域相关联的标记表示所选择类型的细胞的浓度有效至少部分地填充该收集区域。