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15 条记录 (耗时 0.045 秒)

    Multiply-substituted protease variants
    5.
    发明申请
    Multiply-substituted protease variants 有权
    多重取代的蛋白酶变体

    公开(公告)号：US20050221461A1

    公开(公告)日：2005-10-06

    申请号：US10500943

    申请日：2003-01-16

    申请人： Ayrookaran Poulose ,  David Estell ,  James Kellis Jr ,  Richard Bott

    发明人： Ayrookaran Poulose ,  David Estell ,  James Kellis Jr ,  Richard Bott

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C11D3/386 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/50 ,  C12N9/52 ,  C12N9/54 ,  C12N9/56 ,  C12N15/57

    CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38609 ,  C12Y304/21062

    摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or re-combinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.

    摘要翻译： 公开了包含衍生自天然存在或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了包含在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的位置上的至少一个另外的取代的另外的变体。

    Continuous production of lightweight composite beams
    6.
    发明授权
    Continuous production of lightweight composite beams 有权
    连续生产轻质复合梁

    公开(公告)号：US06264781B1

    公开(公告)日：2001-07-24

    申请号：US09240400

    申请日：1999-01-29

    申请人： Richard Bott

    发明人： Richard Bott

    IPC分类号： B32B500

    CPC分类号： B29D99/0003 ,  B29C53/58 ,  B29C70/525 ,  E04C3/29 ,  Y10T156/1067 ,  Y10T156/1087 ,  Y10T156/1741

    摘要： An apparatus and process for continuously producing composite structural beams is disclosed. The process includes a first step of forming a tubular element from fibers of composite material, a second step of separating the tubular element into longitudinally extensive, circumferentially separated, corner caps, a third step of securing sandwich panels between adjacent corner caps to form a tubular beam, a fourth step of shaping the tubular beam, and a fifth step of curing the shaped tubular beam. Also disclosed is an exemplary beam constructed in accordance with the process of the present invention.

    摘要翻译： 公开了连续生产复合结构梁的装置和方法。 该方法包括从复合材料的纤维形成管状元件的第一步骤，将管状元件分离成纵向广泛的周向分离的角帽的第二步骤;将夹层板固定在相邻角盖之间以形成管状的第三步骤 梁，成形管状梁的第四步骤，以及固化成形管状梁的第五步骤。 还公开了根据本发明的方法构造的示例性光束。

    Synthesis and use of glycodendrimer reagents
    7.
    发明申请
    Synthesis and use of glycodendrimer reagents 审中-公开
    糖苷配基试剂的合成和使用

    公开(公告)号：US20050272670A1

    公开(公告)日：2005-12-08

    申请号：US10629976

    申请日：2003-07-30

    申请人： Benjamin Davis ,  John Jones ,  Richard Bott

    发明人： Benjamin Davis ,  John Jones ,  Richard Bott

    IPC分类号： A61K47/48 ,  C07H5/10 ,  C07K14/36 ,  C12N1/21 ,  A61K31/70 ,  C08G63/48 ,  C08G63/91

    CPC分类号： B82Y5/00 ,  A61K47/51 ,  A61K47/665 ,  A61K47/6898 ,  C07H5/10 ,  C07K14/36

    摘要： The present invention relates to a chemically modified mutant protein including a cysteine residue substituted for a residue other than cysteine n a precursor protein, the substituted cysteine residue being subsequently modified by reacting the cysteine residue with a glycosylated thiosulfonate. Also a method of producing the chemically modified mutant protein is provided. The present invention also realtes to a glycosylated methanethiosulfonate. Another aspect of the present invention is a method of modifying the functional characteristics of a protein including providing a protein and reacting the protein with a glycosylated methanethiosulfonate reagent under conditions effective to produce a glycoprotein with altered functional characteristics as compared to the protein. In addition, the present invention relates to methods of determining the structure-function relationships of chemically modified mutant proteins. The present invention also relates to synthetic methods for producing thio-glycoses, the thio-glycoses so produced, and to methods for producing glycodendrimer reagents.

    摘要翻译： 本发明涉及一种化学修饰的突变蛋白，其包含半胱氨酸残基，其取代了半胱氨酸以外的残基而不是前体蛋白，随后通过半胱氨酸残基与糖基化硫代磺酸酯反应来修饰取代的半胱氨酸残基。 还提供了一种生产化学修饰的突变蛋白的方法。 本发明还涉及糖基化甲磺酸盐。 本发明的另一方面是一种改变蛋白质的功能特征的方法，包括提供蛋白质并使蛋白质与糖基化甲磺酸盐试剂在与蛋白质相比产生具有改变的功能特征的糖蛋白的条件下反应。 此外，本发明涉及确定化学修饰的突变蛋白的结构 - 功能关系的方法。 本发明还涉及用于制备硫代糖糖，如此制备的硫代糖糖的合成方法，以及制备糖苷配基体试剂的方法。

    Enzyme multimer and process of producing same
    10.
    发明申请
    Enzyme multimer and process of producing same 有权
    酶多聚体及其生产方法

    公开(公告)号：US20060084160A1

    公开(公告)日：2006-04-20

    申请号：US11021209

    申请日：2004-12-22

    申请人： Richard Bott ,  Christian Paech ,  Shan Wu

    发明人： Richard Bott ,  Christian Paech ,  Shan Wu

    IPC分类号： C12N11/18 ,  C12N9/54

    CPC分类号： C12N9/96 ,  C11D3/386 ,  C12N9/54 ,  D06M16/003 ,  D21C5/005

    摘要： An enzyme multimer is provided which comprises a plurality of monomer units including a first monomer unit having enzymatic activity and a second monomer unit having enzymatic activity, wherein each of the first monomer unit and the second monomer unit comprise a cysteine residue and the cysteine residues of each monomer unit are covalently bound to each other through a disulfide bond to covalently attach the first monomer unit to the second monomer unit.

    摘要翻译： 提供了一种酶多聚体，其包含多个单体单元，其包含具有酶活性的第一单体单元和具有酶活性的第二单体单元，其中第一单体单元和第二单体单元中的每一个包含半胱氨酸残基和半胱氨酸残基 每个单体单元通过二硫键共价键合以将第一单体单元共价连接到第二单体单元上。