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    Immuno-amplification
    1.
    发明授权
    Immuno-amplification 有权
    免疫扩增

    公开(公告)号：US07932060B2

    公开(公告)日：2011-04-26

    申请号：US10826654

    申请日：2004-04-19

    申请人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

    发明人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  C07K1/00

    CPC分类号： C12Q1/6804 ,  C12Q1/6851

    摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

    摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

    Immuno-amplification
    2.
    发明申请
    Immuno-amplification 有权
    免疫扩增

    公开(公告)号：US20050009050A1

    公开(公告)日：2005-01-13

    申请号：US10826654

    申请日：2004-04-19

    申请人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Wang ,  Keith Thornton

    发明人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Wang ,  Keith Thornton

    IPC分类号： C07K20060101 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6804 ,  C12Q1/6851

    摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte. In one embodiment, target-independent amplicon formation is prevented by using hybridization blocker oligonucleotides that bind oligonucleotide moieties that are not hybridized to each other. Background is further reduced by providing a solid phase capture oligonucleotide that prevents amplicon formation until the captured complex is released.

    摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。 在一个实施方案中，通过使用结合彼此不杂交的寡核苷酸部分的杂交阻断剂寡核苷酸来防止靶标无关扩增子形成。 通过提供一种固相捕获寡核苷酸来进一步减少背景，所述寡核苷酸防止扩增子形成，直到所捕获的复合物被释放。

    Diagnostic test for Streptococcus A
    3.
    发明授权
    Diagnostic test for Streptococcus A 失效
    链球菌A的诊断试验

    公开(公告)号：US4618576A

    公开(公告)日：1986-10-21

    申请号：US584175

    申请日：1984-02-27

    申请人： Robert Rosenstein ,  Kim P. Aspden ,  Peter Stopa

    发明人： Robert Rosenstein ,  Kim P. Aspden ,  Peter Stopa

    IPC分类号： C12Q1/04 ,  C12Q1/14 ,  C12Q1/24 ,  G01N33/569 ,  G01N33/53 ,  C12M1/30 ,  C12M1/40

    CPC分类号： G01N33/56944 ,  C12Q1/14 ,  C12Q1/24 ,  G01N2333/315 ,  Y10S435/961 ,  Y10S435/975

    摘要： The presence of Group A Streptococcus in a biological specimen is determined from the presence of Streptococcus A antigen. A biological specimen is collected with an applicator having a plastic stick with a rayon swab. The swab is placed in an extraction reagent containing enzymes produced by the bacterium Streptomyces albus, wherein the enzymes release the antigen from the fiber. An aliquot of the extraction medium is mixed with an indicator reagent containing an antibody reactive with the antigen. The occurrence or non-occurrence of an antibody-antigen reaction is noted which indicates the presence or absence of Group A Streptococcus in the biological specimen.

    摘要翻译： A型链球菌在生物样本中的存在是从A型链球菌抗原的存在确定的。 用具有人造丝拭子的塑料棒的涂抹器收集生物样品。 将拭子放置在含有由细菌链霉菌（Streptomyces albus）产生的酶的提取试剂中，其中酶从纤维中释放抗原。 将提取介质的等分试样与含有与抗原反应的抗体的指示剂混合。 注意到发生或不发生抗体 - 抗原反应，其指示生物样品中存在或不存在A组链球菌。

    Method for Removing Antibiotics From Blood Culture Samples
    4.
    发明申请
    Method for Removing Antibiotics From Blood Culture Samples 审中-公开
    从血培养样品中除去抗生素的方法

    公开(公告)号：US20090123960A1

    公开(公告)日：2009-05-14

    申请号：US12264647

    申请日：2008-11-04

    申请人： Robert Rosenstein ,  P. Shawn Beaty ,  Richard Pfeltz ,  Matthew Warns

    发明人： Robert Rosenstein ,  P. Shawn Beaty ,  Richard Pfeltz ,  Matthew Warns

    IPC分类号： C12Q1/02 ,  C12N1/04 ,  C12M3/00 ,  C12N1/20

    CPC分类号： C12Q1/24 ,  C07K1/20 ,  G01N33/521

    摘要： Methods for removing inhibitors of microbial growth, including antibiotics, from a biological sample suspected of containing one or more microorganisms are provided. The methods include contacting a sample, or a culture growth medium containing the sample, with reversed-phase adsorbent media, which remove the inhibitors of microbial growth, but allow the microorganisms of interest to remain in the sample or culture growth medium.

    摘要翻译： 提供了从怀疑含有一种或多种微生物的生物样品中除去微生物生长抑制剂（包括抗生素）的方法。 所述方法包括将含有样品的样品或培养生长培养基与反相吸附介质接触，所述反相吸附剂介质除去微生物生长抑制剂，但允许感兴趣的微生物保留在样品或培养生长培养基中。

    Combination vertical and lateral flow immunoassay device
    5.
    发明申请
    Combination vertical and lateral flow immunoassay device 审中-公开
    组合垂直和横向流量免疫测定装置

    公开(公告)号：US20070224701A1

    公开(公告)日：2007-09-27

    申请号：US11355669

    申请日：2006-02-16

    申请人： Robert Rosenstein

    发明人： Robert Rosenstein

    IPC分类号： G01N33/558

    CPC分类号： G01N33/558 ,  G01N33/54386

    摘要： The invention relates to immunoassays that detect the presence or absence, or determine the amount present of a particular analyte. More specifically, the invention relates to multizone, multilayered immunoassay test devices that utilize a combination of vertical and lateral flow to detect an analyte of interest.

    摘要翻译： 本发明涉及检测存在或不存在或确定特定分析物存在量的免疫测定。 更具体地，本发明涉及利用垂直和横向流动的组合来检测目标分析物的多区域多层免疫测定装置。

    Immuno-agglutination particle suspensions
    6.
    发明授权
    Immuno-agglutination particle suspensions 失效
    免疫凝集颗粒悬浮液

    公开(公告)号：US4582810A

    公开(公告)日：1986-04-15

    申请号：US537737

    申请日：1983-09-30

    申请人： Robert Rosenstein

    发明人： Robert Rosenstein

    IPC分类号： G01N33/545 ,  A61K39/00 ,  A61K39/385 ,  A61K39/44 ,  G01N33/53 ,  G01N33/543 ,  G01N33/544 ,  G01N33/549

    CPC分类号： G01N33/54313 ,  Y10S436/823 ,  Y10T436/25875

    摘要： A suspension of diagnostic particles comprising antibody molecules attached to a carboxylate derivatized polymer core is provided for agglutination tests. The antibody is linked to the core through an avidin-biotin bridge. Avidin is joined by an amide bond to carboxyl groups on the core, and biotin is linked by an amide bond to amino groups on the antibody molecule. The core-bound antibody is exposed to a mixture of free biotin and biotinylated antibody to attach a controlled amount of antibody that is consistent with suspension stability prior to its use in a test and rapid cross-linking of suspended particles in the presence of antigen.

    摘要翻译： 提供了包含连接到羧酸衍生的聚合物核心上的抗体分子的诊断颗粒的悬浮液用于凝集试验。 抗体通过抗生物素蛋白 - 生物素桥连接到核心。 亲和素通过酰胺键连接在核心上的羧基上，生物素通过酰胺键连接到抗体分子上的氨基上。 将核心结合的抗体暴露于游离生物素和生物素化抗体的混合物，以在受试抗体的存在下在悬浮颗粒的测试和快速交联之前附加受控量的与悬浮液稳定性一致的抗体。

    IMMUNO-AMPLIFICATION
    7.
    发明申请
    IMMUNO-AMPLIFICATION 有权
    免疫放大

    公开(公告)号：US20110244457A1

    公开(公告)日：2011-10-06

    申请号：US13072314

    申请日：2011-03-25

    申请人： James Nadeau ,  Tobin Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

    发明人： James Nadeau ,  Tobin Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6804 ,  C12Q1/6851

    摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

    摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

    Methods for Diagnosing Oncogenic Human Papillomavirus (HPV)
    8.
    发明申请
    Methods for Diagnosing Oncogenic Human Papillomavirus (HPV) 有权
    诊断致癌人乳头瘤病毒（HPV）的方法

    公开(公告)号：US20090269732A1

    公开(公告)日：2009-10-29

    申请号：US12108550

    申请日：2008-04-24

    申请人： Richard Ivey ,  Stephen J. Lovell ,  Robert Rosenstein ,  Thomas Gentle ,  Song Shi

    发明人： Richard Ivey ,  Stephen J. Lovell ,  Robert Rosenstein ,  Thomas Gentle ,  Song Shi

    IPC分类号： C12Q1/70

    CPC分类号： G01N33/56983 ,  G01N2333/025

    摘要： Methods for diagnosis of HPV infection in a subject are provided. HPV infection in a subject can be determined by generating mass profile data for a biological sample from the subject and correlating the mass profile data with reference mass profiles to detect the presence or absence, and/or quantity of at least one biomarker associated with HPV infection. Methods for detecting at least one biomarker associated with HPV infection in a biological sample are also provided.

    摘要翻译： 提供了一种用于诊断受试者HPV感染的方法。 可以通过产生来自受试者的生物样品的质量分布数据来确定受试者中的HPV感染，并将质量分布数据与参考质量分布相关联，以检测与HPV感染有关的至少一种生物标志物的存在或不存在和/或数量 。 还提供了用于检测生物样品中与HPV感染相关联的至少一种生物标志物的方法。