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公开(公告)号：US20200163553A1
公开(公告)日：2020-05-28
申请号：US16773533
申请日：2020-01-27
申请人： ROBERT ALFANO , LINGYAN SHI
发明人： ROBERT ALFANO , LINGYAN SHI
摘要： A label free single or multi-photon optical excitation fluorescence spectroscopy for measuring the differences between the levels of fluorophores from tryptophan, collagen, reduced nicotinamide adenine dinucleotide (NADH), and flavins exist in brain samples from a of Alzheimer's disease (AD) and in normal (N) brain samples with label-free fluorescence spectroscopy. Relative quantities of these molecules are shown by the spectral profiles of the AD and N brain samples at excitation wavelengths and multi photons about 266 nm, 300 nm, 400 nm and 500 nm. The emission spectral profile levels of tryptophan and flavin were much higher in AD samples, while collagen emission levels were slightly lower and NADH levels were much lower in AD samples. These results yield a new optical method for detection of biochemical differences in animals and humans for Alzheimer's disease. These molecules in AD and N tissues and cells can be excited by 1PEF, 2PEF, 3PEF, 4 PEF using fs and ps pulses
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公开(公告)号：US20180238738A1
公开(公告)日：2018-08-23
申请号：US15904089
申请日：2018-02-23
申请人： ROBERT ALFANO , LINGYAN SHI
发明人： ROBERT ALFANO , LINGYAN SHI
CPC分类号： G01J3/4412 , A61K49/0013 , A61K49/006 , G01J3/10 , G01J3/44 , H01S3/30
摘要： A nonlinear optical process on increasing the signal in SRS microscope by Resonant Stimulated Raman Scattering (RSRS) combines both RRS and SRS nonlinear processes, in absorber and host such a β-carotene-methanol solution, Flavins, or other key absorbers in tissues. The observed effect of enhanced RSRS in small signal gain is attributed to RR process in absorber β-carotene that transferring excess vibrations to host methanol from anharmonic vibrational interactions between the solute β-carotene in resonance with the solvent methanol vibrations. SRS microscopy signals are improved in RSRS microscope for imaging vibrational states in lipids CH2 and proteins CH3 in cancer tissues from RR in Flavins or other native chromophores in tissue and for applications in other areas of neuroscience and biomedicine, potentially enhancing signals in the RSRS microscope by 2 to 1000 times.
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公开(公告)号：US20180078142A1
公开(公告)日：2018-03-22
申请号：US15397431
申请日：2017-01-03
申请人： ROBERT ALFANO , LINGYAN SHI
发明人： ROBERT ALFANO , LINGYAN SHI
IPC分类号： A61B5/00 , G01N33/483 , G01N21/64
CPC分类号： A61B5/0071 , A61B5/4076 , A61B10/0045 , A61B10/02 , A61B2010/0077 , G01N21/645 , G01N21/6486 , G01N33/483 , G01N33/6896 , G01N2021/6484 , G01N2800/2814 , G01N2800/2821 , G01N2800/2835
摘要： A label free single or multi-photon optical spectroscopy for measuring the differences between the levels of fluorophores from tryptophan, collagen, reduced nicotinamide adenine dinucleotide (NADH), and flavins exist in brain samples from a of Alzheimer's disease (AD) and in normal (N) brain samples with label-free fluorescence spectroscopy. Relative quantities of these molecules are shown by the spectral profiles of the AD and N brain samples at excitation wavelengths 266 nm, 300 nm, and 400 nm. The emission spectral profile levels of tryptophan and flavin were much higher in AD samples, while collagen emission levels were slightly lower and NADH levels were much lower in AD samples. These results yield a new optical method for detection of biochemical differences in animals and humans for Alzheimer's disease.
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公开(公告)号：US20170153435A1
公开(公告)日：2017-06-01
申请号：US15409303
申请日：2017-01-18
申请人： ROBERT ALFANO , LINGYAN SHI
发明人： ROBERT ALFANO , LINGYAN SHI
CPC分类号： G02B21/0064 , A61B5/0066 , A61B18/20 , A61B2017/00508 , A61B2018/1807 , A61B2018/2065 , A61N5/0622 , A61N2005/0659 , A61N2005/067 , G01N33/4833 , G02B21/0032 , G02B21/0084 , G02F1/3515 , G02F1/3536 , G02F1/355 , G02F1/3551 , G02F1/365 , G02F2001/3528 , G06N10/00
摘要： Supercontinuum (SC) (˜400 nm to ˜2500 nm) and a microscope produce enhanced microscopic images on sub-micron to cm scale of linear (χ1) and nonlinear (χ2, χ3, χ4 . . . ) processes via resonance including linear absorption, SHG, THG, SRG, SRL, SRS. 2PEF, 3PEF, 4PEF, and inverse Raman in a microscope for 2D and 3D imaging. Images and processes in 2D and 3D arise from electronic and vibrational resonances transitions in biological and medical tissues, cells, condensed matter applications. Resonant Stimulated Raman Scattering (RSRS) is proposed to improve vibrational imaging of biomaterials by using part of SC. Quantum mechanical processes from SC for 2 and 4 photons to improve resolution and imaging using entangled photons are described. The addition of time measuring instrument like a Streak camera and the scattering coefficient μs′ can be mapped to create images of tissue and biomaterial in 5D: Space (3D), Time, and Wavelength.
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公开(公告)号：US20210080382A1
公开(公告)日：2021-03-18
申请号：US16990623
申请日：2020-08-11
申请人： ROBERT ALFANO
发明人： ROBERT ALFANO , SANDRA MAMANI , LINGYAN SHI
摘要： A super class of polarized transverse vector vortex photon beams patterns are mathematically represented here, which are Majorana-like among them are the radial and azimuthal Laguerre-Gaussian, hybrid π-vector beams, and Airy beams. These optical beams are consider spin-orbit coupled beams based on OAM and SAM parts of light. A Majorana photon is a photon that is identical to its anti-photon. It has within itself both chirality, right and left-handed twist in polarization (SAM) and wavefront (OAM). Applications using Majorana photons improve optical deeper imaging, higher resolution imaging, Nonlinear Optics effects (SHG, SRS, SC), optical communication in free space and fibers, quantum computer as basic qubit, and entanglement for security.
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