公开(公告)号：US20110244457A1

公开(公告)日：2011-10-06

申请号：US13072314

申请日：2011-03-25

申请人： James Nadeau ,  Tobin Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

发明人： James Nadeau ,  Tobin Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6804 ,  C12Q1/6851

摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

公开(公告)号：US07932060B2

公开(公告)日：2011-04-26

申请号：US10826654

申请日：2004-04-19

申请人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

发明人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

IPC分类号： C12P19/34 ,  C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  C07K1/00

CPC分类号： C12Q1/6804 ,  C12Q1/6851

摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

公开(公告)号：US08372605B2

公开(公告)日：2013-02-12

申请号：US13072314

申请日：2011-03-25

申请人： James Nadeau ,  Tobin J. Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha-Sha Wang ,  Keith Edward Thornton

发明人： James Nadeau ,  Tobin J. Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha-Sha Wang ,  Keith Edward Thornton

IPC分类号： C12P19/34 ,  C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  C07K16/00

CPC分类号： C12Q1/6804 ,  C12Q1/6851

摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

公开(公告)号：US20050009050A1

公开(公告)日：2005-01-13

申请号：US10826654

申请日：2004-04-19

申请人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Wang ,  Keith Thornton

发明人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Wang ,  Keith Thornton

IPC分类号： C07K20060101 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6804 ,  C12Q1/6851

摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte. In one embodiment, target-independent amplicon formation is prevented by using hybridization blocker oligonucleotides that bind oligonucleotide moieties that are not hybridized to each other. Background is further reduced by providing a solid phase capture oligonucleotide that prevents amplicon formation until the captured complex is released.

摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。 在一个实施方案中，通过使用结合彼此不杂交的寡核苷酸部分的杂交阻断剂寡核苷酸来防止靶标无关扩增子形成。 通过提供一种固相捕获寡核苷酸来进一步减少背景，所述寡核苷酸防止扩增子形成，直到所捕获的复合物被释放。

公开(公告)号：US20080050829A1

公开(公告)日：2008-02-28

申请号：US11647689

申请日：2006-12-28

申请人： Richard Ivey ,  Thomas Gentle ,  Richard Moore ,  Michael Towns ,  Nicholas Bachur ,  Robert Rosenstein ,  James Nadeau ,  Paul Goldenbaum ,  Song Shi ,  Donald Copertino ,  James Garrett ,  Gregory Tice ,  Gary Siuzdak ,  Elizabeth Want ,  Zhouxin Shen

发明人： Richard Ivey ,  Thomas Gentle ,  Richard Moore ,  Michael Towns ,  Nicholas Bachur ,  Robert Rosenstein ,  James Nadeau ,  Paul Goldenbaum ,  Song Shi ,  Donald Copertino ,  James Garrett ,  Gregory Tice ,  Gary Siuzdak ,  Elizabeth Want ,  Zhouxin Shen

IPC分类号： G01N33/00 ,  C12N1/02

CPC分类号： G01N33/6848 ,  C12Q1/6883 ,  G01N33/6803 ,  G01N2800/26 ,  Y10T436/24

摘要： Mass spectrometry techniques for determining the status of sepsis in an individual are provided. A biomarker profile resolved from a biological sample, taken from the individual, using a mass spectrometry technique is compared to a reference biomarker profile. A single such comparison classifies the individual as belonging to or not belonging to a reference population. The individual's biomarker profile and the reference biomarker profile comprise a plurality of ions each having a mass-to-charge ratio of about 100 Daltons to about 1000 Daltons. The plurality of ions can be detected by electrospray ionization mass spectrometry in positive mode. The comparison uses a decision rule, such as a classification tree, that determines the status of sepsis in the individual without requiring knowledge of the identity of the biomarkers in the biomarker profile from the individual and without requiring knowledge of the identity of the biomarkers in the reference biomarker profile.

摘要翻译： 提供了用于确定个体败血症状态的质谱技术。 将使用质谱技术从个体获取的生物样品分解的生物标志物谱与参考生物标志物谱进行比较。 单个这样的比较将个体归类为属于或不属于参考群体。 个体的生物标志物概况和参考生物标志物谱包括多个离子，其各自具有约100道尔顿至约1000道尔顿的质荷比。 可以通过电喷雾电离质谱法以正模式检测多个离子。 比较使用决定规则，例如分类树，其确定个体中败血症的状态，而不需要知道来自个体的生物标志物概况中的生物标志物的身份，并且不需要知道生物标志物的身份 参考生物标志物概况。

公开(公告)号：US4618576A

公开(公告)日：1986-10-21

申请号：US584175

申请日：1984-02-27

申请人： Robert Rosenstein ,  Kim P. Aspden ,  Peter Stopa

发明人： Robert Rosenstein ,  Kim P. Aspden ,  Peter Stopa

IPC分类号： C12Q1/04 ,  C12Q1/14 ,  C12Q1/24 ,  G01N33/569 ,  G01N33/53 ,  C12M1/30 ,  C12M1/40

CPC分类号： G01N33/56944 ,  C12Q1/14 ,  C12Q1/24 ,  G01N2333/315 ,  Y10S435/961 ,  Y10S435/975

摘要： The presence of Group A Streptococcus in a biological specimen is determined from the presence of Streptococcus A antigen. A biological specimen is collected with an applicator having a plastic stick with a rayon swab. The swab is placed in an extraction reagent containing enzymes produced by the bacterium Streptomyces albus, wherein the enzymes release the antigen from the fiber. An aliquot of the extraction medium is mixed with an indicator reagent containing an antibody reactive with the antigen. The occurrence or non-occurrence of an antibody-antigen reaction is noted which indicates the presence or absence of Group A Streptococcus in the biological specimen.

摘要翻译： A型链球菌在生物样本中的存在是从A型链球菌抗原的存在确定的。 用具有人造丝拭子的塑料棒的涂抹器收集生物样品。 将拭子放置在含有由细菌链霉菌（Streptomyces albus）产生的酶的提取试剂中，其中酶从纤维中释放抗原。 将提取介质的等分试样与含有与抗原反应的抗体的指示剂混合。 注意到发生或不发生抗体 - 抗原反应，其指示生物样品中存在或不存在A组链球菌。

公开(公告)号：US20090123960A1

公开(公告)日：2009-05-14

申请号：US12264647

申请日：2008-11-04

申请人： Robert Rosenstein ,  P. Shawn Beaty ,  Richard Pfeltz ,  Matthew Warns

发明人： Robert Rosenstein ,  P. Shawn Beaty ,  Richard Pfeltz ,  Matthew Warns

IPC分类号： C12Q1/02 ,  C12N1/04 ,  C12M3/00 ,  C12N1/20

CPC分类号： C12Q1/24 ,  C07K1/20 ,  G01N33/521

摘要： Methods for removing inhibitors of microbial growth, including antibiotics, from a biological sample suspected of containing one or more microorganisms are provided. The methods include contacting a sample, or a culture growth medium containing the sample, with reversed-phase adsorbent media, which remove the inhibitors of microbial growth, but allow the microorganisms of interest to remain in the sample or culture growth medium.

摘要翻译： 提供了从怀疑含有一种或多种微生物的生物样品中除去微生物生长抑制剂（包括抗生素）的方法。 所述方法包括将含有样品的样品或培养生长培养基与反相吸附介质接触，所述反相吸附剂介质除去微生物生长抑制剂，但允许感兴趣的微生物保留在样品或培养生长培养基中。

公开(公告)号：US20070224701A1

公开(公告)日：2007-09-27

申请号：US11355669

申请日：2006-02-16

申请人： Robert Rosenstein

发明人： Robert Rosenstein

IPC分类号： G01N33/558

CPC分类号： G01N33/558 ,  G01N33/54386

摘要： The invention relates to immunoassays that detect the presence or absence, or determine the amount present of a particular analyte. More specifically, the invention relates to multizone, multilayered immunoassay test devices that utilize a combination of vertical and lateral flow to detect an analyte of interest.

摘要翻译： 本发明涉及检测存在或不存在或确定特定分析物存在量的免疫测定。 更具体地，本发明涉及利用垂直和横向流动的组合来检测目标分析物的多区域多层免疫测定装置。

公开(公告)号：US4582810A

公开(公告)日：1986-04-15

申请号：US537737

申请日：1983-09-30

申请人： Robert Rosenstein

发明人： Robert Rosenstein

IPC分类号： G01N33/545 ,  A61K39/00 ,  A61K39/385 ,  A61K39/44 ,  G01N33/53 ,  G01N33/543 ,  G01N33/544 ,  G01N33/549

CPC分类号： G01N33/54313 ,  Y10S436/823 ,  Y10T436/25875

摘要： A suspension of diagnostic particles comprising antibody molecules attached to a carboxylate derivatized polymer core is provided for agglutination tests. The antibody is linked to the core through an avidin-biotin bridge. Avidin is joined by an amide bond to carboxyl groups on the core, and biotin is linked by an amide bond to amino groups on the antibody molecule. The core-bound antibody is exposed to a mixture of free biotin and biotinylated antibody to attach a controlled amount of antibody that is consistent with suspension stability prior to its use in a test and rapid cross-linking of suspended particles in the presence of antigen.

摘要翻译： 提供了包含连接到羧酸衍生的聚合物核心上的抗体分子的诊断颗粒的悬浮液用于凝集试验。 抗体通过抗生物素蛋白 - 生物素桥连接到核心。 亲和素通过酰胺键连接在核心上的羧基上，生物素通过酰胺键连接到抗体分子上的氨基上。 将核心结合的抗体暴露于游离生物素和生物素化抗体的混合物，以在受试抗体的存在下在悬浮颗粒的测试和快速交联之前附加受控量的与悬浮液稳定性一致的抗体。

公开(公告)号：US20090269732A1

公开(公告)日：2009-10-29

申请号：US12108550

申请日：2008-04-24

申请人： Richard Ivey ,  Stephen J. Lovell ,  Robert Rosenstein ,  Thomas Gentle ,  Song Shi

发明人： Richard Ivey ,  Stephen J. Lovell ,  Robert Rosenstein ,  Thomas Gentle ,  Song Shi

IPC分类号： C12Q1/70

CPC分类号： G01N33/56983 ,  G01N2333/025

摘要： Methods for diagnosis of HPV infection in a subject are provided. HPV infection in a subject can be determined by generating mass profile data for a biological sample from the subject and correlating the mass profile data with reference mass profiles to detect the presence or absence, and/or quantity of at least one biomarker associated with HPV infection. Methods for detecting at least one biomarker associated with HPV infection in a biological sample are also provided.

摘要翻译： 提供了一种用于诊断受试者HPV感染的方法。 可以通过产生来自受试者的生物样品的质量分布数据来确定受试者中的HPV感染，并将质量分布数据与参考质量分布相关联，以检测与HPV感染有关的至少一种生物标志物的存在或不存在和/或数量 。 还提供了用于检测生物样品中与HPV感染相关联的至少一种生物标志物的方法。