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公开(公告)号：US5030240A
公开(公告)日：1991-07-09
申请号：US932717
申请日：1986-11-19
申请人： Richard J. Wiersema , Anna G. Stanislowski , Gregory L. Gray , Ayrookaram J. Poulose , Scott D. Power
发明人： Richard J. Wiersema , Anna G. Stanislowski , Gregory L. Gray , Ayrookaram J. Poulose , Scott D. Power
IPC分类号： C12P7/00 , C11D3/20 , C11D3/386 , C11D3/39 , C11D3/395 , C11D3/60 , C11D7/42 , C11D7/54 , C12N9/20 , C12N15/00 , C12P7/40 , C12P7/62 , C12R1/40 , D06L4/40
CPC分类号： D06L3/11 , C11D3/2093 , C11D3/38627 , C11D3/39 , C11D3/391 , C11D3/3945 , C12N9/20
摘要： An enzymatic perhydrolysis system, useful for bleaching, has a novel enzyme, a substrate, and a source of hydrogen peroxide, and provides in situ formation of peracid in aqueous solution. The substrate is selected for enzyme catalyzed reaction, and preferably is an acylglycerol with two or three fatty acid chains. The enzyme is hydrolytically and perhydrolytically active even in the presence of anionic surfactants, has lipase activity, and is isolatable from Pseudomonas putida ATCC 53552.
摘要翻译： 可用于漂白的酶促过水解系统具有新型酶，底物和过氧化氢源，并且在水溶液中原位形成过酸。 选择底物用于酶催化反应，并且优选是具有两个或三个脂肪酸链的酰基甘油。 该酶即使在阴离子表面活性剂存在下也具有水解和全水解活性，具有脂肪酶活性，并且可从恶臭假单胞菌ATCC 53552中分离。
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公开(公告)号：US5389536A
公开(公告)日：1995-02-14
申请号：US705052
申请日：1991-05-23
CPC分类号： C12N9/18
摘要： A substantially enzymatically pure hydrolase is provided which is secreted by and isolatable from Pseudomonas mendocina ATCC 53552. Cloning the gene expressing the hydrolase into a suitable expression vector and culturing, such as fermenting the E. coli strain JM101 harboring a plasmid designated pSNtacII, has been found to provide surprisingly high yields of the hydrolase.
摘要翻译： 提供了基本上酶的纯水解酶，其由门氏假单胞菌ATCC 53552分泌和分离。将表达水解酶的基因克隆到合适的表达载体中并培养，例如发酵含有命名为pSNtacII的质粒的大肠杆菌JM101 发现提供令人惊讶的高产率的水解酶。
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3.发明授权
公开(公告)号：US5093257A
公开(公告)日：1992-03-03
申请号：US431705
申请日：1989-11-01
申请人： Gregory L. Gray
发明人： Gregory L. Gray
IPC分类号： C12N15/09 , C12N9/28 , C12N9/52 , C12N9/54 , C12N15/00 , C12N15/62 , C12P21/00 , C12P21/02 , C12R1/07 , C12R1/19
CPC分类号： C12N9/2417 , C12N15/62 , C12N9/54 , C07K2319/00 , C07K2319/02
摘要： Novel circular vectors containing a recplicable DNA sequence and DNA sequences encoding all or part of at least two distinct parental polypeptides are disclosed. Such vectors are used in novel processes utilizing in vivo recombination to produce recombined circular vectors containing said replicable DNA sequences and hybrid DNA sequences comprising: (1) a first DNA sequence encoding the amino-terminal portion of a hybrid polypeptide corresponding to a first part of a first parental polypeptide sequence and (2) a second DNA sequence encoding a carboxy-terminal portion of said hybrid polypeptide corresponding to a first part of a second parental polypeptide sequence. The hybrid DNA sequences of such recombined circular vectors can exprress novel hybrid polypeptides such as hybrid enzymes in general and in particular hybrid amylases and proteases. Various other processes are disclosed to isolate the recombined circular vector containing said hybrid DNA sequences.
摘要翻译： 公开了含有可重复DNA序列的新型圆形载体和编码至少两种不同亲本多肽的全部或部分的DNA序列。 这样的载体用于利用体内重组的新方法中以产生含有可复制DNA序列和杂交DNA序列的重组环状载体，所述杂交DNA序列包含：（1）编码杂合多肽的氨基末端部分的第一DNA序列，其对应于第一部分 第一亲本多肽序列和（2）编码对应于第二亲本多肽序列的第一部分的所述杂合多肽的羧基末端部分的第二DNA序列。 这种重组环状载体的杂交DNA序列可以发挥新的杂合多肽，如通常的杂交酶，特别是杂交淀粉酶和蛋白酶。 公开了各种其它方法来分离含有所述杂交DNA序列的重组环状载体。
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4.发明授权Recombinant procaryotic cell containing correctly processed human growth hormone 失效含有正确加工的人生长激素的重组原核细胞
公开(公告)号：US4859600A
公开(公告)日：1989-08-22
申请号：US186493
申请日：1988-04-26
CPC分类号： C12N15/78 , C07K14/61 , C12N15/70 , Y10S930/12 , Y10S930/30
摘要： The production of mature hGH in E coli and Pseudomonas strains transformed by a plasmid which encodes pre hGH (comprising the signal polypeptide and the hormone itself) is described. These prokaryotes process the pre-hGH to cleave the signal sequence and, thereby, produce mature hGH.
摘要翻译： 描述了通过编码前hGH（包括信号多肽和激素本身）的质粒转化的大肠杆菌和假单胞菌菌株中的成熟hGH的产生。 这些原核生物处理前hGH以切割信号序列，从而产生成熟的hGH。
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公开(公告)号：US5364770A
公开(公告)日：1994-11-15
申请号：US413010
申请日：1989-09-25
IPC分类号： C12N1/15 , C12N1/19 , C12N9/34 , C12N9/58 , C12N9/64 , C12N15/62 , C12N15/80 , C12N15/09 , C12N15/56
CPC分类号： C12N9/2428 , C12N15/62 , C12N15/80 , C12N9/58 , C12N9/6481 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/61
摘要： Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.
摘要翻译： 公开了用于从丝状真菌表达和分泌异源多肽的新型载体。 这些载体用于表达和分泌这些异源多肽的新方法中。 用于转化丝状真菌以表达和分泌异源多肽的载体包括编码异源多肽的DNA序列和编码在给定丝状真菌中在分泌系统中有功能的信号序列的DNA序列，并且可操作地连接到 编码异源多肽的序列。 这样的信号序列可以是通常与异源多肽相关的信号序列，或者可以来自其他来源。 载体还可以含有编码启动子序列的DNA序列，其由丝状真菌功能识别，并且可操作地连接于编码信号序列的DNA序列。 优选地，功能性多腺苷酸化序列可操作地连接到编码异源多肽的DNA序列的3'末端。 上述载体中的每一种用于转化丝状真菌的新方法，其中表达编码信号序列和异源多肽的DNA序列。 由此合成的多肽此后由丝状真菌分泌。
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公开(公告)号：US5578463A
公开(公告)日：1996-11-26
申请号：US284942
申请日：1994-08-02
IPC分类号： C12N1/15 , C12N1/19 , C12N9/34 , C12N9/58 , C12N9/64 , C12N15/62 , C12N15/80 , C12N15/09 , C12N15/56
CPC分类号： C12N9/2428 , C12N15/62 , C12N15/80 , C12N9/58 , C12N9/6481 , C07K2319/00 , C07K2319/02 , C07K2319/50 , C07K2319/61
摘要： Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.
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公开(公告)号：US4963495A
公开(公告)日：1990-10-16
申请号：US658342
申请日：1984-10-05
CPC分类号： C12N15/70 , C07K14/24 , C07K14/61 , C12N15/625 , C07K2319/02 , C07K2319/034 , C07K2319/75
摘要： Direct linkage of DNA encoding prokaryotic signals such as E. coli enterotoxin with DNA encoding mature eukaryotic proteins followed by transformation and culture of bacterial hosts characterized by a periplasmic space, yields substantial amounts of periplasmic mature protein.
摘要翻译： 编码原核信号的DNA如大肠杆菌肠毒素与编码成熟真核生物蛋白的DNA直接连接，随后转化和培养以周质空间为特征的细菌宿主，产生大量的周质成熟蛋白。
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8.发明授权Secretion of correctly processed human growth hormone in E. coli and Pseudomonas 失效在大肠杆菌和假单胞菌中分泌正确加工的人生长激素
公开(公告)号：US4755465A
公开(公告)日：1988-07-05
申请号：US488232
申请日：1983-04-25
IPC分类号： A61K35/74 , A61K38/27 , C07K14/00 , C07K14/575 , C07K14/61 , C12N1/20 , C12N1/21 , C12N15/09 , C12N15/70 , C12N15/78 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/38 , C12N15/00
CPC分类号： C12N15/70 , C07K14/61 , C12N15/78 , Y10S930/12 , Y10S930/30
摘要： The production of mature hGH In E. coli and Pseudomonas strains transformed by a plasmid which encodes pre hGH (comprising the signal polypeptide and the hormone itself) is described. These prokaryotes process the pre-hGH to cleave the signal sequence and, thereby, produce mature hGH.
摘要翻译： 描述了通过编码前hGH（包括信号多肽和激素本身）的质粒转化的大肠杆菌和假单胞菌菌株中的成熟hGH的产生。 这些原核生物处理前hGH以切割信号序列，从而产生成熟的hGH。
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