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    Four-helical bundle protein zsig81
    1.
    发明授权
    Four-helical bundle protein zsig81 失效
    四螺旋束蛋白zsig81

    公开(公告)号：US06531576B1

    公开(公告)日：2003-03-11

    申请号：US09585228

    申请日：2000-06-01

    申请人： Christopher S. Piddington ,  James W. West ,  Richard D. Holly ,  Steven K. Burkhead

    发明人： Christopher S. Piddington ,  James W. West ,  Richard D. Holly ,  Steven K. Burkhead

    IPC分类号： C07K14475

    CPC分类号： C07K14/47 ,  C07K14/475

    摘要： This present invention is directed to polypeptide and polynucleotide molecules that encode a four-helical bundle cytokine. The cytokine has been designated zsig81, and has restricted expression in primarily heart, lung and liver. zsig81 has been shown to stimulate proliferation of hematopoietic cells and will be useful expansion of these cells, as well as conditions associated with hematopoietic cells. The invention is directed to antibodies and methods of making zsig81 polypeptides, as well.

    摘要翻译： 本发明涉及编码四螺旋束细胞因子的多肽和多核苷酸分子。 细胞因子被指定为zsig81，并且主要限制在心脏，肺和肝中的表达。 已经显示zsig81刺激造血细胞的增殖，并且这些细胞的有用扩张以及与造血细胞相关的病症。 本发明还涉及制备zsig81多肽的抗体和方法。

    Methods for preparing polynucleotides encoding orphan receptor ligands
    2.
    发明授权
    Methods for preparing polynucleotides encoding orphan receptor ligands 失效
    编制孤儿受体配体的多核苷酸的方法

    公开(公告)号：US5705349A

    公开(公告)日：1998-01-06

    申请号：US490803

    申请日：1995-06-15

    申请人： Richard D. Holly ,  Steven K. Burkhead

    发明人： Richard D. Holly ,  Steven K. Burkhead

    IPC分类号： A61K35/12 ,  C07K14/47 ,  C07K14/705 ,  C07K14/715 ,  C12N5/0787 ,  G01N33/50 ,  C12N15/12 ,  C12N15/19

    CPC分类号： G01N33/502 ,  C07K14/47 ,  C07K14/705 ,  C07K14/715 ,  C12N5/0642 ,  G01N33/5005 ,  G01N33/5008 ,  A61K2035/124 ,  C12N2501/145 ,  C12N2501/22

    摘要： Methods for obtaining cells that produce a ligand for an orphan receptor and methods for preparing polynucleotide molecules that encode ligands for orphan receptors are disclosed. The methods utilize growth factor-dependent parent cells that are transfected with a DNA construct encoding an orphan receptor. The transfected cells are exposed to mutagenizing conditions, and the mutagenized cells are cultured under conditions in which cell survival is dependent upon autocrine growth factor production. Progeny cells are recovered and screened to identify those that produce a ligand for the orphan receptor. Polynucleotide molecules encoding the ligand can be prepared from the identified cells.

    摘要翻译： 公开了获得产生孤儿受体配体的细胞的方法和制备编码孤儿受体配体的多核苷酸分子的方法。 该方法利用用编码孤儿受体的DNA构建体转染的生长因子依赖性亲本细胞。 将转染的细胞暴露于诱变条件下，并且在细胞存活取决于自分泌生长因子产生的条件下培养诱变的细胞。 回收和筛选后代细胞以鉴定产生孤儿受体配体的那些。 可以从鉴定的细胞制备编码配体的多核苷酸分子。

    Method for preparing orphan receptor ligands
    3.
    发明授权
    Method for preparing orphan receptor ligands 失效
    制备孤儿受体配体的方法

    公开(公告)号：US5541085A

    公开(公告)日：1996-07-30

    申请号：US250859

    申请日：1994-05-27

    申请人： Richard D. Holly ,  Steven K. Burkhead

    发明人： Richard D. Holly ,  Steven K. Burkhead

    IPC分类号： C12N15/19 ,  A61K35/12 ,  A61K38/19 ,  C07K14/47 ,  C07K14/705 ,  C07K14/715 ,  C12N1/19 ,  C12N5/0787 ,  C12N5/10 ,  C12N15/81 ,  C12N15/85 ,  C12P21/02 ,  G01N33/50 ,  C12N15/12

    CPC分类号： G01N33/5008 ,  C07K14/47 ,  C07K14/705 ,  C07K14/715 ,  C12N5/0642 ,  A61K2035/124 ,  C12N2501/145 ,  C12N2501/22

    摘要： Methods for obtaining cells that produce a ligand for an orphan receptor and methods for preparing polynucleotide molecules that encode ligands for orphan receptors are disclosed. The methods utilize growth factor-dependent parent cells that are transfected with a DNA construct encoding an orphan receptor. The transfected cells are exposed to mutagenizing conditions, and the mutagenized cells are cultured under conditions in which cell survival is dependent upon autocrine growth factor production. Progeny cells are recovered and screened to identify those that produce a ligand for the orphan receptor. Polynucleotide molecules encoding the ligand can be prepared from the identified cells.

    摘要翻译： 公开了获得产生孤儿受体配体的细胞的方法和制备编码孤儿受体配体的多核苷酸分子的方法。 该方法利用用编码孤儿受体的DNA构建体转染的生长因子依赖性亲本细胞。 将转染的细胞暴露于诱变条件下，并且在细胞存活取决于自分泌生长因子产生的条件下培养诱变的细胞。 回收和筛选后代细胞以鉴定产生孤儿受体配体的那些。 可以从鉴定的细胞制备编码配体的多核苷酸分子。

    Four-helical bundle protein zsig81
    4.
    发明授权
    Four-helical bundle protein zsig81 失效
    四螺旋束蛋白zsig81

    公开(公告)号：US07223564B2

    公开(公告)日：2007-05-29

    申请号：US10315379

    申请日：2002-12-09

    申请人： Christopher S. Piddington ,  James W. West ,  Richard D. Holly ,  Steven K. Burkhead

    发明人： Christopher S. Piddington ,  James W. West ,  Richard D. Holly ,  Steven K. Burkhead

    IPC分类号： C12N15/16 ,  C12N15/63 ,  C12N5/00 ,  C07K14/475 ,  C07K4/12

    CPC分类号： C07K14/47 ,  C07K14/475

    摘要： This present invention is directed to polypeptide and polynucleotide molecules that encode a four-helical bundle cytokine. The cytokine has been designated zsig81, and has restricted expression in primarily heart, lung and liver. zsig81 has been shown to stimulate proliferation of hematopoietic cells and will be useful expansion of these cells, as well as conditions associated with hematopoietic cells. The invention is directed to antibodies and methods of making zsig81 polypeptides, as well.

    摘要翻译： 本发明涉及编码四螺旋束细胞因子的多肽和多核苷酸分子。 细胞因子被指定为zsig81，并且主要限制在心脏，肺和肝中的表达。 已经显示zsig81刺激造血细胞的增殖，并且这些细胞的有用扩张以及与造血细胞相关的病症。 本发明还涉及制备zsig81多肽的抗体和方法。