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15 条记录 (耗时 0.043 秒)

    Emission ratiometric indicators of phosphorylation
    1.
    发明授权
    Emission ratiometric indicators of phosphorylation 失效
    磷酸化的排放比例指标

    公开(公告)号：US06900304B2

    公开(公告)日：2005-05-31

    申请号：US09865291

    申请日：2001-05-24

    申请人： Roger Y. Tsien ,  Alice Y. Ting ,  Jin Zhang

    发明人： Roger Y. Tsien ,  Alice Y. Ting ,  Jin Zhang

    IPC分类号： G01N21/78 ,  C07K14/435 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/12 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/48 ,  G01N33/542 ,  C07H21/04 ,  C12N15/62

    CPC分类号： C07K14/43595 ,  C07K2319/00 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/542 ,  G01N2333/43595

    摘要： A chimeric phosphorylation indicator is provided. A chimeric phosphorylation indicator can contain a donor molecule, a phosphorylatable domain, a phosphoaminoacid binding domain (PAABD), and an acceptor molecule. A chimeric phosphorylation indicator also can contain a phosphorylatable polypeptide and a fluorescent protein, wherein the phosphorylatable polypeptide is contained within the sequence of the fluorescent protein, or wherein the fluorescent protein is contained within the sequence of the phosphorylatable polypeptide. Also provided are polynucleotides encoding such chimeric phosphorylation indicators, as well as kits containing the indicators or the polynucleotides. In addition, a method of using the chimeric phosphorylation indicators to detect a kinase or phosphatase in a sample is provided.

    摘要翻译： 提供嵌合磷酸化指示剂。 嵌合磷酸化指示剂可以含有供体分子，可磷酸化结构域，磷酸氨基酸结合结构域（PAABD）和受体分子。 嵌合磷酸化指示剂还可含有可磷酸化多肽和荧光蛋白，其中可磷酸化多肽包含在荧光蛋白的序列内，或其中荧光蛋白包含在可磷酸化多肽的序列内。 还提供了编码这种嵌合磷酸化指示剂的多核苷酸，以及含有指示剂或多核苷酸的试剂盒。 此外，提供了使用嵌合磷酸化指示剂来检测样品中的激酶或磷酸酶的方法。

    Cytosolically-active peroxidases as reporters for microscopy
    2.
    发明授权
    Cytosolically-active peroxidases as reporters for microscopy 有权

    公开(公告)号：US09663815B2

    公开(公告)日：2017-05-30

    申请号：US14345309

    申请日：2012-09-14

    申请人： Alice Y. Ting ,  Jeffrey Daniel Martell

    发明人： Alice Y. Ting ,  Jeffrey Daniel Martell

    IPC分类号： C12Q1/28 ,  G01N23/04

    CPC分类号： C12Q1/28 ,  C12Y111/01011 ,  G01N23/04

    摘要： An imaging method comprising expressing in cells a Class I heme peroxidase, which optionally is fused with a protein of interest or a cellular localization signal peptide, and contacting the cells with a substrate of the Class I heme peroxidase to allow conversion of the substrate into a product via an oxidation reaction catalyzed by the Class I heme peroxidase, wherein the product releases a signal detectable by a microscope such as an electron microscope. Also disclosed herein are monomeric mutants of a Class I heme peroxidase and mutants of the enzyme that exhibit elevated enzymatic activity as compared to the corresponding wild-type counterpart.

    CYTOSOLICALLY-ACTIVE PEROXIDASES AS REPORTERS FOR MICROSCOPY
    4.
    发明申请
    CYTOSOLICALLY-ACTIVE PEROXIDASES AS REPORTERS FOR MICROSCOPY 有权
    作为微阵列记录的CYPOSOLICALY-ACTIVE PEROXIDASES

    公开(公告)号：US20150037829A1

    公开(公告)日：2015-02-05

    申请号：US14345309

    申请日：2012-09-14

    申请人： Alice Y. Ting ,  Jeffrey Daniel Martell

    发明人： Alice Y. Ting ,  Jeffrey Daniel Martell

    IPC分类号： C12Q1/28 ,  G01N23/04

    CPC分类号： C12Q1/28 ,  C12Y111/01011 ,  G01N23/04

    摘要： An imaging method comprising expressing in cells a Class I heme peroxidase, which optionally is fused with a protein of interest or a cellular localization signal peptide, and contacting the cells with a substrate of the Class I heme peroxidase to allow conversion of the substrate into a product via an oxidation reaction catalyzed by the Class I heme peroxidase, wherein the product releases a signal detectable by a microscope such as an electron microscope. Also disclosed herein are monomeric mutants of a Class I heme peroxidase and mutants of the enzyme that exhibit elevated enzymatic activity as compared to the corresponding wild-type counterpart.

    摘要翻译： 一种成像方法，其包括在细胞中表达I类血红素过氧化物酶，其任选地与感兴趣的蛋白质或细胞定位信号肽融合，并使细胞与I类血红素过氧化物酶的底物接触以允许将底物转化为 通过由I类血红素过氧化物酶催化的氧化反应产物，其中产物释放通过显微镜如电子显微镜可检测的信号。 本文还公开了I类血红素过氧化物酶的单体突变体和与相应的野生型对应物相比表现出升高的酶活性的酶的突变体。

    SPLIT PEROXIDASES AND METHODS OF USE
    5.
    发明申请
    SPLIT PEROXIDASES AND METHODS OF USE 有权
    分离过氧化物和使用方法

    公开(公告)号：US20140206013A1

    公开(公告)日：2014-07-24

    申请号：US14157281

    申请日：2014-01-16

    申请人： Alice Y. Ting ,  Jeffrey Daniel Martell

    发明人： Alice Y. Ting ,  Jeffrey Daniel Martell

    IPC分类号： G01N33/58 ,  C12N9/08 ,  C12Q1/28

    CPC分类号： C12N9/0065 ,  C12Q1/28 ,  G01N33/581

    摘要： An imaging method utilizing a split peroxidase is described herein. Imaging methods involve contacting a cell with a split peroxidase and a substrate thereof to allow conversion of a substrate into a product via an enzymatic reaction catalyzed by the reconstitute split peroxidase. Also disclosed herein are split peroxidases, related products and kits.

    摘要翻译： 本文描述了利用裂解过氧化物酶的成像方法。 成像方法包括使细胞与裂解的过氧化物酶及其底物接触，以通过由重构的裂解过氧化物酶催化的酶反应将底物转化成产物。 本文还公开了分离的过氧化物酶，相关产物和试剂盒。

    Methods and compositions for peptide and protein labeling
    7.
    发明授权
    Methods and compositions for peptide and protein labeling 失效
    用于肽和蛋白质标记的方法和组合物

    公开(公告)号：US07172877B2

    公开(公告)日：2007-02-06

    申请号：US10754911

    申请日：2004-01-09

    申请人： Alice Y. Ting

    发明人： Alice Y. Ting

    IPC分类号： C12Q1/25 ,  C40B30/04

    CPC分类号： C12N9/93 ,  G01N33/582 ,  G01N2333/9015

    摘要： The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ biotin ligase mutants and biotin analogs recognized by such mutants.

    摘要翻译： 本发明提供了用于在体外或体内标记肽和蛋白质的组合物及其使用方法。 本文描述的方法使用由这种突变体识别的生物素连接酶突变体和生物素类似物。