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公开(公告)号：US4710464A
公开(公告)日：1987-12-01
申请号：US655184
申请日：1984-09-27
IPC分类号： C12N15/67 , C12N15/70 , C12N15/76 , C12P19/34 , C07H21/04 , C12N1/00 , C12N1/16 , C12N1/18 , C12N1/20 , C12N15/00 , C12P21/00
摘要： The present transcription terminators are DNA sequences characterized by their ability to terminate the transcription of DNA sequences. The present sequences are portable, have a variety of restriction endonuclease sites and are useful in controlling the expression in hosts of DNA sequences coding for prokaryotic, eukaryotic and viral proteins and polypeptides.
摘要翻译： 本转录终止子是以其终止DNA序列转录的能力为特征的DNA序列。 本发明的序列是便携式的，具有多种限制性内切核酸酶位点，并且可用于控制编码原核，真核和病毒蛋白质和多肽的DNA序列的宿主中的表达。
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公开(公告)号：US5190871A
公开(公告)日：1993-03-02
申请号：US364959
申请日：1989-06-12
申请人： Karen L. Cox , Stuart A. Kuhstoss , R. Nagaraja Rao , Mark A. Richardson , Brigitte E. Schoner , Eugene T. Seno
发明人： Karen L. Cox , Stuart A. Kuhstoss , R. Nagaraja Rao , Mark A. Richardson , Brigitte E. Schoner , Eugene T. Seno
摘要： The present invention provides a method for transforming an actinomycete with an integrating vector which has the advantages of high transformation rates into a broad host range, site-specific integration, and stable maintenance without antibiotic selection. Also provided are methods for the increased production of antibiotics and for the production of hybrid antibiotics.
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3.发明授权Transcription terminator and related recombinant DNA vectors and transformants 失效转录终止子和相关重组DNA载体和转化体
公开(公告)号：US5093252A
公开(公告)日：1992-03-03
申请号：US515538
申请日：1990-04-30
摘要： A transcription terminator is disclosed comprising a DNA sequence capable of functioning as a transcription terminator in a microorganism of the order Actinomycetales. These transcription terminators are functional in both gram-positive and gram-negative microorganisms, especially Streptomyces ambofaciens. Additionally, a series of recombinant DNA cloning vectors suitable for isolating the disclosed transcription terminators are set forth.
摘要翻译： 公开了一种转录终止子，其包含能够作为放线菌纲的微生物中的转录终止子的DNA序列。 这些转录终止子在革兰氏阳性和革兰氏阴性微生物，特别是链霉菌（Streptomyces ambofaciens）中均有功能。 另外，阐述了一系列用于分离所公开的转录终止子的重组DNA克隆载体。
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4.发明授权Method of using bacteriophage lambda p.sub.1 promoter to produce a functional polypeptide in streptomyces 失效使用噬菌体λp1启动子在链霉菌中产生功能性多肽的方法
公开(公告)号：US4766066A
公开(公告)日：1988-08-23
申请号：US655181
申请日：1984-09-27
IPC分类号： C12N15/09 , C12N1/20 , C12N9/00 , C12N15/73 , C12N15/76 , C12P21/00 , C12R1/465 , C12N15/00 , C12P19/34
CPC分类号： C12N15/73 , C12N15/76 , Y10S435/886 , Y10S435/889 , Y10S435/896
摘要： The invention relates to a method and cloning vehicle for the expression of a functional polypeptide in Streptomyces. A recombinant DNA cloning vehicle was genetically engineered to bring the expression of the neomycin phosphotransferase gene under the control of the Escherichia coli bacteriophage .lambda.p.sub.L promoter.
摘要翻译： 本发明涉及用于在链霉菌中表达功能性多肽的方法和克隆载体。 基因工程改造重组DNA克隆载体，使新霉素磷酸转移酶基因的表达在大肠杆菌噬菌体λpL启动子的控制下。
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公开(公告)号：US5945320A
公开(公告)日：1999-08-31
申请号：US804198
申请日：1997-02-21
申请人： Stanley G. Burgett , Stuart A. Kuhstoss , Ramachandra N. Rao , Mark A. Richardson , Paul R. Rosteck, Jr.
发明人： Stanley G. Burgett , Stuart A. Kuhstoss , Ramachandra N. Rao , Mark A. Richardson , Paul R. Rosteck, Jr.
IPC分类号： C12N15/09 , C07K14/195 , C07K14/41 , C12N9/00 , C12N15/52 , C12P17/08 , C12P19/62 , C12P21/02 , C12N15/63 , C12N15/76
摘要： A DNA molecule isolated from Streptomyces ambofaciens encodes the multi-functional proteins which direct the synthesis of the polyketide platenolide.
摘要翻译： 从链霉菌（Streptomyces ambofaciens）分离的DNA分子编码引导聚酮化合物底胶的合成的多功能蛋白质。
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公开(公告)号：US5876991A
公开(公告)日：1999-03-02
申请号：US804227
申请日：1997-02-21
IPC分类号： C12N15/09 , C12N9/00 , C12N15/00 , C12N15/52 , C12P17/08 , C12P19/62 , C12R1/465 , C12R1/54 , C12N15/62 , C12N15/76
摘要： A DNA molecule isolated from Streptomyces fradiae encodes the multi-functional proteins which direct the synthesis of the polyketide tylactone.
摘要翻译： 从Streptomyces fradiae分离的DNA分子编码引导聚酮化物内酯的合成的多功能蛋白质。
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