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    Immuno-amplification
    1.
    发明授权
    Immuno-amplification 有权
    免疫扩增

    公开(公告)号：US07932060B2

    公开(公告)日：2011-04-26

    申请号：US10826654

    申请日：2004-04-19

    申请人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

    发明人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  C07K1/00

    CPC分类号： C12Q1/6804 ,  C12Q1/6851

    摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

    摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

    Immuno-amplification
    2.
    发明申请
    Immuno-amplification 有权
    免疫扩增

    公开(公告)号：US20050009050A1

    公开(公告)日：2005-01-13

    申请号：US10826654

    申请日：2004-04-19

    申请人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Wang ,  Keith Thornton

    发明人： James Nadeau ,  Tobin Hellyer ,  Dolores Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Wang ,  Keith Thornton

    IPC分类号： C07K20060101 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6804 ,  C12Q1/6851

    摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte. In one embodiment, target-independent amplicon formation is prevented by using hybridization blocker oligonucleotides that bind oligonucleotide moieties that are not hybridized to each other. Background is further reduced by providing a solid phase capture oligonucleotide that prevents amplicon formation until the captured complex is released.

    摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。 在一个实施方案中，通过使用结合彼此不杂交的寡核苷酸部分的杂交阻断剂寡核苷酸来防止靶标无关扩增子形成。 通过提供一种固相捕获寡核苷酸来进一步减少背景，所述寡核苷酸防止扩增子形成，直到所捕获的复合物被释放。

    IMMUNO-AMPLIFICATION
    3.
    发明申请
    IMMUNO-AMPLIFICATION 有权
    免疫放大

    公开(公告)号：US20110244457A1

    公开(公告)日：2011-10-06

    申请号：US13072314

    申请日：2011-03-25

    申请人： James Nadeau ,  Tobin Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

    发明人： James Nadeau ,  Tobin Hellyer ,  Dolores M. Berger ,  William Nussbaumer ,  Robert Rosenstein ,  Andrew Kuhn ,  Sha Sha Wang ,  Keith Thornton

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6804 ,  C12Q1/6851

    摘要： A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.

    摘要翻译： 提供了高灵敏度，低背景的免疫扩增测定法，其提供流线型工作流程，适用于临床相关样品如血液和其他体液的高通量测定。 该测定包括使用两个邻近成员，每个邻近成员包含与寡核苷酸缀合的分析物特异性结合成分。 结合分析物使邻近成员的寡核苷酸部分充分紧密接触，使寡核苷酸形成扩增子。 然后通过扩增扩增子和扩增核酸的检测来检测分析物的存在。 通过防止与分析物不复合的邻近成员形成假的或非特异性的扩增子来提高本发明的测定的灵敏度。

    Probes and methods for detection of nucleic acids
    4.
    发明申请
    Probes and methods for detection of nucleic acids 审中-公开
    用于检测核酸的探针和方法

    公开(公告)号：US20050239084A1

    公开(公告)日：2005-10-27

    申请号：US10796661

    申请日：2004-03-08

    申请人： James Nadeau ,  Tobin Hellyer

    发明人： James Nadeau ,  Tobin Hellyer

    IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566 ,  G01N37/00

    CPC分类号： C12Q1/6818 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q2565/549 ,  C12Q2561/101 ,  C12Q2525/191

    摘要： The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang, Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

    摘要翻译： 本发明采用包含5'衔接子序列的未标记信号引物来检测核酸靶序列。 该检测系统还包括报道探针，其3'末端与信号引物的5'衔接子序列的互补序列杂交以产生5'突出端，聚合酶用于填充突出端并合成 5'突出的记者探测。 直接或间接检测报道探针补体的合成，作为目标存在的指示。

    PROCESS CONTROLS FOR MOLECULAR ASSAY
    7.
    发明申请
    PROCESS CONTROLS FOR MOLECULAR ASSAY 有权
    用于分子测定的过程控制

    公开(公告)号：US20120282605A1

    公开(公告)日：2012-11-08

    申请号：US13417025

    申请日：2012-03-09

    申请人： Benoit Dore ,  Christian Menard ,  Tobin Hellyer

    发明人： Benoit Dore ,  Christian Menard ,  Tobin Hellyer

    IPC分类号： C12Q1/68 ,  G01N21/64

    CPC分类号： C12Q1/689 ,  C12N1/06 ,  C12Q1/6806

    摘要： A full process control for use with a molecular assay and a method of determine the efficacy of the molecular assay. A full process control can include a fixed cell, and specifically can include a fixed vegetative cell. A method of determining the efficacy of a molecular assay can include providing an internal control, mixing the internal control with a sample, lysing the internal control and the sample, and detecting the lysis product. The full process control and/or the internal control can be Bacillus subtilis cells.

    摘要翻译： 用于分子测定的全过程控制和确定分子测定的功效的方法。 完整的过程控制可以包括固定的细胞，并且具体可以包括固定的营养细胞。 确定分子测定的功效的方法可以包括提供内部对照，将内部对照与样品混合，裂解内部对照和样品，以及检测裂解产物。 完整的过程控制和/或内部控制可以是枯草芽孢杆菌细胞。