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31 条记录 (耗时 0.042 秒)

    Promoters for expressing genes in a fungal cell
    3.
    发明授权
    Promoters for expressing genes in a fungal cell 有权
    在真菌细胞中表达基因的启动子

    公开(公告)号：US06361973B1

    公开(公告)日：2002-03-26

    申请号：US09534407

    申请日：2000-03-22

    申请人： Randy M. Berka ,  Michael W. Rey ,  Kimberly Brown ,  Stephen H. Brown

    发明人： Randy M. Berka ,  Michael W. Rey ,  Kimberly Brown ,  Stephen H. Brown

    IPC分类号： C12P2102

    CPC分类号： C12N9/2428 ,  C07K14/37 ,  C12N15/80

    摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the nucleic acid sequence, wherein the promoter comprises a sequence selected from the group consisting of nucleotides 1 to 3949 of SEQ ID NO:1, nucleotides 1 to 938 of SEQ ID NO:2, and nucleotides 1 to 3060 of SEQ ID NO:3, and a subsequence thereof; and mutant, hybrid, and tandem promoters thereof; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated promoter sequences and to constructs, vectors, and fungal host cells comprising the promoter sequences operably linked to nucleic acid sequences encoding polypeptides.

    摘要翻译： 本发明涉及生产多肽的方法，其包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含编码多肽的第一核酸序列，其可操作地连接到 第二核酸序列，其包含核酸序列外源的启动子，其中所述启动子包含选自SEQ ID NO：1的核苷酸1至3949，SEQ ID NO：2的核苷酸1至938和核苷酸的核苷酸 SEQ ID NO：3的1〜3060及其子序列; 及其突变体，杂种和串联启动子; 和（b）从培养基中分离多肽。 本发明还涉及分离的启动子序列和构建体，载体和真菌宿主细胞，其包含可操作地连接到编码多肽的核酸序列的启动子序列。

    Polypeptides having lactonohydrolase activity and nucleic acids encoding same
    8.
    发明授权
    Polypeptides having lactonohydrolase activity and nucleic acids encoding same 失效
    具有乳糖水解酶活性的多肽和编码相同的核酸

    公开(公告)号：US06395529B1

    公开(公告)日：2002-05-28

    申请号：US09434690

    申请日：1999-11-05

    申请人： Randy M. Berka ,  Michael W. Rey

    发明人： Randy M. Berka ,  Michael W. Rey

    IPC分类号： C12N916

    CPC分类号： C12N9/18 ,  C02F1/50 ,  Y10S435/929

    摘要： The present invention relates to isolated polypeptides having lactonohydrolase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides. The present invention further relates to methods for preventing microbial biofilm development.

    摘要翻译： 本发明涉及具有内含子水解酶活性的分离的多肽和分离的编码该多肽的核酸序列。 本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。 本发明还涉及防止微生物生物膜发育的方法。

    Polypeptides having choline oxidase activity and nucleic acids encoding same
    9.
    发明授权
    Polypeptides having choline oxidase activity and nucleic acids encoding same 失效
    具有胆碱氧化酶活性的多肽和编码其的核酸

    公开(公告)号：US06320103B1

    公开(公告)日：2001-11-20

    申请号：US09687298

    申请日：2000-10-12

    申请人： Debbie Yaver ,  Randy M. Berka ,  Michael W. Rey

    发明人： Debbie Yaver ,  Randy M. Berka ,  Michael W. Rey

    IPC分类号： A01H900

    CPC分类号： C12N9/0006

    摘要： The present invention relates to isolated polypeptides having choline oxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

    摘要翻译： 本发明涉及具有胆碱氧化酶活性的分离的多肽和分离的编码多肽的核酸序列。 本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。