公开(公告)号：US20090059207A1

公开(公告)日：2009-03-05

申请号：US12281906

申请日：2007-03-02

申请人： Philippe Nerin ,  Benoit Merchez ,  Jean-Philippe Gineys ,  Didier Lefevre

发明人： Philippe Nerin ,  Benoit Merchez ,  Jean-Philippe Gineys ,  Didier Lefevre

IPC分类号： G01N21/62

CPC分类号： G01N15/1434 ,  G01N15/1427 ,  G01N15/1459 ,  G01N2015/1486

摘要： The invention relates to a device and to a method for measuring photoluminescence in a fluid present in a measurement vessel. According to the invention, the fluid in the measurement vessel simultaneously receives at least two excitation beams coming from two optical systems. The optical systems are positioned so that their axes form between them a non-zero obtuse angle other than 180° around the measurement vessel. A measurement of light emission is deduced according to the invention from coupling data obtained from emission beams picked up simultaneously by the pickup elements. The optical systems are also positioned in such a manner that there exists at least one partial overlap beam between the excitation beam from the source of a first optical system and the emission beam picked up by the pickup element of a second optical system. The device is also provided with at least one extinction pickup element in the vicinity of at least one of the sources for picking up light at the excitation wavelength in the partial overlap beam, a measurement of absorbance and/or diffraction being deduced from data obtained from the light picked up by the extinction pickup element.

摘要翻译： 本发明涉及一种用于测量存在于测量容器中的流体中的光致发光的装置和方法。 根据本发明，测量容器中的流体同时接收来自两个光学系统的至少两个激发光束。 光学系统被定位成使得它们的轴线在它们之间形成围绕测量容器的180°以外的非零钝角。 根据本发明，从由拾取元件同时拾取的发射光束获得的耦合数据推导出发光测量。 光学系统也被定位成使得在来自第一光学系统的光源的激发光束和由第二光学系统的拾取元件拾取的发射光束之间存在至少一个部分重叠光束。 该装置还在至少一个源附近设置有至少一个消光拾取元件，用于拾取部分重叠光束中的激发波长的光，从根据以下方面获得的数据推导出吸光度和/或衍射的测量值： 被灭光元件拾取的光线。

公开(公告)号：US20080283754A1

公开(公告)日：2008-11-20

申请号：US11912055

申请日：2006-04-14

申请人： Philippe Nerin ,  Didier Lefevre

发明人： Philippe Nerin ,  Didier Lefevre

IPC分类号： G01J5/02

CPC分类号： G01N21/6428 ,  G01N15/1434 ,  G01N15/147 ,  G01N2015/1477 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2021/6419 ,  G01N2021/6421

摘要： The invention concerns a device (DA) for analyzing microscopic elements, comprising firstly a measuring space (CM) for microscopic elements to be analyzed, secondly at least one source (S) delivering conjugated rays at the measuring space (CM), having at least two different analyzing wavelengths and designed to interact with the microscopic elements in the measuring space (CM) to form interacting rays, thirdly coding means (M) for encoding the rays upstream of the measuring space (CM) with different codes, fourthly optical filtering means (FO) for selectively filtering the interacting rays of fluorescence and/or diffusion depending on their wavelength, fifthly detecting means (DE, DF) for transforming into electric signals part at least of the interacting rays from the measuring space (CM), and sixthly analyzing means (MA) including decoding means (DRE, DRF) for decoding the electric signals to enable data representing the analyzed microscopic elements to be determined.

摘要翻译： 本发明涉及一种用于分析微观元素的装置（DA），其首先包括用于要分析的微观元素的测量空间（CM），其次，在测量空间（CM）处传送共轭射线的至少一个源（S），至少具有 两个不同的分析波长，并设计成与测量空间（CM）中的微观元件相互作用以形成相互作用的射线;第三编码装置（M），用于以不同的代码编码测量空间（CM）上游的光线;第四，光学滤波装置 （FO），用于根据其波长选择性地过滤荧光和/或扩散的相互作用的光线;第五检测装置（DE，DF），用于将来自测量空间（CM）的至少相互作用的光线部分转换成电信号;以及第六 分析装置（MA），包括用于解码电信号的解码装置（DRE，DRF），以使得能够确定表示所分析的微观元素的数据。

公开(公告)号：US07777869B2

公开(公告)日：2010-08-17

申请号：US11912055

申请日：2006-04-14

申请人： Philippe Nerin ,  Didier Lefevre

发明人： Philippe Nerin ,  Didier Lefevre

IPC分类号： G01N33/48

CPC分类号： G01N21/6428 ,  G01N15/1434 ,  G01N15/147 ,  G01N2015/1477 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2021/6419 ,  G01N2021/6421

摘要： The invention concerns a device (DA) for analyzing microscopic elements, comprising firstly a measuring space (CM) for microscopic elements to be analyzed, secondly at least one source (S) delivering conjugated rays at the measuring space (CM), having at least two different analyzing wavelengths and designed to interact with the microscopic elements in the measuring space (CM) to form interacting rays, thirdly coding means (M) for encoding the rays upstream of the measuring space (CM) with different codes, fourthly optical filtering means (FO) for selectively filtering the interacting rays of fluorescence and/or diffusion depending on their wavelength, fifthly detecting means (DE, DF) for transforming into electric signals part at least of the interacting rays from the measuring space (CM), and sixthly analyzing means (MA) including decoding means (DRE, DRF) for decoding the electric signals to enable data representing the analyzed microscopic elements to be determined.

摘要翻译： 本发明涉及一种用于分析微观元素的装置（DA），其首先包括用于要分析的微观元素的测量空间（CM），其次，在测量空间（CM）处传送共轭射线的至少一个源（S），至少具有 两个不同的分析波长，并设计成与测量空间（CM）中的微观元件相互作用以形成相互作用的射线;第三编码装置（M），用于以不同的代码编码测量空间（CM）上游的光线;第四，光学滤波装置 （FO），用于根据其波长选择性地过滤荧光和/或扩散的相互作用的光线;第五检测装置（DE，DF），用于将来自测量空间（CM）的至少相互作用的光线部分转换成电信号;以及第六 分析装置（MA），包括用于解码电信号的解码装置（DRE，DRF），以使得能够确定表示所分析的微观元素的数据。

公开(公告)号：US20070111276A1

公开(公告)日：2007-05-17

申请号：US11647268

申请日：2006-12-29

申请人： Didier Lefevre ,  Sylvie Veriac ,  Henri Champseix

发明人： Didier Lefevre ,  Sylvie Veriac ,  Henri Champseix

IPC分类号： G01N1/30

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25125 ,  Y10T436/2525

摘要： The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.

摘要翻译： 本发明涉及用于鉴定和计数样品中生物细胞的试剂和方法。 该试剂包含选自能够特异性裂解样品中给定类型的细胞的浓度的至少一种洗涤剂的细胞裂解剂和能够标记剩余未溶解细胞的细胞内核酸的染色剂。 特别适用于使用基于流式细胞术的自动化分析系统鉴定和计数细胞的应用。

公开(公告)号：US5138181A

公开(公告)日：1992-08-11

申请号：US602952

申请日：1990-10-26

申请人： Didier Lefevre ,  Henri Champseix ,  Serge Champseix

发明人： Didier Lefevre ,  Henri Champseix ,  Serge Champseix

IPC分类号： G01N33/49 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14

CPC分类号： G01N15/12 ,  G01N2015/0065 ,  G01N2015/008 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/1413

摘要： An apparatus is disclosed for counting and determining at least one leucocytic sub-population, comprising a case for injecting a flow of sample to be analyzed inside a measurement tank through which a light beam passes, comprising internal and external nozzles for sleeving the sample by means of a pressurized liquid. Electrodes for the resistivity measurements of the leucocytic sub-population are component elements of the injection case. Units for collecting and processing the signals delivered by the optical sensor and the electrodes are also provided.

摘要翻译： 公开了一种用于计数和确定至少一个白细胞亚群的装置，包括用于将待分析样品流注入光束通过的测量槽内的壳体，其包括用于通过装置套管样品的内部和外部喷嘴 的加压液体。 白细胞亚群的电阻率测量的电极是注射盒的组成元素。 还提供了用于收集和处理由光学传感器和电极传送的信号的单元。

公开(公告)号：US5730941A

公开(公告)日：1998-03-24

申请号：US653325

申请日：1996-05-24

申请人： Didier Lefevre ,  Bernard Kress

发明人： Didier Lefevre ,  Bernard Kress

IPC分类号： G01N21/64 ,  G01N15/14 ,  G01N21/47 ,  G01N33/49

CPC分类号： G01N15/14

摘要： It includes a measuring space (8) comprising a constrained passage (15) for the fluid, a monochromatic light source (31) delivering a beam (F1) to optical elements (6, 32, 34) including a first diffractive optical element (32) having a diffractive network (38) predetermined as a function, on the one hand, of the characteristics of the source and, on the other hand, of chosen geometrical and spectral characteristics for the illumination of the constrained passage, and suitable for inducing fluorescence of at least part of the fluid, and first (19-20), second (34-36) and third (40) optoelectronic elements intended to collect, respectively, a transmitted beam, fluorescence and a diffracted beam, and suitable for delivering to an analysis unit (24) signals relating to the contents of the beams. At least one of the beams emanates from a second diffractive optical element (42) having a second diffractive optical network which is predetermined as a function of a frequential and geometrical transfer function of at least one of the optoelectronic elements.

摘要翻译： 它包括测量空间（8），其包括用于流体的约束通道（15），将光束（F1）传送到包括第一衍射光学元件（32,32）的光学元件（6,32,34）的单色光源（31） ）具有作为功能的预定的衍射网络（38），一方面是源的特性，另一方面，对于受限通道的照明的选择的几何和光谱特性，并且适于诱导荧光 至少部分流体和第一（19-20），第二（34-36）和第三（40）光电子元件，分别收集透射束，荧光和衍射光束，并且适于传送到 分析单元（24）与波束的内容有关的信号。 至少一个光束从具有第二衍射光网络的第二衍射光学元件（42）发出，该第二衍射光学网络被预定为至少一个光电元件的频率和几何传递函数的函数。

公开(公告)号：US08080383B2

公开(公告)日：2011-12-20

申请号：US12158597

申请日：2006-12-05

申请人： Francis Lacombe ,  Francis Belloc ,  Sylvie Veriac ,  Didier Lefevre

发明人： Francis Lacombe ,  Francis Belloc ,  Sylvie Veriac ,  Didier Lefevre

IPC分类号： G01N33/567 ,  G01N21/76

CPC分类号： G01N15/1456 ,  G01N33/5005 ,  G01N33/5094 ,  G01N33/56966 ,  G01N33/582 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2800/52 ,  Y10T436/101666 ,  Y10T436/13

摘要： A subject of the invention is a method for discriminating between and counting at least two populations of biological elements carrying specific characteristics, optionally present in a sample. The invention allows the clear and unambiguous detection of at least three populations of biological elements by the use of only two detection means, which means that at least two populations of biological elements are detected by one and the same detection means. The invention can be carried out if three different probes are used, each recognizing and becoming fixed to one of the populations of biological elements to be detected, each of the probes being itself rendered detectable by a different marker, two of said markers having two emission spectra having at least one common part (overlapping emission spectra) and the third having an emission spectrum having essentially no part in common with the other two (non-overlapping spectrum).

摘要翻译： 本发明的主题是用于区分并计数至少两个携带特定特征的生物元件群体的方法，任选存在于样品中。 本发明允许通过仅使用两个检测手段来清楚和明确地检测至少三个生物元素群体，这意味着至少两个生物元素群被同一检测手段检测。 如果使用三种不同的探针，每种识别并变得固定在待检测的生物元素群体之中，则可以进行本发明，每个探针本身可由不同的标记物检测，两个所述标记物具有两个发射 光谱具有至少一个公共部分（重叠发射光谱），第三个光谱具有基本上没有与其它两个（非重叠光谱）共同的部分的发射光谱。

公开(公告)号：US08008029B2

公开(公告)日：2011-08-30

申请号：US11572817

申请日：2005-07-06

申请人： Didier Lefevre

发明人： Didier Lefevre

IPC分类号： G01N33/567 ,  G01N27/00 ,  G01N21/00

CPC分类号： G01N15/147 ,  G01N2015/1037 ,  G01N2015/1477 ,  Y10T436/10 ,  Y10T436/101666

摘要： The invention concerns a method for differentiating and counting cellular components present in a biological fluid sample comprising a primary cytological analysis step typically implemented by a flow cytometry equipment (1) to obtain a set of primary results enabling the set of cellular components of the sample to be differentiated and counted in different populations; and a complementary step of cytological analysis of a particular type of cellular components, based on an identified cellular peculiarity, to obtain complementary results enabling at least one cell population or subpopulation of the sample to be differentiated and counted for identification of said cellular peculiarity. The invention is applicable in particular to hematological analyses.

摘要翻译： 本发明涉及用于区分和计数存在于生物流体样品中的细胞成分的方法，其包括通常由流式细胞术设备（1）实施的主要细胞学分析步骤，以获得使得该组样品的细胞组分能够 在不同的人群中进行区分和计数; 以及基于鉴定的细胞特性的特定类型的细胞组分的细胞学分析的补充步骤，以获得使至少一个细胞群体或样品亚群被分化并计数以鉴定所述细胞特性的互补结果。 本发明特别适用于血液学分析。

公开(公告)号：US20090311678A1

公开(公告)日：2009-12-17

申请号：US12158597

申请日：2006-12-05

申请人： Francis Lacombe ,  Francis Belloc ,  Sylvie Veriac ,  Didier Lefevre

发明人： Francis Lacombe ,  Francis Belloc ,  Sylvie Veriac ,  Didier Lefevre

IPC分类号： C12Q1/68 ,  C12Q1/02 ,  G01N33/53

CPC分类号： G01N15/1456 ,  G01N33/5005 ,  G01N33/5094 ,  G01N33/56966 ,  G01N33/582 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2800/52 ,  Y10T436/101666 ,  Y10T436/13

摘要： A subject of the invention is a method for discriminating between and counting at least two populations of biological elements carrying specific characteristics, optionally present in a sample. The invention allows the clear and unambiguous detection of at least three populations of biological elements by the use of only two detection means, which means that at least two populations of biological elements are detected by one and the same detection means. The invention can be carried out if three different probes are used, each recognizing and becoming fixed to one of the populations of biological elements to be detected, each of the probes being itself rendered detectable by a different marker, two of said markers having two emission spectra having at least one common part (overlapping emission spectra) and the third having an emission spectrum having essentially no part in common with the other two (non-overlapping spectrum).

摘要翻译： 本发明的主题是用于区分并计数至少两个携带特定特征的生物元件群体的方法，任选存在于样品中。 本发明允许通过仅使用两个检测手段来清楚和明确地检测至少三个生物元素群体，这意味着至少两个生物元素群被同一检测手段检测。 如果使用三种不同的探针，每种识别和变得固定在待检测的生物元素群体之一中，则可以进行本发明，每个探针本身可由不同的标记物检测，两个所述标记物具有两个发射 光谱具有至少一个公共部分（重叠发射光谱），第三个光谱具有基本上没有与其它两个（非重叠光谱）共同的部分的发射光谱。

公开(公告)号：US06319470B1

公开(公告)日：2001-11-20

申请号：US09372600

申请日：1999-08-12

申请人： Didier Lefevre ,  Ghislain Morel ,  Henri Champseix ,  Serge Champseix ,  Roger Le Comte

发明人： Didier Lefevre ,  Ghislain Morel ,  Henri Champseix ,  Serge Champseix ,  Roger Le Comte

IPC分类号： G01N3510

CPC分类号： G01N1/312 ,  G01N1/2813 ,  G01N35/00029 ,  G01N2035/00039 ,  G01N2035/00138

摘要： A device for automatic preparation of blood smears on plates comprises a storage unit for stacked new plates, an extractor unit to extract a plate from the stack, a depositing unit. To deposit a drop of blood on a plate, a spreading unit, to spread a drop of blood and produce a blood smear, a marking unit, comprising a marking head which can place means of identification on a reserved area of the plate, a drying unit, which can dry the blood smear, a coloring unit), which can color the blood smear, an output unit to unload the plate, and transfer means, to displace the plate between the aforementioned units, according to a pre-determined path.

摘要翻译： 用于在板上自动准备血液涂片的装置包括用于堆叠的新板的存储单元，从堆叠提取板的提取单元，沉积单元。 为了将一滴血液沉积在平板，铺展单元上，以散布一滴血液并产生血液涂片，一个标记单元，包括一个标记头，该标记头可以在该板的保留区域上放置识别手段，一个干燥 根据预先确定的路径，能够使血液涂片干燥的单元，着色单元），其可以使血液涂片着色，输出单元卸载板和传送装置，以在前述单元之间移动板。