公开(公告)号：US20070295244A1

公开(公告)日：2007-12-27

申请号：US11629990

申请日：2005-06-23

申请人： Ralf Himmelreich ,  Sabine Werner ,  Claudia Fritz ,  Hideo Takahashi ,  Masaki Shimojoh ,  Toshihiko Akamatsu ,  Keisuke Iriyama

发明人： Ralf Himmelreich ,  Sabine Werner ,  Claudia Fritz ,  Hideo Takahashi ,  Masaki Shimojoh ,  Toshihiko Akamatsu ,  Keisuke Iriyama

IPC分类号： C08K9/00 ,  C09C1/36 ,  C09C3/06

CPC分类号： C09C1/3692 ,  C01P2006/12 ,  C08K9/06 ,  C09C1/3661 ,  C09C1/3669 ,  C09C1/3684 ,  Y10T428/2993 ,  Y10T428/2995

摘要： The invention provides titanium dioxide pigments for plastics resin compositions which are excellent in dispersibility, light resistance, and weather resistance and little cause surface imperfection. In particular, the invention provides a titanium dioxide pigment suitable for general-purpose plastics resin compositions which can attain excellent dispersion and lacing resistance even when blended in a high concentration; a titanium dioxide pigment suitable for engineering plastics resin compositions which can attain dispersion properties, silver streaking resistance, inhibition of reduction in molecular weight, and thermal discoloration resistance at high levels; a process for the production of the pigments; and resin compositions containing the pigments. A titanium dioxide pigment for plastics resin compositions, characterized in that each titanium dioxide particle has a dense hydrous silica coat covering its surface and an organic compound coat formed on the silica coat not through an interlayer.

摘要翻译： 本发明提供了分散性，耐光性和耐候性优异且表面不完美的塑料树脂组合物用二氧化钛颜料。 特别地，本发明提供了适用于通用塑料树脂组合物的二氧化钛颜料，即使以高浓度混合也能获得优异的分散性和耐蕾曲性; 适用于工程塑料树脂组合物的二氧化钛颜料，其可以获得分散性能，银耐划伤性，抑制分子量降低和高水平的耐热变色性; 一种生产颜料的方法; 和含有颜料的树脂组合物。 一种用于塑料树脂组合物的二氧化钛颜料，其特征在于，每个二氧化钛颗粒具有覆盖其表面的致密含水二氧化硅涂层和在二氧化硅涂层上形成的有机复合涂层，而不是通过中间层。

公开(公告)号：US20080166703A1

公开(公告)日：2008-07-10

申请号：US10577721

申请日：2004-10-20

申请人： Ralf Himmelreich ,  Sabine Werner ,  Claudia Fritz

发明人： Ralf Himmelreich ,  Sabine Werner ,  Claudia Fritz

IPC分类号： C12Q1/68 ,  C07H1/00

CPC分类号： C12N15/1017 ,  C07H1/08

摘要： The invention relates to a rapid method for isolating nucleic acid from a nucleic acid source, wherein the nucleic acid source is lysed in the absence of a chaotropic salt and in the absence of an alcohol. The lysate is subsequently filtered through a porous matrix consisting of a material based on silica or of a silica coated material, which binds the nucleic acid in the absence of a chaotropic salt and in the absence of an alcohol. Finally, the nucleic acid is eluted from the porous matrix by an aqueous buffer solution. Furthermore, this invention relates to a test kit in order to isolate the nucleic acid.

摘要翻译： 本发明涉及从核酸源分离核酸的快速方法，其中核酸源在不存在离液序列盐的情况下裂解，并且在不存在醇的情况下裂解。 裂解物随后通过由基于二氧化硅或二氧化硅涂覆的材料的材料组成的多孔基质过滤，所述材料在不存在离液序列盐的情况下结合核酸，并且在不存在醇的情况下。 最后，通过缓冲液水溶液从多孔基质中洗脱核酸。 此外，本发明涉及用于分离核酸的测试试剂盒。

公开(公告)号：US08076069B2

公开(公告)日：2011-12-13

申请号：US12477813

申请日：2009-06-03

申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymers, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilization of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 包括a）多胺，b）氨基酸和寡聚和多肽，c）包含这些含氮化合物的含氮杂环化合物，包括均相杂聚物，d）R1R2NR3型胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺，f ）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

公开(公告)号：US20100323363A1

公开(公告)日：2010-12-23

申请号：US12862156

申请日：2010-08-24

申请人： Christian KORFHAGE ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

发明人： Christian KORFHAGE ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

IPC分类号： C12Q1/68

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡聚物和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括均匀的杂多聚物，d）R1R2NR3型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）碳酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

公开(公告)号：US07838233B2

公开(公告)日：2010-11-23

申请号：US11910247

申请日：2006-04-01

申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

IPC分类号： C12Q1/68 ,  C12P21/06 ,  C07K1/00 ,  C07K14/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-older heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides In particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡聚物和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括同质杂多酸，d）R1R2NR3类型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5 - 烷基和芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供一种用于裂解生物样品的方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

公开(公告)号：US20120130061A1

公开(公告)日：2012-05-24

申请号：US13061916

申请日：2009-09-02

申请人： Ralf Himmelreich ,  Sabine Werner

发明人： Ralf Himmelreich ,  Sabine Werner

IPC分类号： C07H1/06 ,  C07H21/04 ,  C09K3/00 ,  C07H1/08 ,  C07C43/13 ,  C07H21/00 ,  C07H21/02

CPC分类号： C12N15/1003 ,  C12N15/1006

摘要： The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples and biological materials. The present invention further relates to a kit for carrying out the method of the invention.

摘要翻译： 本发明涉及通过从含核酸的样品和生物材料中洗脱核酸来分离和纯化核酸的方法。 本发明还涉及用于实施本发明方法的试剂盒。

公开(公告)号：US20080132694A1

公开(公告)日：2008-06-05

申请号：US10567351

申请日：2004-08-16

申请人： Ralf Himmelreich ,  Sabine Werner

发明人： Ralf Himmelreich ,  Sabine Werner

IPC分类号： C07H21/04

CPC分类号： C12N15/1013

摘要： The present invention provides a method for isolating biopolymers, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from an aqueous solution utilizing magnetic particles, particularly silica magnetic particles. The method of the present invention involves forming a complex of the magnetic particles and the biopolymer in a mixture of said particles and said aqueous solution, comprising a salt and an additive; separating the complex from the mixture by a magnetic force, and eluting the biopolymer from the complex, wherein advantageously no substantial clustering of said magnetic particles occurs during the performance of the method. In a preferred embodiment the method of the invention is performed as an automated process.

摘要翻译： 本发明提供一种利用磁性颗粒，特别是二氧化硅磁性颗粒从水溶液中分离生物聚合物，特别是核酸，如DNA或RNA或DNA和RNA的混合分子的方法。 本发明的方法包括在所述颗粒和所述水溶液的混合物中形成磁性颗粒和生物聚合物的复合物，其包含盐和添加剂; 通过磁力将复合物与混合物分离，并从复合物中洗脱生物聚合物，其中在实施该方法期间有利地不发生所述磁性颗粒的基本聚集。 在优选实施例中，本发明的方法作为自动化过程进行。

公开(公告)号：US08778842B2

公开(公告)日：2014-07-15

申请号：US11883071

申请日：2006-02-06

申请人： Ralf Himmelreich ,  Roland Fabis ,  Christoph Erbacher ,  Sabine Werner ,  Bernd Springer

发明人： Ralf Himmelreich ,  Roland Fabis ,  Christoph Erbacher ,  Sabine Werner ,  Bernd Springer

IPC分类号： C12Q1/68 ,  C08G73/02

CPC分类号： C08F8/32 ,  C12Q1/689

摘要： The present invention provides copolymers that facilitate nucleic acid analysis, compositions that comprise such copolymers, and methods for making or using such copolymers.

摘要翻译： 本发明提供促进核酸分析的共聚物，包含这种共聚物的组合物，以及制备或使用这些共聚物的方法。

公开(公告)号：US20110224419A1

公开(公告)日：2011-09-15

申请号：US13061928

申请日：2009-09-02

申请人： Ralf Himmelreich ,  Sabine Werner

发明人： Ralf Himmelreich ,  Sabine Werner

IPC分类号： C07H1/06 ,  C12N9/50 ,  C09K109/00 ,  C08L79/00 ,  C08L35/06

CPC分类号： C12N15/1006

摘要： The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials. The present invention further relates to a kit for carrying out the method of the invention.

摘要翻译： 本发明涉及通过从含核酸的样品和生物材料中洗脱核酸来分离和纯化核酸的方法。 本发明还涉及用于实施本发明方法的试剂盒。

公开(公告)号：US08624020B2

公开(公告)日：2014-01-07

申请号：US13061928

申请日：2009-09-02

申请人： Ralf Himmelreich ,  Sabine Werner

发明人： Ralf Himmelreich ,  Sabine Werner

IPC分类号： C07H21/00

CPC分类号： C12N15/1006

摘要： The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials, using a wash buffer comprising an alcohol having 1 to 3 carbon atoms and at least one further solvent selected from the group consisting of alkane diols and alkane triols having 2 to 6 carbon atoms, monocarboxylic acid esters and dicarboxylic acid diesters having 2 to 6 carbon atoms in the acidic component and 1 to 4 carbon atoms in the alcoholic component; (poly)ethylene glycols and ether derivatives and ester derivatives thereof, and poly(4-styrene sulfonic acid-co-maleic acid) sodium salt solution. The present invention further relates to a kit for carrying out the method of the invention.

摘要翻译： 本发明涉及通过使用含有1至3个碳原子的醇和至少一种其它溶剂的洗涤缓冲液从核酸样品和生物材料中洗脱核酸来分离和纯化核酸的方法 选自烷烃二醇和具有2至6个碳原子的烷烃三醇，在酸性组分中具有2至6个碳原子的二羧酸二酯和醇组分中的1至4个碳原子的单羧酸酯和二羧酸二酯; （聚）乙二醇及其醚衍生物及其酯衍生物，以及聚（4-苯乙烯磺酸 - 共 - 马来酸）钠盐溶液。 本发明还涉及用于实施本发明方法的试剂盒。