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    Broad host range small plasmid rings as cloning vehicles
    4.
    发明授权
    Broad host range small plasmid rings as cloning vehicles 失效
    广泛的宿主范围小质粒环作为克隆载体

    公开(公告)号：US4374200A

    公开(公告)日：1983-02-15

    申请号：US147563

    申请日：1980-05-08

    Applicant: Ronald H. Olsen

    Inventor： Ronald H. Olsen

    IPC: C12N15/09 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12N15/52 ,  C12N15/74 ,  C12N15/78 ,  C12P13/06 ,  C12P13/08 ,  C12P13/12 ,  C12P19/34 ,  C12R1/01 ,  C12R1/065 ,  C12R1/19 ,  C12R1/38 ,  C12R1/385 ,  C12N1/00 ,  C12P21/00 ,  C12P21/02 ,  C12R1/22

    CPC classification number: C12P13/06 ,  C12N15/52 ,  C12N15/74 ,  C12N15/78 ,  C12P13/08 ,  C12P13/12

    Abstract: The present invention relates to novel, broad bacterial host range small plasmid deoxyribonucleic acid rings which serve as cloning vehicles for DNA fragments, particularly those separated from other plasmid rings or from chromosones, recombined with the small plasmid rings and to the processes for recombining the plasmid rings and to processes for transferring them between host bacteria. In particular, the present invention relates to the aggregate plasmid ring RP1/pRO1600, to pRO1600 and plasmid ring derivatives thereof, particularly including pRO1601; pRO1613 and pRO1614, all of which are carried for reference purposes in Pseudomonas aeruginosa ATCC 15692 (also known as strain PAO1c) and are on deposit at the Northern Regional Research Laboratories (NRRL) of the U. S. Dept. of Agriculture at Peoria, Ill. The plasmid ring RP1 (also known as R1822) is deposited in Pseudomonas aeruginosa NRRL-B-12123 (and is a known plasmid ring). The pRO1600 portion of the aggregate is a new plasmid ring. The novel small plasmid rings are particularly useful for recombinant genetic manipulation wherein the DNA fragments are introduced into the plasmid rings to produce useful, cloneable characteristics in the host bacterium, particularly chemical generating characteristics.

    Abstract translation: 本发明涉及新的广泛的细菌宿主范围的小质粒脱氧核糖核酸环，其用作用于DNA片段的克隆载体，特别是与其他质粒环或与小质粒环重组的染色体分离的那些，以及用于重组质粒的方法 环和转移宿主细菌之间的过程。 特别地，本发明涉及聚合质粒环RP1 / pRO1600，pRO1600及其质粒环衍生物，特别是包括pRO1601; pRO1613和pRO1614，所有这些都用于铜绿假单胞菌ATCC15692（也称为菌株PAO1c）中的参考目的，并且存在于美国伊利诺州皮奥里亚州美国农业部北部地区研究实验室（NRRL）上。 将质粒环RP1（也称为R1822）沉积在铜绿假单胞菌NRRL-B-12123中（并且是已知的质粒环）。 聚集体的pRO1600部分是新的质粒环。 新颖的小质粒环特别可用于重组遗传操作，其中将DNA片段引入质粒环以在宿主细菌中产生有用的可克隆特征，特别是化学产生特性。

    Antiammonia azotobacter and the use thereof
    7.
    发明授权
    Antiammonia azotobacter and the use thereof 失效
    安非他酮固氮菌及其用途

    公开(公告)号：US4877739A

    公开(公告)日：1989-10-31

    申请号：US252578

    申请日：1988-09-30

    Applicant: Zhen H. Yang

    Inventor： Zhen H. Yang

    IPC: A21D2/08 ,  A23K1/00 ,  A23K1/16 ,  A23L1/30 ,  A61K35/74 ,  A61P3/06 ,  A61P35/00 ,  C12N1/20 ,  C12P1/04 ,  C12R1/065

    CPC classification number: C12N1/20 ,  A21D2/08 ,  A23K10/16 ,  A61K35/74 ,  C12P1/04 ,  Y10S435/831

    Abstract: This invention relates to a group of autogenic antiammonia azotobacter represented by 851 yellow. It can utilize low price starch medium. This culture fluid of azotobacter can be used for manufacturing single cell proteins rich in Se, Zn, V.sub.E, V.sub.B, V.sub.K, anticancer and antiaging tonic medicines. It also can be used for manufacturing bacteria manure, eel and animal forage, additive, antiseptic and binder.

    Abstract translation: 本发明涉及由851黄代表的一组自发性抗氨固氮素。 它可以利用低价淀粉介质。 固氮菌培养液可用于制造富含Se，Zn，VE，VB，VK，抗癌和抗衰老补品的单细胞蛋白。 它还可用于制造细菌粪便，鳗鱼和动物饲料，添加剂，防腐剂和粘合剂。

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